LOCUS       Exported               11005 bp ds-DNA     circular SYN 06-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   StruBiol
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 24, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1821..2681
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3764..3794
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3874..3907
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3916..3945
                     /codon_start=1
                     /label=10xlinker
                     /translation="GGSGGSGSGA"
     CDS             3946..4632
                     /codon_start=1
                     /label=auxin-inducible degron
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4651..4692
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4711..5243
                     /label=S. pombe urg1 teminator sequence
     gene            5289..6645
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5289..5632
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5633..6442
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      6448..6645
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6739..6755)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9654..10748
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggtgctgg agcaggcgcc ggcaaaccga tccctaaccc actcctgggc
     4681 ctggactcaa catgagctag cagtcccggg gcttgcccat ctgttttaga cgtcacagaa
     4741 ggaccatgtt tattttaaat ttcacataca tgtgtttaat taccttcatt gtataaattc
     4801 tcgaaacttt ctccaattct tcggtcagat aaaaaatttc tctagtcacc gtcttttata
     4861 gagcttttgt cagatgtttt tctggatgga atcattgtcc agttactatg ctttttattt
     4921 ataagtttga aagtttagtt aactttttgt tttctttaat acatttttta atcatgtcaa
     4981 catttgggat ttgtagaagt agtaaatttt ttgtacatgt tgtagaattg gataagaacg
     5041 aaaacagcta gtatgtaaaa aaaacgaaaa tcccaaattc cgttgtttgt ttgatatagt
     5101 aatatttata gcattttagt ttctaaagat ttttgataat cttttacatt tgctttcaca
     5161 tacattcgcg ctactaccat acatcatatc ccgctttctt taattgcttc cccacagact
     5221 caaccagcgg ggccttcttc tcgagatctg tttagcttgc ctcgtccccg ccgggtcacc
     5281 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
     5341 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
     5401 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
     5461 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
     5521 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
     5581 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa
     5641 ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     5701 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     5761 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     5821 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     5881 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
     5941 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     6001 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     6061 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     6121 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     6181 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     6241 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     6301 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     6361 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     6421 gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt tttcaagaac
     6481 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
     6541 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
     6601 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
     6661 taacgccgcc atccagtcga cgaactagta taacttcgta tatggtatta tatacgaagt
     6721 tatgcggccg ctgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6781 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6841 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     6901 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac catcgtccgg
     6961 atcaatgaag tagatgagtt tactacctgt atatactttt ttttttgtca aagtcacttt
     7021 tatggcattt ccatgttgaa ggattagcca cagcactaaa ttttcagcgt atgattgctt
     7081 ttaaatattt aattttcatc gttttttaat taatattcaa aacgatttaa tgccttctac
     7141 atctgaagtc aagctcatag actcgggttg ggtagctaaa actacatcta acacgaaggg
     7201 gtatagcctt cttaaagtat actcatgtat aaacgttttg ggttcatatt tttgggccac
     7261 gtggtttata aaaattctta actacaccac tcggtatccc gcacccgtct acgtttctac
     7321 gatttcgagc taatattgag taaagtgatt agcaaaaaaa ataaaagtac tcgttgtcgg
     7381 agatcaagaa tttttctatt atctcatcta aaccactttc taaaagcgaa aaacaaaatc
