LOCUS       Exported                8350 bp ds-DNA     circular SYN 30-MAR-2021
DEFINITION  5 fragments assembled using overlaps.
ACCESSION   .
VERSION     .
KEYWORDS    pNW-pXynA-FnCpf1-Ppta-nt-No_flanks (auto2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 30, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    12..55
                     /label=term. loop 2
                     /note="term. loop of yhdG gene (B. subtilis)"
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     42..61
                     /label=spyCas9-seq-primer9-Fw
                     /note="from pNWspycas9"
                     /note="color: black; sequence: TTTCTGTTCTTGGCGACTCC"
     misc_feature    56..66
                     /label=AhdI
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    87..93
                     /label=BbvCI
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    106..111
                     /label=XhoI
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    115..162
                     /label=term. loop 3
                     /note="term. loop of yclE gene (B. subtilis)"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    163
                     /label=HindIII'
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     complement(356..374)
                     /label=BG3664
                     /note="pNW33n-Int-US-RV"
                     /note="color: black; sequence: AGGGCTCGCCTTTGGGAAG"
     primer_bind     complement(403..424)
                     /label=BG5536
                     /note="color: black; sequence: TATGAGATAATGCCGACTGTAC"
     primer_bind     complement(403..421)
                     /label=BG4759
                     /note="color: black; sequence: GAGATAATGCCGACTGTAC"
     misc_feature    complement(425..1075)
                     /label=Cat
                     /note="Derived from rom S. aureus plasmid pC194"
                     /note="color: #cc0000; direction: LEFT"
     primer_bind     846..869
                     /label=RepCat-Fw
                     /note="color: black; sequence: AACTCTCCGTCGCTATTGTAACCA"
     primer_bind     1076..1100
                     /label=BG5540 no res
                     /note="color: black; sequence: TTGATATGCCTCCTAAATTTTTATC"
     primer_bind     1113..1130
                     /label=BG4758
                     /note="color: black; sequence: GCCGATATCAGGAGGCTTACTTGTCTG"
     primer_bind     complement(1151..1172)
                     /label=BG4029
                     /note="om 3 pSH4-delta repB plasmiden te maken"
                     /note="color: black; sequence: GCCCGGGATTGACTTTTAAAAAAGG"
     primer_bind     complement(1151..1172)
                     /label=BG3993
                     /note="pSH4-delta-RepB-Fw"
                     /note="color: black; sequence: 
                     GCCGCTCTTCCGGGATTGACTTTTAAAAAAGG"
     primer_bind     complement(1151..1172)
                     /label=BG3894
                     /note="pEB1-2-delta-repB-Fw"
                     /note="color: black; sequence: 
                     GCCGATATCCGGGATTGACTTTTAAAAAAGG"
     primer_bind     1162..1181
                     /label=BG3716
                     /note="cut pNW-Fw"
                     /note="color: black; sequence: GCCAGTCAATCCCGTTTGTTGAA"
     primer_bind     1162..1181
                     /label=BG3717
                     /note="cut pNW-Fw+MCS"
                     /note="color: black; sequence: AGTCAATCCCGTTTGTTGAA"
     primer_bind     1170..1193
                     /label=BG5537
                     /note="color: black; sequence: 
                     GCCGATATCCGTTTGTTGAACTACTCTTTAAT"
     misc_feature    complement(1182..2186)
                     /label=repB
                     /note="Bacillus origin of replication from G. 
