LOCUS       pcDNA5/FRT/TO_-_Exon1-6       11867 bp    DNA     circular UNA 04-SEP-2019
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...mt-af4ee93
VERSION     urn.local...mt-af4ee93
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>988,<7777..11867)
                     /organism="pcDNA5/FRT/TO"
                     /mol_type="other DNA"
                     /label="source pcDNA5/FRT/TO"
     promoter        236..812
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     primer_bind     743..762
                     /Sequence="ATGTCGTAACAACTCCGCCC"
                     /Hairpin_Tm="39.3"
                     /Tm="60.4"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=539
                     /modified_by="Carson J Bryant"
                     /label="CMV_seq"
     primer_bind     769..789
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Hairpin_Tm="52.4"
                     /Tm="68.1"
                     /Self_Dimer_Tm="3.5"
                     /%GC="66.7"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="CMV_fwd_primer"
     promoter        770..893
                     /label="CMV_promoter"
     promoter        782..955
                     /label="CMV2_promoter"
     misc_feature    820..859
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="tetO reg"
                     /Transferred_Similarity="100.00%"
                     /modified_by="Carson J Bryant"
                     /label="tetO reg (2X)"
                     /note="Geneious type: Regulatory Sequence"
     primer_bind     869..893
                     /Sequence="AGCTCGTTTAGTGAACCGTCAGATC"
                     /Hairpin_Tm="33.3"
                     /Tm="63.2"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="LNCX_primer"
     TSS             886
                     /created_by="Carson J Bryant"
                     /label="TSS +1"
     primer_bind     928..947
                     /Sequence="ATAGAAGACACCGGGACCGA"
                     /annotation_group="14978805611616654461fa97-724e-4d1e-ac2d
                     -88bb32666190urn:sequence:genbank:null"
                     /modified_by="Carson J Bryant"
                     /Hairpin_Tm="42.8"
                     /Tm="60.0"
                     /Self_Dimer_Tm="13.1"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=203
                     /label="p5_ins_seqF"
     misc_feature    985..7780
                     /Source="referenceToDocument: urn:local:.:1z-adlr6uf"
                     /label="RBM28_g/Exon1-6 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <985..989
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        985..988
                     /label="Ligation"
     Junction        join(988,996)
                     /Reads_using=1
                     /Reads_supporting_discovery=1
                     /Junction_Type="Deletion"
                     /Deletion_Size=7
                     /Junction_Source="988+"
                     /Junction_Destination="996+"
                     /ID="junction 1"
                     /label="junction 1"
     mRNA            join(997..1229,5114..5272,5584..5678,6104..6179,
                     6563..6655,7703..7774)
                     /gene="RBM28"
                     /gene_synonym="ANES"
                     /product="RNA binding motif protein 28, transcript
                     variant1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /transcript_id="NM_018077.2"
                     /db_xref="GeneID:55131"
                     /db_xref="HGNC:HGNC:21863"
                     /db_xref="MIM:612074"
                     /label="RBM28 mRNA"
     primer_bind     997..1015
                     /Sequence="ACTTCCGGAATCTCTCGGC"
                     /Hairpin_Tm="45.3"
                     /Tm="60.1"
                     /Self_Dimer_Tm="19.2"
                     /%GC="57.9"
                     /created_by="primer3"
                     /Extension="ACTGGGTACC"
                     /modified_by="Carson J Bryant"
                     /label="E1-5_KpnI_F"
     CDS             join(1112..1229,5114..5272,5584..5678,6104..6179,
                     6563..6655,7703..7774)
                     /gene="RBM28"
                     /gene_synonym="ANES"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Derived by automated computational analysis using gene
                     prediction method: BestRefSeq."
