LOCUS       pPID2                   2427 bp    DNA     circular UNA 01-APR-2021
DEFINITION  .
ACCESSION   urn.local...1x-d72pq9v
VERSION     urn.local...1x-d72pq9v
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..313
                     /standard_name="ePiggyBac Left 5' ITR"
     misc_feature    314..378
                     /note="Geneious type: motif"
                     /standard_name="65bp insulator sequence"
     misc_feature    379..522
                     /note="Geneious type: polylinker"
                     /standard_name="MCS"
     misc_feature    complement(523..587)
                     /note="Geneious type: motif"
                     /standard_name="65bp insulator sequence"
     misc_feature    complement(588..822)
                     /standard_name="PiggyBac Right 3' ITR"
     regulatory      823..851
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     CDS             893..1753
                     /standard_name="Bla (AmpR)"
     repeat_region   1753..1824
                     /note="72 bp tandem repeat enhancer sequence A [83],[78],
                     [81],[110]"
                     /standard_name="SV40 72bp tandem repeat DTS"
     rep_origin      1825..2427
ORIGIN      
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacaacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttcg tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcagagggg cggaagggac gttaggaggg aggcagggag gcagggaggc
      361 agggaggaac ggagggagcg acagtggtaa gcttctagat cgattataac gcgtgtcgac
      421 tcgagaattc tgcaggtacc atggccggct taagacgtca attgctagca cgtgttaaca
      481 ctagtacgta catgttggcc agatctctag atgactcgtg ctctccctcc gttcctccct
      541 gcctccctgc ctccctgcct ccctcctaac gtcccttccg cccctctttt gttactttat
      601 agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt
      661 gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt
      721 aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta
      781 tctttaacgt acgtcacaat atgattatct ttctagggtt aattcaaata tgtatccgct
      841 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      901 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      961 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1021 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1081 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1141 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1201 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1261 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1321 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1381 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1441 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1501 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1561 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1621 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1681 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1741 taagcattgg taggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1801 tgcatctcaa ttagtcagca accaaaagga tcttcttgag atcctttttt tctgcgcgta
     1861 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1921 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1981 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2041 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2101 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2161 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2221 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2281 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2341 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2401 tcaggggggc ggagcctatg gaaaaac
//
