LOCUS       AT6\CMV-VPR-S-L1-dCjCas9-HNH\GSK\linker-D2\vp64        6839 bp    DNA     circular SYN 16-JAN-2020
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2017Äê5ÔÂ25ÈÕ from SnapGene Viewer 2.8.3 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13169549|
COMMENT     VNTDBDATE|-13169549|
COMMENT     LSOWNER|
COMMENT     VNTNAME|AT6 CMV-VPR-S-L1-dCjCas9-HNH GSK linker-D2 vp64|
COMMENT     VNTAUTHORNAME|ZXX|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT         "BsmAI" "BsmBI" "BsrGI" "DpnI" "DraI" "EaeI" "EagI" "EcoRV" "HpaI" 
COMMENT         "KpnI" "MfeI" "MluI" "NdeI" "NheI" "NotI" "PacI" "PmeI" "PmlI" "PvuI" 
COMMENT         "PvuII" "RsrII" "SacI" "SacII" "SalI" "ScaI" "SfiI" "SnaBI" "SpeI" 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 5 41 0 5645 0 6196 0 6298 0 
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COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 3454 0 4153 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4741 0 5368 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3239 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 2960 0 3251 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 3626 0 4942 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 5647 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 5645 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 63 0 4527 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 301 0 3896 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "BclI" "tgatca" 2 0 4 99 0 4496 0 5285 0 5999 
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COMMENT        #15=(CRSite (CStringList) "BglII" "agatct" 2 0 4 2362 0 3690 0 6092 0 
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COMMENT        #16=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1488 1 3226 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 8 1488 1 2264 0 3034 0 
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COMMENT        #18=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 3 4700 0 5057 0 6527 0 0 
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COMMENT        #23=(CRSite (CStringList) "EagI" "cggccg" 2 0 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 2511 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 2578 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 2834 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 1 5898 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 1797 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 358 0 3726 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "RsrII" "cggwccg" 3 0 1 4162 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "SacI" "gagctc" 6 0 3 95 0 3168 0 3654 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2348 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 1907 0 4711 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 1 2940 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2599 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "SphI" "gcatgc" 6 0 4 50 0 312 0 4105 0 5919 0 
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COMMENT        #46=(CRSite (CStringList) "StuI" "aggcct" 4 0 3 4480 0 5452 0 6034 0 0 
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COMMENT        #48=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 41 0 6298 0 0 "") 
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COMMENT        #54=(CRSite (CStringList) "tca" "tca" 1 0 117 76 0 101 0 241 0 428 0 472 
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COMMENT        #55=(CRSite (CStringList) "tag" "tag" 1 0 47 54 0 71 0 88 0 121 0 235 0 
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COMMENT        #56=(CRSite (CStringList) "cta" "cta" 1 0 71 12 0 27 0 33 0 53 0 63 0 68 
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COMMENT       (CObList 
COMMENT        #57=(CFSignal (CObList) "HA" 4 0 0 7 33 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1" 
COMMENT              "/product=\"HA (human influenza hemagglutinin) epitope tag\"") 1 1 
COMMENT             1 1 "7..33") 
COMMENT        #58=(CFSignal (CObList) "SP6 promoter" 30 0 1 67 85 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(67..85)") 
COMMENT        #59=(CFSignal (CObList) "bGH poly(A) signal" 25 0 0 111 335 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "111..335") 
COMMENT        #60=(CFSignal (CObList) "ori" 33 0 1 594 1182 0 (CStringList) 
COMMENT             (CStringList "/direction=LEFT") 1 1 1 1 "complement(594..1182)") 
COMMENT        #61=(CFSignal (CObList) "AmpR" 4 0 1 1353 2213 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1" "/gene=\"bla\"" 
COMMENT              "/product=\"beta-lactamase\"") 1 1 1 1 "complement(1353..2213)") 
COMMENT        #62=(CFSignal (CObList) "AmpR promoter" 30 0 1 2214 2318 0 (CStringList) 
COMMENT             (CStringList "/gene=\"bla\"") 1 1 1 1 "complement(2214..2318)") 
COMMENT        #63=(CFSignal (CObList) "SV40 NLS" 4 0 0 6825 6 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1" 
COMMENT              "/product=\"nuclear localization signal of SV40 large T" 
COMMENT              "antigen\"") 1 1 1 1 "6822..6") 
COMMENT        #64=(CFSignal (CObList) "poly A-R" 27 0 0 91 110 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "91..110") 
COMMENT        #65=(CFSignal (CObList) "CJ cas9" 4 0 0 4203 33 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4200..33") 
COMMENT        #66=(CFSignal (CObList) "D8A" 61 0 0 4224 4226 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4221..4223") 
COMMENT        #67=(CFSignal (CObList) "!!!!" 21 0 0 6416 6416 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6413..6413") 
COMMENT        #68=(CFSignal (CObList) "CjCas9-seq-F1" 21 0 0 4673 4692 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4670..4689") 
COMMENT        #69=(CFSignal (CObList) "CjCas9-seq-F2" 21 0 0 5568 5587 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5565..5584") 
COMMENT        #70=(CFSignal (CObList) "CjCas9-seq-F3" 21 0 0 6124 6143 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6121..6140") 
COMMENT        #71=(CFSignal (CObList) "L1-seq-R" 21 0 0 4287 4306 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4284..4303") 
COMMENT        #72=(CFSignal (CObList) "R1" 21 0 0 4132 4171 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4129..4168") 
COMMENT        #73=(CFSignal (CObList) "F2" 21 0 0 4132 4170 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4129..4167") 
COMMENT        #74=(CFSignal (CObList) "R2" 21 0 0 4177 4216 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4174..4213") 
COMMENT        #75=(CFSignal (CObList) "F1" 21 0 0 2579 2623 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2579..2623") 
