LOCUS       Exported               13640 bp ds-DNA     circular SYN 27-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   xuechunfeng
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Mar 27, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             773..1567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1585..2445
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2616..3204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3680..7854
                     /label=nanos (CPIJ011551) Promoter
     CDS             7858..11958
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             11965..11985
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    11989..13388
                     /label=nanos (CPIJ011551) 3' sequence
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
      481 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
      541 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
      601 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
      661 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
      721 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
      781 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
      841 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
      901 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
      961 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     1021 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     1081 gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     1141 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     1201 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     1261 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     1321 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     1381 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     1441 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     1501 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     1561 cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1621 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1681 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1741 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1801 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1861 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1921 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1981 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2041 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2101 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2161 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2221 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2281 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2341 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2401 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3601 tatgaccatg attacgccaa gcttggtacc gagctcggat ccactagtaa cggccgccag
     3661 tgtgctggaa ttcgccctta tgtccttttt aatcacgatt cacagaaata aaaatttcac
     3721 accgttcacg caaggcactg cgactaatgt taaggcaaag ttagattttt taagggtcca
     3781 cccgttttgt ctggcaaggc aacaaatgtg aagagagact gacggctgca cggaaaggcc
     3841 gaccgacttt tatacgtccg ccgaacccga agtcgggccg gcgaagccgg agccagccag
     3901 aactgggagg aacataacgt aaaaaagtgc gtctctcttt ctctcgctcc ccgatggttg
     3961 ttttccgacg aaggaacatt gaagtttggg agagttggga tttttgaatc gaactaatca
     4021 atcctcaaaa taattcaata aaaaaataat cctaattata attattaaaa aaaataaaag
     4081 cgacaacaga gttgattttt tgtttgaaaa ataatgttta tgaaatttag tttaaaatgt
     4141 gttttcaata tgtggataaa ttttttaaag cttctttgca ataagaggca tttttagtaa
     4201 caaaacaaca gaacaaaaaa acactttttc attttcacgc cagcaattta tctgaacttt
     4261 ttacgtctgt gacttttcgt ttgacatttg cccagtcagt attttagcga attctaaccg
     4321 agctggacgt tcagaattcc ggctaaaatt tcaaaatgat tttaactgta ttctgataaa
     4381 atcagcattt ggtagaagaa ttcatgaact ttttttctaa agttatacta ctttcagtat
     4441 ttttttgtct ttgtagttat ttatgttaaa atttattttt gatgaaaata aacattgttt
     4501 tcaatttatt taaaatcaat cgattaaagt taatcaagac actactcgtt caccctaccc
     4561 tgtaacacca accgaagtta tccgcgcccg cgaatcgacg aaccacaaca aaacgatcca
     4621 aacaacgata aaggaacctc cagatcccct cataacccgg cccaaacccc gtgtacgaat
     4681 cagctgctgt ggggcgaagt ttttccgact tgtgccggcg acgacggcga ttttcgccgc
     4741 atttgctcca cttttggcag aataatgctg ccataattcc atttttggaa tttgtcgagg
     4801 ccaattaatt gccttatgcg agccaaggtt ctataaattg gtggtgcaga taattccaag
     4861 atgctctact cgaagtggct gtctatgtgt ttagatggtt tcaagaatat tggtcaaatt
     4921 ttaagaggaa gctaactcta tttgattttt tttggaaagt catttgggga atccaagaaa
     4981 catgtatttt tgaacttata atttaaaaaa acccatcctc caaggaaaag gttttatttt
     5041 tcaatcgttg ttagaacatg tttttagaac ccccaaaatg actgtccatt gattggttga
     5101 ccaattgagt ctaccaattc ttcctcctcc gggtttttga ttctggaaat taaaaggaac
     5161 cctagaattt aaaaaaaaat gtttttccta attgtttcgc ttgtgtagtc cagttataac
     5221 acttatttat tattttttca tcaaatttat cgttgaattt tcattgtaca gattgtttgc
     5281 attatttgga ggaggactag caactttttt ttggaggggt attttggagg ggtattttgg
     5341 aggagtattt tagaggggta ttttggaggg gtatctgtaa ggggtatctg taaggaaatt
     5401 ttctgatcaa tttggtgtct tcggcaaagt tgtaggtatt gctgaggact attgagaaaa
     5461 aaatttggtc acggaaaata aattgccgat ttttttattg actttttttt tcacaaaaac
     5521 tcaatttccg aaaataacgt attttttgat tttcgagatt ttttgatatg ttttagagaa
     5581 caaaaacccg catcttttga accatagaga agtatggtca aaaaaatctg ccaccgagtt
     5641 atagtgatag tgatttttga aaaaaaaaca gagatttttt aaaaaaaatc taaattttat
     5701 gcaaaaaatt cgacctaatt ttttcattgt gaaattgaat ttgcaatcga aaagtacttt
     5761 acacattttt tgataaaggg ctccgttttt aaaatatagc cactgaaagt ttgattttag
     5821 cgaaatatta acggtttttc gatatttaca aaaagggacc atgagtgacc atttccaaaa
     5881 atattttttt tgaaaagttc aaaaattaaa aaaaaaatat aagagagacg ttgaaggttg
     5941 gacctatggt tgctttaaag ctagaagtcg cctgacaaaa aactttttct agaaacagat
     6001 aggaaacctg atgcaaacaa acctccagct gccatgtaaa catattttca atgtgttggt
     6061 aaatttctga ctagaatatc ttacacctta accaaaaatc atgattttct gagttcaata
     6121 tcccactttt catacgaatt gttcagttca acaaatatta ccatttttat ggttgtagta
     6181 cctgaactca aaaaatcgta tgaaaagtgg gatattgaac tcagaaaatc atgattttta
