LOCUS       pRGEN-CMV-VPR-CjCas9\WT        7355 bp    DNA     circular SYN 30-DEC-2018
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2017Äê5ÔÂ25ÈÕ from SnapGene Viewer 2.8.3 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12619751|
COMMENT     VNTDBDATE|-12619896|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRGEN-CMV-VPR-CjCas9 WT|
COMMENT     VNTAUTHORNAME|ZXX|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pRGEN-CMV-VPR-CjCas9 WT'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 12/30/18 03:26\317\302\316\347" 1) 
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COMMENT                          "Last Modified: 12/30/18 05:51\317\302\316\347" 1) 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                                      #242=(CLinePar 
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COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #243=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 119019944 (8 0) 1 1 1 0 178) 
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COMMENT                                     (CObjectList 
COMMENT                                      #246=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3239 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #247=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119020904 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HpaI: 0 sites") 3 3 "GTTAAC" 
COMMENT                            "CAATTG") 
COMMENT                      #248=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021512 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" 
COMMENT                            "CCATGG") 
COMMENT                      #249=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021576 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #250=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #27# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #251=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #27# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2511 " 1))))) "MfeI: 1 site") 
COMMENT                            1 5 "CAATTG" "GTTAAC") 
COMMENT                      #252=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021128 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #253=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #28# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #254=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #28# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2578 " 1) 
COMMENT                                      #255=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #28# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3296 " 1))))) "MluI: 2 sites") 
COMMENT                            1 5 "ACGCGT" "TGCGCA") 
COMMENT                      #256=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119020520 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NcoI: 4 sites") 1 5 "CCATGG" 
COMMENT                            "GGTACC") 
COMMENT                      #257=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119020968 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #258=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #29# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #259=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2834 " 1))))) "NdeI: 1 site") 
COMMENT                            2 4 "CATATG" "GTATAC") 
COMMENT                      #260=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021288 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #261=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021416 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #262=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #31# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #263=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #31# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3455 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #264=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021448 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                            "AATTAATT") 
COMMENT                      #265=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021736 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PmeI: 0 sites") 4 4 "GTTTAAAC" 
COMMENT                            "CAAATTTG") 
COMMENT                      #266=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021160 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #267=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #34# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #268=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #34# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6414 " 1))))) "PmlI: 1 site") 
COMMENT                            3 3 "CACGTG" "GTGCAC") 
COMMENT                      #269=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119020200 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #270=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #271=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3689 " 1) 
COMMENT                                      #272=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5119 " 1) 
COMMENT                                      #273=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6029 " 1))))) "PstI: 3 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #274=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119020808 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #275=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #35# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #276=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #35# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1797 " 1))))) "PvuI: 1 site") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #277=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021608 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #278=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #36# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #279=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 358 " 1) 
COMMENT                                      #280=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #36# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3789 " 1))))) "PvuII: 2 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #281=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021640 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #282=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #37# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #283=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4339 " 1))))) "RsrII: 1 site") 
COMMENT                            2 5 "CGGWCCG" "GCCWGGC") 
