LOCUS       pU6-cj-EsgRNA        3376 bp    DNA     circular SYN 16-DEC-2018
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2017Äê6ÔÂ28ÈÕ from SnapGene Viewer 2.8.3 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12592380|
COMMENT     VNTDBDATE|-12599867|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pU6-cj-EsgRNA|
COMMENT     VNTAUTHORNAME|ZXX|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                            4 2 "CGATCG" "GCTAGC") 
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COMMENT                    "Restriction/Methylation Map:  (no less than 1 site) and (no more than 3 sites)")))) 
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FEATURES             Location/Qualifiers
     protein_bind    519..540
                     /bound_moiety="E. coli catabolite activator protein"
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding activates transcription in the presence of cAMP."
     promoter        555..585
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=lac\operator
                     /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     617..633
                     /vntifkey="28"
                     /label=M13\rev
                     /note="common sequencing primer, one of multiple similar variants"
     promoter        651..669
                     /vntifkey="30"
                     /label=SP6\promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             complement(693..719)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /vntifkey="4"
                     /label=HA
     promoter        complement(1168..1186)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1193..1209)
                     /vntifkey="28"
                     /label=M13\fwd
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      1350..1804
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        1883..1987
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     CDS             1988..2848
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     rep_origin      3019..231
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     exon            835..1083
                     /vntifkey="61"
                     /label=u6
     misc_feature    734..813
                     /vntifkey="21"
                     /label=E\sg
     misc_feature    809..809
                     /vntifkey="21"
                     /label=t-a
     misc_feature    790..790
                     /vntifkey="21"
                     /label=a-t
     misc_feature    714..789
                     /vntifkey="21"
                     /label=F
     misc_feature    770..845
                     /vntifkey="21"
                     /label=R
BASE COUNT      855 a       830 c       794 g       897 t 
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 
      601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga 
      661 cactatagaa tactcaagcg tcctcccagg ctggcatagt caggcacgtc ataaggatag 
      721 aattcgatgt cgaaaaaaaa gcggttttag gggattgtaa ccccgcagag tcccgcaaac 
      781 tctttattta agtccctttt cagggactta aacggagacg ggatcccgtc tcccggtgtt 
      841 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag 
      901 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta 
      961 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattccaat 
     1021 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc 
     1081 ctcggtgaat ctagactcga gatcgaattc cagatcctct tctgagagag tttttgttca 
     1141 tcgtcgtcat ccttgtaatc aattcgccct atagtgagtc gtattacaat tcactggccg 
     1201 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     1261 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     1321 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc 
     1381 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     1441 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     1501 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     1561 tgattagagt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     1621 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     1681 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     1741 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 
     1801 aattccctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg 
     1861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     1921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     1981 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 
     2041 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 
     2101 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 
     2161 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 
     2221 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 
     2281 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
     2341 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 
     2401 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 
     2461 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 
     2521 cacgatgcct gtagcaatgg caacaacgtt gcacaaacta ttaactggcg aactacttac 
     2581 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
     2641 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 
     2701 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 
     2761 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 
     2821 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 
     2881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
     2941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
     3001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     3061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     3121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     3181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     3241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     3301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     3361 cagcttggag cgaacg 
//
