LOCUS       Exported               11151 bp ds-DNA     circular SYN 06-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   StruBiol
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 24, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1821..2681
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3764..3794
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3874..3907
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3916..3945
                     /codon_start=1
                     /label=10xlinker
                     /translation="GGSGGSGSGA"
     CDS             3946..4632
                     /codon_start=1
                     /label=auxin-inducible degron
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4648..4677
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4684..4713
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4720..4749
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4765..4794
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4801..4830
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    4857..5389
                     /label=S. pombe urg1 teminator sequence
     gene            5435..6791
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5435..5778
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5779..6588
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      6594..6791
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6885..6901)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9800..10894
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggagcagg cgccggtgaa caaaagttga tttctgaaga agatttgaac
     4681 ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa
     4741 gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga tttgaacggt
     4801 gaacaaaagc taatctccga ggaagacttg taaggcgcgc cgctagcagt cccggggctt
     4861 gcccatctgt tttagacgtc acagaaggac catgtttatt ttaaatttca catacatgtg
     4921 tttaattacc ttcattgtat aaattctcga aactttctcc aattcttcgg tcagataaaa
     4981 aatttctcta gtcaccgtct tttatagagc ttttgtcaga tgtttttctg gatggaatca
     5041 ttgtccagtt actatgcttt ttatttataa gtttgaaagt ttagttaact ttttgttttc
     5101 tttaatacat tttttaatca tgtcaacatt tgggatttgt agaagtagta aattttttgt
     5161 acatgttgta gaattggata agaacgaaaa cagctagtat gtaaaaaaaa cgaaaatccc
     5221 aaattccgtt gtttgtttga tatagtaata tttatagcat tttagtttct aaagattttt
     5281 gataatcttt tacatttgct ttcacataca ttcgcgctac taccatacat catatcccgc
     5341 tttctttaat tgcttcccca cagactcaac cagcggggcc ttcttctcga gatctgttta
     5401 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     5461 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     5521 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     5581 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     5641 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     5701 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     5761 atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat
     5821 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     5881 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     5941 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     6001 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     6061 ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca
     6121 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     6181 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     6241 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     6301 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     6361 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     6421 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     6481 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     6541 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agtactgaca
     6601 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     6661 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     6721 cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg
     6781 tcgctatact gctgtcgatt cgatactaac gccgccatcc agtcgacgaa ctagtataac
     6841 ttcgtatatg gtattatata cgaagttatg cggccgctga attcactggc cgtcgtttta
     6901 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6961 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     7021 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     7081 atttcacacc gcataccatc gtccggatca atgaagtaga tgagtttact acctgtatat
     7141 actttttttt ttgtcaaagt cacttttatg gcatttccat gttgaaggat tagccacagc
     7201 actaaatttt cagcgtatga ttgcttttaa atatttaatt ttcatcgttt tttaattaat
     7261 attcaaaacg atttaatgcc ttctacatct gaagtcaagc tcatagactc gggttgggta
     7321 gctaaaacta catctaacac gaaggggtat agccttctta aagtatactc atgtataaac