COMMENT        #76=(CFSignal (CObList) "R2" 21 0 0 3219 3263 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3219..3263") 
COMMENT        #77=(CFSignal (CObList) "GSK linker" 21 0 0 5682 5690 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5679..5687") 
COMMENT        #78=(CFSignal (CObList) "L1-D2" 21 0 0 4152 4196 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4149..4193") 
COMMENT        #79=(CFSignal (CObList) "CMV promoter" 21 0 0 2584 3167 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2584..3167") 
COMMENT        #80=(CFSignal (CObList) "VPR-S new" 21 0 0 3252 4151 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList #81=(CORF "atg" "taa" 3 0 3123 33 0 0 0 0 30) 
COMMENT        #82=(CORF "atg" "tga" 1 1 4041 5975 1702060386 0 0 0 30) 
COMMENT        #83=(CORF "atg" "tga" 3 1 4041 48 1633905005 0 0 0 30)) 
COMMENT       (CTextView 0 
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COMMENT                    "AT6 CMV-VPR-S-L1-dCjCas9-HNH GSK linker-D2 vp64" 2) 5 "" 0 
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COMMENT                          "DNA 'AT6 CMV-VPR-S-L1-dCjCas9-HNH GSK linker-D2 vp64'" 
COMMENT                          1) 
COMMENT                     #88=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #89=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/16/20 10:44\311\317\316\347" 1) 
COMMENT                     #90=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/16/20 10:44\311\317\316\347" 1) 
COMMENT                     #91=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6839 bp" 1) 
COMMENT                     #92=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #93=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #96=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: SYN" 1) 
COMMENT                     #97=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 11-APR-2019" 1) 
COMMENT                     #98=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: synthetic DNA construct." 1) 
COMMENT                     #99=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
COMMENT                          1) 
COMMENT                     #100=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                           "Associated Genes:  bla" 1))) "Standard Fields") 
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COMMENT                            "REFERENCE   1  (bases 1 to 6231)" 1) 
COMMENT                      #103=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "  AUTHORS   ." 1) 
COMMENT                      #104=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "  TITLE     Direct Submission" 1) 
COMMENT                      #105=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "  JOURNAL   Exported 2017\304\3525\324\30225\310\325 from SnapGene Viewer 2.8.3 to Vector NTI(R) " 
COMMENT                            1) 
COMMENT                      #106=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "            format" 1) 
COMMENT                      #107=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "            http://www.snapgene.com" 1))) 
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COMMENT                             (CGroupPar (CParagraph 63084872 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BsmAI: 8 sites") 6 10 
COMMENT                            "GTCTCNNNNNN" "CAGAGNNNNNN") 
COMMENT                      #255=(CRSFolderPar 
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COMMENT                            "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
COMMENT                      #256=(CRSFolderPar 
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COMMENT                              (CObjectList)) "BsrGI: 3 sites") 1 5 "TGTACA" 
COMMENT                            "ACATGT") 
COMMENT                      #257=(CRSFolderPar 
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COMMENT                            "TAGCTA") 
COMMENT                      #258=(CRSFolderPar 
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COMMENT                              (CObjectList)) "DpnI: 49 sites") 2 2 "GATC" "CTAG") 
COMMENT                      #259=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63084488 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "DraI: 3 sites") 3 3 "TTTAAA" 
COMMENT                            "AAATTT") 
COMMENT                      #260=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085224 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EaeI: 9 sites") 1 5 "YGGCCR" 
COMMENT                            "RCCGGY") 
COMMENT                      #261=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085256 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EagI: 0 sites") 1 5 "CGGCCG" 
COMMENT                            "GCCGGC") 
COMMENT                      #262=(CRSFolderPar 
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COMMENT                                            " N1: 4741 " 1) 
COMMENT                                      #265=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5368 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #266=(CRSFolderPar 
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COMMENT                              (CObjectList)) "EcoRV: 0 sites") 3 3 "GATATC" 
COMMENT                            "CTATAG") 
COMMENT                      #267=(CRSFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #268=(CGroupPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3239 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #270=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63084584 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HpaI: 0 sites") 3 3 "GTTAAC" 
COMMENT                            "CAATTG") 
COMMENT                      #271=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63084648 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" 
COMMENT                            "CCATGG") 
COMMENT                      #272=(CRSFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #274=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2511 " 1))))) "MfeI: 1 site") 
COMMENT                            1 5 "CAATTG" "GTTAAC") 
COMMENT                      #275=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63084680 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #276=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #28# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #277=(CLinePar 
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COMMENT                                            " N1: 2578 " 1))))) "MluI: 1 site") 
