LOCUS       pET3d-SF-DCTN1-TS.xdna    8587 bp      DNA     circular   14.04.21
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Homologous recombination reaction between [pET3d] as a targe
            t sequence  and [FLASH_DCTN1_TS.xdna] as an inserted sequenc
            e.
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    4110..7967
                     /label=DCTN1
                     /SerialCloner_Color=&hD00009
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             7944..7961
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             7968..7991
                     /label=Strep tag II
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             8028..8051
                     /label=Strep tag II
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     4010..4029
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      1218..1846
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             407..1066
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        139..167
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4089..4109
                     /label=SplitFlAsH Tag
                     /SerialCloner_Color=&h00C700
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 
       61 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 
      121 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 
      181 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 
      241 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 
      301 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 
      361 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 
      421 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG 
      481 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 
      541 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 
      601 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 
      661 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 
      721 ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT 
      781 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 
      841 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 
      901 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 
      961 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 
     1021 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 
     1081 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 
     1141 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 
     1201 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 
     1261 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 
     1321 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 
     1381 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 
     1441 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 
     1501 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 
     1561 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 
     1621 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 
     1681 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 
     1741 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 
     1801 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 
     1861 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 
     1921 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 
     1981 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA 
     2041 TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC 
     2101 GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC 
     2161 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT 
     2221 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC 
     2281 CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA 
     2341 TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC 
