LOCUS       Exported                7929 bp ds-DNA     circular SYN 15-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Universitaetsklinikum Halle
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 25, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    401..428
                     /label=over_rev''
     misc_feature    430..811
                     /label=CMV IE enhancer
     misc_feature    826..1058
                     /label=SV40 enhancer
     misc_feature    1069..1079
                     /label='over_rev
     misc_feature    1075..1487
                     /label=RSV/5'LTR
                     /note="LTR"
     misc_feature    1538..1675
                     /label=psi
     misc_feature    1642..1996
                     /label=gag'
     misc_feature    1997..2006
                     /label='gag
     misc_feature    2152..2393
                     /label=RRE
     misc_feature    2826..2943
                     /label=cPPT
     misc_feature    2992..3234
                     /label=EFS
     misc_feature    3247..3966
                     /label=eGFP
     primer_bind     3946..3983
                     /label=IF_mArid3a_UTR_Fw
     misc_feature    3974..4176
                     /label=mArid3a_WT
     misc_feature    3974..4004
                     /label=gBlock_1
     misc_feature    3985..3991
                     /label=let-7 binding
     misc_feature    3985..3991
                     /label=let-7 binding site
     misc_feature    4005..4040
                     /label=gBlock_2
     misc_feature    4019..4025
                     /label=miR-125b binding
     misc_feature    4019..4025
                     /label=miR-125 binding
     misc_feature    4019..4024
                     /label=miR-125b binding
     misc_feature    4041..4071
                     /label=gBlock_3
     misc_feature    4051..4058
                     /label=miR-125b binding
     misc_feature    4051..4057
                     /label=miR-125b binding
     misc_feature    4052..4058
                     /label=miR-125 binding
     misc_feature    4072..4101
                     /label=gBlock_4
     misc_feature    4084..4091
                     /label=let-7c binding
     misc_feature    4084..4090
                     /label=let-7 binding
     misc_feature    4085..4091
                     /label=let-7 binding
     misc_feature    4085..4091
                     /label=let-7 binding site
     misc_feature    4102..4151
                     /label=gBlock_5
     misc_feature    4109..4115
                     /label=let-7 binding
     misc_feature    4109..4115
                     /label=let-7 binding site
     misc_feature    4112..4118
                     /label=miR-125b binding
     misc_feature    4140..4146
                     /label=miR-125b binding
     misc_feature    4152..4176
                     /label=gBlock_6
     misc_feature    4161..4167
                     /label=let-7 binding
     primer_bind     complement(4164..4197)
                     /label=IF_mArid3a_UTR.WT_Rev
     misc_feature    4177..4180
                     /label=eGFP
     misc_feature    4197..4785
                     /label=WPRE
                     /note="misc_RNA"
     misc_feature    4856..5091
                     /label=3'\LTR\SIN
                     /note="LTR"
     misc_feature    5168..5298
                     /label=SV40\polyA
                     /note="polyA_signal"
     misc_feature    5645..6075
                     /label=f1\Ori
                     /note="rep_origin"
     misc_feature    6446..7252
                     /label=AmpR
     misc_feature    complement(join(7380..7929,1..64))
                     /label=pUC\Ori
                     /note="rep_origin"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gttggctccg
     3001 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     3061 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     3121 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     3181 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtgtcg tgacggatcc
     3241 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3301 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3361 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3421 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3481 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3541 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3601 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3661 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3721 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3781 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3841 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3901 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3961 aagtaaacat gtcgcacgtc tgtttacctc atgaatcccg gcacatattt gtgttcattc
     4021 agggaaacag acttttttta gggtaactgg ctcagggata tagcagccag cgcaagtttc
     4081 tttctacctc aggtcttttg acacctggta cctcagggct tggcgttgag acacccaaac
     4141 tcagggcaaa gaactgtttt ctacctctgg tcaggcgtaa aacgcgttaa gtcgacaatc
     4201 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4261 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4321 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4381 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4441 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4501 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4561 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4621 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4681 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4741 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     4801 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4861 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4921 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4981 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5041 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     5101 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5161 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5281 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5401 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5461 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     5521 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5581 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5641 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     5701 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5761 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5821 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5881 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6001 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6061 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6121 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6481 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6781 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7921 gccttttgc
//
