LOCUS       HY_8.F                  7259 bp    DNA     circular UNA 26-AUG-2018
DEFINITION  pcD5.tetO2_FF4_EGFP_miRNA_FF4.
ACCESSION   urn.local...4k-9iecj8r
VERSION     urn.local...4k-9iecj8r
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
FEATURES             Location/Qualifiers
     misc_feature    12..17
                     /Recognition_pattern="A^GATCT"
                     /annotation_group="AGATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     promoter        236..812
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="CAG_enhancer"
     misc_feature    483..488
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     misc_feature    610..615
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     primer_bind     731..748
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="511731570928364009ba626-e9bd-418e-aa8e-
                     e230dbcb24e5"
                     /%GC="44.4"
                     /Tm="51.4"
                     /Hairpin_Tm="39.3"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGACTTTCCAAAATGTCG"
                     /label="V1-5"
     primer_bind     745..762
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="5117310314335821ecaf71e-f333-4c32-b420-
                     4be8c68131d4"
                     /%GC="61.1"
                     /Tm="58.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTCGTAACAACTCCGCCC"
                     /label="EGFPN1F"
     misc_feature    765..770
                     /created_by="User"
                     /label="cmv 35box"
     primer_bind     769..789
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="511730877937418cf8d1c17-7c20-4546-910f-
                     a2ee9a2d7e1c"
                     /%GC="66.7"
                     /Tm="67.0"
                     /Hairpin_Tm="52.4"
                     /Self_Dimer_Tm="3.5"
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /label="CMVf"
     misc_feature    769..789
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="CMV_fwd_primer"
     misc_feature    804..810
                     /created_by="User"
                     /label="TATA_box"
     misc_feature    814..832
                     /created_by="User"
                     /label="TetO2"
     misc_feature    833..839
                     /created_by="User"
                     /label="Initiator Motif"
     misc_feature    835..840
                     /Recognition_pattern="A^GATCT"
                     /annotation_group="AGATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     misc_feature    840..858
                     /created_by="User"
                     /label="TetO2"
     misc_feature    901..906
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="Kpn2I"
                     /note="Geneious type: restriction site"
     primer_bind     927..943
                     /Sequence="CTGGGTACCCGGGGATC"
                     /Hairpin_Tm="58.1"
                     /Tm="58.0"
                     /Self_Dimer_Tm="21.0"
                     /%GC="70.6"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="3127.pcD5_BamH1.for"
     misc_feature    929
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    930..935
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    934..939
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    939..944
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    1015..1020
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="Kpn2I"
                     /note="Geneious type: restriction site"
     misc_feature    1041..1613
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="bGlob_int"
     misc_feature    1157..1162
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    1622..1643
                     /created_by="User"
                     /modified_by="User"
                     /label="FF4"
     misc_feature    complement(1634..1668)
                     /Sequence="CGCCCTTGCTCACCATGGTGGCGCCTTTAATTAAA"
                     /Hairpin_Tm="45.4"
                     /Tm="73.3"
                     /Self_Dimer_Tm="31.4"
                     /%GC="51.4"
                     /created_by="primer3"
                     /label="3131.FF4_Kozac.rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1644..>1652
                     /created_by="User"
                     /modified_by="User"
                     /label="Kozac"
     misc_feature    1651..1656
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    1652^1653
                     /Original_Bases="ATGGAGTCTAGACTGGACAAGAGCAAAGTCATAAACTCTGC
                     TCTGGAATTACTCAATGAAGTCGGTATCGAAGGCCTGACGACAAGGAAACTCGCTCAA
                     AAGCTGGGAGTTGAGCAGCCTACCCTGTACTGGCACGTGAAGAACAAGCGGGCCCTGC
                     TCGATGCCCTGGCAATCGAGATGCTGGACAGGCATCATACCCACTTCTGCCCCCTGGA
                     AGGCGAGTCATGGCAAGACTTTCTGCGGAACAACGCCAAGTCATTCCGCTGTGCTCTC
                     CTCTCACATCGCGACGGGGCTAAAGTGCATCTCGGCACCCGCCCAACAGAGAAACAGT
                     ACGAAACCCTGGAAAATCAGCTCGCGTTCCTGTGTCAGCAAGGCTTCTCCCTGGAGAA
                     CGCACTGTACGCTCTGTCCGCCGTGGGCCACTTTACACTGGGCTGCGTATTGGAGGAT
                     CAGGAGCATCAAGTAGCAAAAGAGGAAAGAGAGACACCTACCACCGATTCTATGCCCC
                     CACTTCTGAGACAAGCAATTGAGCTGTTCGACCATCAGGGAGCCGAACCTGCCTTCCT
                     TTTCGGCCTGGAACTAATCATATGTGGCCTGGAGAAACAGCTAAAGTGCGAAAGCGGC
                     GGGCCAAAAAAGAAGAGAAAGGGTGACGGTGCTGGTTTAATTAACATGGGAAGCGGAG
                     CTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTGC
                     TAGC"
                     /label="ATGGAGTCTAGACTGGACAAGAGCAAAGTCATAAACTCTGCTCTGGAATT
                     ACTCAATGAAGTCGGTATCGAAGGCCTGACGACAAGGAAACTCGCTCAAAAGCTGGGA