     7381 gttttgggtt catatttttg ggccacgtgg tttataaaaa ttcttaacta caccactcgg
     7441 tatcccgcac ccgtctacgt ttctacgatt tcgagctaat attgagtaaa gtgattagca
     7501 aaaaaaataa aagtactcgt tgtcggagat caagaatttt tctattatct catctaaacc
     7561 actttctaaa agcgaaaaac aaaatcgtaa tatgcagctt gaatgggctt ccatagtttg
     7621 aaagaaaaac cctagcagta ctggcaaggg agacattcct tttacctggc taatcgccat
     7681 cttccagcag gcgcaccatt gcccctgttt cactatccag gttacggata tagttcatga
     7741 caatatttac attggtccag ccaccagctt gcatgatctc cggtattgaa actccagcgc
     7801 gggccatatc tcgcgcggct ccgacacggg cactgtgtcc agaccaggcc aggtatctct
     7861 gaccagagtc atccttagcg ccgtaaatca atcgatgagt tgcttcaaaa atcccttcca
     7921 gggcgcgagt tgatagctgg ctggtggcag atggcgcggc aacaccattt tttctgaccc
     7981 ggcaaaacag gtagttattc ggatcatcag ctacaccaga gacggaaatc catcgctcga
     8041 ccagtttagt tacccccagg ctaagtgcct tctctacacc tgcggtgcta accagcgttt
     8101 tcgttctgcc aatatggatt aacattctcc caccgtcagt acgtgagata tctttaaccc
     8161 tgatcctggc aatttcggct atacgtaaca gggtgttata agcaatcccc agaaatgcca
     8221 gattacgtat atcctggcag cgatcgctat tttccatgag tgaacgaacc tggtcgaaat
     8281 cagtgcgttc gaacgctaga gcctgttttg cacgttcacc ggcatcaacg ttttcttttc
     8341 ggatccgccg cataaccagt gaaacagcat tgctgtcact tggtcgtggc agcccggacc
     8401 gacgatgaag catgtttagc tggcccaaat gttgctggat agtttttact gccagaccgc
     8461 gcgcctgaag atatagaaga taatcgcgaa catcttcagg ttctgcggga aaccatttcc
     8521 ggttattcaa cttgcaccat gccgcccacg accggcaaac ggacagaagc attttccagg
     8581 tatgctcaga aaacgcctgg cgatccctga acatgtccat caggttcttg cgaacctcat
     8641 cactcgttgc atcgaccggt aacttttaga ttaacatttg tttaaatatg aaattaggca
     8701 atgtaacata ctgcaggcaa attttggtgt acggtaagta aattggacat atgatttaac
     8761 aaagcgacta taagtcagaa agtgagaatg agattgaaat aattaattca acttattcaa
     8821 ttgatgatat gccaggattc ctcttccttt atatatcttc aatgtttcga ttaacaggtc
     8881 cattatccgg tttttagttt cgctgcacat tgccgaatga caatcggatt cctttaagct
     8941 ttgcttttgc cctgttttct ctccccagta atcgttgggt gactgaacca tccagtagcc
     9001 gagagttacg tttttcctga tttaataaat atccatatct atttgtctcg tggtgtttta
     9061 cagcaactaa ccgaaattac tattttgcat catccaacca taaagagaca acaataattc
     9121 tagaaaacat ataacaagtg tttttttcta tttacgtctc atacgcaaag tccactttac
     9181 catgttttgg aaacttgtac ccaaccattc atttttcgat tttgtaatga ggctcttttc
     9241 ttctaacaat cattgaggtc attatcatgt gtcctcacct attgtttaaa ttgaggcttg
     9301 cgctattccc aaaattgtgg gtattatttt catatatcag tatctgcaat ttcggttcaa
     9361 ctccggaagc tcggtaccca actgtgggaa tactcaggta tcgtaagatg caagagttcg
     9421 aatctcttag caaccattat tttttttctc tcaacataac gagaacacac aggggcgcta
     9481 tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg tcgcctgacg
     9541 catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc gccggaaccg
     9601 gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag
     9661 ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac
     9721 tttctaactt ttcttacctt ttacatttca gcaatatata tatatatata tatatatatt
     9781 tcaaggatat accattgtaa tgtctgcccc taagaagatc gtcgttttgc caggtgacca
     9841 cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg
     9901 ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac
     9961 aggtgttcca cttccagatg aggcgctgga agcctccaag aaggctgatg ccgttttgtt
    10021 aggtgctgtg ggtggtccta aatggggtac cggtagtgtt agacctgaac aaggtttact
    10081 aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga
    10141 ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt
    10201 tgttagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg atggtgatgg
    10261 tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc
    10321 cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa
    10381 tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt
    10441 tcctacattg aaagttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa
    10501 cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata tcatctccga
    10561 tgaagcctcc gttatcccag gctccttggg tttgttgcca tctgcgtcct tggcctcttt
    10621 gccagacaag aacaccgcat ttggtttgta cgaaccatgc catggttccg ctccagattt
    10681 gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt
    10741 gtcattgaac ttgcctgaag aaggtaaagc cattgaagat gcagttaaaa aggttttgga
    10801 tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc
    10861 tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt tgtgatattt
    10921 gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac aaaatggaat
    10981 atgttcatag ggtagacgaa actatatacg caatctacat acatttatca agaaggagaa
    11041 aaaggaggat gtaaaggaat acaggtaagc aaattgatac taatggctca acgtgataag
    11101 gaaaaagaat tgcactttaa cattaatatt gacaaggagg agggcatcac a
//
