LOCUS       Exported               11070 bp ds-DNA     circular SYN 06-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   StruBiol
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 24, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1821..2681
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3764..3794
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3874..3907
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3916..3945
                     /codon_start=1
                     /label=10xlinker
                     /translation="GGSGGSGSGA"
     CDS             3946..4632
                     /codon_start=1
                     /label=auxin-inducible degron
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4651..4740
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    4776..5308
                     /label=S. pombe urg1 teminator sequence
     gene            5354..6710
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5354..5697
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5698..6507
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      6513..6710
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6804..6820)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9719..10813
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggtgctgg agcaggcgcc tacccatacg atgttcctga ctatgcgggc
     4681 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     4741 gctcagtgct gaggcgcgcc gctagcagtc ccggggcttg cccatctgtt ttagacgtca
     4801 cagaaggacc atgtttattt taaatttcac atacatgtgt ttaattacct tcattgtata
     4861 aattctcgaa actttctcca attcttcggt cagataaaaa atttctctag tcaccgtctt
     4921 ttatagagct tttgtcagat gtttttctgg atggaatcat tgtccagtta ctatgctttt
     4981 tatttataag tttgaaagtt tagttaactt tttgttttct ttaatacatt ttttaatcat
     5041 gtcaacattt gggatttgta gaagtagtaa attttttgta catgttgtag aattggataa
     5101 gaacgaaaac agctagtatg taaaaaaaac gaaaatccca aattccgttg tttgtttgat
     5161 atagtaatat ttatagcatt ttagtttcta aagatttttg ataatctttt acatttgctt
     5221 tcacatacat tcgcgctact accatacatc atatcccgct ttctttaatt gcttccccac
     5281 agactcaacc agcggggcct tcttctcgag atctgtttag cttgcctcgt ccccgccggg
     5341 tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag
     5401 ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc
     5461 atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct
     5521 gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc
     5581 gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact
     5641 tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg
     5701 ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta
     5761 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
     5821 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     5881 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     5941 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc
     6001 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     6061 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     6121 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     6181 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     6241 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     6301 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     6361 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     6421 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     6481 catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt cttgttttca
     6541 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
     6601 gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc
     6661 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc
     6721 gatactaacg ccgccatcca gtcgacgaac tagtataact tcgtatatgg tattatatac
     6781 gaagttatgc ggccgctgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6841 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6901 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6961 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataccatcg
     7021 tccggatcaa tgaagtagat gagtttacta cctgtatata cttttttttt tgtcaaagtc
     7081 acttttatgg catttccatg ttgaaggatt agccacagca ctaaattttc agcgtatgat
     7141 tgcttttaaa tatttaattt tcatcgtttt ttaattaata ttcaaaacga tttaatgcct
     7201 tctacatctg aagtcaagct catagactcg ggttgggtag ctaaaactac atctaacacg
     7261 aaggggtata gccttcttaa agtatactca tgtataaacg ttttgggttc atatttttgg
     7321 gccacgtggt ttataaaaat tcttaactac accactcggt atcccgcacc cgtctacgtt
     7381 tctacgattt cgagctaata ttgagtaaag tgattagcaa aaaaaataaa agtactcgtt