                     /codon_start=1
                     /product="RNA-binding protein 28 isoform 1"
                     /protein_id="NP_060547.2"
                     /db_xref="GI:187960109"
                     /db_xref="CCDS:CCDS5801.1"
                     /db_xref="GeneID:55131"
                     /db_xref="HGNC:HGNC:21863"
                     /db_xref="MIM:612074"
                     /translation="MAGLTLFVGRLPPSARSEQLEELFSQVGPVKQCFVVTEKGSKAC
                     RGFGYVTFSMLEDVQRALKEITTFEGCKINVTVAKKKLRNKTKEKGKNENSECPKKEP
                     KAKKAKVADKKARLIIRNLSFKCSEDDLKTVFAQFGAVLEVNIPRKPDGKMRGFGFVQ
                     FKNLLEAGKALKGMNMKEIKGRTVAVDWAVAKDKYKDTQSVSAIGEEKSHESKHQESV
                     KKKGREEEDMEEEENDDDDDDDDEEDGVFDDEDEEEENIESKVTKPVQIQKRAVKRPA
                     PAKSSDHSEEDSDLEESDSIDDGEELAQSDTSTEEQEDKAVQVSNKKKRKLPSDVNEG
                     KTVFIRNLSFDSEEEELGELLQQFGELKYVRIVLHPDTEHSKGCAFAQFMTQEAAQKC
                     LLAASPENEAGGLKLDGRQLKVDLAVTRDEAAKLQTTKVKKPTGTRNLYLAREGLIRA
                     GTKAAEGVSAADMAKRERFELLKHQKLKDQNIFVSRTRLCLHNLPKAVDDKQLRKLLL
                     SATSGEKGVRIKECRVMRDLKGVHGNMKGQSLGYAFAEFQEHEHALKALRLINNNPEI
                     FGPLKRPIVEFSLEDRRKLKMKELRIQRSLQKMRSKPATGEPQKGQPEPAKDQQQKAA
                     QHHTEEQSKVPPEQKRKAGSTSWTGFQTKAEVEQVELPDGKKRRKVLALPSHRGPKIR
                     LRDKGKVKPVHPKKPKPQINQWKQEKQQLSSEQVSRKKAKGNKTETRFNQLVEQYKQK
                     LLGPSKGAPLAKRSKWFDS"
                     /label="RBM28 CDS"
     primer_bind     1205..1224
                     /Sequence="CAGTGCTTCGTGGTGACTGA"
                     /Hairpin_Tm="36.9"
                     /Tm="60.2"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /modified_by="Carson J Bryant"
                     /label="E1_int_seqF"
     misc_feature    1230..1250
                     /created_by="User"
                     /modified_by="User"
                     /label="Splice Site 5A COSSMO PSI = 0.06856"
     primer_bind     complement(1511..1531)
                     /Sequence="ACTGATCACTGTACCTGCCAA"
                     /Hairpin_Tm="None"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="E1_seqR"
     misc_feature    2086..2108
                     /created_by="User"
                     /modified_by="User"
                     /label="Splice Site 5B COSSMO PSI = 0.01183"
     misc_feature    2429..2434
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    3616..3636
                     /created_by="User"
                     /modified_by="User"
                     /label="Splice Site 5C COSSMO PSI = 0.048515"
     misc_feature    3788..3807
                     /created_by="User"
                     /label="Splice Site 5D COSSMO PSI = 0.02278"
     primer_bind     5047..5069
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.5"
                     /annotation_group="71974715639180823dd33e-e7cd-4a4d-8cc5-4
                     e2da9506b1b"
                     /Sequence="TCTTGGCTTATGATTCTCTCTCC"
                     /created_by="primer3"
                     /label="E2_seq_F"
     primer_bind     5154..5175
                     /Sequence="AATGCTGGAAGATGTTCAGAGG"
                     /Hairpin_Tm="34.1"
                     /Tm="58.8"
                     /Self_Dimer_Tm="4.8"
                     /%GC="45.5"
                     /modified_by="Carson J Bryant"
                     /label="E2_int_seqF"
     misc_feature    5204..5224
                     /created_by="User"
                     /modified_by="User"
                     /label="Splice Site 5E COSSMO PSI = 0.088146"
     misc_feature    5273..5292
                     /created_by="User"
                     /label="Splice Site 5F COSSMO PSI = 0.02423"
     misc_feature    5679..5698
                     /created_by="User"
                     /label="Splice Site 5G COSSMO PSI = 0.01043"
     primer_bind     complement(5824..5843)
                     /Sequence="TGCCCTTATTACACAGGCCC"