COMMENT                            1 5 "ACGCGT" "TGCGCA") 
COMMENT                      #278=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63111208 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #279=(CGroupPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 2960 " 1) 
COMMENT                                      #281=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3251 " 1))))) "NcoI: 2 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #282=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63084712 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #283=(CGroupPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 2834 " 1))))) "NdeI: 1 site") 
COMMENT                            2 4 "CATATG" "GTATAC") 
COMMENT                      #285=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63084904 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #286=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085672 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
COMMENT                      #287=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086024 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                            "AATTAATT") 
COMMENT                      #288=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085512 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PmeI: 0 sites") 4 4 "GTTTAAAC" 
COMMENT                            "CAAATTTG") 
COMMENT                      #289=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085704 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 5898 " 1))))) "PmlI: 1 site") 
COMMENT                            3 3 "CACGTG" "GTGCAC") 
COMMENT                      #292=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63111784 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #293=(CGroupPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3626 " 1) 
COMMENT                                      #295=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4942 " 1))))) "PstI: 2 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #296=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #297=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #35# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #298=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1797 " 1))))) "PvuI: 1 site") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #299=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086248 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #300=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #36# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #301=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 358 " 1) 
COMMENT                                      #302=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3726 " 1))))) "PvuII: 2 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #303=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085928 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #304=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #37# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #305=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4162 " 1))))) "RsrII: 1 site") 
COMMENT                            2 5 "CGGWCCG" "GCCWGGC") 
COMMENT                      #306=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085544 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SacI: 3 sites") 5 1 "GAGCTC" 
COMMENT                            "CTCGAG") 
COMMENT                      #307=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085960 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SacII: 0 sites") 4 2 "CCGCGG" 
COMMENT                            "GGCGCC") 
COMMENT                      #308=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086152 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #309=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #40# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #310=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2348 " 1))))) "SalI: 1 site") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #311=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085992 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #312=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #41# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #313=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1907 " 1) 
COMMENT                                      #314=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4711 " 1))))) "ScaI: 2 sites") 
COMMENT                            3 3 "AGTACT" "TCATGA") 
COMMENT                      #315=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086120 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SfiI: 0 sites") 8 5 
COMMENT                            "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
COMMENT                      #316=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63111240 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #317=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #318=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5647 " 1))))) "SmaI: 1 site") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #319=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085800 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #320=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #43# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #321=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #43# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2940 " 1))))) "SnaBI: 1 site") 
COMMENT                            3 3 "TACGTA" "ATGCAT") 
COMMENT                      #322=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63085864 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #323=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #44# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #324=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #44# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2599 " 1))))) "SpeI: 1 site") 
COMMENT                            1 5 "ACTAGT" "TGATCA") 
COMMENT                      #325=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086440 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SphI: 4 sites") 5 1 "GCATGC" 
COMMENT                            "CGTACG") 
COMMENT                      #326=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086344 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "StuI: 3 sites") 3 3 "AGGCCT" 
COMMENT                            "TCCGGA") 
