LOCUS       Exported               16134 bp ds-DNA     circular SYN 28-MAR-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16134)
  AUTHORS   Valentino
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 28, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16134
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
     CDS             773..1567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1585..2445
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2616..3204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3680..4379)
                     /label=Actin5C (CPIJ009808) 3' Sequence
     CDS             complement(4383..4403)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large 
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4410..8513)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the 
                     Streptococcuspyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    complement(8514..15882)
                     /label=Actin5C (CPIJ009808) Promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
      481 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
      541 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
      601 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
      661 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
      721 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
      781 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
      841 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
      901 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
      961 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     1021 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     1081 gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     1141 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     1201 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     1261 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     1321 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     1381 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     1441 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     1501 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     1561 cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1621 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1681 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1741 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1801 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1861 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1921 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1981 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2041 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2101 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2161 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2221 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2281 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2341 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2401 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3601 tatgaccatg attacgccaa gcttggtacc gagctcggat ccactagtaa cggccgccag
     3661 tgtgctggaa ttcgcccttc tatttgatga acgacgcact ctcggaataa attaattgct
     3721 aataaatatt ttgacaagcc taaacacaaa acgatacaaa acggtcattc gatttgggca
     3781 gttggttttt ttcgcttcta gcttcttttt taaacttttt tttcgtgatt ttcttatttt
     3841 ttctgacttc gcgctcctac aaaattacaa aaaagaaaaa tgaaagtatc gctaacttga
     3901 gtaactgtct gtcttttttt tttttggttc tctcactcgc gctcgctcgc acactcgaag
     3961 tttaacgcgc tcgctcgcta tctcaacatg gaataatatg ataattgcgt atgtgtgtgt
     4021 gtttttgtgt gggtgactaa ataattcaca aaatcaaatc attaaataaa cgtgtggtac
     4081 ttcgatatta taagtaataa tacaggaatt gtctttttat ttttaagttg ttctcaaatg
     4141 tgtgtgttgt ttgtgtgtat gtgttttttg agtcattcaa cacttcggat gttcttgctg
     4201 ggaatgattt ttgagatgat gttgcgctgt tgttgttgct gttgattttt cctcgctttg
     4261 atggaaagta tctggttttt tgtttgtttt tttaattata aaggagaagg acgacgatca
     4321 cgcggccgcg atcggggtcc tgtgcagggg atggtgttgt tgcggcgtga aggcgcttat
     4381 tacaccttgc gcttcttctt cggcgaacca tcaccaccca gctgcgacag atcgatgcgc
     4441 gtctcgtaca gacccgtgat cgactggtgg atcagcgtgg catccagcac ctccttcgtc
     4501 gacgtgtagc gcttgcgatc gatcgtcgta tcgaagtact tgaaggcggc cggggcaccc
     4561 aggttcgtca gcgtgaacag gtggatgatg ttctcggcct gctcgcggat cggcttatcg
     4621 cggtgcttgt tgtaggccga cagcacctta tccaggttgg catcggccag gatcacgcgc
     4681 ttcgagaact ccgagatctg ctcgatgatc tcatccaggt agtgcttgtg ctgctccacg
     4741 aacagctgct tctgctcgtt atcctccggc gaacccttca gcttctcgta gtgcgaggcc
     4801 aggtacagga agttcacgta cttcgacggc agggccagct cgttaccctt ctgcagctca
     4861 ccggccgagg ccagcatgcg cttgcgaccg ttctccagct cgaacagcga gtacttcggc
     4921 agcttgatga tcagatcctt cttcacctcc ttgtaaccct tggcctccag gaaatcgatc
     4981 gggttcttct cgaacgacga gcgctccatg atcgtgatac ccagcagctc cttcaccgac
     5041 ttcagcttct tcgacttacc cttctccacc ttggccacca ccagcaccga gtaggccacc
     5101 gtcggcgaat cgaaaccacc gtacttcttc ggatcccaat ccttcttgcg ggcgatcagc
     5161 ttatccgagt tgcgcttcgg caggatcgac tccttcgaga aaccacccgt ctgcacctcc
     5221 gtcttcttca cgatgttcac ctgcggcatc gacagcacct tgcgcaccgt ggcgaaatcg
     5281 cgacccttat cccacacgat ctcacccgtc tcaccgttcg tctcgatcag cgggcgcttg
     5341 cggatctcac cgttggccag cgtgatctcc gtcttgaaga agttcatgat gttcgagtag
     5401 aagaagtact tggccgtggc cttaccgatc tcctgctccg acttggcgat catcttgcgc
     5461 acatcgtaca ccttgtaatc accgtacacg aactccgact ccagcttcgg gtacttcttg
     5521 atcagggccg tacccaccac ggcgttcagg taggcatcgt gggcgtggtg gtagttgttg
     5581 atctcgcgca ccttgtagaa ctggaaatcc ttgcggaaat ccgacaccag cttcgacttc
     5641 agcgtgatca ccttcacctc gcggatcagc ttatcgttct catcgtactt cgtgttcatg
     5701 cgcgaatcca ggatctgggc cacgtgcttc gtgatctggc gcgtctccac cagctggcgc
     5761 ttgatgaaac cggccttatc cagctccgac agaccaccgc gctcggcctt cgtcaggtta
     5821 tcgaacttgc gctgcgtgat cagcttggcg ttcagcagct ggcgccagta gttcttcatc
     5881 ttcttcacca cctcctccga cggcacgtta tccgacttac cgcggttctt atccgagcgc
     5941 gtcagcacct tgttatcgat cgaatcatcc ttcaggaacg actgcggcac gatgtgatcc
     6001 acatcgtaat ccgacaggcg gttgatatcc agctcctgat ccacgtacat atcgcgaccg
     6061 ttctgcaggt agtacaggta cagcttctcg ttctgcagct gcgtgttctc caccgggtgc
     6121 tccttcagga tctgcgaacc cagctccttg ataccctcct cgatgcgctt catgcgctcg
