LOCUS       pGRNA3e                 3425 bp    DNA     circular UNA 30-MAR-2021
DEFINITION  .
ACCESSION   urn.local...v-d6s1vfx
VERSION     urn.local...v-d6s1vfx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    248
                     /Original_Bases="TTCGTT"
                     /label="TTCGTT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    250..254
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     tRNA            255..331
                     /created_by="User"
                     /label="tRNA"
     misc_feature    354..>357
                     /modified_by="User"
                     /label="sgRNA backbone"
     misc_feature    357^358
                     /Original_Bases="TAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTA
                     TCAACTTGAAAAAGTGGCACCGAGTCGGTGC"
                     /label="TAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGA
                     AAAAGTGGCACCGAGTCGGTGC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    359..361
                     /Original_Bases="GCTGGCT"
                     /label="GCTGGCT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    779
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             813..1607
                     /created_by="sang-gyu"
                     /label="KanR"
     misc_feature    995
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     CDS             1625..2485
                     /created_by="sang-gyu"
                     /label="AmpR"
     misc_feature    2341
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2344
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttc
      241 acctgcggct ggctaacaaa gcaccagtgg tctagtggta gaatagtacc ctgccacggt
      301 acagacccgg gttcgattcc cggctggtgc aagagacctc cggagtggtc tcagtttgaa
      361 agcaggtgct cgaggggccc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
      421 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggcatgagat
      481 tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt
      541 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
      601 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
      661 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
      721 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagct
      781 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
      841 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
      901 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
      961 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     1021 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     1081 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatcccac cttgctcctg
     1141 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     1201 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     1261 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     1321 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     1381 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     1441 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     1501 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     1561 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt gaaaaaggaa
     1621 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1681 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1741 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1801 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1861 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1921 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1981 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2041 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2101 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2161 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2221 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2281 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2341 ttcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2401 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2461 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2521 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2581 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2761 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg attcaagacg
     2941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3361 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gaatcagtga gcgaggaagc
     3421 ggaag
//