                     stearothermophilus plasmid pTHT15"
                     /note="color: #cc0000; direction: LEFT"
     primer_bind     1282..1301
                     /label=pNW33n repB Fw
                     /note="color: black; sequence: TCGCCTTCTTCTGTGTCATC"
     primer_bind     complement(1672..1692)
                     /label=RepCat-Rv
                     /note="color: black; sequence: TATGCGTGCAACGGAAGTGAC"
     primer_bind     complement(1883..1902)
                     /label=pNW33n repB Rv
                     /note="color: black; sequence: CTGGAGGAGAGCAATGAAAC"
     primer_bind     2149..2176
                     /label=BG4028
                     /note="om 3 pSH4-delta repB plasmiden te maken"
                     /note="color: black; sequence: 
                     GCCCTACTATTAGGACACATAATATTAAAAG"
     misc_feature    2332..2356
                     /label=oriB
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     2779..2799
                     /label=BG3994
                     /note="pSH4-delta-RepB-Rv"
                     /note="color: black; sequence: 
                     GCCGCTCTTCACGGTTATCCACAGAATCAGG"
     primer_bind     2779..2799
                     /label=BG3895
                     /note="pEB1-2-delta-RepB-Rv"
                     /note="color: black; sequence: 
                     GCCGATATCACGGTTATCCACAGAATCAGG"
     misc_feature    complement(2878..3466)
                     /label=ori
                     /note="E. coli origin of replication from plasmid pUC19"
                     /note="color: #cc0000; direction: LEFT"
     primer_bind     complement(3209..3228)
                     /label=BG3676
                     /note="color: black; sequence: GCTGCCAGTGGCGATAAGTC"
     primer_bind     3425..3445
                     /label=BG3859
                     /note="pNW33n-MCS-ori-Fw"
                     /note="color: black; sequence: GTTTGCAAGCAGCAGATTACG"
     misc_feature    3543..4036
                     /label=p_xynA'
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    3543..4036
                     /label=pXynA
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    4038..4042
                     /label='XbaI
                     /note="color: #cc0000; direction: RIGHT"
     CDS             4043..7945
                     /codon_start=1
                     /locus_tag="FTN_RS07150"
                     /old_locus_tag="FTN_1397"
                     /product="hypothetical protein"
                     /label=FnCas12a
                     /note="dnas_title=hypothetical protein"
                     /note="hypothetical protein"
                     /note="color: #cc0000"
                     /db_xref="GI:489130501"
                     /inference="EXISTENCE:same species:RefSeq"
                     /experiment="gene product confirmed by mass spectrometry"
                     /protein_id="WP_003040289.1"
                     /translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAK
                     DYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDT
                     IKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDID
                     EALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLK
                     DKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKF
                     NTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVID
                     KLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSL
                     TDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKL
                     ALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQA
                     SAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIV
                     PLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNK
                     KNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNH
                     STHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF
                     YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALF
                     DERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDK
                     RFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVD
                     GKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVV
                     HEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKT
                     GGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQE
                     FFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTR
                     EVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE
                     LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNL
                     VIKNEEYFEFVQNRNN"
     misc_feature    4230..4262
                     /label=PCR_primer
                     /note="SFnCpf001 For current=0"
                     /note="SFnCpf001 For current=0"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    complement(4230..4260)
                     /label=PCR_primer
                     /note="SFnCpf010"
                     /note="SFnCpf010"
                     /note="color: #cc0000; direction: LEFT"
     misc_feature    4885..4919
                     /label=SFnCpf002 For
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    7952..7994
                     /label=term. loop 1
                     /note="term. loop from yfjF gene (B.subtilis)"
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     7995..8021
                     /label=BG5317
                     /note="color: black; sequence: 
                     GCCGTCGACTTGCCGGAATTCTTTCACAGC"
     misc_feature    7995..8000
                     /label=SalI
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     8000..8018
                     /label=BG3887
                     /note="color: black; sequence: GCCGAGCTCTTGCCGGAATTCTTTCAC"
     misc_feature    8001..8289
                     /label=pPta
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     8001..8021
                     /label=BG5313
                     /note="color: black; sequence: 
                     GCCTCTAGATTGCCGGAATTCTTTCACAGC"
     primer_bind     8001..8020
                     /label=BG4441
                     /note="color: black; sequence: 
                     GCCTCTAGATTGCCGGAATTCTTTCACAG"
     primer_bind     8001..8018
                     /label=BG5402+res.site
                     /note="color: black; sequence: GCCTCTAGATTGCCGGAATTCTTTCAC"
     primer_bind     8001..8018
                     /label=BG3780
                     /note="Primer lacZ-pta-Fw, met overhang met primer 
                     pNW-4215-3570-Fw"
                     /note="color: black; sequence: TTGCCGGAATTCTTTCAC"
     primer_bind     complement(8006..8025)
                     /label=spyCas9-seq-primer2-Rv
                     /note="from pNWspycas9"
                     /note="color: black; sequence: TGCGGCTGTGAAAGAATTCC"
     misc_feature    8242..8247
                     /label=BspEI
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8290..8325
                     /label=Repeat
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8326..8350
                     /label=Spacer
                     /note="color: #cc0000; direction: RIGHT"
ORIGIN
        1 acgatagaca tataaagaga gcggccatac aggccgcctc ttttctgttc ttggcgactc
       61 cccgtctagt ggatcactca gcaactcctc agctgcagca gctgcctcga gtgcaaaaac
      121 agcccgcaga tcaacatccg cgggctgttt ctgattataa gaaagcttgg cgtaatcatg
      181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagccct tcaaacttcc
      361 caaaggcgag ccctagtgac attagaaaac cgactgtaaa aagtacagtc ggcattatct
      421 catattataa aagccagtca ttaggcctat ctgacaattc ctgaatagag ttcataaaca
      481 atcctgcatg ataaccatca caaacagaat gatgtacctg taaagatagc ggtaaatata
      541 ttgaattacc tttattaatg aattttcctg ctgtaataat gggtagaagg taattactat
      601 tattattgat atttaagtta aacccagtaa atgaagtcca tggaataata gaaagagaaa
      661 aagcattttc aggtataggt gttttgggaa acaatttccc cgaaccatta tatttctcta
      721 catcagaaag gtataaatca taaaactctt tgaagtcatt ctttacagga gtccaaatac
      781 cagagaatgt tttagataca ccatcaaaaa ttgtataaag tggctctaac ttatcccaat
      841 aacctaactc tccgtcgcta ttgtaaccag ttctaaaagc tgtatttgag tttatcaccc
      901 ttgtcactaa gaaaataaat gcagggtaaa atttatatcc ttcttgtttt atgtttcggt