                     /Hairpin_Tm="43.2"
                     /Tm="59.7"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /modified_by="Carson J Bryant"
                     /label="E3_seqR"
     misc_feature    6180..6200
                     /created_by="User"
                     /modified_by="User"
                     /label="Splice Site 5H COSSMO PSI = 0.02670"
     primer_bind     complement(6196..6217)
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.4"
                     /annotation_group="2131717811045206bc975d19-4817-4ee5-9e42
                     -974fcc9173a7"
                     /Sequence="ACTAACGTAATCCACACCAAGT"
                     /created_by="primer3"
                     /label="RBM28_E4_seq_R"
     primer_bind     6379..6398
                     /Mismatches=0
                     /Extension="GATTTAGGTGACACTATAG"
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="21312840411826871c10cfb2-1cd1-4c7b-aee0
                     -42207a87e8e6"
                     /Sequence="GCTCTCCCCAAGTCCAAGTC"
                     /created_by="primer3"
                     /label="RBM28_E5_seq"
     misc_feature    6421..6440
                     /created_by="User"
                     /label="Splice Site 5I COSSMO PSI = 0.02261"
     misc_feature    6614..6624
                     /note="Splice Site NNSplice=0.91"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.91"
     misc_feature    6649..6663
                     /created_by="User"
                     /modified_by="User"
                     /label="Splice Site 5WT NNSplice = 1.00 COSSMO PSI =
                     0.51286"
     misc_feature    6654..6656
                     /ugene_name="PAM"
                     /label="misc"
     misc_feature    6656..6657
                     /note="del ins A"
                     /ugene_name="Mutation"
                     /label="del ins A"
     PTC             6706..6708
                     /created_by="User"
                     /modified_by="User"
                     /label="Exon 5 PTC"
     misc_feature    6773..6787
                     /note="Splice Site NNSplice=0.40"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.40"
     primer_bind     complement(6799..6821)
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="71974844945209b790530d-fb1d-4e05-bfe7-6
                     2a28db41a95"
                     /Sequence="ACCCCAAGATCAAGTTCTAGAGT"
                     /created_by="primer3"
                     /label="E5_seq_R"
     misc_feature    6857..6871
                     /note="Splice Site NNSplice=0.92"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.92"
     misc_feature    6920..6934
                     /note="Splice Site NNSplice=0.79"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.79"
     misc_feature    7044..7058
                     /note="Splice Site NNSplice=0.76"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.76"
     misc_feature    7110..7124
                     /note="Splice Site NNSplice=0.66"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.66"
     misc_feature    7182..7196
                     /note="Splice Site NNSplice=0.74"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.74"
     misc_feature    7370..7384
                     /note="Splice Site NNSplice=0.93"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /modified_by="User"
                     /label="Splice Site 5K COSSMO PSI = 0.05968 NNSplice=0.93"
     primer_bind     7372..7391
                     /Sequence="TCCAGGTAGGGTGCAGATGA"
                     /Hairpin_Tm="38.2"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /modified_by="Carson J Bryant"
                     /label="i5_seqF"
     misc_feature    7469..7483
                     /note="Splice Site NNSplice=0.77"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.77"
     misc_feature    7645..7659
                     /note="Splice Site NNSplice=0.94"
