LOCUS       Exported               11224 bp ds-DNA     circular SYN 07-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   StruBiol
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 24, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1821..2681
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3764..3794
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3874..3907
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3916..3945
                     /codon_start=1
                     /label=10xlinker
                     /translation="GGSGGSGSGA"
     CDS             3946..4632
                     /codon_start=1
                     /label=auxin-inducible degron
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4651..4740
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    4767..4923
                     /label=spo5DSR
     misc_feature    4930..5462
                     /label=S. pombe urg1 teminator sequence
     gene            5508..6864
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5508..5851
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5852..6661
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      6667..6864
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6958..6974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9873..10967
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggtgctgg agcaggcgcc tacccatacg atgttcctga ctatgcgggc
     4681 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     4741 gctcagtgct gaggcgcgcc gctagcacta cgccatatca tgcccatccc gctacttctg
     4801 ctgctaatta aatttaaaca tctttccagc atttacttga acatcaaact ctaatatcga
     4861 aatcttttca aacacttatc acatactcaa acttaaacca acataaaacc tgttagacaa
     4921 agccccgggg cttgcccatc tgttttagac gtcacagaag gaccatgttt attttaaatt
     4981 tcacatacat gtgtttaatt accttcattg tataaattct cgaaactttc tccaattctt
     5041 cggtcagata aaaaatttct ctagtcaccg tcttttatag agcttttgtc agatgttttt
     5101 ctggatggaa tcattgtcca gttactatgc tttttattta taagtttgaa agtttagtta
     5161 actttttgtt ttctttaata cattttttaa tcatgtcaac atttgggatt tgtagaagta
     5221 gtaaattttt tgtacatgtt gtagaattgg ataagaacga aaacagctag tatgtaaaaa
     5281 aaacgaaaat cccaaattcc gttgtttgtt tgatatagta atatttatag cattttagtt
     5341 tctaaagatt tttgataatc ttttacattt gctttcacat acattcgcgc tactaccata
     5401 catcatatcc cgctttcttt aattgcttcc ccacagactc aaccagcggg gccttcttct
     5461 cgagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
     5521 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
     5581 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
     5641 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
     5701 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
     5761 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
     5821 gttctcacat cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag
     5881 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     5941 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
     6001 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     6061 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     6121 tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga
     6181 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     6241 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     6301 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     6361 ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc
     6421 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     6481 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     6541 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     6601 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     6661 atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
     6721 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
     6781 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
     6841 atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtcgac
     6901 gaactagtat aacttcgtat atggtattat atacgaagtt atgcggccgc tgaattcact
     6961 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7021 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7081 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     7141 gcatctgtgc ggtatttcac accgcatacc atcgtccgga tcaatgaagt agatgagttt
     7201 actacctgta tatacttttt tttttgtcaa agtcactttt atggcatttc catgttgaag
     7261 gattagccac agcactaaat tttcagcgta tgattgcttt taaatattta attttcatcg
     7321 ttttttaatt aatattcaaa acgatttaat gccttctaca tctgaagtca agctcataga
     7381 ctcgggttgg gtagctaaaa ctacatctaa cacgaagggg tatagccttc ttaaagtata
     7441 ctcatgtata aacgttttgg gttcatattt ttgggccacg tggtttataa aaattcttaa
