LOCUS       Exported                4606 bp ds-DNA     circular SYN 27-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   ML
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Mar 27, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             773..1567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1585..2445
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2616..3204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3679..4252
                     /label=U6-6 (CPIJ039596) Promoter
     misc_feature    4253..4270
                     /label=2xBbsI spacer
     misc_feature    4271..4346
                     /label=gRNA scaffold
     misc_feature    4347..4355
                     /label=Ts
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
      481 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
      541 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
      601 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
      661 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
      721 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
      781 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
      841 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
      901 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
      961 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     1021 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     1081 gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     1141 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     1201 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     1261 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     1321 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     1381 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     1441 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     1501 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     1561 cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1621 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1681 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1741 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1801 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1861 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1921 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1981 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2041 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2101 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2161 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2221 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2281 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2341 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2401 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3601 tatgaccatg attacgccaa gcttggtacc gagctcggat ccactagtaa cggccgccag
     3661 tgtgctggaa ttcgcccttc aaaatctacg aagataataa ggtctactat tacatttaat
     3721 aaatattctt atttaatttg agctgtgtaa aattagtgcg tttatccggg gaatgtttat
     3781 attttatccg aggatttccc gaaattgatt aaaaaaatca aatctcttaa aaaattagaa
     3841 acaacctgtt aaaaataagc ttatagaaaa aatgtaaagt gttgctacaa ggacaatcaa
     3901 ttaatcatca taacgaaaac ttttaggatc ttgaaaagca cgtcccgaga tgggaaagtg
     3961 tgtttcattt ccaggcgatc cttttcgctt cacgaaattt ttgattgcta cctcgtttag
     4021 agccctaaga caaaatactt cttcaaaaaa aagcgaactc gaacccaaac tttgtactac
     4081 tatgctaaaa tgtctgcatg catgggatgt cgcgcaaacg agtccaataa gaaataaaac
     4141 tgctcgaatt caacgtgacg ctacccgcaa tgctctaccc ttaactttat acatcgctag
     4201 aaccgcgttc gcattcgcta actatataag cactttcccg gccctacaac ttgggtcttc
     4261 gagaagacct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4321 ttgaaaaagt ggcaccgagt cggtgctttt tttttagggc gaattctgca gatatccatc
     4381 acactggcgg ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta
     4441 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4501 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4561 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgg
//
