LOCUS       Exported                9820 bp ds-DNA     circular SYN 30-MAR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 30, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      626..635
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             638..664
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     CDS             671..724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=BPSV40 NLS
                     /note="color: #993366"
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             725..4825
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /note="This feature has 3 segments:
                        1:  725 .. 3238 / #993366
                        2: 3239 .. 3241 / #cc99b2
                        3: 3242 .. 4825 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     1008..1034
                     /label=sp-seq1
                     /note="color: black; sequence: GCTTCTTCCACAGACTGGAAGAGTCCT;
                     added: 2021-01-18"
     primer_bind     1617..1638
                     /label=sp-seq2
                     /note="color: black; sequence: CCATCCTGCTGAGCGACATCCT; 
                     added: 2021-01-18"
     primer_bind     2224..2244
                     /label=sp-seq3
                     /note="color: black; sequence: CCTGCCCAACGAGAAGGTGCT; 
                     added: 2021-01-18"
     primer_bind     2811..2831
                     /label=sp-seq4
                     /note="color: black; sequence: TCCACGACGACAGCCTGACCT; 
                     added: 2021-01-18"
     primer_bind     3376..3397
                     /label=sp-seq5
                     /note="color: black; sequence: GCTGCTGAACGCCAAGCTGATT; 
                     added: 2021-01-18"
     primer_bind     3963..3981
                     /label=sp-seq6
                     /note="color: black; sequence: CCACCGTGCGGAAAGTGCT; added: 
                     2021-01-18"
     CDS             4052..4054
                     /codon_start=1
                     /label=L111
                     /note="color: #ff0000"
                     /translation="L"
     CDS             4124..4126
                     /codon_start=1
                     /label=D1135
                     /note="color: #ff0000"
                     /translation="D"
     CDS             4373..4375
                     /codon_start=1
                     /label=G1218
                     /note="color: #993366"
                     /translation="G"
     CDS             4376..4378
                     /codon_start=1
                     /label=E1219
                     /note="color: #993366"
                     /translation="E"
     primer_bind     4582..4601
                     /label=sp-seq7
                     /note="color: black; sequence: GGACAAAGTGCTGTCCGCCT; added:
                     2021-01-18"
     CDS             4685..4687
                     /codon_start=1
                     /label=1322A
                     /note="color: #993366"
                     /translation="A"
     CDS             4730..4732
                     /codon_start=1
                     /label=1337T
                     /note="color: #993366"
                     /translation="T"
     misc_feature    4925..6991
                     /label=M-MLV
                     /note="color: #008000"
     primer_bind     4987..5009
                     /label=MLV-seq1
                     /note="color: black; sequence: AGGGTCCACATGGCTGTCTGATT; 
                     added: 2021-01-18"
     primer_bind     5633..5654
                     /label=MLV-seq2
                     /note="color: black; sequence: CAACAAGGTACTCGGGCCCTGT; 
                     added: 2021-01-18"
     primer_bind     6288..6311
                     /label=MLV-seq3
                     /note="color: black; sequence: CCCGGATGACTCACTATCAGGCCT; 
                     added: 2021-01-18"
     misc_feature    6992..7045
                     /label=BP-SV40
                     /note="color: #00ffff"
     CDS             7055..7075
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7084..7101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     polyA_signal    7130..7354
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7425..7441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7449..7465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7473..7503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7473 .. 7479 / #ffffff / -10
                        2: 7480 .. 7497 / #ffffff
                        3: 7498 .. 7503 / #ffffff / -35"
     protein_bind    7518..7539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7827..8415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     7981..7990
                     /label=sp-seq7
                     /note="color: black; sequence: GGACAAAGTGCTGTCCGCCT; added:
                     2021-01-18"
     CDS             complement(8586..9446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8586 .. 9377 / #ccffcc
                        2: 9378 .. 9446 / #ccffcc / signal sequence
                     Cleavage site after base 9377"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9447..9551)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgcgccac catggggccc gccgccaagc gcgtgaagct
      661 ggacggcggc aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa
      721 agtcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
      781 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
      841 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
      901 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
      961 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     1021 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     1081 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     1141 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     1201 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     1261 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     1321 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     1381 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
     1441 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     1501 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     1561 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     1621 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     1681 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     1741 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     1801 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     1861 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     1921 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     1981 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     2041 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     2101 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     2161 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     2221 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     2281 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     2341 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     2401 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     2461 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     2521 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     2581 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     2641 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     2701 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     2761 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     2821 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     2881 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     2941 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     3001 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     3061 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     3121 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     3181 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