     7501 ctacaccact cggtatcccg cacccgtcta cgtttctacg atttcgagct aatattgagt
     7561 aaagtgatta gcaaaaaaaa taaaagtact cgttgtcgga gatcaagaat ttttctatta
     7621 tctcatctaa accactttct aaaagcgaaa aacaaaatcg taatatgcag cttgaatggg
     7681 cttccatagt ttgaaagaaa aaccctagca gtactggcaa gggagacatt ccttttacct
     7741 ggctaatcgc catcttccag caggcgcacc attgcccctg tttcactatc caggttacgg
     7801 atatagttca tgacaatatt tacattggtc cagccaccag cttgcatgat ctccggtatt
     7861 gaaactccag cgcgggccat atctcgcgcg gctccgacac gggcactgtg tccagaccag
     7921 gccaggtatc tctgaccaga gtcatcctta gcgccgtaaa tcaatcgatg agttgcttca
     7981 aaaatccctt ccagggcgcg agttgatagc tggctggtgg cagatggcgc ggcaacacca
     8041 ttttttctga cccggcaaaa caggtagtta ttcggatcat cagctacacc agagacggaa
     8101 atccatcgct cgaccagttt agttaccccc aggctaagtg ccttctctac acctgcggtg
     8161 ctaaccagcg ttttcgttct gccaatatgg attaacattc tcccaccgtc agtacgtgag
     8221 atatctttaa ccctgatcct ggcaatttcg gctatacgta acagggtgtt ataagcaatc
     8281 cccagaaatg ccagattacg tatatcctgg cagcgatcgc tattttccat gagtgaacga
     8341 acctggtcga aatcagtgcg ttcgaacgct agagcctgtt ttgcacgttc accggcatca
     8401 acgttttctt ttcggatccg ccgcataacc agtgaaacag cattgctgtc acttggtcgt
     8461 ggcagcccgg accgacgatg aagcatgttt agctggccca aatgttgctg gatagttttt
     8521 actgccagac cgcgcgcctg aagatataga agataatcgc gaacatcttc aggttctgcg
     8581 ggaaaccatt tccggttatt caacttgcac catgccgccc acgaccggca aacggacaga
     8641 agcattttcc aggtatgctc agaaaacgcc tggcgatccc tgaacatgtc catcaggttc
     8701 ttgcgaacct catcactcgt tgcatcgacc ggtaactttt agattaacat ttgtttaaat
     8761 atgaaattag gcaatgtaac atactgcagg caaattttgg tgtacggtaa gtaaattgga
     8821 catatgattt aacaaagcga ctataagtca gaaagtgaga atgagattga aataattaat
     8881 tcaacttatt caattgatga tatgccagga ttcctcttcc tttatatatc ttcaatgttt
     8941 cgattaacag gtccattatc cggtttttag tttcgctgca cattgccgaa tgacaatcgg
     9001 attcctttaa gctttgcttt tgccctgttt tctctcccca gtaatcgttg ggtgactgaa
     9061 ccatccagta gccgagagtt acgtttttcc tgatttaata aatatccata tctatttgtc
     9121 tcgtggtgtt ttacagcaac taaccgaaat tactattttg catcatccaa ccataaagag
     9181 acaacaataa ttctagaaaa catataacaa gtgttttttt ctatttacgt ctcatacgca
     9241 aagtccactt taccatgttt tggaaacttg tacccaacca ttcatttttc gattttgtaa
     9301 tgaggctctt ttcttctaac aatcattgag gtcattatca tgtgtcctca cctattgttt
     9361 aaattgaggc ttgcgctatt cccaaaattg tgggtattat tttcatatat cagtatctgc
     9421 aatttcggtt caactccgga agctcggtac ccaactgtgg gaatactcag gtatcgtaag
     9481 atgcaagagt tcgaatctct tagcaaccat tatttttttt ctctcaacat aacgagaaca
     9541 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag
     9601 aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag
     9661 agcgccggaa ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc
     9721 acaatacttg aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta
     9781 gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat
     9841 atatatatat atttcaagga tataccattg taatgtctgc ccctaagaag atcgtcgttt
     9901 tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta
     9961 tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg
    10021 ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg
    10081 atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg
    10141 aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta
    10201 actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta
    10261 ctgacttcgt tgttgttaga gaattagtgg gaggtattta ctttggtaag agaaaggaag
    10321 acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa
    10381 tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct
    10441 tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca
    10501 tcaagaacga atttcctaca ttgaaagttc aacatcaatt gattgattct gccgccatga
    10561 tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg
    10621 atatcatctc cgatgaagcc tccgttatcc caggctcctt gggtttgttg ccatctgcgt
    10681 ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca tgccatggtt
    10741 ccgctccaga tttgccaaag aataaggtca accctatcgc cactatcttg tctgctgcaa
    10801 tgatgttgaa attgtcattg aacttgcctg aagaaggtaa agccattgaa gatgcagtta
    10861 aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac agtaccaccg
    10921 aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa gattctcttt
    10981 ttttgtgata tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat
    11041 tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta catacattta
    11101 tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc
    11161 tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcat
    11221 caca
//