COMMENT                      #327=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086184 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #328=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #47# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #329=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #47# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 53 " 1))))) "XbaI: 1 site") 1 
COMMENT                            5 "TCTAGA" "AGATCT") 
COMMENT                      #330=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63086216 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #331=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #48# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #332=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #48# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 41 " 1) 
COMMENT                                      #333=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #48# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6298 " 1))))) "XhoI: 2 sites") 
COMMENT                            1 5 "CTCGAG" "GAGCTC") 
COMMENT                      #334=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63111688 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #335=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #336=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5645 " 1))))) "XmaI: 1 site") 
COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map:  (no more than 2 sites)") 
COMMENT              #337=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #338=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #339=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 1 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame 1 (0 ORFs)") 
COMMENT                              #340=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 2 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame 2 (0 ORFs)") 
COMMENT                              #341=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #342=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 166823280 (4 #81# 0) 1 
COMMENT                                              2 3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #343=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #344=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3123 - 33" 1))) 
COMMENT                                            "3123(d3) (1250 codons)"))) 
COMMENT                                    "Reading Frame 3 (1 ORF)"))) 
COMMENT                            "Direct(Forward) strand") 
COMMENT                      #345=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #346=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 -1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #347=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 166823448 (4 #82# 0) 1 
COMMENT                                              2 3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #348=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #349=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4041 - 5975" 
COMMENT                                                    1))) "4041(c1) (645 codons)"))) 
COMMENT                                    "Reading Frame 1 (1 ORF)") 
COMMENT                              #350=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 2 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame 2 (0 ORFs)") 
COMMENT                              #351=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 -3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #352=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 166823952 (4 #83# 0) 1 
COMMENT                                              2 3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #353=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #354=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4041 - 48" 1))) 
COMMENT                                            "4041(c3) (949 codons)"))) 
COMMENT                                    "Reading Frame 3 (1 ORF)"))) 
COMMENT                            "Complementary(Reverse) strand"))) 
COMMENT                    "Open Reading Frames")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #356=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #357=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #358=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #359=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #360=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #361=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #362=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #363=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #364=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #365=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #366=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #367=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #368=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #369=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #370=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #371=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #372=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #373=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #374=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #375=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #376=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #377=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
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FEATURES             Location/Qualifiers
     CDS             7..33
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /vntifkey="4"
                     /label=HA
     promoter        complement(67..85)
                     /vntifkey="30"
                     /label=SP6\promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    111..335
                     /vntifkey="25"
                     /label=bGH\poly(A)\signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(594..1182)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1353..2213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2214..2318)
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     CDS             6825..6
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /vntifkey="4"
                     /label=SV40\NLS
     primer          91..110
                     /vntifkey="27"
                     /label=poly\A-R
     CDS             4203..33
                     /vntifkey="4"
                     /label=CJ\cas9
     exon            4224..4226
                     /vntifkey="61"
                     /label=D8A
     misc_feature    6416..6416
                     /vntifkey="21"
                     /label=!!!!