     6181 cgcgagttct tctgaccctt ctgcgtcgtc tggttctcgc gggccatctc gatcacgatg
     6241 ttctccggct tgtggcgacc catcaccttc accagctcat ccaccacctt caccgtctgc
     6301 aggataccct tcttgatggc cggcgaaccg gccaggttgg cgatgtgctc gtgcagcgaa
     6361 tcaccctgac ccgacacctg ggccttctgg atatcctcct tgaacgtcag cgaatcatcg
     6421 tggatcagct gcatgaagtt gcggttggcg aaaccatccg acttcaggaa atccaggatc
     6481 gtcttacccg actgcttatc gcggataccg ttgatcagct tgcgcgacag gcgaccccaa
     6541 cccgtgtagc ggcggcgctt cagctgcttc atcaccttat catcgaacag gtgggcgtac
     6601 gtcttcaggc gctcctcgat catctcgcga tcctcgaaca gcgtcagcgt cagcacgata
     6661 tcctccagga tatcctcgtt ctcctcgtta tccaggaaat ccttatcctt gatgatcttc
     6721 agcagatcgt ggtacgtacc cagcgaggcg ttgaagcgat cctccacacc cgagatctcc
     6781 accgaatcga agcactcgat cttcttgaag taatcctcct tcagctgctt caccgtcacc
     6841 ttgcggttcg tcttgaacag cagatccacg atggccttct tctgctcacc cgacaggaag
     6901 gccggcttgc gcataccctc cgtcacgtac ttcaccttcg tcagctcgtt gtacaccgtg
     6961 aagtactcgt acagcagcga gtgcttcggc agcaccttct cgttcggcag gttcttatcg
     7021 aagttcgtca tgcgctcgat gaacgactgg gccgaggcac ccttatccac cacctcctcg
     7081 aagttccacg gcgtgatcgt ctcctccgac ttgcgcgtca tccaggcgaa gcgcgagtta
     7141 ccgcgggcca gcggacccac gtagtacggg atgcggaacg tcaggatctt ctcgatcttc
     7201 tcgcggttat ccttcaggaa cgggtagaaa tcctcctggc ggcgcaggat ggcgtgcagc
     7261 tcacccaggt ggatctggtg cgggatcgaa ccgttatcga acgtgcgctg cttgcgcagc
     7321 agatcctcgc ggttcagctt caccagcagc tcctccgtac catccatctt ctccaggatc
     7381 ggcttgatga acttgtagaa ctcctcctgc gaggcaccac catcgatgta accggcgtaa
     7441 ccgttcttcg actgatcgaa gaagatctcc ttgtacttct ccggcagctg ctggcgcacc
     7501 agggccttca gcagcgtcag atcctggtgg tgctcatcgt agcgcttgat catcgaggcc
     7561 gacagcgggg ccttcgtgat ctccgtgttc acgcgcagga tatccgacag caggatggca
     7621 tccgacaggt tcttggcggc caggaacaga tcggcgtact gatcaccgat ctgggccagc
     7681 aggttatcca gatcatcatc gtacgtatcc ttcgacagct gcagcttggc atcctcggcc
     7741 agatcgaagt tcgacttgaa gttcggcgtc agacccagcg acagggcgat caggttaccg
     7801 aacagaccgt tcttcttctc acccggcagc tgggcgatca ggttctccag gcggcgcgac
     7861 ttcgacaggc gggccgacag gatggccttg gcatccacac ccgaggcgtt gatcgggttc
     7921 tcctcgaaca gctggttgta cgtctgcacc agctggatga acagcttatc cacatccgag
     7981 ttatccgggt tcagatcacc ctcgatcagg aagtgaccgc ggaacttgat catgtgggcc
     8041 agggccaggt agatcaggcg cagatcggcc ttatccgtcg aatccaccag cttcttgcgc
     8101 aggtggtaga tcgtcgggta cttctcgtgg taggccacct catccacgat gttaccgaag
     8161 atcgggtggc gctcgtgctt cttatcctcc tccaccagga acgactcctc caggcggtgg
     8221 aagaacgaat catccacctt ggccatctcg ttcgagaaga tctcctgcag gtagcagatg
     8281 cggttcttgc ggcgcgtgta gcggcggcgg gccgtgcgct tcaggcgcgt ggcctcggcc
     8341 gtctcacccg aatcgaacag cagggcaccg atcaggttct tcttgatcga gtggcgatcc
     8401 gtgttaccca gcaccttgaa cttcttcgac ggcaccttgt actcatccgt gatcacggcc
     8461 caacccaccg agttcgtacc gatatccaga ccgatcgagt acttcttatc catgttgata
     8521 tctgcgcgca agaaggggag aagattcaga ttagattgcc agattcaacg gtacaaagct
     8581 tcgcgagaga gagagattgc aacaaccgtt gggattaact gttatctaca cgcgcgccac