COMMENT                      #284=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021480 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #285=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #38# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #286=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 95 " 1) 
COMMENT                                      #287=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3168 " 1) 
COMMENT                                      #288=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3717 " 1))))) "SacI: 3 sites") 
COMMENT                            5 1 "GAGCTC" "CTCGAG") 
COMMENT                      #289=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021000 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SacII: 0 sites") 4 2 "CCGCGG" 
COMMENT                            "GGCGCC") 
COMMENT                      #290=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021032 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #291=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #40# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #292=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2348 " 1))))) "SalI: 1 site") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #293=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021704 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #294=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #41# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #295=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1907 " 1) 
COMMENT                                      #296=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4888 " 1))))) "ScaI: 2 sites") 
COMMENT                            3 3 "AGTACT" "TCATGA") 
COMMENT                      #297=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119021096 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SfiI: 0 sites") 8 5 
COMMENT                            "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
COMMENT                      #298=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119020296 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #299=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #300=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5824 " 1))))) "SmaI: 1 site") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #301=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119020776 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #302=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #43# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #303=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #43# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2940 " 1))))) "SnaBI: 1 site") 
COMMENT                            3 3 "TACGTA" "ATGCAT") 
COMMENT                      #304=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119022152 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #305=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #44# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #306=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #44# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2599 " 1))))) "SpeI: 1 site") 
COMMENT                            1 5 "ACTAGT" "TGATCA") 
COMMENT                      #307=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119022184 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SphI: 4 sites") 5 1 "GCATGC" 
COMMENT                            "CGTACG") 
COMMENT                      #308=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119022728 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "StuI: 4 sites") 3 3 "AGGCCT" 
COMMENT                            "TCCGGA") 
COMMENT                      #309=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119022760 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #310=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #47# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #311=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #47# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 53 " 1) 
COMMENT                                      #312=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #47# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3393 " 1))))) "XbaI: 2 sites") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
COMMENT                      #313=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119022216 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #314=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #48# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #315=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #48# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 41 " 1) 
COMMENT                                      #316=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #48# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6814 " 1))))) "XhoI: 2 sites") 
COMMENT                            1 5 "CTCGAG" "GAGCTC") 
COMMENT                      #317=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 119019976 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #318=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #319=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5822 " 1))))) "XmaI: 1 site") 
COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map:  (no more than 3 sites)") 
COMMENT              #320=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #321=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #322=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 1 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame 1 (0 ORFs)") 
COMMENT                              #323=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 2 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame 2 (0 ORFs)") 
COMMENT                              #324=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #325=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 62552768 (4 #74# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #326=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #327=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3123 - 33" 1))) 
COMMENT                                            "3123(d3) (1422 codons)"))) 
COMMENT                                    "Reading Frame 3 (1 ORF)"))) 
COMMENT                            "Direct(Forward) strand") 
COMMENT                      #328=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #329=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 -1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #330=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 62552824 (4 #75# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #331=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #332=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4119 - 6491" 
COMMENT                                                    1))) "4119(c1) (791 codons)"))) 
COMMENT                                    "Reading Frame 1 (1 ORF)") 