                     /ugene_name="Misc.\ Feature"
                     /ugene_group="NNSplice\ Predictions"
                     /label="Splice Site NNSplice=0.94"
     primer_bind     complement(7750..7774)
                     /Sequence="CTATAGCAGAAACAGACTGTGTATC"
                     /Hairpin_Tm="39.0"
                     /Tm="57.1"
                     /Self_Dimer_Tm="9.3"
                     /%GC="40.0"
                     /created_by="primer3"
                     /Extension="ACTGGCGGCCGC"
                     /label="E1-5_NotI_R"
     Insert          <7775..>7780
                     /created_by="Carson J Bryant"
                     /label="Insert"
     misc_feature    7775..>7780
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     source          <7775..>7780
                     /organism="pcDNA5/FRT/TO"
                     /mol_type="other DNA"
                     /label="source pcDNA5/FRT/TO"
     misc_feature    7777..988
                     /Source="referenceToDocument: urn:local:.:f-9y9qyq6"
                     /label="pcDNA5/FRT/TO (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        7777..7780
                     /label="Ligation"
     misc_feature    7782..7787
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    7782..7787
                     /Recognition_pattern="C^YCGRG"
                     /annotation_group="CYCGRG"
                     /label="AvaI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(7817..7836)
                     /Mismatches=0
                     /%GC="55.0"
                     /Hairpin_Tm="40.4"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="663686171913849a3abee9e-e849-4aeb-ab61-
                     09084bd162b3"
                     /Sequence="TAGAAGGCACAGTCGAGGCT"
                     /created_by="primer3"
                     /label="BGH_R_2"
     primer_bind     complement(7819..7836)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Hairpin_Tm="None"
                     /Tm="56.5"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="BGH_rev_primer"
     terminator      7822..8049
                     /label="bGH_PA_terminator"
     primer_bind     complement(7841..7860)
                     /Sequence="GGCAAACAACAGATGGCTGG"
                     /Hairpin_Tm="46.6"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=203
                     /annotation_group="14978805611616654461fa97-724e-4d1e-ac2d
                     -88bb32666190urn:sequence:genbank:null"
                     /modified_by="Carson J Bryant"
                     /label="p5_ins_seqR"
     polyA_signal    7915..7920
                     /created_by="Carson J Bryant"
                     /modified_by="Carson J Bryant"
                     /label="PolyA_signal"
     Gene            8333..8380
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /label="FRT"
     gene            8400..9407
                     /gene="hygroB"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFGQGSSYTF*"
                     /label="hygroB"
     terminator      9543..9662
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="SV40_PA_terminator"
     primer_bind     9631..9650
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /Sequence="GTGGTTTGTCCAAACTCATC"
                     /Hairpin_Tm="48.9"
                     /Tm="55.0"
                     /Self_Dimer_Tm="19.2"
                     /%GC="45.0"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="EBV_rev_primer"
     primer_bind     complement(9706..9724)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /Sequence="GGAAACAGCTATGACCATG"
                     /Hairpin_Tm="None"
                     /Tm="53.8"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="M13_reverse_primer"
     primer_bind     complement(9723..9745)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Hairpin_Tm="None"
                     /Tm="59.7"