     8641 ggacaagata atgcgaggcc acgtcagcgg aaagtaccac atgaccaacc caacgcagtt
     8701 aattaaatta cgatcgaatt acttgcgttg aaatctctcc acaaactcgg tatcgtgcgc
     8761 tctctccgtc tctcagttgg ttcgtgtctt gcgcgctttc tcccttcctc cgtctgcggg
     8821 gttctgcagg tagcgaggac cacgcagaca acgcccacgc agaaaaaggg accgtcttcc
     8881 cgggaaggga gaggggagaa agagggcaag acaagcgagc tgaaaattgt tcgactgtgc
     8941 aggcagggaa cctgtttcta cggcgggggt ccgtttcttg agtgtttctt gacgcgggta
     9001 gacgactttg cgggggattt ggaaccgggt agagtcggca acggcggggg tgtgttaatt
     9061 gattttgagg aaagagactt gagtgagcaa ttagcggcag acggtgacgc tgggaggtaa
     9121 aaatttgacg atttaggctg atttgcacga aaatgaggtg atgaaatcgg ctttgaaaaa
     9181 tcatcaaatg gataaatttt aatgtttttc ctaaaacatt gcagaaatac attgattgaa
     9241 acgttgttct acgtcaatta ttgaacattt acagtcaatt tgatgattcc tgcttgacaa
     9301 caaatgttaa tgaaagatta agtttaaact tgaaacatta aaatattctt aaattgtcaa
     9361 gcgtagtccc acgtcaatca aataaataat taattcccta tacaacatgt caaatttgaa
     9421 gcaagtttga caagtttttt tcaaaatccg ggaaattttt atttaattaa aaaaaaaaac
     9481 aaaattaaat ctacaaattt caaaaagttg ataaaattaa gcaaaatttt accccacttt
     9541 cccctttaaa aaatctcacc aaaacccaag ccgcttgact caactgtcaa gagaggggtg
     9601 caacaacttt ttttcatctc cgttttattc caaatttccc agaagagcga tcaagaacct
     9661 aggctcttct tcttttctct tcccctcaca cattcacagc agcataattg gatacaaacg
     9721 ccgcttgctg tgtgagatga cgtggggtgg gctgcacttt ggtgcgcgcc ctcctctgca
     9781 gttgaatctt ccttttcgct ctttggctgt gtgagtgaga aaaggatcac actttttctc
     9841 cttctttttt ttcaagttgg gagaagtcaa cctttttggg cttgtctagc cacttaaaat
     9901 atgtcaaaat ttaaaatttt taaaatttaa atttccaaca atcttcaaaa atgttgaagt
     9961 ttaaaaagga tttgccagaa tcgcaataaa aatcgattcg agccggcatt ttggcacgtc
    10021 cccacgcaca cactctcaca ctgcagacct ccggccggaa caaaatgccg gccaacgccc
    10081 cacacaccgg ccacaacccc tcttaccctt ttcttacaaa agtgagaaaa atgaccgtta
    10141 cacaggttct acttcaactt caaaaaaact acacgaaatt ttccagaaaa tttaaaaaaa
    10201 cgcaccgttt gaaaattctt ttgacagcag ctaacaaaag aaagggaacc accacacttg
    10261 gattgctttg atttgagtga ctttagaata atttgcaccg gcacacacac gcgaccgcac
    10321 cagccgaacg acaacgagcg aatgagaggt gggcttgggc ttcgggctcg gatggtggct
    10381 ctccttcgct aacggctctt ttcgccctct ctcattctcg cgagagcgcg caactttcga
    10441 aaagttctca cccgagccga atctgacgcg agagaaaacg attacgaact tttcaaaagc
    10501 ttccaccgga tctttctgga aggcacttcg cggcctggag aatgagagag cgggagcgag
    10561 ctaacccgct cgcgctctca cccttttacg gagcgtcgac caatcgcgtg aacatgacca
    10621 tataaggcaa tgtttgaaaa atgctcaatt tccagcggtc caagaaggcc ggttttcgca
    10681 cacgtgaact tgccggtaaa gatggtggtg ggaatgggag aggaagagag agctttataa
    10741 ggtgtgtgtg tgcgcgaact tgaaaagctt gcgggggttg cttggagagg ggaaggaaag
    10801 tcagccgacg cgtcatccgg tggggtggtt ccacggttga aattttgtga taaatttgag
    10861 tttaaaataa taattttata gaaacttttc aaaaatttct taaaatgttg cctgtcaaag
    10921 gaaacttaaa agagttagat ttttttactt gttcaaacat ttttttttgc aaattcaatt
    10981 gaaaaaaaat ttggaagatg ttcaattgaa aagcaatcaa aatcaaaatt tttgaagttt
    11041 cttgaacaga ttcttcaaca aacatacatc cagcaaaaca aacgtgcttc gaatgtcaaa
    11101 catcgctcga cacgtaggaa atgcaaaaaa tccaccaatt tatgtaactc aaaagaggtg
