LOCUS       pTS2374-Tier1-PhCMV-Gal 5898 bp ds-DNA     circular     14-FEB-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    20..699
                     /label="Promoter Region"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    38..624
                     /label="P_CMV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    288..293
                     /label="REBASE:NdeI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        668..686
                     /label="T7 prom"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    739..1179
                     /label="Gal4 Binding Domain"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1198..1431
                     /label="VP16"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1498..1722
                     /label="BGH polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1768..2196
                     /label="f1 origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2201..2544
                     /label="SV40"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2606..3400
                     /label="NeoR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3574..3704
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4087..4757
                     /label="pUC ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(4902..5898)
                     /label="AmpR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gacggatcgg gagatctccA CGCGTGGTAC CCTCGAGttg acattgatta ttgagtagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      481 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      541 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      601 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      661 tcgaaattaa tacgactcac tatagggaga cccaagctgT TCGAAGCGGA ATTCACCATG
      721 ACTAGTggtg gttctggtAT GAAGCTACTG TCTTCTATCG AACAAGCATG CGATATTTGC
      781 CGACTTAAAA AGCTCAAGTG CTCCAAAGAA AAACCGAAGT GCGCCAAGTG TCTGAAGAAC
      841 AACTGGGAGT GTCGCTACTC TCCCAAAACC AAAAGGTCTC CGCTGACTAG GGCACATCTG
      901 ACAGAAGTGG AATCAAGGCT AGAAAGACTG GAACAGCTAT TTCTACTGAT TTTTCCTCGA
      961 GAAGACCTTG ACATGATTTT GAAAATGGAT TCTTTACAGG ATATAAAAGC ATTGTTAACA
     1021 GGATTATTTG TACAAGATAA TGTGAATAAA GATGCCGTCA CAGATAGATT GGCTTCAGTG
     1081 GAGACTGATA TGCCTCTAAC ATTGAGACAG CATAGAATAA GTGCGACATC ATCATCGGAA
     1141 GAGAGTAGTA ACAAAGGTCA AAGACAGTTG ACTGTATCGg CTAGTggtgg ttctggtgcc
     1201 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg
     1261 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
     1321 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc
     1381 gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg gGCTAGTgct
     1441 agcgGATCCA CCGGTgTCTA GAAAGCTTtg aGGCCGGcct gcagggatca gcctcgactg
     1501 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     1561 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     1621 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     1681 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     1741 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     1801 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1861 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1921 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1981 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2041 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2101 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2161 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     2221 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2281 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2341 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     2401 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     2461 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     2521 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     2581 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     2641 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     2701 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     2761 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     2821 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     2881 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     2941 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3001 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     3061 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     3121 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     3181 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3241 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3301 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3361 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     3421 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     3481 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     3541 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     3601 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3661 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     3721 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3781 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3841 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3901 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3961 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5881 aaagtgccac ctgacgtc
//