LOCUS       Exported                9870 bp ds-DNA     circular SYN 04-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Apr 4, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             37..57
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             58..4158
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4258..6300
                     /label=M-MLV
     CDS             6328..6417
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    6418..6468
                     /label=BPSV40
     CDS             6477..6494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6523..6747
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6818..6834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6842..6858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6866..6896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6911..6932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7220..7808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7979..8839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8840..8944)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9214..9593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9594..9797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        9839..9857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac
       61 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
      121 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
      181 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
      241 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
      301 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
      361 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
      421 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
      481 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
      541 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
      601 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
      661 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
      721 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
      781 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
      841 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
      901 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
      961 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
     1021 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
     1081 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
     1141 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
     1201 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
     1261 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
     1321 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
     1381 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
     1441 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
     1501 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
     1561 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
     1621 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
     1681 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
     1741 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
     1801 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
     1861 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
     1921 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
     1981 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
     2041 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
     2101 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
     2161 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
     2221 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
     2281 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
     2341 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
     2401 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
     2461 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
     2521 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg
     2581 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
     2641 aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
     2701 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
     2761 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
     2821 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
     2881 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
     2941 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
     3001 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
     3061 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
     3121 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
     3181 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
     3241 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
     3301 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
     3361 ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga
     3421 aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct
     3481 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
     3541 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
     3601 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
     3661 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag
     3721 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
     3781 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
     3841 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
     3901 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
     3961 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc
     4021 gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg
     4081 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
     4141 tctcagctgg gaggtgactc tggaggatct agcggaggat cctctggcag cgagacacca
     4201 ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg cagcagcacc
     4261 ctaaatatag aagatgagta tcggctacat gagacctcaa aagagccaga tgtttctcta
     4321 gggtccacat ggctgtctga ttttcctcag gcctgggcgg aaaccggggg catgggactg
     4381 gcagttcgcc aagctcctct gatcatacct ctgaaagcaa cctctacccc cgtgtccata
     4441 aaacaatacc ccatgtcaca agaagccaga ctggggatca agccccacat acagagactg
     4501 ttggaccagg gaatactggt accctgccag tccccctgga acacgcccct gctacccgtt
     4561 aagaaaccag ggactaatga ttataggcct gtccaggatc tgagagaagt caacaagcgg
     4621 gtggaagaca tccaccccac cgtgcccaac ccttacaacc tcttgagcgg gctcccaccg
     4681 tcccaccagt ggtacactgt gcttgattta aaggatgcct ttttctgcct gagactccac
     4741 cccaccagtc agcctctctt cgcctttgag tggagagatc cagagatggg aatctcagga
     4801 caattgacct ggaccagact cccacagggt ttcaaaaaca gtcccaccct gtttaatgag
     4861 gcactgcaca gagacctagc agacttccgg atccagcacc cagacttgat cctgctacag
     4921 tacgtggatg acttactgct ggccgccact tctgagctag actgccaaca aggtactcgg
     4981 gccctgttac aaaccctagg gaacctcggg tatcgggcct cggccaagaa agcccaaatt
     5041 tgccagaaac aggtcaagta tctggggtat cttctaaaag agggtcagag atggctgact
     5101 gaggccagaa aagagactgt gatggggcag cctactccga agacccctcg acaactaagg
     5161 gagttcctag ggaaggcagg cttctgtcgc ctcttcatcc ctgggtttgc agaaatggca
     5221 gcccccctgt accctctcac caaaccgggg actctgttta attggggccc agaccaacaa
     5281 aaggcctatc aagaaatcaa gcaagctctt ctaactgccc cagccctggg gttgccagat
     5341 ttgactaagc cctttgaact ctttgtcgac gagaagcagg gctacgccaa aggtgtccta
     5401 acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc tgtccaaaaa gctagaccca
     5461 gtagcagctg ggtggccccc ttgcctacgg atggtagcag ccattgccgt actgacaaag
     5521 gatgcaggca agctaaccat gggacagcca ctagtcattc tggcccccca tgcagtagag
     5581 gcactagtca aacaaccccc cgaccgctgg ctttccaacg cccggatgac tcactatcag
     5641 gccttgcttt tggacacgga ccgggtccag ttcggaccgg tggtagccct gaacccggct
     5701 acgctgctcc cactgcctga ggaagggctg caacacaact gccttgatat cctggccgaa
     5761 gcccacggaa cccgacccga cctaacggac cagccgctcc cagacgccga ccacacctgg
     5821 tacacggatg gaagcagtct cttacaagag ggacagcgta aggcgggagc tgcggtgacc
     5881 accgagaccg aggtaatctg ggctaaagcc ctgccagccg ggacatccgc tcagcgggct
     5941 gaactgatag cactcaccca ggccctaaag atggcagaag gtaagaagct aaatgtttat
     6001 actgatagcc gttatgcttt tgctactgcc catatccatg gagaaatata cagaaggcgt
     6061 gggtggctca catcagaagg caaagagatc aaaaataaag acgagatctt ggccctacta
     6121 aaagccctct ttctgcccaa aagacttagc ataatccatt gtccaggaca tcaaaaggga
     6181 cacagcgccg aggctagagg caaccggatg gctgaccaag cggcccgaaa ggcagccatc
     6241 acagagactc cagacacctc taccctcctc atagaaaatt catcaccctc tggcggctca
     6301 aaaagaaccg ccgacggcag cgaattctac ccatacgatg ttcctgacta tgcgggctat
     6361 ccctatgacg tcccggacta tgcaggatcg tatccttatg acgttccaga ttacgctaaa
     6421 agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcta accggtcatc
     6481 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6541 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6601 ctgtcctttc ctaataaaat gagaaaattg catcgcattg tctgagtagg tgtcattcta
     6661 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6721 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     6781 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6901 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6961 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7021 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7081 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7141 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7621 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7861 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8941 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg
     9001 atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9061 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     9121 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     9181 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     9241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     9301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     9361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     9421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     9481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     9541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     9601 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     9661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     9721 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     9781 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta
     9841 atacgactca ctatagggag agccgccacc
//