     misc_feature    4673..4692
                     /vntifkey="21"
                     /label=CjCas9-seq-F1
     misc_feature    5568..5587
                     /vntifkey="21"
                     /label=CjCas9-seq-F2
     misc_feature    6124..6143
                     /vntifkey="21"
                     /label=CjCas9-seq-F3
     misc_feature    4287..4306
                     /vntifkey="21"
                     /label=L1-seq-R
     misc_feature    4132..4171
                     /vntifkey="21"
                     /label=R1
     misc_feature    4132..4170
                     /vntifkey="21"
                     /label=F2
     misc_feature    4177..4216
                     /vntifkey="21"
                     /label=R2
     misc_feature    2579..2623
                     /vntifkey="21"
                     /label=F1
     misc_feature    3219..3263
                     /vntifkey="21"
                     /label=R2
     misc_feature    5682..5690
                     /vntifkey="21"
                     /label=GSK\linker
     misc_feature    4152..4196
                     /vntifkey="21"
                     /label=L1-D2
     misc_feature    2584..3167
                     /vntifkey="21"
                     /label=CMV\promoter
     misc_feature    3252..4151
                     /vntifkey="21"
                     /label=VPR-S\new
BASE COUNT     1790 a      1826 c      1804 g      1419 t 
ORIGIN
        1 aaagtatacc cctacgacgt gcccgactac gcctaataac tcgagcatgc atctagaggg 
       61 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc 
      121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
      181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
      241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
      301 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
      361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
      421 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
      481 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
      541 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
      601 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
      661 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
      721 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
      781 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
      841 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
      901 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
      961 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     1021 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     1081 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     1141 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     1201 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     1261 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     1321 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     1381 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     1441 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     1501 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     1561 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     1621 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     1681 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     1741 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     1801 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     1861 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     1921 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     1981 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     2041 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     2101 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     2161 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     2221 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     2281 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     2341 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct 
     2401 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 
     2461 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 
     2521 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc 
     2581 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
     2641 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
     2701 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     2761 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     2821 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     2881 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
     2941 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 
     3001 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 
     3061 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 