      961 ataaaacact aatatcaatt tctgtggtta tactaaaagt cgtttgttgg ttcaaataat
     1021 gattaaatat ctcttttctc ttccaattgt ctaaatcaat tttattaaag ttcatttgat
     1081 atgcctccta aatttttatc taaagtgaat ttaggaggct tacttgtctg ctttcttcat
     1141 tagaatcaat ccttttttaa aagtcaatcc cgtttgttga actactcttt aataaaataa
     1201 tttttccgtt cccaattcca cattgcaata atagaaaatc catcttcatc ggctttttcg
     1261 tcatcatctg tatgaatcaa atcgccttct tctgtgtcat caaggtttaa ttttttatgt
     1321 atttctttta acaaaccacc ataggagatt aaccttttac ggtgtaaacc ttcctccaaa
     1381 tcagacaaac gtttcaaatt cttttcttca tcatcggtca taaaatccgt atcctttaca
     1441 ggatattttg cagtttcgtc aattgccgat tgtatatccg atttatattt atttttcggt
     1501 cgaatcattt gaacttttac atttggatca tagtctaatt tcattgcctt tttccaaaat
     1561 tgaatccatt gtttttgatt cacgtagttt tctgtattct taaaataagt tggttccaca
     1621 cataccaata catgcatgtg ctgattataa gaattatctt tattatttat tgtcacttcc
     1681 gttgcacgca taaaaccaac aagattttta ttaatttttt tatattgcat cattcggcga
     1741 aatccttgag ccatatctga caaactctta tttaattctt cgccatcata aacattttta
     1801 actgttaatg tgagaaacaa ccaacgaact gttggctttt gtttaataac ttcagcaaca
     1861 accttttgtg actgaatgcc atgtttcatt gctctcctcc agttgcacat tggacaaagc
     1921 ctggatttac aaaaccacac tcgatacaac tttctttcgc ctgtttcacg attttgttta
     1981 tactctaata tttcagcaca atcttttact ctttcagcct ttttaaattc aagaatatgc
     2041 agaagttcaa agtaatcaac attagcgatt ttcttttctc tccatggtct cacttttcca
     2101 ctttttgtct tgtccactaa aacccttgat ttttcatctg aataaatgct actattagga
     2161 cacataatat taaaagaaac ccccatctat ttagttattt gtttggtcac ttataacttt
     2221 aacagatggg gtttttctgt gcaaccaatt ttaagggttt tcaatacttt aaaacacata
     2281 cataccaaca cttcaacgca cctttcagca actaaaataa aaatgacgtt atttctatat
     2341 gtatcaagaa tagaaagaac tcgtttttcg ctacgctcaa aacgcaaaaa aagcactcat
     2401 tcgagtgctt tttcttatcg ctccaaatca tgcgattttt tcctctttgc ttttctttgc
     2461 tcacgaagtt ctcgatcacg ctgcaaaaca tcttgaagcg aaaaagtatt cttcttttct
     2521 tccgatcgct catgctgacg cacgaaaagc cctctaggcg cataggaaca actcctaaat
     2581 gcatgtgagg ggttttctcg tccatgtgaa cagtcgcata cgcaatattt tgtttcccat
     2641 actgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3301 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3541 gaatggcgct agtgagctga ggataatatt ttctatcata tttccactta gtgttccagt
     3601 attagcgaca ataacgcttt ttgttgcagt tggacaatgg aattcgtggt ttgatacaat
     3661 gcttttttgt tccatgaatc caaatttaag cacattacag tatgaattgc aaaaagtatt
     3721 gcagtcgact caacagttta cacaacaagc gtcttttgat atggggttaa gaaataattc
     3781 taattcagtt accccggaaa gcataagagc tgctatgacg gtagtggcag tggttcctat
     3841 tatgatggtt tatccattct tgcagaagta ttttgtaaag ggtttaacca ttggcagtgt
     3901 taagggttag tacatctttg gttttttata aatgctgaaa ggggggaaat ttaaaaatac
     3961 ttattagaca aaatttttta attgcgaggt gatagtaaat aaatttttca ataaattaaa
     4021 tttactgagg gaggaatcta gaatgtcaat ttatcaagaa tttgttaata aatatagttt
     4081 aagtaaaact ctaagatttg agttaatccc acagggtaaa acacttgaaa acataaaagc
     4141 aagaggtttg attttagatg atgagaaaag agctaaagac tacaaaaagg ctaaacaaat
     4201 aattgataaa tatcatcagt tttttataga ggagatatta agttcggttt gtattagcga
     4261 agatttatta caaaactatt ctgatgttta ttttaaactt aaaaagagtg atgatgataa
     4321 tctacaaaaa gattttaaaa gtgcaaaaga tacgataaag aaacaaatat ctgaatatat
     4381 aaaggactca gagaaattta agaatttgtt taatcaaaac cttatcgatg ctaaaaaagg
     4441 gcaagagtca gatttaattc tatggctaaa gcaatctaag gataatggta tagaactatt
     4501 taaagccaat agtgatatca cagatataga tgaggcgtta gaaataatca aatcttttaa
     4561 aggttggaca acttatttta agggttttca tgaaaataga aaaaatgttt atagtagcaa
     4621 tgatattcct acatctatta tttataggat agtagatgat aatttgccta aatttctaga
     4681 aaataaagct aagtatgaga gtttaaaaga caaagctcca gaagctataa actatgaaca
     4741 aattaaaaaa gatttggcag aagagctaac ctttgatatt gactacaaaa catctgaagt
     4801 taatcaaaga gttttttcac ttgatgaagt ttttgagata gcaaacttta ataattatct
     4861 aaatcaaagt ggtattacta aatttaatac tattattggt ggtaaatttg taaatggtga
     4921 aaatacaaag agaaaaggta taaatgaata tataaatcta tactcacagc aaataaatga
     4981 taaaacactc aaaaaatata aaatgagtgt tttatttaag caaattttaa gtgatacaga
     5041 atctaaatct tttgtaattg ataagttaga agatgatagt gatgtagtta caacgatgca