     3241 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     3301 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     3361 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
     3421 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     3481 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     3541 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
     3601 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     3661 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     3721 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     3781 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     3841 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     3901 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
     3961 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     4021 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
     4081 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     4141 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     4201 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     4261 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     4321 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     4381 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     4441 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     4501 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     4561 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
     4621 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     4681 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     4741 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     4801 cgacctgtct cagctgggag gtgactctgg aggatctagc ggaggatcct ctggcagcga
     4861 gacaccagga acaagcgagt cagcaacacc agagagcagt ggcggcagca gcggcggcag
     4921 cagcacccta aatatagaag atgagtatcg gctacatgag acctcaaaag agccagatgt
     4981 ttctctaggg tccacatggc tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat
     5041 gggactggca gttcgccaag ctcctctgat catacctctg aaagcaacct ctacccccgt
     5101 gtccataaaa caatacccca tgtcacaaga agccagactg gggatcaagc cccacataca
     5161 gagactgttg gaccagggaa tactggtacc ctgccagtcc ccctggaaca cgcccctgct
     5221 acccgttaag aaaccaggga ctaatgatta taggcctgtc caggatctga gagaagtcaa
     5281 caagcgggtg gaagacatcc accccaccgt gcccaaccct tacaacctct tgagcgggct
     5341 cccaccgtcc caccagtggt acactgtgct tgatttaaag gatgcctttt tctgcctgag
     5401 actccacccc accagtcagc ctctcttcgc ctttgagtgg agagatccag agatgggaat
     5461 ctcaggacaa ttgacctgga ccagactccc acagggtttc aaaaacagtc ccaccctgtt
     5521 taatgaggca ctgcacagag acctagcaga cttccggatc cagcacccag acttgatcct
     5581 gctacagtac gtggatgact tactgctggc cgccacttct gagctagact gccaacaagg
     5641 tactcgggcc ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc
     5701 ccaaatttgc cagaaacagg tcaagtatct ggggtatctt ctaaaagagg gtcagagatg
     5761 gctgactgag gccagaaaag agactgtgat ggggcagcct actccgaaga cccctcgaca
     5821 actaagggag ttcctaggga aggcaggctt ctgtcgcctc ttcatccctg ggtttgcaga
     5881 aatggcagcc cccctgtacc ctctcaccaa accggggact ctgtttaatt ggggcccaga
     5941 ccaacaaaag gcctatcaag aaatcaagca agctcttcta actgccccag ccctggggtt
     6001 gccagatttg actaagccct ttgaactctt tgtcgacgag aagcagggct acgccaaagg
     6061 tgtcctaacg caaaaactgg gaccttggcg tcggccggtg gcctacctgt ccaaaaagct
     6121 agacccagta gcagctgggt ggcccccttg cctacggatg gtagcagcca ttgccgtact
     6181 gacaaaggat gcaggcaagc taaccatggg acagccacta gtcattctgg ccccccatgc
     6241 agtagaggca ctagtcaaac aaccccccga ccgctggctt tccaacgccc ggatgactca
     6301 ctatcaggcc ttgcttttgg acacggaccg ggtccagttc ggaccggtgg tagccctgaa
     6361 cccggctacg ctgctcccac tgcctgagga agggctgcaa cacaactgcc ttgatatcct
     6421 ggccgaagcc cacggaaccc gacccgacct aacggaccag ccgctcccag acgccgacca
     6481 cacctggtac acggatggaa gcagtctctt acaagaggga cagcgtaagg cgggagctgc
     6541 ggtgaccacc gagaccgagg taatctgggc taaagccctg ccagccggga catccgctca
     6601 gcgggctgaa ctgatagcac tcacccaggc cctaaagatg gcagaaggta agaagctaaa
     6661 tgtttatact gatagccgtt atgcttttgc tactgcccat atccatggag aaatatacag
     6721 aaggcgtggg tggctcacat cagaaggcaa agagatcaaa aataaagacg agatcttggc
     6781 cctactaaaa gccctctttc tgcccaaaag acttagcata atccattgtc caggacatca
     6841 aaagggacac agcgccgagg ctagaggcaa ccggatggct gaccaagcgg cccgaaaggc
     6901 agccatcaca gagactccag acacctctac cctcctcata gaaaattcat caccctctgg
     6961 cggctcaaaa agaaccgccg acggcagcga gaagcgcacc gccgacagcc agcacagcac
     7021 cccccccaag accaagcgca aggtggaatt cgagcccaag aagaagagga aagtctaacc
     7081 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     7141 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7201 actcccactg tcctttccta ataaaatgag aaaattgcat cgcattgtct gagtaggtgt
     7261 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7321 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7381 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7441 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7501 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7561 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7621 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7681 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7741 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8461 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8521 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8581 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8641 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8701 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8761 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8821 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8881 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8941 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9001 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9061 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9121 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9181 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9241 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9301 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9361 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9421 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9481 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9541 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
     9601 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
     9661 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9721 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9781 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
