LOCUS       Exported               10161 bp ds-DNA     circular SYN 06-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Universitaetsklinikum Halle
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 25, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        271..301
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    401..428
                     /label=over_rev''
     misc_feature    430..811
                     /label=CMV IE enhancer
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    826..1058
                     /label=SV40 enhancer
     misc_feature    1069..1079
                     /label='over_rev
     misc_feature    1075..1487
                     /label=RSV/5'LTR
                     /note="LTR"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1538..1675
                     /label=psi
     misc_feature    1642..1996
                     /label=gag'
     misc_feature    1997..2006
                     /label='gag
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2152..2393
                     /label=RRE
     misc_feature    2826..2943
                     /label=cPPT
     promoter        3001..3407
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     3442..3471
                     /label=IF Luc.OA Fw
     CDS             3448..5097
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     misc_feature    3961..3967
                     /label=let-7 binding
     primer_bind     complement(5083..5122)
                     /label=IF_Luc.OA Rev
     primer_bind     5103..5132
                     /label=IF IRES-dTomato Fw1
     misc_feature    5116..5702
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5681..5718)
                     /label=IF IRES-dTomato Rev1
     primer_bind     5693..5727
                     /label=IF IRES-dTomato Fw2
     CDS             5709..6403
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDE"
     primer_bind     complement(6391..6413)
                     /label=IF IRES-dTomato REv2
     CDS             6404..6413
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="YK"
     misc_feature    6405..6410
     misc_feature    6429..7017
                     /label=WPRE
                     /note="misc_RNA"
     CDS             complement(6900..6911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    7088..7323
                     /label=3'\LTR\SIN
                     /note="LTR"
     LTR             7089..7322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7400..7530
                     /label=SV40\polyA
                     /note="polyA_signal"
     polyA_signal    7400..7521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7561..7696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7717..7735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7745..7761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7877..8307
                     /label=f1\Ori
                     /note="rep_origin"
     rep_origin      7903..8358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8384..8488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8489..9349
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    8678..9484
                     /label=AmpR
     rep_origin      9520..10108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(join(9612..10161,1..64))
                     /label=pUC\Ori
                     /note="rep_origin"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gctagctgca
     3001 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
     3061 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
     3121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
     3181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
     3241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
     3301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
     3361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cggccggtcg aatcaagctt
     3421 atcgataccg tcgacggatc caccggtatg gaagacgcca aaaacataaa gaaaggcccg
     3481 gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag
     3541 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc
     3601 acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     3661 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     3721 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     3781 cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag
     3841 gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc
     3901 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     3961 ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca
     4021 attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg
     4081 catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc
     4141 gttccggata ctgcgatttt aagtgttgtc ccattccatc acggttttgg aatgtttact
     4201 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     4261 ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta
     4321 ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     4381 attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc
     4441 catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt
     4501 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     4561 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt
     4621 gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     4681 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     4741 ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca ggtggccccc
     4801 gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt
     4861 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     4921 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     4981 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     5041 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgctc
     5101 gagtgaacgc gttcgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     5161 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5221 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5281 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5341 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5401 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5461 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5521 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5581 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5641 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     5701 ccggatccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     5761 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     5821 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     5881 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     5941 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6001 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     6061 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     6121 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     6181 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     6241 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     6301 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     6361 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag tgatcgcgtt
     6421 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6481 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6541 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6601 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6661 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6721 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6781 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6841 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6901 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6961 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     7021 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7081 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7141 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7201 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7261 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7321 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7381 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7441 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7501 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7561 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7621 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7681 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     7741 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7861 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     7921 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7981 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8041 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8101 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8161 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8221 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8281 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8341 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9541 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9601 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9661 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9721 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9781 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9841 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9901 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9961 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10021 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10081 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10141 ggccttttgc tggccttttg c
//
