LOCUS       pGRNA3                  3501 bp    DNA     circular UNA 30-MAR-2021
DEFINITION  .
ACCESSION   urn.local...u-d6s1vfx
VERSION     urn.local...u-d6s1vfx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    248
                     /Original_Bases="TTCGTT"
                     /label="TTCGTT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    250..254
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     tRNA            255..331
                     /created_by="User"
                     /label="tRNA"
     misc_feature    354..>429
                     /modified_by="User"
                     /label="sgRNA backbone"
     misc_feature    431..437
                     /Original_Bases="GCTGGCT"
                     /label="GCTGGCT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    855
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             889..1683
                     /created_by="sang-gyu"
                     /label="KanR"
     misc_feature    1071
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     CDS             1701..2561
                     /created_by="sang-gyu"
                     /label="AmpR"
     misc_feature    2417
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2420
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttc
      241 acctgcggct ggctaacaaa gcaccagtgg tctagtggta gaatagtacc ctgccacggt
      301 acagacccgg gttcgattcc cggctggtgc aagagacctc cggagtggtc tcagttttag
      361 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      421 agtcggtgcg aaggaaggca ggtgctcgag gggcccgtta tcggggaaga agtggctgat
      481 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
      541 atgtcaggca tgagattatc aaaaaggatc ttcacctaga tccttttcac gtagaaagcc
      601 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
      661 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
      721 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
      781 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
      841 cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
      901 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      961 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     1021 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     1081 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     1141 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     1201 tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     1261 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     1321 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     1381 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     1441 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1501 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1561 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1621 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1681 tgaattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1741 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1801 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1861 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1921 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1981 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2041 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2101 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2161 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2221 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2281 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2341 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2401 gagccggtga gcgtggttcg cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2461 cccgtatcgt agttatctac acgacgggga gccaggcaac tatggatgaa cgaaatagac
     2521 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2581 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2641 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2701 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2761 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2821 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2881 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2941 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3001 ggttggattc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3061 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3121 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3181 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3241 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3301 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3361 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3421 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgaat
     3481 cagtgagcga ggaagcggaa g
//