     7441 gtcggagatc aagaattttt ctattatctc atctaaacca ctttctaaaa gcgaaaaaca
     7501 aaatcgtaat atgcagcttg aatgggcttc catagtttga aagaaaaacc ctagcagtac
     7561 tggcaaggga gacattcctt ttacctggct aatcgccatc ttccagcagg cgcaccattg
     7621 cccctgtttc actatccagg ttacggatat agttcatgac aatatttaca ttggtccagc
     7681 caccagcttg catgatctcc ggtattgaaa ctccagcgcg ggccatatct cgcgcggctc
     7741 cgacacgggc actgtgtcca gaccaggcca ggtatctctg accagagtca tccttagcgc
     7801 cgtaaatcaa tcgatgagtt gcttcaaaaa tcccttccag ggcgcgagtt gatagctggc
     7861 tggtggcaga tggcgcggca acaccatttt ttctgacccg gcaaaacagg tagttattcg
     7921 gatcatcagc tacaccagag acggaaatcc atcgctcgac cagtttagtt acccccaggc
     7981 taagtgcctt ctctacacct gcggtgctaa ccagcgtttt cgttctgcca atatggatta
     8041 acattctccc accgtcagta cgtgagatat ctttaaccct gatcctggca atttcggcta
     8101 tacgtaacag ggtgttataa gcaatcccca gaaatgccag attacgtata tcctggcagc
     8161 gatcgctatt ttccatgagt gaacgaacct ggtcgaaatc agtgcgttcg aacgctagag
     8221 cctgttttgc acgttcaccg gcatcaacgt tttcttttcg gatccgccgc ataaccagtg
     8281 aaacagcatt gctgtcactt ggtcgtggca gcccggaccg acgatgaagc atgtttagct
     8341 ggcccaaatg ttgctggata gtttttactg ccagaccgcg cgcctgaaga tatagaagat
     8401 aatcgcgaac atcttcaggt tctgcgggaa accatttccg gttattcaac ttgcaccatg
     8461 ccgcccacga ccggcaaacg gacagaagca ttttccaggt atgctcagaa aacgcctggc
     8521 gatccctgaa catgtccatc aggttcttgc gaacctcatc actcgttgca tcgaccggta
     8581 acttttagat taacatttgt ttaaatatga aattaggcaa tgtaacatac tgcaggcaaa
     8641 ttttggtgta cggtaagtaa attggacata tgatttaaca aagcgactat aagtcagaaa
     8701 gtgagaatga gattgaaata attaattcaa cttattcaat tgatgatatg ccaggattcc
     8761 tcttccttta tatatcttca atgtttcgat taacaggtcc attatccggt ttttagtttc
     8821 gctgcacatt gccgaatgac aatcggattc ctttaagctt tgcttttgcc ctgttttctc
     8881 tccccagtaa tcgttgggtg actgaaccat ccagtagccg agagttacgt ttttcctgat
     8941 ttaataaata tccatatcta tttgtctcgt ggtgttttac agcaactaac cgaaattact
     9001 attttgcatc atccaaccat aaagagacaa caataattct agaaaacata taacaagtgt
     9061 ttttttctat ttacgtctca tacgcaaagt ccactttacc atgttttgga aacttgtacc
     9121 caaccattca tttttcgatt ttgtaatgag gctcttttct tctaacaatc attgaggtca
     9181 ttatcatgtg tcctcaccta ttgtttaaat tgaggcttgc gctattccca aaattgtggg
     9241 tattattttc atatatcagt atctgcaatt tcggttcaac tccggaagct cggtacccaa
     9301 ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc aaccattatt
     9361 ttttttctct caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat
     9421 gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa
     9481 aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga
     9541 agcgttcatg actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac
     9601 ctattgtttt ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt
     9661 tacatttcag caatatatat atatatatat atatatattt caaggatata ccattgtaat
     9721 gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag aaatcacagc
     9781 cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca agttcgattt
     9841 cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac ttccagatga
     9901 ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa
     9961 atggggtacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta aagaacttca
    10021 attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag acttatctcc
    10081 aatcaagcca caatttgcta aaggtactga cttcgttgtt gttagagaat tagtgggagg
    10141 tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg atagtgaaca
    10201 atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg ccctacaaca
    10261 tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct cttcaagatt
    10321 atggagaaaa actgtggagg aaaccatcaa gaacgaattt cctacattga aagttcaaca
    10381 tcaattgatt gattctgccg ccatgatcct agttaagaac ccaacccacc taaatggtat
    10441 tataatcacc agcaacatgt ttggtgatat catctccgat gaagcctccg ttatcccagg
    10501 ctccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt
    10561 tggtttgtac gaaccatgcc atggttccgc tccagatttg ccaaagaata aggtcaaccc
    10621 tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact tgcctgaaga
    10681 aggtaaagcc attgaagatg cagttaaaaa ggttttggat gcaggtatca gaactggtga
    10741 tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa
    10801 aatccttgct taaaaagatt ctcttttttt gtgatatttg tacataaact ttataaatga
    10861 aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg gtagacgaaa
    10921 ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg taaaggaata
    10981 caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt gcactttaac
    11041 attaatattg acaaggagga gggcatcaca
//