    11161 gctagacgtg acaatttccc catcaacctt caagaacttg ccaaacaaaa gaaagttgat
    11221 aaaatttggg cgcgctgcag gtagaaacac ttgcaaccaa gataaaaact cgttttgcag
    11281 gtaaaaatag caaaatttcg aggttcaaac agcccttggc gtagtttgaa gtgtgcggtg
    11341 cacaaacagg gctggaattg tttgtaaaca aatcgttaca tgcacggtgg aatgtcagag
    11401 ctggctttca aggtgatgac ggcggtttgg tgggcgcatc aagtaggcca cgtgtgctta
    11461 tcaggtggaa agtgggttac ttttcaatgt tgagtttgat agactttttg ttcttagtaa
    11521 aatttaagag ccagttttga caatagcaac ttgcctataa atggcattta ggatgaaaat
    11581 aaggcttgag atttatttat ttttgcgtgg gacaaacatt ttgatcaaaa tttgtacgag
    11641 cctaaaaaga ttttgaaaat taaattgatt atcgatttaa tgcactgatg tagacaaatt
    11701 tatgttgata tttgtcaaaa ttgaatctaa atctgcaaga ttttgtctga aatttatgtt
    11761 tgatttgata ttatttttag tttaatcata ttttcgccac ttttgttgat gaaatcttta
    11821 aaaacttcgt gagaattcat aaaaatgtcc atctgatcaa cattttccac acaatttcca
    11881 ccactttcca gcgcacatta gccacgttga cttagcctgc ttctcgcagc caaaagcggt
    11941 acatctccga gttgcattcc agcgctgtct cgccaccaaa ctttccccgc actgtctctc
    12001 atgattgacg tgggactaca ccccacgtcg ttattgttgc cacgtgtttc gagtggaacc
    12061 accacccaaa aaaatccgga cgagctgcat tccacccggc ttttgccgca cccaaaacca
    12121 aatcctgcgc gcggttcgtc gcttaatccg gaacccgttg aagtcacttt agagttcatt
    12181 gttttttcaa atgtcttttt gggcacaaat cactgcattt ttttgcgggg cacaacttgg
    12241 taaatgtcct ttgtagtcct tttttgagca cttgtcgaac aattttcact tgcttttttc
    12301 cgatgatttg tttttttcca tagacgacga aacttgttac ttacatttgg tttattaaat
    12361 acaaaaaagc ctaaattaca aataaattaa ttgtgtgctg tgagtttctg cgctggtgtt
    12421 tcgtagaaac gtaggctggt agctggtgct ttttcacaga aaataagtta ataaacagct
    12481 actactagtt tgttcgatct gttcgatctc tgtctctggt gtgttcacag cacgattaca
    12541 agaactgtag tgatacaatt tttaaacaac actcagatta taaatagtac ccctccaatc
    12601 cggacgggta tcccaacggt tgcctcggtt gattcggggt tcgctcgcgc ttatgaccat
    12661 atatggtatt ggcagtccgc cggaaccacc acagacgcgt tttttctcca gagaagaaac
    12721 tcacacactc tcacaaacag agagcgaacg aacgattcgg tgggcgctcg cagctagcga
    12781 accggcataa gcaggcggtg acgacgacga cggcgaggaa ccaacggaag tatcaagcgc
    12841 gatagagagg agaaaagaag cagccgagta ggctatgaag tgcccagggt tgggcacaca
    12901 cactcttctt cgttgaccga tgcaccatct cttcatgcat atgcatcgcc agagcagcgt
    12961 gtgtagaaaa gcatatcggc gtgtggggtg cggcgccacg ccgcacccag ccctcttgga
    13021 tcccactttt gacagtttgg ggttcaatca aatacttttt tcttcttaat tgtcagacgc
    13081 atctaatgac tttatggcac cctacttcac ttacttgttt catgttttgg cgttgccggt
    13141 tttccgatcg gagcaagtca tgcacaattt cgaggaatcg cttgaacgat ggtggcggcg
    13201 gttacgaata tccaacagca gagccaactt tgataacaaa gtgataagag gtaatcttgg
    13261 gggtgtcacg tcacctagtt tgagttggtt gttttttttc tttgcagaat tcttttgaaa
    13321 tagataacaa atggtcgcgt taaaggtgga accatcgatg gtattgtgta agaggtcccc
    13381 cccccctcgt gttatcagtt cgagtggtct ttgttctgaa ttatgtgaag aatgtcaacg
    13441 cgggaatgaa ggtcgtgaag gggatttttt tgcgcttgct gataaaaagt ttgatgacaa
    13501 atcaattaag tgtggagttt ttttaaggtc caataaacta tttttttact taaggcggag
    13561 aaaacaaata agaaaaattt ggtttattgg accttttaaa aaaagactct gaaagtgtgt