                     /Self_Dimer_Tm="None"
                     /%GC="43.5"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="M13_pUC_rev_primer"
     promoter        complement(9759..9788)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="lac_promoter"
     rep_origin      complement(10112..10700)
                     /note="pMB1 origin of replication (clockwise) (RNAII -35
                     to RNA/DNA switch point); pMB1 origin of replication
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point);
                     pUC19 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point); pUC19 origin of replication
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point)"
                     /Transferred_From="LITMUS 28i, LITMUS 38i, pGPS3, pNEB193,
                     pNEB205A, pNEB206A, pNEBR-X1, pUC19"
                     /Transferred_Similarity="100.00%"
                     /label="rep origin"
     gene            complement(10871..11731)
                     /gene="Ampicillin"
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="Ampicillin"
     promoter        complement(11773..11801)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca acgagcactt ccggaatctc tcggcgtgtg
     1021 agcttggttg tcctaccaaa gccagcgttt cggctcgcgt gcgccggcct agtttgctcg
     1081 cgtcctcacg cgctttgggt ttcccggtct catggccggc ctgaccttat ttgtgggccg
     1141 cctcccgccc tcggcccgca gtgagcagct ggaggaactg ttcagtcagg tggggccggt
     1201 gaagcagtgc ttcgtggtga ctgaaaaagg taggggcggg gcggtcgagg atagggctgg
     1261 ggtttcctgc gatcgagggc accttcccaa acttcaggga gccgtatcat tctttaccct
     1321 ccccgaagag aagggacagg gaactgagtt tcttttccaa actgattctg ctttagatgc
     1381 gttcatagga gtcagtttat gaagggcaaa gcattccctt acgtttctat agcgctttct
     1441 gttgattatg cgcgctaaaa atgaaaatag ttaactagag catcagtaag acttattgag
     1501 agtcacctga ttggcaggta cagtgatcag tttgatacag gttatctcat ttaattttct
     1561 cagcaatctt accggatgga gagtcttaat gtcattttac agacaggaat ccgggattta
     1621 ggggaattta gtaactttaa gtttgtacgg ctgttaagta gtggaccccc gatttgatcc
     1681 tcagacctgg acatcttagc caggatgcta tccacttgtt cattcattta ttcagcaaat
     1741 acttaatacc tactgtgtgc caggaagtgt tgtaggtcct ggggatgcag cagtgcacaa
     1801 caccaaacca aaaaaatctt tgattttatg tcttcaaggt ggggaagaga gccaataaac
     1861 gagccaataa acaagccaac cgtgaaaatg atgtcaaatg ctaaggagaa aaataagcca
     1921 gggaaagggg ttaggaagtc tatgtgcatg tgtatagtgg ggagaggggg tgaagagtgg
     1981 caatatagat agggtggcca gagaaggacg gactgaggtg agagagtgaa cacatgcctc
     2041 tccggaggaa gaacaattca ggaagagaaa acagcaagct caaaggtact gaaacagagg
     2101 tgtttttggt atattggagg agtagtaagg aggacagggt gactggattg aagtgagtaa
     2161 agggaagaga attaggagat gagatctaga gtagcagatc atgtaagatt tttttttttt
     2221 tttaaagact gcgtcttgct ctgtcgccca ggcgggagtg cagtgccacg atctccgctc
     2281 actgcaacct ctgcctcctg gttcaaccca ttctcctgcc tcagcctccc aagtagctgg
     2341 gattacaggc gagtgtcact acacctggct aatttttgta tttttagtag agacggggtt
     2401 tcgctatgtt ggccagggtt gtctggaact cgagacctca ggtgatccgc ccgcctcatg
     2461 taggatgtta tagaccattg caaggacttt ggcttttact cagagatagg aagccactga
     2521 ttttaagcag agagcagtaa gtgtgatctg gcattcgttt tgaaagattc tctctctggc
     2581 tggtgtgttg ccaatagact gttggagaat aggacaaaag cttatgtact taaaataggg
     2641 accgaaagtc ctgtacagta atgtttatta aactgcagat tattacctat tagtgggtct
     2701 tgagtgggtc atgactagag tttgttttgt tgtgttttag aaacagggtc ttgctctgtc
     2761 aaccctgttg acagttggag tgcagtggtg cgatcacagc tcaatgtaac cttaaactcc
     2821 tgggctcaag caatcctcct acttcagcct cccaagtagc tatgactaca gacgtgcttc
     2881 accatgcctg gccaattttt gaatattttt tgtagagaca gggtctcact acgttgtcca
     2941 ggctggtttc aaatgatcct cctgccttgg cctcccaaag cactgggatt acaggcatgt
     3001 gccaccacgc ctggcctaga gttcttaaaa aattaaatag tataggagta cgtccatata
     3061 tggtaaagga taagtatttg taaaactttt aagttttaaa taaagaatat atgtatgtac
     3121 tgggttccag tgtaagtagc attccgtacc caggtggttg tattccaggg aagaataggc
