LOCUS       Exported               11225 bp ds-DNA     circular SYN 08-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   StruBiol
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 24, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1821..2681
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3764..3794
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3874..3907
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3916..3945
                     /codon_start=1
                     /label=10xlinker
                     /translation="GGSGGSGSGA"
     CDS             3946..4632
                     /codon_start=1
                     /label=auxin-inducible degron
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     CDS             4651..4740
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    4767..4924
                     /label=spo5DSRe
     misc_feature    4931..5463
                     /label=S. pombe urg1 teminator sequence
     gene            5509..6865
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5509..5852
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5853..6662
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      6668..6865
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6959..6975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9874..10968
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag ctggtgctgg agcaggcgcc tacccatacg atgttcctga ctatgcgggc
     4681 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     4741 gctcagtgct gaggcgcgcc gctagcacta cgccatatca tgcccatccc gctacttctg
     4801 ctgctaatta aactttaaac atctttccag catttacttg aacatcaaac tctaatatcg
     4861 aaatcttttc aaacacttat cacatactca aacttaaacc aacattaaac ctgttagaca
     4921 aagccccggg gcttgcccat ctgttttaga cgtcacagaa ggaccatgtt tattttaaat
     4981 ttcacataca tgtgtttaat taccttcatt gtataaattc tcgaaacttt ctccaattct
     5041 tcggtcagat aaaaaatttc tctagtcacc gtcttttata gagcttttgt cagatgtttt
     5101 tctggatgga atcattgtcc agttactatg ctttttattt ataagtttga aagtttagtt
     5161 aactttttgt tttctttaat acatttttta atcatgtcaa catttgggat ttgtagaagt
     5221 agtaaatttt ttgtacatgt tgtagaattg gataagaacg aaaacagcta gtatgtaaaa
     5281 aaaacgaaaa tcccaaattc cgttgtttgt ttgatatagt aatatttata gcattttagt
     5341 ttctaaagat ttttgataat cttttacatt tgctttcaca tacattcgcg ctactaccat
     5401 acatcatatc ccgctttctt taattgcttc cccacagact caaccagcgg ggccttcttc
     5461 tcgagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     5521 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     5581 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     5641 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     5701 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     5761 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     5821 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga
     5881 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     5941 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     6001 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     6061 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     6121 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag
     6181 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     6241 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     6301 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     6361 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     6421 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     6481 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     6541 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     6601 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     6661 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     6721 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     6781 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     6841 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtcga
     6901 cgaactagta taacttcgta tatggtatta tatacgaagt tatgcggccg ctgaattcac
     6961 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7021 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7081 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     7141 cgcatctgtg cggtatttca caccgcatac catcgtccgg atcaatgaag tagatgagtt
     7201 tactacctgt atatactttt ttttttgtca aagtcacttt tatggcattt ccatgttgaa
     7261 ggattagcca cagcactaaa ttttcagcgt atgattgctt ttaaatattt aattttcatc
     7321 gttttttaat taatattcaa aacgatttaa tgccttctac atctgaagtc aagctcatag
     7381 actcgggttg ggtagctaaa actacatcta acacgaaggg gtatagcctt cttaaagtat
     7441 actcatgtat aaacgttttg ggttcatatt tttgggccac gtggtttata aaaattctta