COMMENT                              #333=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 2 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame 2 (0 ORFs)") 
COMMENT                              #334=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 -3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #335=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 62552600 (4 #76# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #336=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #337=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4119 - 48" 1))) 
COMMENT                                            "4119(c3) (1095 codons)"))) 
COMMENT                                    "Reading Frame 3 (1 ORF)"))) 
COMMENT                            "Complementary(Reverse) strand"))) 
COMMENT                    "Open Reading Frames")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #338=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #339=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #340=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #341=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #342=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #343=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #344=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #345=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #346=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #347=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #348=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #349=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #350=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #351=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #352=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #353=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #354=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #355=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #356=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #357=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #358=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #359=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #360=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #361=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #362=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #363=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #364=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #365=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #366=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #367=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #368=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #369=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #370=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #371=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #372=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #373=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #374=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #375=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
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FEATURES             Location/Qualifiers
     CDS             7..33
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /vntifkey="4"
                     /label=HA
     promoter        complement(67..85)
                     /vntifkey="30"
                     /label=SP6\promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    111..335
                     /vntifkey="25"
                     /label=bGH\poly(A)\signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(594..1182)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1353..2213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2214..2318)
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     enhancer        2584..2963
                     /vntifkey="9"
                     /label=CMV\enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2964..3167
                     /vntifkey="30"
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter"
     promoter        3212..3230
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7341..6
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /vntifkey="4"
                     /label=SV40\NLS
     primer          3118..3138
                     /vntifkey="27"
                     /label=CMV-F
     primer          91..110
                     /vntifkey="27"
                     /label=poly\A-R
     CDS             4380..33
                     /vntifkey="4"
                     /label=CJ\cas9
     exon            6054..6056
                     /vntifkey="61"
                     /label=H559A
     exon            4401..4403
                     /vntifkey="61"
                     /label=D8A
     misc_feature    3255..4229
                     /vntifkey="21"
                     /label=VPR
     misc_feature    4230..4373
                     /vntifkey="21"
                     /label=LINKER
BASE COUNT     1956 a      1963 c      1942 g      1494 t 
ORIGIN
        1 aaagtatacc cctacgacgt gcccgactac gcctaataac tcgagcatgc atctagaggg 
       61 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc 
      121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
      181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
      241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
      301 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
      361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
      421 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
      481 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
      541 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
      601 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
      661 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
      721 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
      781 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
      841 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
      901 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
      961 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     1021 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     1081 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     1141 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     1201 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     1261 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     1321 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     1381 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     1441 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     1501 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     1561 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     1621 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     1681 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     1741 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     1801 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     1861 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     1921 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     1981 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     2041 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     2101 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     2161 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     2221 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     2281 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     2341 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct 