                     GTTGAGCAGCCTACCCTGTACTGGCACGTGAAGAACAAGCGGGCCCTGCTCGATGCCC
                     TGGCAATCGAGATGCTGGACAGGCATCATACCCACTTCTGCCCCCTGGAAGGCGAGTC
                     ATGGCAAGACTTTCTGCGGAACAACGCCAAGTCATTCCGCTGTGCTCTCCTCTCACAT
                     CGCGACGGGGCTAAAGTGCATCTCGGCACCCGCCCAACAGAGAAACAGTACGAAACCC
                     TGGAAAATCAGCTCGCGTTCCTGTGTCAGCAAGGCTTCTCCCTGGAGAACGCACTGTA
                     CGCTCTGTCCGCCGTGGGCCACTTTACACTGGGCTGCGTATTGGAGGATCAGGAGCAT
                     CAAGTAGCAAAAGAGGAAAGAGAGACACCTACCACCGATTCTATGCCCCCACTTCTGA
                     GACAAGCAATTGAGCTGTTCGACCATCAGGGAGCCGAACCTGCCTTCCTTTTCGGCCT
                     GGAACTAATCATATGTGGCCTGGAGAAACAGCTAAAGTGCGAAAGCGGCGGGCCAAAA
                     AAGAAGAGAAAGGGTGACGGTGCTGGTTTAATTAACATGGGAAGCGGAGCTACTAACT
                     TCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTGCTAGC"
                     /note="Geneious type: Editing History Deletion"
     source          order(<1653..>2369,<1653..>2369)
                     /organism="pRS416"
                     /mol_type="other DNA"
                     /label="source pRS416"
     CDS             1653..2372
                     /modified_by="User"
                     /label="EGFP"
     primer_bind     1653..1671
                     /Sequence="ATGGTGAGCAAGGGCGAGG"
                     /Hairpin_Tm="47.5"
                     /Tm="62.3"
                     /Self_Dimer_Tm="None"
                     /%GC="63.2"
                     /created_by="primer3"
                     /label="2205.for"
     misc_feature    <1653..1658
                     /label="misc"
     misc_feature    1656..2776
                     /Original_Bases="TCGCGGAGGCCATGGCTTCTTGCCCTAGCGCTGGCAGTGGC
                     GCTGGCGGCCGGCAGCGCAGGAGCCTCGACTGGCAGTGACGCGACGGTGCCGGTCGCG
                     ACTCAGGATGGCCCCGACTACGTTTTCCACCGTGCCCACGAGCGCATGCTCTTCCAAA
                     CCTCATACACTCTTGAGAACAATGGTTCTGTTATTTGCATCCCGAACAACGGCCAGTG
                     CTTCTGCTTGGCTTGGCTTAAATCCAACGGAACAAATGCCGAGAAGTTGGCTGCCAAC
                     ATTCTGCAGTGGATTACTTTTGCGCTTTCAGCGCTCTGCCTGATGTTCTACGGCTACC
                     AGACCTGGAAGTCTACTTGCGGCTGGGAGGAGATTTACGTGGCCACGATCGAGATGAT
                     CAAGTTCATCATCGAGTATTTCCATGAGTTTGACGAACCTGCGGTGATCTACTCATCC
                     AACGGCAACAAGACCGTGTGGCTTCGTTACGCGGAGTGGCTGCTGACCTGCCCTGTCA
                     TTCTTATCCATCTGAGCAACCTTACGGGTCTGGCGAACGACTATAACAAGCGTACCAT
                     GGGTCTGCTGGTGTCAGATATCGGCACGATCGTGTGGGGCACCACGGCCGCGCTGTCC
                     AAGGGATACGTCCGTGTCATTTTCTTCCTGATGGGCCTGTGCTACGGCATCTACACAT
                     TCTTCAACGCAGCCAAGGTCTACATTGAGGCGTACCACACCGTGCCCAAGGGCATTTG
                     CCGCGACCTGGTCCGCTACCTTGCCTGGCTCTACTTCTGTTCATGGGCTATGTTCCCG
                     GTGCTGTTCCTGCTGGGCCCCGAGGGCTTTGGCCACATCAACCAATTCAACTCTGCCA
                     TCGCCCACGCCATCCTGGACCTTGCCTCCAAGAACGCTTGGAGTATGATGGGTCACTT
                     TCTGCGTGTCAAGATCCACGAGCACATCCTGCTGTACGGCGACATCCGCAAGAAGCAG
                     AAGGTCAACGTGGCTGGCCAGGAGATGGAGGTGGAGACCATGGTGCACGAGGAGGACG
                     ACGAGGCGGCCGCCGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCT
                     GGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAG
                     GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGC
                     CCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCG
                     CTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTAC
                     GTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG
                     TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAA
                     GGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTC
                     TATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACA
                     ACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGG
                     CGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGC
                     AAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCG
                     GGATCACTCTCGGCATGGACGAGCTGTAATGA"
                     /label="TCGCGGAGGCCATGGCTTCTTGCCCTAGCGCTGGCAGTGGCGCTGGCGGC
                     CGGCAGCGCAGGAGCCTCGACTGGCAGTGACGCGACGGTGCCGGTCGCGACTCAGGAT
                     GGCCCCGACTACGTTTTCCACCGTGCCCACGAGCGCATGCTCTTCCAAACCTCATACA
                     CTCTTGAGAACAATGGTTCTGTTATTTGCATCCCGAACAACGGCCAGTGCTTCTGCTT
                     GGCTTGGCTTAAATCCAACGGAACAAATGCCGAGAAGTTGGCTGCCAACATTCTGCAG
                     TGGATTACTTTTGCGCTTTCAGCGCTCTGCCTGATGTTCTACGGCTACCAGACCTGGA
                     AGTCTACTTGCGGCTGGGAGGAGATTTACGTGGCCACGATCGAGATGATCAAGTTCAT
                     CATCGAGTATTTCCATGAGTTTGACGAACCTGCGGTGATCTACTCATCCAACGGCAAC
                     AAGACCGTGTGGCTTCGTTACGCGGAGTGGCTGCTGACCTGCCCTGTCATTCTTATCC
                     ATCTGAGCAACCTTACGGGTCTGGCGAACGACTATAACAAGCGTACCATGGGTCTGCT
                     GGTGTCAGATATCGGCACGATCGTGTGGGGCACCACGGCCGCGCTGTCCAAGGGATAC
                     GTCCGTGTCATTTTCTTCCTGATGGGCCTGTGCTACGGCATCTACACATTCTTCAACG
                     CAGCCAAGGTCTACATTGAGGCGTACCACACCGTGCCCAAGGGCATTTGCCGCGACCT
                     GGTCCGCTACCTTGCCTGGCTCTACTTCTGTTCATGGGCTATGTTCCCGGTGCTGTTC
                     CTGCTGGGCCCCGAGGGCTTTGGCCACATCAACCAATTCAACTCTGCCATCGCCCACG
                     CCATCCTGGACCTTGCCTCCAAGAACGCTTGGAGTATGATGGGTCACTTTCTGCGTGT
                     CAAGATCCACGAGCACATCCTGCTGTACGGCGACATCCGCAAGAAGCAGAAGGTCAAC
                     GTGGCTGGCCAGGAGATGGAGGTGGAGACCATGGTGCACGAGGAGGACGACGAGGCGG
                     CCGCCGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCT
                     GGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCC
                     ACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCT
                     GGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGA
                     CCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
                     CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCG
                     AGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGG
                     CAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATG
                     GCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGG
                     ACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCC
                     CGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCC
                     AACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC
                     TCGGCATGGACGAGCTGTAATGA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(1694..1712)
                     /modified_by="User"
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="21632780836460948c60bf4-8121-4b03-80d6-
                     4ffaa008605b"
                     /%GC="63.2"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTCCAGCTCGACCAGGATG"
                     /label="EGFPN1R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2339..2357
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="216327575511730bb624243-6d42-4101-8a89-
                     f0ffedcd5e8f"
                     /%GC="57.9"
                     /Tm="57.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GATCACTCTCGGCATGGAC"
                     /label="EGFPC1F"
     misc_feature    2373..2394
                     /created_by="User"
                     /modified_by="User"
                     /label="FF4"
     misc_feature    2395..2400
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    2401..2776
                     /modified_by="User"
                     /label="miRNA FF4"
     misc_feature    2530..2535
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2535..2593)
                     /created_by="User"
                     /Sequence="ACATCTGTGGCTTCACTATTTAATTAAAGACTTCAAGCGGCGCTCAC
                     TGTCAACAGCAC"
                     /modified_by="User"
                     /label="2945.miRNA_FF4.rev"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2579..2637
                     /created_by="User"
                     /Sequence="TGAAGCCACAGATGTATTTAATTAAAGACTTCAAGCGGTGCCTACTG
                     CCTCGGAGAATT"
                     /modified_by="User"
                     /label="2946.miRNA_FF4.for"
     misc_feature    2633..2638
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2747..2794)
                     /created_by="User"
                     /Sequence="GAGGTTGATTGCGGCCGCCTGAGGAAAAAAAAGGAAACAATTGAAAA
                     A"
                     /modified_by="User"
                     /label="3130.miRNA_FF3_Not1.rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2777..2784
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    3174..3179
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    3180..3185
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3211..3228)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="511730800114877e7ee8e53-1158-4a67-be82-
                     fa985c260378"
                     /%GC="55.6"
                     /Tm="55.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /label="BGHrev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3211..3228)
                     /Transferred_Translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLA
                     QKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
                     LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE
                     DQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCES
                     GGPKKKRKGDGAGLINMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="BGH_rev_primer"
     terminator      3214..3441
                     /label="bGH_PA_terminator"
     misc_feature    complement(3215..3234)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="51173133933710042961fab-f157-4b95-bc31-
                     007569234302"
                     /%GC="55.0"
                     /Tm="58.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.6"
                     /Sequence="GGCAACTAGAAGGCACAGTC"
                     /label="pcDNAR"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      3504..3789
                     /label="f1_origin"
     misc_feature    3752..3757
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    3998..4003
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="Kpn2I"
                     /note="Geneious type: restriction site"
     misc_feature    4020..4025
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    4110..4115
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    4129..4134
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    4226..4231
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     misc_feature    4535..4540
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="Kpn2I"
                     /note="Geneious type: restriction site"
     misc_feature    4671..4676
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="Kpn2I"
                     /note="Geneious type: restriction site"
     terminator      4935..5054
                     /Transferred_Translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLA
                     QKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
                     LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE
                     DQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCES
                     GGPKKKRKGDGAGLINMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="SV40_PA_terminator"
