LOCUS       pEPIC1.1                4663 bp    DNA     circular UNA 01-APR-2021
DEFINITION  .
ACCESSION   urn.local...2o-d72pwud
VERSION     urn.local...2o-d72pwud
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..313
                     /standard_name="ePiggyBac Left 5' ITR"
     regulatory      324..823
                     /note="Geneious type: promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /standard_name="PGK promoter"
     regulatory      827..835
                     /standard_name="Kozak sequence"
     misc_feature    835..854
                     /note="Geneious type: polylinker"
                     /standard_name="MCS"
     CDS             866..931
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /standard_name="3xFLAG"
     CDS             947..970
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an
                     engineered form of streptavidin"
                     /translation="WSHPQFEK"
                     /standard_name="Strep-Tag II"
     CDS             1007..1033
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an
                     engineered form of streptavidin"
                     /translation="WSHPQFEK"
                     /standard_name="Strep-Tag II"
     misc_feature    1069..1630
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /standard_name="IRES"
     CDS             1642..2361
                     /standard_name="mClover3"
     regulatory      2370..2860
                     /note="Geneious type: terminator"
                     /standard_name="Rabbit B-globlin polyA"
     misc_feature    complement(2880..3114)
                     /standard_name="PiggyBac Right 3' ITR"
     regulatory      3119..3144
                     /note="Geneious type: promoter"
                     /standard_name="amp prom"
     CDS             3186..4045
                     /standard_name="Bla (AmpR)"
     rep_origin      4061..4663
ORIGIN      
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacaacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttcg tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcagggaca gccgggtagg ggaggcgctt ttcccaaggc agtctggagc
      361 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
      421 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
      481 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
      541 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      601 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      661 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      721 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      781 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgattgccg ccaccttaag
      841 gtggacatct cgaggttcag caggtgatta caaggaccat gatggcgatt ataaggatca
      901 cgacatcgac tacaaagatg acgatgacaa gggtgccagc agcgcatggt cccaccctca
      961 gtttgagaag ggcggaggct ctggcggcgg aagcggagga tctgcttgga gccaccccca
     1021 gtttgaaaag taatgagaat tcgactggtt cacactatat ctgctctacc cccccctaac
     1081 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     1141 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     1201 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     1261 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     1321 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     1381 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     1441 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     1501 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     1561 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     1621 gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1681 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt ccgcggcgag
     1741 ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
     1801 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcgtggc ctgcttcagc
     1861 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1921 gtccaggagc gcaccatctc tttcaaggac gacggtacct acaagacccg cgccgaggtg
     1981 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2041 gacggcaaca tcctggggca caagctggag tacaacttca acagccacta cgtctatatc
     2101 acggccgaca agcagaagaa ctgcatcaag gctaacttca agatccgcca caacgttgag
     2161 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2221 gtgctgctgc ccgacaacca ctacctgagc catcagtcca agctgagcaa agaccccaac
     2281 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccggaat aacgcacggc
     2341 atggacgagc tgtacaagtg aagcggccgg ccgccagcac agtggtcgat cgaccaatgc
     2401 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2461 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2521 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     2581 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     2641 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     2701 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     2761 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     2821 cctctcctga ctactcccag tcatagctgt ccctcttctc ggctgtccct cataaaagtt
     2881 ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata
     2941 aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata
     3001 tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt
     3061 tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg ttaacgagaa
     3121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4021 gtgcctcact gattaagcat tggtacccgt agaaaagatc aaaggatctt cttgagatcc
     4081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aac
//