     3181 cattgaatgg gagttctcct ttgagtcctc agaatatagg ctacatgcaa tcttacctta
     3241 caccttgtgt acttggtcta aatccttaaa aatctcttac ttccttttaa aaaactatgc
     3301 actttcctat ccagacagct gatatgtact ggaaagagtg ctgcactcag tgaaacggga
     3361 gggagggtat gtggaatgaa gtcttcctca atagcagtgc atgtcacttc acctctttaa
     3421 gatgggaata atttcacagg gttgtcaggg actctaaatg tatgtgagca tgctttatgc
     3481 agacatgtaa aatgtgtggt atttactgta tgtcctttca tttgggtgtc aaactctttt
     3541 acatttcagc tgctttactt ttggttgtac tgatcatgaa gggctatgaa ttttcttgtg
     3601 ctcattttct gaaaggtgag tgggcagctt ttatgataag gagttcaaga gaagagaaaa
     3661 attgatgtta tgttgcttta accagtttat gtagatcagt gtcctgtgtc aaagggctta
     3721 taccctgaag gagattcaaa tgcagtggaa agagaagcag agcatatgaa ctatgtgtcc
     3781 ttttcaggta aaggcagact tcttgatctg tccttttcct ttttgatgca ataaattatt
     3841 taaaatttta ctatacaaaa tgttaaacca aaaagggcaa aggtgggtta cagttgatat
     3901 ttgtagtttt catctccttg gtcctaatat tattctttgt ggagtcctat ggaagaacat
     3961 gggggctggt gaggaggagt taattcagct actaacaccc tgtgtgacat taaccaattc
     4021 acttactgtc ttccatctca aattacttac ttggaaagaa ggggtgataa tatttgcttt
     4081 acctattttg aggtgttcta aagataaaat gagacaagaa tgcacagctg ctttggaaag
     4141 gtttaatttg atatcgacag ctaaggcgtt ggtaatagtg gtcataacat ctgcagtctc
     4201 tctgagcagt atttattgtt tgaggttctt ttgtctgggc ctcaggcatt tattgctgat
     4261 ctattttgag gagtaactgc attattgtgt tctgattttt gaagctacca tactgtctta
     4321 attcattttc tgttgcttat aagagaatac ctgaaactag gtactttgta aagaaaaatt
     4381 tatttcttaa gttctggagg ctgagaagtc caaagtttag tggctgtatc tggtgagagc
     4441 cttcttgttg gtggggactc tgcaaaatgc ccagacagtg cagggtgtca tggcgagggg
     4501 gctgagcatg ctaatgtgct tgctcaggtc tctcttcctc ttcttataaa gcccccagct
     4561 ccactctcat gatagcccat taatctatta acccattaat ccataaattg attaatccat
     4621 tcctgaagac agagtcctct gatccagtca cctcttaaag actccacctt tcaatactgc
     4681 tacattgggg attaagtttc caacacatga aatttggggg acacattcaa accatagcat
     4741 atacttacct ttcctgcctt ctttggtgat gttttgttct ttttgatgtt gagttctttg
     4801 agggcctcat atgtcatgga ttctctgttt tcttgggatt atcaagaaag ggggaagaag
     4861 gggagctaac agcaaagaca ctaaattaaa agaggaggaa ggctaacagt ttctaagcaa
     4921 agtggggagg gaaccactga taccaccctt gtttctcgcc ctcctgagac ttttggcaaa
     4981 ctgatagcaa gatgggttgg cagtatatcc tctagcttag gctttgagga tttactcatt
     5041 tagtgctctt ggcttatgat tctctctcct tttcacactg cccctccact ccctccccac
     5101 tccattattc cagggagtaa ggcatgtcga ggctttggct atgtcacttt ttcaatgctg
     5161 gaagatgttc agagggccct caaggagatt accacctttg aaggttgcaa gatcaacgtg
     5221 actgttgcca agaaaaaact gaggaacaag acaaaggaaa aggggaaaaa tggtgagttt
     5281 ctagtaattg aaggaagttt ttctcccagg gtgaggagga gcactatgaa aatgaaagag
     5341 ggaggcatgg aggtagcttc tagaacttaa agctttttgc atttcttttt gatctccaaa
     5401 atcatccagt gtattgtgat actcatttta gttctttgga aaaatctggt attccttggt
     5461 attattgcca cacttaaccc tgtggtaaat gccatctgtc gccagtagct tttttttctg
     5521 tgatgttctg gcgtagaagt gggtgatttt cgagtactta ttccattacc tcttattttt
     5581 tagaaaactc agagtgccca aagaaggagc cgaaggctaa aaaagccaaa gtggcagata
     5641 agaaagccag attaattatt cggaacctga gctttaaggt aagtgagaga gaaaatgaac
     5701 attgcatgtt ttgaaggctt ttagagccaa gatgtgatca ttagtagaac tcctcaccaa
     5761 gatggtataa ttgggcttaa ttccttctgg gaatcaacct tacccctgtt gaattcagaa
     5821 agtgggcctg tgtaataagg gcactaagtg tgaagctgct ttcctttttg gggaaactac
     5881 tactgagaaa gaaaaggtag aaaaagttct cagacgtctg tgtttttgcc tcttaggtac
     5941 aaagtaaaat gtcttctatt tagaaacatt tgatgtccta gtatcaaaaa taaaaaaaag
     6001 taaacattca tgtttttcat aattaatgaa tttagtagtt gatgttttat gttaatttgc
     6061 tgtgattgtg tttctctttt tctttcttca cttttggctt cagtgttcag aagatgactt
     6121 gaagacagta tttgctcaat ttggagctgt cctggaagta aatatcccta ggaaaccagg
     6181 tactaataaa ccttcacttg gtgtggatta cgttagttaa catattaccc ttgaacaaac
     6241 atatatgata tatatgaagt atttggcaca gtgcttcaca tctaatgaaa acccaaataa