     misc_feature    complement(4969..4988)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="51173149513119652a678c2-4d19-49f3-b7fe-
                     b15a53f8a8f5"
                     /%GC="35.0"
                     /Tm="51.5"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAAATTTGTGATGCTATTGC"
                     /label="SV40pA-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5023..5042
                     /Transferred_Translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLA
                     QKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
                     LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE
                     DQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCES
                     GGPKKKRKGDGAGLINMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="EBV_rev_primer"
     misc_feature    5069..5074
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     promoter        complement(5098..5116)
                     /Transferred_Translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLA
                     QKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
                     LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE
                     DQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCES
                     GGPKKKRKGDGAGLINMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="M13_reverse_primer"
     misc_feature    complement(5101..5118)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="51173118430594045c22f80-73a9-4fcc-837e-
                     1c44d8ce2adf"
                     /%GC="50.0"
                     /Tm="52.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGGAAACAGCTATGACC"
                     /label="M13R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(5115..5138)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="511731260265427abd41cd0-3181-4c49-9b5b-
                     77a0d735c417"
                     /%GC="45.8"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /label="M13R49"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(5115..5137)
                     /Transferred_Translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLA
                     QKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
                     LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE
                     DQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCES
                     GGPKKKRKGDGAGLINMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="M13_pUC_rev_primer"
     promoter        complement(5151..5180)
                     /Transferred_Translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLA
                     QKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
                     LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE
                     DQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCES
                     GGPKKKRKGDGAGLINMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="lac_promoter"
     misc_feature    complement(5160..5185)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="511731418897251a5140f2c-97db-4b1d-80b8-
                     dc64b294e963"
                     /%GC="50.0"
                     /Tm="64.7"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGGCTTTACACTTTATGCTTCCGGC"
                     /label="pexR"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(5385..5404)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="51173164656321398176229-db96-4b69-84df-
                     02eb476e7824"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /label="VR"
                     /note="Geneious type: primer_bind_reverse"
     gene            complement(6263..7123)
                     /gene="Ampicillin"
                     /Transferred_Translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLA
                     QKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
                     LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE
                     DQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCES
                     GGPKKKRKGDGAGLINMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
                     KNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITLGMDELYK*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="Ampicillin"
     cds             complement(6263..7123)
                     /Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS
                     QATMDERNRQIAEIGASLIKHW*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="ORF frame 2"
     promoter        complement(7165..7193)
                     /Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS
                     QATMDERNRQIAEIGASLIKHW*"
                     /Transferred_From="pDN-D2irTNG4kwh"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR_promoter"
     misc_feature    7257..3
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcatccctat cagtgataga gatcagatct
      841 ccctatcagt gatagagagc tgttttgacc tccatagaag acaccgggac cgatccagcc
      901 tccggactct agcgtttaaa cttaagctgg gtacccgggg atcctctagg gcctctgagc
      961 tattccagaa gtagtgaaga ggcttttttg gaggcctagg cttttgcaaa aagctccgga
     1021 tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     1081 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     1141 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     1201 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     1261 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     1321 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     1381 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     1441 ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc
