LOCUS       pMV306-LNKdi            4031 bp    DNA     circular     25-MAR-2021
FEATURES             Location/Qualifiers
     gene            780..1595
                     /note="KanR"
     rep_origin      1859..2259
                     /note="oriE"
     PCR_primer      2315..2334
                     /note="pMV361Seq_Fw2"
                     /current=0
     PCR_primer      complement(3977..3996)
                     /note="pMV361Seq_Rv3"
     PCR_primer      2616..2635
                     /note="pMV361Seq_Fw3"
     PCR_primer      complement(763..782)
                     /note="pMV361_AflII_Rv"
     PCR_primer      1596..1616
                     /note="pMV361_PacI_Fw"
     PCR_primer      complement(1862..1881)
                     /note="pMV306_Kan_Rv1"
     PCR_primer      complement(1798..1817)
                     /note="pMV306_Kan_Rv2"
     PCR_primer      complement(9..28)
                     /note="pMV306 seq Rv5"
     PCR_primer      complement(493..510)
                     /note="pMV306 delint BamHI Rv"
     PCR_primer      511..534
                     /note="pMV306 delint BamHI Fw"
     PCR_primer      2706..2724
                     /note="pLeft_XbaI_Fw"
     promoter        2706..3105
                     /note="pLeft *"
     RBS             3160..3165
                     /note="RBS1"
     misc_feature    3024..3029
                     /note="-35"
     misc_feature    3049..3053
                     /note="-10"
     PCR_primer      complement(3102..3114)
                     /note="pLeft_ClaI_Rv"
     gene            3216..3907
                     /note="mScarlet proper"
     PCR_primer      3216..3227
                     /note="mScarlet_NcoI_Fw"
     misc_feature    3248..3250
                     /note=""
     misc_feature    3266..3268
                     /note=""
     misc_feature    3278..3280
                     /note=""
     source          1..4031
                     /dnas_title="pMV306-LNKdi star-His6-mScarlet"
ORIGIN      
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag GGATCCtccc aaggacactg agtcctaaag
      541 aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg
      601 ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca
      661 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
      721 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      781 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      841 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      901 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      961 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     1021 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     1081 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1141 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1201 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     1261 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     1321 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1381 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1441 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     1501 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1561 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     1621 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1681 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1741 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1801 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1861 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2581 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2641 gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtaccag atctttaaat
     2701 ctagaCGATG ATAAGCGGTC AAACATGAGA ATTCGCGGCC GCATAATACG ACTCACTATA
     2761 GGGATCTTAA TTAAGGCGCC TCATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
     2821 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
     2881 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCAGATCT
     2941 GATATCGCTA GAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGCTA
     3001 GGGCACCAAT TTGCGATTAG GGCTTGACAG CCACCCGGCC AGTAGTGCAT TCTTGTGTCA
     3061 CCGCAGCAGC AAGGCGGTAG GCGGATCCGA GAGGATCGTG CCGGTGCCGG TGAAgagaga
     3121 ttcgccgccc gaaatgagca cgatccgcat gcttaattaa GGAGGTATCT CCATGAGCGG
     3181 TCACCATCAC CATCACCACG GCGGTAGCGC CATGGTGAGC AAGGGCGAGG CAGTGATCAA
     3241 GGAGTTCATG CGGTTCAAGG TGCACATGGA GGGCTCCATG AACGGCCACG AGTTCGAGAT
     3301 CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC
     3361 CAAGGGTGGC CCCCTGCCCT TCTCCTGGGA CATCCTGTCC CCTCAGTTCA TGTACGGCTC
     3421 CAGGGCCTTC ACCAAGCACC CCGCCGACAT CCCCGACTAC TATAAGCAGT CCTTCCCCGA
     3481 GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCGCCGTGA CCGTGACCCA
     3541 GGACACCTCC CTGGAGGACG GCACCCTGAT CTACAAGGTG AAGCTCCGCG GCACCAACTT
     3601 CCCTCCTGAC GGCCCCGTAA TGCAGAAGAA GACAATGGGC TGGGAAGCGT CCACCGAGCG
     3661 GTTGTACCCC GAGGACGGCG TGCTGAAGGG CGACATTAAG ATGGCCCTGC GCCTGAAGGA
     3721 CGGCGGCCGC TACCTGGCGG ACTTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGCAGAT
     3781 GCCCGGCGCC TACAACGTCG ACCGCAAGTT GGACATCACC TCCCACAACG AGGACTACAC
     3841 CGTGGTGGAA CAGTACGAAC GCTCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT
     3901 GTACAAGtga aagcttatcg atgtcgacgt agttaactag cgtacgatcg actgccaggc
     3961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatg ccatcatggc
     4021 cgcggtgatc a
//