     6241 gtaagggagt acggcattct agacccagta aaaaaaatat aattcaaccc ataaatgacg
     6301 aacttctcgt cacagtgtgc tgatgcaaac aatgatcgag ctcgagctgt cacaaccctg
     6361 cgaagtatgt tggccaacat atcgggctga cagtcaaaaa accacctaga atttgcatga
     6421 cacaaaactt ttgctagaaa aagataggaa acctgatgca aacaaacgtc cagctgccat
     6481 gtaaacatac tttgaatgtg taagtaaatt tctgactaga aagccttatt taaagtccaa
     6541 taaacaacac ttttctatct ccacgtacgc ccggtggccg atgtcgtaca tgagaaacaa
     6601 tttaaaaaaa acaacgtgtt atcttgtaag taattaaaca cggaatggct ttactgaatc
     6661 catttgacaa ccggaacctc ctctacgtaa tgcgctgtcg tgacctgggc gttccagacg
     6721 ccaagttcga gtccagtggt gtgcggactg gtggggacca tcgctgataa ggttgtccca
     6781 acaaacactt tcattttttg cttttagggt gttttagaat atctctgaca caaggcgatt
     6841 taaaaaaaac actccagctc tatttttttt tatttcgacg gtgacatttc aaaaggcatc
     6901 atgtacattt tgacactttc tgggttattg catattctaa aagtactcct aataagctac
     6961 ctttccacca aaaatgagca aaagttactt cagtaaagtc tgtttaatca tgatttgaaa
     7021 taaagtaaca taaacaaaat ctctgccatc agcagtccct gtttatatag ccctgtcaac
     7081 ctgtcaaaca aaagactaca taaacctcgt gacgaaaaaa aagctacatg aacaaagcgc
     7141 catgtacatt cggcgaagaa aaacgaatca taacaaagcg tccccctcaa tgacagcagg
     7201 gtgatataga cgttggtgat ttcaaaagga gttatgtttg tttttcttaa atataacgat
     7261 ggaaataatg ttttattgaa tttggatgat caagtatcaa tgaatgcaac gtttttcatc
     7321 gtttgttgtt attagaacat cttattttgc ttttatatta aaatgggaat tgaaaataga
     7381 tgctcaacat tcaaatgcgt ttttctcaaa acgcatggtt tgtacatgat gctttttgaa
     7441 atgttggcgt cgattttacc gaaagtgact catctggaca tttccagtct gtgactttta
     7501 aggagtttca ctcattttca ttcaacagtg tacgacaaac gtcaaacgaa tttgtgcgtc
     7561 accttcagca aatttcatca ccttgctctt ggagccacgg cagagaggca gctttttcct
     7621 tttggtcctg ttttatcctg atttcgttta ttttctcgta ttttttacat tgtgcttttt
     7681 gtaaaatttg ataactttaa tccctatttg tatgttgtga aaacataaat ctgctaattg
     7741 tgtgcctcaa gtgccgtgaa tttcgcccaa cggtaagaat cgtgtccttt ttttgacgat
     7801 gacgcaaaac ctaacctcaa agttccccct tcccggcaag gtcctttcta aaacatggat
     7861 aagaagtact cgatcggtct ggatatcggt acgaactcgg tgggttgggc cgtgatcacg
     7921 gatgagtaca aggtgccgtc gaagaagttc aaggtgctgg gtaacacgga tcgccactcg
     7981 atcaagaaga acctgatcgg tgccctgctg ttcgattcgg gtgagacggc cgaggccacg
     8041 cgcctgaagc gcacggcccg ccgccgctac acgcgccgca agaaccgcat ctgctacctg
     8101 caggagatct tctcgaacga gatggccaag gtggatgatt cgttcttcca ccgcctggag
     8161 gagtcgttcc tggtggagga ggataagaag cacgagcgcc acccgatctt cggtaacatc
     8221 gtggatgagg tggcctacca cgagaagtac ccgacgatct accacctgcg caagaagctg
     8281 gtggattcga cggataaggc cgatctgcgc ctgatctacc tggccctggc ccacatgatc
     8341 aagttccgcg gtcacttcct gatcgagggt gatctgaacc cggataactc ggatgtggat
     8401 aagctgttca tccagctggt gcagacgtac aaccagctgt tcgaggagaa cccgatcaac
     8461 gcctcgggtg tggatgccaa ggccatcctg tcggcccgcc tgtcgaagtc gcgccgcctg
     8521 gagaacctga tcgcccagct gccgggtgag aagaagaacg gtctgttcgg taacctgatc
     8581 gccctgtcgc tgggtctgac gccgaacttc aagtcgaact tcgatctggc cgaggatgcc
     8641 aagctgcagc tgtcgaagga tacgtacgat gatgatctgg ataacctgct ggcccagatc