     7501 actacaccac tcggtatccc gcacccgtct acgtttctac gatttcgagc taatattgag
     7561 taaagtgatt agcaaaaaaa ataaaagtac tcgttgtcgg agatcaagaa tttttctatt
     7621 atctcatcta aaccactttc taaaagcgaa aaacaaaatc gtaatatgca gcttgaatgg
     7681 gcttccatag tttgaaagaa aaaccctagc agtactggca agggagacat tccttttacc
     7741 tggctaatcg ccatcttcca gcaggcgcac cattgcccct gtttcactat ccaggttacg
     7801 gatatagttc atgacaatat ttacattggt ccagccacca gcttgcatga tctccggtat
     7861 tgaaactcca gcgcgggcca tatctcgcgc ggctccgaca cgggcactgt gtccagacca
     7921 ggccaggtat ctctgaccag agtcatcctt agcgccgtaa atcaatcgat gagttgcttc
     7981 aaaaatccct tccagggcgc gagttgatag ctggctggtg gcagatggcg cggcaacacc
     8041 attttttctg acccggcaaa acaggtagtt attcggatca tcagctacac cagagacgga
     8101 aatccatcgc tcgaccagtt tagttacccc caggctaagt gccttctcta cacctgcggt
     8161 gctaaccagc gttttcgttc tgccaatatg gattaacatt ctcccaccgt cagtacgtga
     8221 gatatcttta accctgatcc tggcaatttc ggctatacgt aacagggtgt tataagcaat
     8281 ccccagaaat gccagattac gtatatcctg gcagcgatcg ctattttcca tgagtgaacg
     8341 aacctggtcg aaatcagtgc gttcgaacgc tagagcctgt tttgcacgtt caccggcatc
     8401 aacgttttct tttcggatcc gccgcataac cagtgaaaca gcattgctgt cacttggtcg
     8461 tggcagcccg gaccgacgat gaagcatgtt tagctggccc aaatgttgct ggatagtttt
     8521 tactgccaga ccgcgcgcct gaagatatag aagataatcg cgaacatctt caggttctgc
     8581 gggaaaccat ttccggttat tcaacttgca ccatgccgcc cacgaccggc aaacggacag
     8641 aagcattttc caggtatgct cagaaaacgc ctggcgatcc ctgaacatgt ccatcaggtt
     8701 cttgcgaacc tcatcactcg ttgcatcgac cggtaacttt tagattaaca tttgtttaaa
     8761 tatgaaatta ggcaatgtaa catactgcag gcaaattttg gtgtacggta agtaaattgg
     8821 acatatgatt taacaaagcg actataagtc agaaagtgag aatgagattg aaataattaa
     8881 ttcaacttat tcaattgatg atatgccagg attcctcttc ctttatatat cttcaatgtt
     8941 tcgattaaca ggtccattat ccggttttta gtttcgctgc acattgccga atgacaatcg
     9001 gattccttta agctttgctt ttgccctgtt ttctctcccc agtaatcgtt gggtgactga
     9061 accatccagt agccgagagt tacgtttttc ctgatttaat aaatatccat atctatttgt
     9121 ctcgtggtgt tttacagcaa ctaaccgaaa ttactatttt gcatcatcca accataaaga
     9181 gacaacaata attctagaaa acatataaca agtgtttttt tctatttacg tctcatacgc
     9241 aaagtccact ttaccatgtt ttggaaactt gtacccaacc attcattttt cgattttgta
     9301 atgaggctct tttcttctaa caatcattga ggtcattatc atgtgtcctc acctattgtt
     9361 taaattgagg cttgcgctat tcccaaaatt gtgggtatta ttttcatata tcagtatctg
     9421 caatttcggt tcaactccgg aagctcggta cccaactgtg ggaatactca ggtatcgtaa
     9481 gatgcaagag ttcgaatctc ttagcaacca ttattttttt tctctcaaca taacgagaac
     9541 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
     9601 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
     9661 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
     9721 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
     9781 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
     9841 tatatatata tatttcaagg atataccatt gtaatgtctg cccctaagaa gatcgtcgtt
     9901 ttgccaggtg accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct
     9961 atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct
    10021 gctatcgatg ctacaggtgt tccacttcca gatgaggcgc tggaagcctc caagaaggct
    10081 gatgccgttt tgttaggtgc tgtgggtggt cctaaatggg gtaccggtag tgttagacct
    10141 gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt
    10201 aactttgcat ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt
    10261 actgacttcg ttgttgttag agaattagtg ggaggtattt actttggtaa gagaaaggaa
    10321 gacgatggtg atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga
    10381 atcacaagaa tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc
    10441 ttggataaag ctaatgtttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc
    10501 atcaagaacg aatttcctac attgaaagtt caacatcaat tgattgattc tgccgccatg
    10561 atcctagtta agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt
    10621 gatatcatct ccgatgaagc ctccgttatc ccaggctcct tgggtttgtt gccatctgcg
    10681 tccttggcct ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccatggt
    10741 tccgctccag atttgccaaa gaataaggtc aaccctatcg ccactatctt gtctgctgca
    10801 atgatgttga aattgtcatt gaacttgcct gaagaaggta aagccattga agatgcagtt
    10861 aaaaaggttt tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc
    10921 gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt
    10981 tttttgtgat atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa
    11041 ttacaaaatg gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt
    11101 atcaagaagg agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg
    11161 ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag gaggagggca
    11221 tcaca
//