     7441 gtaatatgca gcttgaatgg gcttccatag tttgaaagaa aaaccctagc agtactggca
     7501 agggagacat tccttttacc tggctaatcg ccatcttcca gcaggcgcac cattgcccct
     7561 gtttcactat ccaggttacg gatatagttc atgacaatat ttacattggt ccagccacca
     7621 gcttgcatga tctccggtat tgaaactcca gcgcgggcca tatctcgcgc ggctccgaca
     7681 cgggcactgt gtccagacca ggccaggtat ctctgaccag agtcatcctt agcgccgtaa
     7741 atcaatcgat gagttgcttc aaaaatccct tccagggcgc gagttgatag ctggctggtg
     7801 gcagatggcg cggcaacacc attttttctg acccggcaaa acaggtagtt attcggatca
     7861 tcagctacac cagagacgga aatccatcgc tcgaccagtt tagttacccc caggctaagt
     7921 gccttctcta cacctgcggt gctaaccagc gttttcgttc tgccaatatg gattaacatt
     7981 ctcccaccgt cagtacgtga gatatcttta accctgatcc tggcaatttc ggctatacgt
     8041 aacagggtgt tataagcaat ccccagaaat gccagattac gtatatcctg gcagcgatcg
     8101 ctattttcca tgagtgaacg aacctggtcg aaatcagtgc gttcgaacgc tagagcctgt
     8161 tttgcacgtt caccggcatc aacgttttct tttcggatcc gccgcataac cagtgaaaca
     8221 gcattgctgt cacttggtcg tggcagcccg gaccgacgat gaagcatgtt tagctggccc
     8281 aaatgttgct ggatagtttt tactgccaga ccgcgcgcct gaagatatag aagataatcg
     8341 cgaacatctt caggttctgc gggaaaccat ttccggttat tcaacttgca ccatgccgcc
     8401 cacgaccggc aaacggacag aagcattttc caggtatgct cagaaaacgc ctggcgatcc
     8461 ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg ttgcatcgac cggtaacttt
     8521 tagattaaca tttgtttaaa tatgaaatta ggcaatgtaa catactgcag gcaaattttg
     8581 gtgtacggta agtaaattgg acatatgatt taacaaagcg actataagtc agaaagtgag
     8641 aatgagattg aaataattaa ttcaacttat tcaattgatg atatgccagg attcctcttc
     8701 ctttatatat cttcaatgtt tcgattaaca ggtccattat ccggttttta gtttcgctgc
     8761 acattgccga atgacaatcg gattccttta agctttgctt ttgccctgtt ttctctcccc
     8821 agtaatcgtt gggtgactga accatccagt agccgagagt tacgtttttc ctgatttaat
     8881 aaatatccat atctatttgt ctcgtggtgt tttacagcaa ctaaccgaaa ttactatttt
     8941 gcatcatcca accataaaga gacaacaata attctagaaa acatataaca agtgtttttt
     9001 tctatttacg tctcatacgc aaagtccact ttaccatgtt ttggaaactt gtacccaacc
     9061 attcattttt cgattttgta atgaggctct tttcttctaa caatcattga ggtcattatc
     9121 atgtgtcctc acctattgtt taaattgagg cttgcgctat tcccaaaatt gtgggtatta
     9181 ttttcatata tcagtatctg caatttcggt tcaactccgg aagctcggta cccaactgtg
     9241 ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt
     9301 tctctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat tcgatgactg
     9361 gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt
     9421 gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat agagaagcgt
     9481 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
     9541 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
     9601 ttcagcaata tatatatata tatatatata tatttcaagg atataccatt gtaatgtctg
     9661 cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc acagccgaag
     9721 ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa
     9781 atcatttaat tggtggtgct gctatcgatg ctacaggtgt tccacttcca gatgaggcgc
     9841 tggaagcctc caagaaggct gatgccgttt tgttaggtgc tgtgggtggt cctaaatggg
     9901 gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt
     9961 acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta tctccaatca
    10021 agccacaatt tgctaaaggt actgacttcg ttgttgttag agaattagtg ggaggtattt
    10081 actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt gaacaataca
    10141 ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta caacatgagc
    10201 caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca agattatgga
    10261 gaaaaactgt ggaggaaacc atcaagaacg aatttcctac attgaaagtt caacatcaat
    10321 tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat ggtattataa
    10381 tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc ccaggctcct
    10441 tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc gcatttggtt
    10501 tgtacgaacc atgccatggt tccgctccag atttgccaaa gaataaggtc aaccctatcg
    10561 ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct gaagaaggta
    10621 aagccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact ggtgatttag
    10681 gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc
    10741 ttgcttaaaa agattctctt tttttgtgat atttgtacat aaactttata aatgaaattc
    10801 ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga cgaaactata
    10861 tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag gaatacaggt
    10921 aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact ttaacattaa
    10981 tattgacaag gaggagggca tcaca
//