    13621 ttggactttt aaaaatgtta gtgttagttt tttgccaaat gaatttttaa tcatattttt
    13681 acagttttta aaaaaatatt ttattttaga aaaattaaaa aagagtcctt gaaaaacttg
    13741 tcgtttttat taaactaatt tcaccagtaa ctaaacgtac agacaatcag tctgtgctca
    13801 aagtttgcct aagacatcaa atcgatcaga aaccccgttc tcaagatccc gagtttttga
    13861 atatttaggt tgcatttttg tttggaaagc tggctgtttt gtttataaag gaagtactca
    13921 caatgttttg aattatgata aaattagggt acctccaaaa agttgtaaca attttatttt
    13981 tatgttttaa gcatgcacaa ttgtatactt gcggtctgtt tagtctgtgt ggaacggaca
    14041 ttttaaaatg gtcaaataca ggtccaagac caaatttggg ataaaaatgt cgttattaaa
    14101 tcatccaaag tgaaatatta gctaggactt tggataatga aaacgctttt ttcgcttgtt
    14161 ttttttgttt gcaaagttct gtgtatgtat taaaattgta ttattttaac tcacaacctt
    14221 ctgctctgga tgcagagggg atgaaaatta aaaacttgct gtaaaaatac aaacaaatta
    14281 aaaaagttcg agtttgaagt ctaatttaaa actgtgcctc ttttttacac cctgaccaaa
    14341 aatcctgatt ttctaagttt aatatctcac tttccatacg attttttttg agttcagatt
    14401 ctacaaccat aaaaaatggt tatatgagtt gaactgaaaa attcgtatga aaagaaggga
    14461 ataatgattt ttggtaaggg tgtacagagt aacctgattt gacaatttcg gctaaacaca
    14521 aaaaaaaaac acttgaaaat gttttatgaa gagttaaatt gtagtaattg tatttttttt
    14581 ttgatatttt tttttaagga tcgagacaat acatattttt ttttattttt tttgtccttc
    14641 tgctctggtt aagttcagaa gggatggaaa taaaaaactt actgccaaaa gtttacgaag
    14701 aaattttaaa ataacataaa atgtttgacg tctaatttca atctgtgctc caaatttttt
    14761 tagagtgtat cctgttttga caattttgac ttaaaacaac aaagtaaaac aagtttaatg
    14821 gcctatctac aatcactttg cttagaaaat ttcacttagc ttaggaattt gaatcaatgg
    14881 aaatgaatct gagtttctac aatggaaaag taatcaaatt agaaactgac gtttggtttg
    14941 gaaaatttca aaatctacgg taagttgacg tttccatatc aaaggtaaac aaacaactgc
    15001 atagcaacgt atatcagccc agcaagtttt ctaagttgat tgtggatgac gaacgtaagt
    15061 tgcagacttt cctaagtaac tactttacat agatgttcta agctaagtga ttgtagatag
    15121 gccataagtc caattttttg cgttacgtaa taaaagaacg ctccctttgt ggtcaaaagg
    15181 gggcatagag ttatctacgc gcgcatgtat tgcgtgtacg tacacgaaaa agattgaggt
    15241 taaattttgt atcggccagc aaaacaaatg gcgtgctagt gtgcgtgaga tcgggcttag
    15301 ataactctat tgagctgatc tgaataagct ttatcggtgt tgagatccag aaaaaagatc
    15361 cgacagcaat ttgtactgat ttgacgtttg ctgagggtgc caaaacaatt gcaaaagaca
    15421 aatattgctg tgaggatttg ttgattcgaa tttcgctact tcgaatattg gctatttcga
    15481 atgctcttca attcacattt gaatcaaaaa gcactcagtt gttaaaattg agttgtcaaa
    15541 ctgatgttta cattttgact cctttgtttg tcgtactttt gacagttgtt actcgttcag
    15601 atgagtgaat ctgctctgta tcattgttat ctacaaaact tttcaaaaaa caaagaatta
    15661 ttctaaaatc cccaaaaaaa tcccccaatg tttacactcc caagataagc ggaccttgac
    15721 atcaagatag catcttatca cccgctctcg agtgaatgtc aaaaaggcct cctccattaa
    15781 gtaaacaaac cgctgcggag cgggggaaaa atatttacac aaattactct cacagagtcc
    15841 accgcatccc agccaagccg ggggtaaatt ggcaaattgg ccaagggcga attctgcaga
    15901 tatccatcac actggcggcc gctcgagcat gcatctagag ggcccaattc gccctatagt
    15961 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    16021 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    16081 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgg
//