     6301 tggttttatt cccctgatga tattttctgt tggatcagaa ttagttttag aaggcgagtt
     6361 agtttgagaa agtgttttgc tctccccaag tccaagtcag agtatactca gtaagaagat
     6421 gtaagcaatt gctttgggga agggcctgac ctgctggcag aatttgggcc atcttgtggg
     6481 cctgaattgc agtaatttac ttcttagtat tctggctacc tcctgctgcc tctcacttct
     6541 ttctttgtgc atttgcacac agatgggaag atgcgcggtt ttggttttgt tcagttcaaa
     6601 aacctcctag aagcaggtaa agctctcaaa ggcatgaaca tgaaagagat aaaaggtaag
     6661 ctttctatac ccatatcatt gacccagata tttctggtgt tttggtgaat tagtgtagta
     6721 acccactttc tgcattttca aaagatgttt gcattattaa gagtctcatt gctaatacgg
     6781 tagctattta ggtgaaatac tctagaactt gatcttgggg ttaaacttga aagaaccctt
     6841 gccagtattc tttcttttat ctggtgagag tgtcctttta aatccctagt gaaaaatgtc
     6901 cctgagataa ttgctgatag atgcaggttg gtatctagat tcctccttgg ttatatcact
     6961 gttcagcatc tgtgggctat ttattaaact gactgtgatt tatatattga cttatcaagt
     7021 actgtgccta aagaagttat tgccggccag gtgcggtggc tcacacctgt aatcctagca
     7081 ctttgggagg ccgaggaggc aggtggatca cctgaggtca ggagttcaag accagcctgg
     7141 tcaacatggc gaaaccccgt ctctactgaa aatacaaaaa ttagccaggt gtggtggtgc
     7201 atgcttgtaa tcccagctac tcaagaggct gaggcaggag aatcgcttga acctggaggc
     7261 ggaggttgca gtgaactgag atggcaccac tgcactccag cctgggcgac agagcgagac
     7321 tgtgtctcaa aaaaaaaaaa aagaagttat tgccgtagag atgtcctagc ttccaggtag
     7381 ggtgcagatg attccagaat gcctgttttg attcctgata gtctaactgg aaacatagtt
     7441 ctaattcttt atttttttac tccatcttat cccaggtgtg gtggctatgt agaaaggcaa
     7501 aatttctgtt cattgataaa aaggttaagg gttaaaagga gtaggcagtg actaggagct
     7561 ttattttaag accagcattc cctgaattgg cagtttcttt gtctccactg gttccattat
     7621 tgtctgtgat gactaggggt catttacaga ggtagggttt ttaaaaggag agcctttctg
     7681 atgccacttt tctgcttgac aggccggaca gtggctgtgg attgggccgt ggcaaaggat
     7741 aaatataaag atacacagtc tgtttctgct ataggcggcc gctcgagtct agagggcccg
     7801 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7861 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7921 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7981 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     8041 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     8101 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8161 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8221 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8281 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc
     8341 tattccgaag ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac
     8401 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     8461 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     8521 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     8581 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     8641 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     8701 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     8761 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     8821 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     8881 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     8941 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     9001 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     9061 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     9121 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     9181 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     9241 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     9301 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     9361 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac
     9421 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     9481 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     9541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9661 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     9721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