     2401 TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG 
     2461 TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA 
     2521 CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC 
     2581 TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG 
     2641 TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA 
     2701 ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA 
     2761 AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC 
     2821 GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG 
     2881 TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA 
     2941 TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG 
     3001 TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC 
     3061 CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG 
     3121 ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT 
     3181 CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT 
     3241 TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG 
     3301 CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT 
     3361 AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC 
     3421 GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT 
     3481 GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT 
     3541 CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG 
     3601 TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG 
     3661 CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC 
     3721 CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC 
     3781 CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC 
     3841 TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC 
     3901 CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC 
     3961 GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAATT AATACGACTC 
     4021 ACTATAGGGA GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT TTAAGAAGGA 
     4081 GATATACCAT GGGAAGTTGC TGCGGCGCAC AGAGCAAGAG GCACGTGTAC AGCCGGACGC 
     4141 CCAGCGGCAG CAGGATGAGT GCGGAGGCAA GCGCCCGGCC CCTGCGGGTG GGCTCCCGTG 
     4201 TAGAGGTGAT TGGAAAAGGC CACCGAGGCA CTGTGGCCTA TGTTGGAGCC ACACTGTTTG 
     4261 CCACTGGCAA ATGGGTAGGC GTGATTCTGG ATGAAGCAAA GGGCAAAAAT GATGGAACTG 
     4321 TTCAAGGCAG GAAGTACTTC ACTTGTGATG AAGGGCATGG CATCTTTGTG CGCCAGTCCC 
     4381 AGATCCAGGT ATTTGAAGAT GGAGCAGATA CTACTTCCCC AGAGACACCT GATTCTTCTG 
     4441 CTTCAAAAGT CCTCAAAAGA GAGGGAACTG ATACAACTGC AAAGACTAGC AAACTGCGGG 
     4501 GACTGAAGCC TAAGAAGACA CCGACAGCCC GAAAGACCAC AACTCGGCGA CCCAAGCCCA 
     4561 CGCGCCCAGC CAGTACTGGG GTGGCTGGGG CCAGTAGCTC CCTGGGCCCC TCTGGCTCAG 
     4621 CGTCAGCAGG TGAGCTGAGC AGCAGTGAGC CCAGCACCCC GGCTCAGACT CCGCTGGCAG 
     4681 CACCCATCAT CCCCACGCCG GTCCTCACCT CTCCTGGAGC AGTCCCCCCG CTTCCTTCCC 
     4741 CATCCAAGGA GGAGGAGGGA CTAAGGGCTC AGGTGCGGGA CCTGGAGGAG AAACTAGAGA 
     4801 CCCTGAGACT GAAACGGGCA GAAGACAAAG CAAAGCTAAA AGAGCTGGAG AAACACAAAA 
     4861 TCCAGCTGGA GCAGGTGCAG GAATGGAAGA GCAAAATGCA GGAGCAGCAG GCCGACCTGC 
     4921 AGCGGCGCCT CAAGGAGGCG AGAAAGGAAG CCAAGGAGGC GCTGGAGGCA AAGGAACGCT 
     4981 ATATGGAGGA GATGGCTGAT ACTGCTGATG CCACTGAGAT GGCCACTTTG GACAAGGAGA 
     5041 TGGCTGAAGA GCGGGCTGAG TCCCTGCGGC AGGAGGTGGA GGCACTGAAG GAGCGGGTGG 
     5101 ACGAGCTCAC TACTGACTTA GAGATCCTCA AGGCTGAGAT TGAAGAGAAG GGCTCAGATG 
     5161 GCGCTGCATC CAGTTATCAG CTCAAGCAGC TTGAGGAGCA GAATGCCCGC CTGAAGGATG 
     5221 CCCTGGTGAG GATGCGGGAT CTTTCTTCCT CAGAGAAGCA GGAGCATGTG AAGCTCCAGA 
     5281 AGCTCATGGA AAAGAAGAAC CAAGAGCTGG AAGTTGTGAG GCAACAGCGG GAGCGTCTGC 
     5341 AGGAGGAGCT AAGCCAGGCA GAGAGCACCA TTGATGAGCT CAAGGAGCAG GTGGATGCTG 
     5401 CTCTGGGTGC TGAGGAGATG GTGGAGATGC TGACAGATCG GAACCTGAAT CTGGAAGAGA 
     5461 AAGTGCGCGA GTTGAGGGAG ACTGTGGGAG ACTTGGAAGC GATGAATGAG ATGAACGATG 
     5521 AGCTGCAGGA GAATGCACGT GAGACAGAAC TGGAGCTGCG GGAGCAGCTG GACATGGCAG 