     8701 ggtgatcagt acgccgatct gttcctggcc gccaagaacc tgtcggatgc catcctgctg
     8761 tcggatatcc tgcgcgtgaa cacggagatc acgaaggccc cgctgtcggc ctcgatgatc
     8821 aagcgctacg atgagcacca ccaggatctg acgctgctga aggccctggt gcgccagcag
     8881 ctgccggaga agtacaagga gatcttcttc gatcagtcga agaacggtta cgccggttac
     8941 atcgatggtg gtgcctcgca ggaggagttc tacaagttca tcaagccgat cctggagaag
     9001 atggatggta cggaggagct gctggtgaag ctgaaccgcg aggatctgct gcgcaagcag
     9061 cgcacgttcg ataacggttc gatcccgcac cagatccacc tgggtgagct gcacgccatc
     9121 ctgcgccgcc aggaggattt ctacccgttc ctgaaggata accgcgagaa gatcgagaag
     9181 atcctgacgt tccgcatccc gtactacgtg ggtccgctgg cccgcggtaa ctcgcgcttc
     9241 gcctggatga cgcgcaagtc ggaggagacg atcacgccgt ggaacttcga ggaggtggtg
     9301 gataagggtg cctcggccca gtcgttcatc gagcgcatga cgaacttcga taagaacctg
     9361 ccgaacgaga aggtgctgcc gaagcactcg ctgctgtacg agtacttcac ggtgtacaac
     9421 gagctgacga aggtgaagta cgtgacggag ggtatgcgca agccggcctt cctgtcgggt
     9481 gagcagaaga aggccatcgt ggatctgctg ttcaagacga accgcaaggt gacggtgaag
     9541 cagctgaagg aggattactt caagaagatc gagtgcttcg attcggtgga gatctcgggt
     9601 gtggaggatc gcttcaacgc ctcgctgggt acgtaccacg atctgctgaa gatcatcaag
     9661 gataaggatt tcctggataa cgaggagaac gaggatatcc tggaggatat cgtgctgacg
     9721 ctgacgctgt tcgaggatcg cgagatgatc gaggagcgcc tgaagacgta cgcccacctg
     9781 ttcgatgata aggtgatgaa gcagctgaag cgccgccgct acacgggttg gggtcgcctg
     9841 tcgcgcaagc tgatcaacgg tatccgcgat aagcagtcgg gtaagacgat cctggatttc
     9901 ctgaagtcgg atggtttcgc caaccgcaac ttcatgcagc tgatccacga tgattcgctg
     9961 acgttcaagg aggatatcca gaaggcccag gtgtcgggtc agggtgattc gctgcacgag
    10021 cacatcgcca acctggccgg ttcgccggcc atcaagaagg gtatcctgca gacggtgaag
    10081 gtggtggatg agctggtgaa ggtgatgggt cgccacaagc cggagaacat cgtgatcgag
    10141 atggcccgcg agaaccagac gacgcagaag ggtcagaaga actcgcgcga gcgcatgaag
    10201 cgcatcgagg agggtatcaa ggagctgggt tcgcagatcc tgaaggagca cccggtggag
    10261 aacacgcagc tgcagaacga gaagctgtac ctgtactacc tgcagaacgg tcgcgatatg
    10321 tacgtggatc aggagctgga tatcaaccgc ctgtcggatt acgatgtgga tcacatcgtg
    10381 ccgcagtcgt tcctgaagga tgattcgatc gataacaagg tgctgacgcg ctcggataag
    10441 aaccgcggta agtcggataa cgtgccgtcg gaggaggtgg tgaagaagat gaagaactac
    10501 tggcgccagc tgctgaacgc caagctgatc acgcagcgca agttcgataa cctgacgaag
    10561 gccgagcgcg gtggtctgtc ggagctggat aaggccggtt tcatcaagcg ccagctggtg
    10621 gagacgcgcc agatcacgaa gcacgtggcc cagatcctgg attcgcgcat gaacacgaag
    10681 tacgatgaga acgataagct gatccgcgag gtgaaggtga tcacgctgaa gtcgaagctg
    10741 gtgtcggatt tccgcaagga tttccagttc tacaaggtgc gcgagatcaa caactaccac
    10801 cacgcccacg atgcctacct gaacgccgtg gtgggtacgg ccctgatcaa gaagtacccg
    10861 aagctggagt cggagttcgt gtacggtgat tacaaggtgt acgatgtgcg caagatgatc
    10921 gccaagtcgg agcaggagat cggtaaggcc acggccaagt acttcttcta ctcgaacatc
    10981 atgaacttct tcaagacgga gatcacgctg gccaacggtg agatccgcaa gcgcccgctg
    11041 atcgagacga acggtgagac gggtgagatc gtgtgggata agggtcgcga tttcgccacg
    11101 gtgcgcaagg tgctgtcgat gccgcaggtg aacatcgtga agaagacgga ggtgcagacg