     2401 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 
     2461 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 
     2521 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc 
     2581 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
     2641 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
     2701 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     2761 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     2821 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     2881 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
     2941 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 
     3001 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 
     3061 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 
     3121 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta 
     3181 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag 
     3241 cttgggccac catggatgct ttagacgatt ttgacttaga tatgcttggt tcagacgcgt 
     3301 tagacgactt cgacctagac atgttaggct cagatgcatt ggacgacttc gatttagata 
     3361 tgttgggctc cgatgcccta gatgactttg atctagatat gctaggagga agcggaggag 
     3421 gaggtagcgg acctaagaaa aagaggaagg tggcggccgc tggatcccct tcagggcaga 
     3481 tcagcaacca ggccctggct ctggccccta gctccgctcc agtgctggcc cagactatgg 
     3541 tgccctctag tgctatggtg cctctggccc agccacctgc tccagcccct gtgctgaccc 
     3601 caggaccacc ccagtcactg agcgctccag tgcccaagtc tacacaggcc ggcgagggga 
     3661 ctctgagtga agctctgctg cacctgcagt tcgacgctga tgaggacctg ggagctctgc 
     3721 tggggaacag caccgatccc ggagtgttca cagatctggc ctccgtggac aactctgagt 
     3781 ttcagcagct gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa ccaatgctga 
     3841 tggagtaccc cgaagccatt acccggctgg tgaccggcag ccagcggccc cccgaccccg 
     3901 ctccaactcc cctgggaacc agcggcctgc ctaatgggct gtccggagat gaagacttct 
     3961 caagcatcgc tgatatggac tttagtgccc tgctgtcaca gatttcctct agtgggtctg 
     4021 gcagcggcag cgacctttcc catccgcccc caaggggcca tctggatgag ctgacaacca 
     4081 cacttgagtc catgaccgag gatctgaacc tggactcacc cctgaccccg gaattgaacg 
     4141 agattctgga taccttcctg aacgacgagt gcctcttgca tgccatgcat atcagcacag 
     4201 gactgtccat cttcgacaca tctctgttta gcctgggcag cggctccccc aagaaaaaac 
     4261 gcaaggtgga agatcctaag aaaaagcgga aagtggacgg cattggtagt gggagcaacg 
     4321 gcagcagcgg atccaacggt ccgggtggat ctggaggtgg aggttctgga ggacgaacca 
     4381 tggccagaat cctggccttc gacatcggca tcagcagcat cggctgggcc ttcagcgaga 
     4441 acgacgagct gaaggactgc ggcgtgcgga tcttcaccaa ggtggaaaac cccaagaccg 
     4501 gcgagagcct ggccctgccc agaaggctgg ccagaagcgc ccggaagaga ctggccagac 
     4561 ggaaggcccg gctgaaccac ctgaagcacc tgatcgccaa cgagttcaag ctgaactacg 
     4621 aggactacca gagcttcgac gagtccctgg ccaaggccta caagggcagc ctgatcagcc 
     4681 cctacgagct gcggttccgg gccctgaacg agctgctgag caagcaggac ttcgccagag 
     4741 tgatcctgca cattgccaag cggagaggct acgacgacat caagaacagc gacgacaaag 
     4801 agaagggcgc catcctgaag gccatcaagc agaacgagga aaagctggcc aactaccagt 
     4861 ccgtgggcga gtacctgtac aaagagtact tccagaagtt caaagagaac agcaaagaat 
     4921 tcaccaacgt gcggaacaag aaagaaagct acgagcggtg tatcgcccag agcttcctga 
     4981 aggatgagct gaagctgatc ttcaagaagc agagagagtt cggcttcagc ttcagcaaga 
     5041 aattcgagga agaggtgctg agcgtcgcct tctacaagag agccctgaag gacttcagcc 
     5101 acctcgtggg caactgcagc ttcttcaccg acgagaagag agcccccaag aacagccccc 
     5161 tggccttcat gttcgtggcc ctgacccgga tcatcaacct gctgaacaat ctgaagaaca 
     5221 ccgagggcat cctgtacacc aaggacgacc tgaacgccct gctgaatgag gtgctgaaga 
     5281 acggcaccct gacctacaag cagaccaaga agctgctggg cctgagcgac gactacgagt 
     5341 ttaagggcga gaagggcacc tacttcatcg agttcaagaa gtacaaagag ttcatcaagg 
     5401 ccctgggcga gcacaacctg agccaggacg atctgaatga gatcgccaag gacatcaccc 
     5461 tgatcaagga cgagattaag ctgaagaagg ccctggccaa atacgacctg aatcagaacc 
     5521 agatcgacag cctgagcaag ctggaattca aggatcacct gaacatcagc ttcaaggctc 
     5581 tgaagctggt cacccccctg atgctggaag gcaagaagta cgacgaggcc tgcaacgagc 
     5641 tgaacctgaa ggtggccatc aacgaggaca agaaggactt cctgcccgcc ttcaacgaaa 
     5701 cctactacaa ggacgaagtg accaaccccg tggtgctgcg ggccatcaaa gaataccgga 
     5761 aggtgctgaa tgccctgctc aagaaatacg gcaaggtgca caagatcaac atcgagctgg 
     5821 cccgggaagt gggcaagaac cacagccagc gggccaagat cgagaaagag cagaacgaaa 
     5881 actacaaggc caagaaggac gctgagctgg aatgcgagaa gctgggactg aagatcaaca 
     5941 gcaagaacat cctgaagctg cggctgttca aagaacagaa agagttctgc gcctacagcg 
     6001 gcgagaagat caagatcagc gatctgcagg acgagaagat gctggaaatc gaccacatct 
     6061 acccctacag ccggtccttc gacgacagct acatgaacaa ggtgctggtg ttcaccaaac 
     6121 agaaccagga aaaactgaac cagaccccct tcgaggcctt cggcaacgac agcgccaagt 
     6181 ggcagaaaat cgaggtgctg gccaagaacc tgcccaccaa gaaacagaag agaatcctgg 
     6241 acaagaatta caaggacaaa gagcagaaga acttcaagga ccggaacctg aacgacaccc 
     6301 ggtatatcgc ccggctggtg ctgaactaca caaaggacta cctggatttc ctgcccctgt 
     6361 ccgacgacga gaacaccaag ctgaacgata cccagaaagg ctccaaggtg cacgtggaag 
     6421 ccaagagcgg catgctgacc agcgccctga gacacacctg gggcttcagc gccaaggatc 
     6481 ggaacaacca tctgcaccac gccatcgacg ccgtgatcat tgcctacgcc aacaacagca 
     6541 tcgtgaaggc cttctccgac ttcaagaaag aacaggaaag caacagcgcc gagctgtacg 
     6601 ccaagaagat ctctgagctg gactacaaga acaagcggaa gttcttcgag cccttcagcg 
     6661 gcttccggca gaaggtgctg gataagatcg acgagatctt cgtgtccaag cccgagcgga 
     6721 agaagccctc tggcgccctg cacgaggaaa ccttcagaaa agaggaagag ttctaccagt 
     6781 cctacggcgg caaagaaggc gtgctgaagg ccctcgagct gggcaagatc agaaaagtga 
     6841 acggcaagat cgtgaagaac ggggacatgt tccgggtgga catcttcaag cacaaaaaga 
     6901 ccaacaagtt ctacgccgtg cccatctaca ccatggactt cgccctgaag gtgctgccca 
     6961 acaaggccgt ggcccggtcc aagaagggcg agatcaagga ctggattctg atggacgaga 
     7021 actacgagtt ctgctttagc ctgtacaagg actccctgat cctgatccag accaaggaca 
     7081 tgcaggaacc cgagttcgtc tactacaacg ccttcaccag cagcaccgtg tccctgatcg 
     7141 tgtctaagca cgacaacaag ttcgagacac tgagcaagaa ccagaagatc ctgttcaaga 
     7201 acgccaacga gaaagaagtg atcgccaaga gcatcggcat ccagaatctg aaggtgttcg 
     7261 agaagtacat cgtgtccgcc ctgggagaag tgacaaaggc cgagttccgg cagagagagg 
     7321 acttcaaaaa gtccggacct ccaaagaaaa agaga 
//