     3121 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta 
     3181 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag 
     3241 cttgggccac catggacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc 
     3301 tcgatgattt tgaccttgac atgcttggta gtgatgccct tgatgacttt gacctcgaca 
     3361 tgctcggcag tgacgccctt gatgatttcg acctggacat gctgggagga agcggaggag 
     3421 gaggtagcgg acctaagaaa aagaggaagg tgggatccgt gctggcccag actatggtgc 
     3481 cctctagtgc tatggtgcct ctggcccagc cacctgctcc agcccctgtg ctgaccccag 
     3541 gaccacccca gtcactgagc gctccagtgc ccaagtctac acaggccggc gaggggactc 
     3601 tgagtgaagc tctgctgcac ctgcagttcg acgctgatga ggacctggga gctctgctgg 
     3661 ggaacagcac cgatcccgga gtgttcacag atctggcctc cgtggacaac tctgagtttc 
     3721 agcagctgct gaatcagggc gtgtccatgt ctcatagtac agccgaacca atgctgatgg 
     3781 agtaccccga agccattacc cggctggtga ccggcagcca gcggcccccc gaccccgctc 
     3841 caactcccct gggaaccagc ggcctgccta atgggctgtc cggagatgaa gacttctcaa 
     3901 gcatcgctga tatggacttt agtgccctgc tgagtgggtc tggcagcggc agcgaccttt 
     3961 cccatccgcc cccaaggggc catctggatg agctgacaac cacacttgag tccatgaccg 
     4021 aggatctgaa cctggactca cccctgaccc cggaattgaa cgagattctg gataccttcc 
     4081 tgaacgacga gtgcctcttg catgccatgc atatcagcac aggactgtcc atcttcgaca 
     4141 catctctgtt tggatccaac ggtccgggtg gatctggagg tggaggttct ggaggacgaa 
     4201 tcatggccag aatcctggcc ttcgctatcg gcatcagcag catcggctgg gccttcagcg 
     4261 agaacgacga gctgaaggac tgcggcgtgc ggatcttcac caaggtggaa aaccccaaga 
     4321 ccggcgagag cctggccctg cccagaaggc tggccagaag cgcccggaag agactggcca 
     4381 gacggaaggc ccggctgaac cacctgaagc acctgatcgc caacgagttc aagctgaact 
     4441 acgaggacta ccagagcttc gacgagtccc tggccaaggc ctacaagggc agcctgatca 
     4501 gcccctacga gctgcggttc cgggccctga acgagctgct gagcaagcag gacttcgcca 
     4561 gagtgatcct gcacattgcc aagcggagag gctacgacga catcaagaac agcgacgaca 
     4621 aagagaaggg cgccatcctg aaggccatca agcagaacga ggaaaagctg gccaactacc 
     4681 agtccgtggg cgagtacctg tacaaagagt acttccagaa gttcaaagag aacagcaaag 
     4741 aattcaccaa cgtgcggaac aagaaagaaa gctacgagcg gtgtatcgcc cagagcttcc 
     4801 tgaaggatga gctgaagctg atcttcaaga agcagagaga gttcggcttc agcttcagca 
     4861 agaaattcga ggaagaggtg ctgagcgtcg ccttctacaa gagagccctg aaggacttca 
     4921 gccacctcgt gggcaactgc agcttcttca ccgacgagaa gagagccccc aagaacagcc 
     4981 ccctggcctt catgttcgtg gccctgaccc ggatcatcaa cctgctgaac aatctgaaga 
     5041 acaccgaggg catcctgtac accaaggacg acctgaacgc cctgctgaat gaggtgctga 
     5101 agaacggcac cctgacctac aagcagacca agaagctgct gggcctgagc gacgactacg 
     5161 agtttaaggg cgagaagggc acctacttca tcgagttcaa gaagtacaaa gagttcatca 
     5221 aggccctggg cgagcacaac ctgagccagg acgatctgaa tgagatcgcc aaggacatca 
     5281 ccctgatcaa ggacgagatt aagctgaaga aggccctggc caaatacgac ctgaatcaga 
     5341 accagatcga cagcctgagc aagctggaat tcaaggatca cctgaacatc agcttcaagg 
     5401 ctctgaagct ggtcaccccc ctgatgctgg aaggcaagaa gtacgacgag gcctgcaacg 
     5461 agctgaacct gaaggtggcc atcaacgagg acaagaagga cttcctgccc gccttcaacg 
     5521 aaacctacta caaggacgaa gtgaccaacc ccgtggtgct gcgggccatc aaagaatacc 
     5581 ggaaggtgct gaatgccctg ctcaagaaat acggcaaggt gcacaagatc aacatcgagc 
     5641 tggcccggga agtgggcaag aaccacagcc agcgggccaa gggaagcaag aacctgccca 
     5701 ccaagaaaca gaagagaatc ctggacaaga attacaagga caaagagcag aagaacttca 
     5761 aggaccggaa cctgaacgac acccggtata tcgcccggct ggtgctgaac tacacaaagg 
     5821 actacctgga tttcctgccc ctgtccgacg acgagaacac caagctgaac gatacccaga 
     5881 aaggctccaa ggtgcacgtg gaagccaaga gcggcatgct gaccagcgcc ctgagacaca 
     5941 cctggggctt cagcgccaag gatcggaaca accatctgca ccacgccatc gacgccgtga 
     6001 tcattgccta cgccaacaac agcatcgtga aggccttctc cgacttcaag aaagaacagg 
     6061 aaagcaacag cgccgagctg tacgccaaga agatctctga gctggactac aagaacaagc 
     6121 ggaagttctt cgagcccttc agcggcttcc ggcagaaggt gctggataag atcgacgaga 
     6181 tcttcgtgtc caagcccgag cggaagaagc cctctggcgc cctgcacgag gaaaccttca 
     6241 gaaaagagga agagttctac cagtcctacg gcggcaaaga aggcgtgctg aaggccctcg 
     6301 agctgggcaa gatcagaaaa gtgaacggca agatcgtgaa gaacggggac atgttccggg 
     6361 tggacatctt caagcacaaa aagaccaaca agttctacgc cgtgcccatc tacacaatgg 
     6421 acttcgccct gaaggtgctg cccaacaagg ccgtggcccg gtccaagaag ggcgagatca 
     6481 aggactggat tctgatggac gagaactacg agttctgctt tagcctgtac aaggactccc 
     6541 tgatcctgat ccagaccaag gacatgcagg aacccgagtt cgtctactac aacgccttca 
     6601 ccagcagcac cgtgtccctg atcgtgtcta agcacgacaa caagttcgag acactgagca 
     6661 agaaccagaa gatcctgttc aagaacgcca acgagaaaga agtgatcgcc aagagcatcg 
     6721 gcatccagaa tctgaaggtg ttcgagaagt acatcgtgtc cgccctggga gaagtgacaa 
     6781 aggccgagtt ccggcagaga gaggacttca aaaagtccgg acctccaaag aaaaagaga 
//
