LOCUS       Exported                2985 bp ds-DNA     circular SYN 25-MAR-2021
DEFINITION  Host strain: CC118 Lambda pir.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Uzzau S, Figueroa-Bossi N, Rubino S, Bossi L.
  TITLE     Epitope tagging of chromosomal genes in Salmonella.
  JOURNAL   Proc Natl Acad Sci U S A 2001 Dec 18;98:15264-9
  PUBMED    11742086
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Lionello Bossi
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 29, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.261348198"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2001-12-18"; volume: "98"; pages: "15264-9"
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     CDS             133..993
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=ßla (AmpR)
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1492..1848
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1913..1936
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     1913..1933
                     /label=Fw primer annealing seq
     protein_bind    complement(1962..1995)
                     /label=FRT-1
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated recombination"
     CDS             complement(2026..2685)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat (CamR)
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    complement(2894..2927)
                     /label=FRT-2
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated recombination"
     primer_bind     complement(2938..2957)
                     /label=Rv primer annealing seq
ORIGIN
        1 gcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
       61 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      121 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      181 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      241 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      301 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      361 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      421 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      481 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      541 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      601 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      661 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      721 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      781 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      841 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      901 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      961 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1021 ctttagattg atttaaaact tcatttttaa ttttggtgca ctctcagtac aatctgctct
     1081 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     1141 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1201 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     1261 catcaccgaa acgcgcgagg cagcaaggag atggcgccca acagtccccc ggccacgggg
     1321 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     1381 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     1441 ccggccacga tgcgtccggc gtagaggatc cagcagttca acctgttgat agtacgtact
     1501 aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa
     1561 cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat
     1621 gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa
     1681 tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt
     1741 ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga
     1801 gcctctcaaa gcaattttca gtgacacagg aacacttaac ggctgacatg gccttagaag
     1861 gcgttttaga agccctaatg gatggagtca aaacaaacta gcgatcgaat tcgactacaa
     1921 agatgacgac gataaatagt aatgtaggct ggagctgctt cgaagttcct atactttcta
     1981 gagaatagga acttcggaat aggaacttca tttaaatggc gcgccttacg ccccgccctg
     2041 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2101 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2161 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2221 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     2281 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2341 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2401 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2461 catacgtaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2521 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2581 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2641 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2701 tcctgaaaat ctcgacaact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2761 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     2821 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     2881 aggtaggcgc gccgaagttc ctatactttc tagagaatag gaacttcgga ataggaacta
     2941 aggaggatat tcatatgcgg aattcccggg agagctcgat gatct
//