     1501 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     1561 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta caggtcctgc
     1621 accgcttgaa gtctttaatt aaaggcgcca ccatggtgag caagggcgag gagctgttca
     1681 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1741 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1801 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     1861 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1921 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1981 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2041 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2101 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2161 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2221 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2281 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2341 tcactctcgg catggacgag ctgtacaagt agccgcttga agtctttaat taaaaagctt
     2401 gtgagtatgt gctcgcttcg gcagcacata tactatgtcg aatgaggctt cagtacttta
     2461 cagaatcgtt gcctgcacat cttggaaaca cttgctggga ttacttcttc aggttaaccc
     2521 aacagaaggc tcgagtgctg ttgacagtga gcgccgcttg aagtctttaa ttaaatagtg
     2581 aagccacaga tgtatttaat taaagacttc aagcggtgcc tactgcctcg gagaattcaa
     2641 ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc tatctctttg
     2701 atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt tcaattgttt
     2761 cctttttttt cctcaggcgg ccgcaatcaa cctctggatt acaaaatttg tgaaagattg
     2821 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2881 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2941 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3001 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3061 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3121 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcctcgagt
     3181 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     3241 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3301 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3361 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3421 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     3481 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3541 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3601 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3661 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3721 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     3781 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     3841 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     3901 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     3961 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     4021 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     4081 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     4141 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     4201 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     4261 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     4321 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     4381 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     4441 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     4501 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     4561 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     4621 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     4681 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     4741 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     4801 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4861 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4921 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5041 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5101 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5161 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5221 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5281 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5341 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5401 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5461 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5521 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5581 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5641 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5701 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5761 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5821 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5881 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5941 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6001 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6061 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6121 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6181 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6241 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6301 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6361 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6421 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6481 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6541 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6601 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6661 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6721 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6781 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6841 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6901 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6961 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7021 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