     5101 aagtttttat gagcaaatag cagcttttaa aacagtagaa gaaaaatcta ttaaagaaac
     5161 actatcttta ttatttgatg atttaaaagc tcaaaaactt gatttgagta aaatttattt
     5221 taaaaatgat aaatctctta ctgatctatc acaacaagtt tttgatgatt atagtgttat
     5281 tggtacagcg gtactagaat atataactca acaaatagca cctaaaaatc ttgataaccc
     5341 tagtaagaaa gagcaagaat taatagccaa aaaaactgaa aaagcaaaat acttatctct
     5401 agaaactata aagcttgcct tagaagaatt taataagcat agagatatag ataaacagtg
     5461 taggtttgaa gaaatacttg caaactttgc ggctattccg atgatatttg atgaaatagc
     5521 tcaaaacaaa gacaatttgg cacagatatc tatcaaatat caaaatcaag gtaaaaaaga
     5581 cctacttcaa gctagtgcgg aagatgatgt taaagctatc aaggatcttt tagatcaaac
     5641 taataatctc ttacataaac taaaaatatt tcatattagt cagtcagaag ataaggcaaa
     5701 tattttagac aaggatgagc atttttatct agtatttgag gagtgctact ttgagctagc
     5761 gaatatagtg cctctttata acaaaattag aaactatata actcaaaagc catatagtga
     5821 tgagaaattt aagctcaatt ttgagaactc gactttggct aatggttggg ataaaaataa
     5881 agagcctgac aatacggcaa ttttatttat caaagatgat aaatattatc tgggtgtgat
     5941 gaataagaaa aataacaaaa tatttgatga taaagctatc aaagaaaata aaggcgaggg
     6001 ttataaaaaa attgtttata aacttttacc tggcgcaaat aaaatgttac ctaaggtttt
     6061 cttttctgct aaatctataa aattttataa tcctagtgaa gatatactta gaataagaaa
     6121 tcattccaca catacaaaaa atggtagtcc tcaaaaagga tatgaaaaat ttgagtttaa
     6181 tattgaagat tgccgaaaat ttatagattt ttataaacag tctataagta agcatccgga
     6241 gtggaaagat tttggattta gattttctga tactcaaaga tataattcta tagatgaatt
     6301 ttatagagaa gttgaaaatc aaggctacaa actaactttt gaaaatatat cagagagcta
     6361 tattgatagc gtagttaatc agggtaaatt gtacctattc caaatctata ataaagattt
     6421 ttcagcttat agcaaagggc gaccaaatct acatacttta tattggaaag cgctgtttga
     6481 tgagagaaat cttcaagatg tggtttataa gctaaatggt gaggcagagc ttttttatcg
     6541 taaacaatca atacctaaaa aaatcactca cccagctaaa gaggcaatag ctaataaaaa
     6601 caaagataat cctaaaaaag agagtgtttt tgaatatgat ttaatcaaag ataaacgctt
     6661 tactgaagat aagtttttct ttcactgtcc tattacaatc aattttaaat ctagtggagc
     6721 taataagttt aatgatgaaa tcaatttatt gctaaaagaa aaagcaaatg atgttcatat
     6781 attaagtata gatagaggtg aaagacattt agcttactat actttggtag atggtaaagg
     6841 caatatcatc aaacaagata ctttcaacat cattggtaat gatagaatga aaacaaacta
     6901 ccatgataag cttgctgcaa tagagaaaga tagggattca gctaggaaag actggaaaaa
     6961 gataaataac atcaaagaga tgaaagaggg ctatctatct caggtagttc atgaaatagc
     7021 taagctagtt atagagtata atgctattgt ggtttttgag gatttaaatt ttggatttaa
     7081 aagagggcgt ttcaaggtag agaagcaggt ctatcaaaag ttagaaaaaa tgctaattga
     7141 gaaactaaac tatctagttt tcaaagataa tgagtttgat aaaactgggg gagtgcttag
     7201 agcttatcag ctaacagcac cttttgagac ttttaaaaag atgggtaaac aaacaggtat
     7261 tatctactat gtaccagctg gttttacttc aaaaatttgt cctgtaactg gttttgtaaa
     7321 tcagttatat cctaagtatg aaagtgtcag caaatctcaa gagttcttta gtaagtttga
     7381 caagatttgt tataaccttg ataagggcta ttttgagttt agttttgatt ataaaaactt
     7441 tggtgacaag gctgccaaag gcaagtggac tatagctagc tttgggagta gattgattaa
     7501 ctttagaaat tcagataaaa atcataattg ggatactcga gaagtttatc caactaaaga
     7561 gttggagaaa ttgctaaaag attattctat cgaatatggg catggcgaat gtatcaaagc
     7621 agctatttgc ggtgagagcg acaaaaagtt ttttgctaag ctaactagtg tcctaaatac
     7681 tatcttacaa atgcgtaact caaaaacagg tactgagtta gattatctaa tttcaccagt
     7741 agcagatgta aatggcaatt tctttgattc gcgacaggcg ccaaaaaata tgcctcaaga
     7801 tgctgatgcc aatggtgctt atcatattgg gctaaaaggt ctgatgctac taggtaggat
     7861 caaaaataat caagagggca aaaaactcaa tttggttatc aaaaatgaag agtattttga
     7921 gttcgtgcag aataggaata actaaatcag acaaaatggc ctgcttatga agcgggccat
     7981 ttttgtttaa tcctgtcgac ttgccggaat tctttcacag ccgcatccct ttcttccgca
     8041 ggcagcgcct tccagcccgc ccagtcgaga gaacggaaat catgcagtga gtaccagcca
     8101 tctagtgttt ctgctgcttc gctcattttt ctccctccaa atgtttttct cttcccttac
     8161 tataacacag tttagaagag ggagaaaata ttacggctgc cgcgaaattg tctaaaagaa
     8221 gccttctttt gcctttgatt ttccggaaaa atggattatt cttaaaatgg ataccgttat
     8281 acgaggagtg tctaagaact ttaaataatt tctactgttg tagatgagtg gatatggtca
     8341 acgtgcatgc
//
