LOCUS       Exported                6844 bp ds-DNA     circular SYN 30-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 30, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..190
                     /label=Inverted terminal repeat_ITR
                     /note="vntifkey=""19"" - label=ITR - note=""Inverted 
                     terminal repeat"" - 1..190"
     LTR             4..185
                     /label=Inverted Repeat_ITR
                     /note="vntifkey=""19"" - label=ITR - note=""Inverted 
                     Repeat"" - complement(3535..3716)"
     promoter        191..541
                     /label=U6 Promoter for shRNA transcription_U6\Promoter
                     /note="vntifkey=""29"" - label=U6\Promoter - note=""U6 
                     Promoter for shRNA transcription"" - 191..541"
     misc_feature    542..621
                     /label=MeCP2 shRNA, mouse, Zhou et al., Neuron 2006 ?_...
                     /note="vntifkey=""21"" - label=MeCP2\shRNA\(Zhou) - 
                     note=""MeCP2 shRNA, mouse, Zhou et al., Neuron 2006 ?"" - 
                     542..621"
     promoter        677..1530
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*]
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1532..1569
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*]
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1571..1575
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*] [*]
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1577..1588
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*] [...
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1590..1636
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*] [...
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1638..1651
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*] [...
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1653..1805
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*] [...
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1807..1812
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*]
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1814..1821
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*]
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1823..1873
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*] [*]
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     promoter        1877
                     /label=alpha CaMKII promoter_CaMKII\Promoter [*] [*] [...
                     /note="vntifkey=""29"" - label=CaMKII\Promoter - 
                     note=""alpha CaMKII promoter"" - 677..1877"
     CDS             1898..2117
                     /label=mCherry_mCherry [*]
                     /note="vntifkey=""4"" - label=mCherry - note=""mCherry"" - 
                     1898..2608"
     CDS             2119..2608
                     /label=mCherry_mCherry [*]
                     /note="vntifkey=""4"" - label=mCherry - note=""mCherry"" - 
                     1898..2608"
     polyA_signal    2650..3533
                     /label=WPRE and bGH poly A site_WPRE\&\bGH\poly\A
                     /note="vntifkey=""25"" - label=WPRE\"
     misc_feature    complement(2743..2760)
                     /label=WPRE_Seq
     CDS             complement(3127..3138)
                     /label=FactorXa site
     terminator      3274..3501
                     /label=bGH PA
     promoter        complement(3722..3918)
                     /label=SV40 early promoter
     misc_feature    complement(3794..3871)
                     /label=SV40 ORI
     rep_origin      complement(4279..4907)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(5056..5916)
                     /label=Amp\R
                     /note="vntifkey=""4"" - label=Amp\R - 
                     complement(5056..5916)"
     CDS             complement(5059..5718)
                     /label=AmpR
     promoter        complement(5958..5986)
                     /label=Amp prom
     primer_bind     6809..6826
                     /label=SP6
ORIGIN
        1 tagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggtacc ctcgaggagg agaagcatga attccccagt ggaaagacgc gcaggcaaaa
      241 cgcaccacgt gacggagcgt gaccgcgcgc cgagcgcgcg ccaaggtcgg gcaggaagag
      301 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      361 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      421 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      481 cttaccgtaa cttgaaagta tttcgatttc ttgggtttat atatcttgtg gaaaggacgc
      541 gggatcccgt cagaagacca ggatctccat tcaagagatg gagatcctgg tcttctgact
      601 tttttggaaa gatctaagct tggcactggc cgtactagaa aatgagatgg gggtgcagag
      661 agcttcctca gtgacctgcc cagggtcaca tcagaaatgt cagagctaga acttgaactc
      721 agattactaa tcttaaattc catgccttgg gggcatgcaa gtacgatata cagaaggagt
      781 gaactcatta gggcagatga ccaatgagtt taggaaagaa gagtccaggg cagggtacat
      841 ctacaccacc cgcccagccc tgggtgagtc cagccacgtt cacctcatta tagttgcctc
      901 tctccagtcc taccttgacg ggaagcacaa gcagaaactg ggacaggagc cccaggagac
      961 caaatcttca tggtccctct gggaggatgg gtggggagag ctgtggcaga ggcctcagga
     1021 ggggccctgc tgctcagtgg tgacagatag gggtgagaaa gcagacagag tcattccgtc
     1081 agcattctgg gtctgtttgg tacttcttct cacgctaagg tggcggtgtg atatgcacaa
     1141 tggctaaaaa gcagggagag ctggaaagaa acaaggacag agacagaggc caagtcaacc
     1201 agaccaattc ccagaggaag caaagaaacc attacagaga ctacaagggg gaagggaagg
     1261 agagatgaat tagcttcccc tgtaaacctt agaacccagc tgttgccagg gcaacggggc
     1321 aatacctgtc tcttcagagg agatgaagtt gccagggtaa ctacatcctg tctttctcaa
     1381 ggaccatccc agaatgtggc acccactagc cgttaccata gcaactgcct ctttgcccca
     1441 cttaatccca tcccgtctgt taaaagggcc ctatagttgg aggtggggga ggtaggaaga
     1501 gcgatgatca cttgtggact aagtttgttc gcatcccctt ctccaacccc ctcagtacat
     1561 caccctgggg gaacagggtc cacttgctcc tgggcccaca cagtcctgca gtattgtgta
     1621 tataaggcca gggcaaagag gagcaggttt taaagtgaaa ggcaggcagg tgttggggag
     1681 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
     1741 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
     1801 cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag
     1861 ttctgggggc agcgggggat caggtggcag cggtggcatg gtgagcaagg gcgaggagga
     1921 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1981 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2041 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     2101 tcagttcatg tacggcttca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2161 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2221 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2281 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     2341 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     2401 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     2461 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     2521 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     2581 cggcggcatg gacgagctgt acaagtaggg tggcagcggt ggcactcgag taagctatca
     2641 agctagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3061 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3181 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3241 ccgcatcgat accgtcgact cgctgatcag cctcgactgt gccttctagt tgccagccat
     3301 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3361 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3421 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3481 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgactagag
     3541 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     3601 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     3661 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagagct
     3721 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     3781 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     3841 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     3901 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     3961 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     4021 ctggggactt tccacaccct aactgacaca cattccacag ctgcattaat gaatcggcca
     4081 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     4141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4921 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4981 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5041 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5101 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5161 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5221 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5281 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5341 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5401 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5461 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5521 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5581 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5641 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5701 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5761 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5821 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5881 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5941 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6001 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     6061 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     6121 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6181 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     6241 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     6301 ttcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     6361 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     6421 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     6481 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     6541 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctggctagcg atgaccctgc
     6601 tgattggttc gctgaccatt tccgggtgcg ggacggcgtt accagaaact cagaaggttc
     6661 gtccaaccaa accgactctg acggcagttt acgagagaga tgatagggtc tgcttcagta
     6721 agccagatgc tacacaatta ggcttgtaca tattgtcgtt agaacgcggc tacaattaat
     6781 acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaata cacggaatta
     6841 attc
//