    11161 ggtggtttct cgaaggagtc gatcctgccg aagcgcaact cggataagct gatcgcccgc
    11221 aagaaggatt gggatccgaa gaagtacggt ggtttcgatt cgccgacggt ggcctactcg
    11281 gtgctggtgg tggccaaggt ggagaagggt aagtcgaaga agctgaagtc ggtgaaggag
    11341 ctgctgggta tcacgatcat ggagcgctcg tcgttcgaga agaacccgat cgatttcctg
    11401 gaggccaagg gttacaagga ggtgaagaag gatctgatca tcaagctgcc gaagtactcg
    11461 ctgttcgagc tggagaacgg tcgcaagcgc atgctggcct cggccggtga gctgcagaag
    11521 ggtaacgagc tggccctgcc gtcgaagtac gtgaacttcc tgtacctggc ctcgcactac
    11581 gagaagctga agggttcgcc ggaggataac gagcagaagc agctgttcgt ggagcagcac
    11641 aagcactacc tggatgagat catcgagcag atctcggagt tctcgaagcg cgtgatcctg
    11701 gccgatgcca acctggataa ggtgctgtcg gcctacaaca agcaccgcga taagccgatc
    11761 cgcgagcagg ccgagaacat catccacctg ttcacgctga cgaacctggg tgccccggcc
    11821 gccttcaagt acttcgatac gacgatcgat cgcaagcgct acacgtcgac gaaggaggtg
    11881 ctggatgcca cgctgatcca ccagtcgatc acgggtctgt acgagacgcg catcgatctg
    11941 tcgcagctgg gtggtgatgg ttcgccgaag aagaagcgca aggtgtaaaa ttgtagtaat
    12001 ttgaaccatg taagagaaaa aagaaactgt cgaatcggtt tcgctgcctg gcgcgcggaa
    12061 atcggcataa agctaggtgg aaagaaggtt tgttccggaa aaagtgtcat tttgtttaca
    12121 cgtcgaatgt gtattcttgt ttttttaacg cactttttat actgtttttt ttttgtactc
    12181 ttatttgtta ctgtataatc gagacttgta taagaaaaag cgttatgttc tgactatgat
    12241 gaattcaata aaatgatgtt cgaacgaatt tctcagaaaa gttattatct acgtgtccat
    12301 atttttttaa acatttaaaa aaaggtcaac ttttcagaat tcaaataagc tcgttgatta
    12361 actgttggag caaaccatga aatgtgacaa agttagggaa taaaaatatt tcgtctctct
    12421 gtaagcaaag gaaaagtaaa ttgtgaaaag taaaaatatg ttctaccaca aatttaaatt
    12481 ttcattggaa aaaacttgta cacgaaaagt tcacgagaga tgggcaaaca aaaagagaaa
    12541 gtcgctcaaa gagccggttt actaaaagag ctaacgaacc atggctcaca aaaaaaaccg
    12601 cgattatttt tgaaactccg atcttttgaa agaaccgttc caagatgtaa tgattttgaa
    12661 aatgtgtgtc tatttttatg tttatttagg ctggtataat agtagtttat gcaacaagtt
    12721 gcaaaaagag gattttttca gcacgagtcg tacatttatc caacgaggtt caccgagttg
    12781 gataaatacg aagagtgctg aaaaaatcaa gttttgcaac gagttccata caacattttt
    12841 tgcaattccg aaaaataccc aatgagtgaa atttaatgtc aaattttcat gtattttgtc
    12901 aataaatcgt tgaaatcaaa aaaatgttga aaagtgttac ttttcgaaac aagtgctgaa
    12961 aagtagaact tttcagcatt tattttgaaa agtgttgcta ttcgattctg ttatttttgg
    13021 tacagaaaag tatgctattt cgtcgttcaa gaatgacagg aaaagtaagt agtttcacga
    13081 cggaatcgca aaaattttat tttaaaaaca tataaaacgt gcaataagct aaaatttatt
    13141 taaaatgcat tccgctgcgt ttaaaaatat gtttagcatg tttgaattga tttaaaaatc
    13201 ttttcaattt ttgaaaaaaa tcgatgttta gtatcgcaaa aagttttttt ttgcattttt
    13261 tttgttttcg tcaaatctta cttttttttg aaaactaatg attgcattta aaaaaaattg
    13321 cattcaaatg ttggcttgtt atttcaattt ttaatttttt ttcagacaaa ttttgagcaa
    13381 ccttgctcaa gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
    13441 ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
    13501 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    13561 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    13621 gcagcctgaa tggcgaatgg
//