     5581 GCGCGCGGGT TCGTGAGGCC CAGAAGCGTG TGGAGGCAGC CCAGGAGACG GTTGCAGACT 
     5641 ACCAGCAGGC CATCAAGAAG TACCGCCAGC TGACCGCCCA TCTACAGGAT GTGAATCGGG 
     5701 AACTGACAAA CCAGCAGGAA GCATCTGTGG AGAGGCAACA GCAGCCACCT CCAGAGACCT 
     5761 TTGACTTCAA AATCAAGTTT GCTGAGACTA AGGCTCATGC CAAGGCAATT GAGATGGAAT 
     5821 TGAGGCAGAT GGGGGTGGCC CAGGCCAATC GACACATGTC CCTGCTGACA GGCTTCATGC 
     5881 CTGACAGCTT CCTTCGGCCA GGTGGGGACC ATGACTGCGT TCTGGTGCTG TTGCTCATGC 
     5941 CTCGTCTCAT TTGCAAGGCA GAGCTGATCC GGAAGCAGGC CCAGGAGAAG TTTGAACTAA 
     6001 GTGAGAACTG TTCAGAGCGG CCTGGGCTGC GAGGAGCTGC TGGGGAGCAA CTCAGCTTTG 
     6061 CTGCTGGACT GGTGTACTCG CTGAGCCTGC TGCAGGCCAC GCTACACCGC TATGAGCATG 
     6121 CCCTCTCTCA GTGCAGTGTG GATGTGTATA AGAAAGTGGG CAGCCTGTAC CCTGAGATGA 
     6181 GTGCCCATGA GCGCTCCTTG GATTTCCTCA TTGAACTGCT GCACAAGGAT CAGCTGGATG 
     6241 AGACTGTCAA TGTGGAGCCT CTCACCAAGG CCATCAAGTA CTATCAGCAT CTGTACAGCA 
     6301 TCCACCTTGC CGAACAGCCT GAGGACTGTA CTATGCAGCT GGCTGACCAC ATTAAGTTCA 
     6361 CGCAGAGTGC TCTGGACTGC ATGAGTGTGG AGGTAGGACG GCTGCGTGCC TTCTTGCAGG 
     6421 GTGGGCAGGA GGCTACAGAT ATTGCCCTCC TGCTCCGGGA TCTGGAAACT TCATGCAGTG 
     6481 ACATCCGCCA GTTCTGCAAG AAGATCCGAA GGCGAATGCC AGGGACAGAT GCTCCTGGGA 
     6541 TCCCAGCTGC ACTGGCCTTT GGACCACAGG TATCTGACAC GCTCCTAGAC TGCAGGAAAC 
     6601 ACTTGACGTG GGTCGTGGCT GTGCTGCAGG AGGTGGCAGC TGCTGCTGCC CAGCTCATTG 
     6661 CCCCACTGGC AGAGAATGAG GGGCTACTTG TGGCTGCTCT GGAGGAACTG GCTTTCAAAG 
     6721 CAAGCGAGCA GATCTATGGG ACCCCCTCCA GCAGCCCCTA TGAGTGTCTG CGCCAGTCAT 
     6781 GCAACATCCT CATCAGTACC ATGAACAAGC TGGCCACAGC CATGCAGGAG GGGGAGTATG 
     6841 ATGCAGAGCG GCCCCCCAGC AAGCCTCCAC CGGTTGAACT GCGGGCTGCT GCCCTTCGTG 
     6901 CAGAGATCAC AGATGCTGAA GGCCTGGGTT TGAAGCTCGA AGATCGAGAG ACAGTTATTA 
     6961 AGGAGTTGAA GAAGTCACTC AAGATTAAGG GAGAGGAGCT AAGTGAGGCC AATGTGCGGC 
     7021 TGAGCCTCCT GGAGAAGAAG TTGGACAGTG CTGCCAAGGA TGCAGATGAG CGCATCGAGA 
     7081 AAGTCCAGAC TCGGCTGGAG GAGACCCAGG CACTGCTGCG AAAGAAGGAG AAAGGGTTTG 
     7141 AGGAGACAAT GGATGCACTC CAGGCTGACA TCGACCAGCT GGAGGCAGAG AAGGCAGAAC 
     7201 TAAAGCAGCG TCTGAACAGC CAGTCCAAAC GCACGATTGA GGGACTCCGG GGCCCTCCTC 
     7261 CTTCAGGCAT TGCTACTCTG GTCTCTGGCA TTGCTGGTGA AGAACAGCAG CGAGGAGCCA 
     7321 TCCCTGGGCA GGCTCCAGGG TCTGTGCCAG GCCCAGGGCT GGTGAAGGAC TCACCACTGC 
     7381 TGCTTCAGCA GATCTCTGCC ATGAGGCTGC ACATCTCCCA GCTCCAGCAT GAGAACAGCA 
     7441 TCCTCAAGGG AGCCCAGATG AAGGCATCCT TGGCATCCCT GCCCCCTCTG CATGTTGCAA 
     7501 AGCTATCCCA TGAGGGCCCT GGCAGTGAGT TACCAGCTGG AGCGCTGTAT CGTAAGACCA 
     7561 GCCAGCTGCT GGAGACATTG AATCAATTGA GCACACACAC GCACGTAGTA GACATCACTC 
     7621 GCACCAGCCC TGCTGCCAAG AGCCCGTCGG CCCAACTTAT GGAGCAAGTG GCTCAGCTTA 
     7681 AGTCCCTGAG TGACACCGTC GAGAAGCTCA AGGATGAGGT CCTCAAGGAG ACAGTATCTC 
     7741 AGCGCCCTGG AGCCACAGTA CCCACTGACT TTGCCACCTT CCCTTCATCA GCCTTCCTCA 
     7801 GGGCCAAGGA GGAGCAGCAG GATGACACAG TCTACATGGG CAAAGTGACC TTCTCATGTG 
     7861 CGGCTGGTTT TGGACAGCGA CACCGGCTGG TGCTGACCCA GGAGCAGCTG CACCAGCTTC 
     7921 ACAGTCGCCT CATCTCCggc gccCACCACC ACCACCACCA CggcgccTGG AGCCACCCGC 
     7981 AGTTCGAGAA AGGTGGAGGT TCCGGAGGTG GATCGGGAGG TTCGGCGTGG AGCCACCCGC 
     8041 AGTTCGAAAA AGGCGCCctt aagTAAACTA GTGGATCCGG CTGCTAACAA AGCCCGAAAG 
     8101 GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT 
     8161 AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC 
     8221 GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC 
     8281 TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT 
     8341 CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT 
     8401 ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT 
     8461 GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG 
     8521 TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC 
     8581 ATGAGAA
//