LOCUS       pKB224                  6973 bp ds-DNA     circular     27-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        384..402
                     /label="pSC101* promoter (-)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    746..1113
                     /label="par region"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1119..1224)
                     /label="T0"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(1253..1915)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(1253..1915)
                     /label="Translation 1915-1253"
     misc_feature    1391..1394
                     /label="BsaI out"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2240..2272
                     /label="SR224"
                     /note="sequence: GCATTAGGTCTCGCGTCTAAGAAACCATTATTATCATGACATTAAC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2306..2375
                     /label="PT0031"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2376..2495
                     /label="PvirK"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2382..2398
                     /label="PhoP Box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2425..2441
                     /label="PhoP Box"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2432..2437
                     /label="-35"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2454..2459
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2496..2513
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          2514..2534
                     /label="RB140"
                     /note="sequence: aggtctctTCTAATGGTTTCGAAAGGTGAGGAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2514..2535
                     /label="KB625"
                     /note="sequence: aggtctcaATGGTTTCGAAAGGTGAGGAGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2514..3224
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             2514..3224
                     /label="Translation 2514-3224"
     primer          2966..2992
                     /label="kg904"
                     /note="sequence: CAAAAAGACGTATCCCAATGATAAAAC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(3201..3224)
                     /label="RB141"
                     /note="sequence: aggtctctCTGGTCATTTGTACAGTTCATCCATGCC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3225..3353
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer          complement(3331..3357)
                     /label="KB340"
                     /note="sequence: aggtctcaaaacTATAAACGCAGAAAGGCCCACCC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          3354..3378
                     /label="KB341"
                     /note="sequence: AGGTCTCAgtttcacttctgagttcggcatggg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(3354..3413)
                     /label="rrnB terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3395..3413)
                     /label="KB589"
                     /note="sequence: aggtctcatgcctggcggcagtagcgc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3410..3460
                     /label="KB615"
                     /note="sequence: tggtctctggcaGCTGTATCATCTGCGTACTCCATTAGTGTGACGACCTATGAGGATGG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3414..3438)
                     /label="primer binding site"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3439..3711
                     /label="mNeptune 2.5"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(3619..3636)
                     /label="KB616"
                     /note="sequence: aggtctcactaaAACGACCCTCAAGACCGC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          3638..3661
                     /label="KB613"
                     /note="sequence: TGATATGGCGTTGAAACTGGTCGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(3662..3686)
                     /label="KB619"
                     /note="sequence: aggtctcaCTAAgactcagCAGATTACAATGCAAATGTCCACCG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3684..3715)
                     /label="KB618"
                     /note="sequence: aggtctcactaaGCTTCTTGCTACGATAAGTCGTTTTCAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(3712..3714)
                     /label="Translation 3714-3712"
     primer          3712..3741
                     /label="KB621"
                     /note="sequence: aggtctcaTTAgCTCAGCTTCTGATTTAAGTCCTCAGG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3715..3750)
                     /label="NC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(3715..3750)
                     /label="Translation 3750-3715"
     misc_feature    complement(3751..3759)
                     /label="extra linker"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3751..4446)
                     /label="Translation 4446-3751"
     primer          3752..3770
                     /label="KB554"
                     /note="sequence: aggtctcagttcctccgatacccgcag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(3754..3772)
                     /label="KB585"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctgcgggtatcggagga"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(3754..3776)
                     /label="Primer P5 FWD binding site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(3754..3776)
                     /label="KB571"
                     /note="sequence: ctccagctgcgggtatcggagga"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(3760..3999)
                     /label="nr-2X_tether"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(4012..4353)
                     /label="OmpA 46-159"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(4360..4446)
                     /label="lpp + 9aa"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(4419..4447)
                     /label="KB390"
                     /note="sequence: aggtctcatctgatgaaagctactaaactggtactgggcg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4447..4531)
                     /label="apFAB677 (21.45)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(4532..4599)
                     /label="Ptac"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(4557..4557)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4559..4559)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4566..4571)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4588..4593)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4599..4623)
                     /label="Sequencing primer rev SR1266"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(4708..4728)
                     /label="KD350"
                     /note="sequence: CGGTATCAGCTCACTCAAAGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4715..4732)
                     /label="Sequencing primer fw SR1265"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      complement(4753..4857)
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    complement(4858..5940)
                     /label="lacI"
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_fwdcolor=#408080
     misc_feature    complement(5084..5084)
                     /label="C to T variation"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(5941..6040)
                     /label="PlacIq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(5968..5968)
                     /label="+1 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(5976..5981)
                     /label="-10 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(5999..6004)
                     /label="-35 region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          6041..6060
                     /label="KB339"
                     /note="sequence: aggtctcaGCAAGGTGTCACCACCCTGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      join(6385..6973,1..363)
                     /label="pSC101* (-)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 GGAAAAGATA AGGCAATATC TCTTCACTAA AAACTAATTC TAATTTTTCG CTTGAGAACT
       61 TGGCATAGTT TGTCCACTGG AAAATCTCAA AGCCTTTAAC CAAAGGATTC CTGATTTCCA
      121 CAGTTCTCGT CATCAGCTCT CTGGTTGCTT TAGCTAATAC ACCATAAGCA TTTTCCCTAC
      181 TGATGTTCAT CATCTGAGCG TATTGGTTAT AAGTGAACGA TACCGTCCGT TCTTTCCTTG
      241 TAGGGTTTTC AATCGTGGGG TTGAGTAGTG CCACACAGCA TAAAATTAGC TTGGTTTCAT
      301 GCTCCGTTAA GTCATAGCGA CTAATCGCTA GTTCATTTGC TTTGAAAACA ACTAATTCAG
      361 ACATACATCT CAATTGGTCT Aggtgatttt aatcactata ccaattgaga tgggctagtc
      421 aatgataatt acatgtccTT TTCCTTTGAG TTGTGGGTAT CTGTAAATTC TGCTAGACCT
      481 TTGCTGGAAA ACTTGTAAAT TCTGCTAGAC CCTCTGTAAA TTCCGCTAGA CCTTTGTGTG
      541 TTTTTTTTGT TTATATTCAA GTGGTTATAA TTTATAGAAT AAAGAAAGAA TAAAAAAAGA
      601 TAAAAAGAAT AGATCCCAGC CCTGTGTATA ACTCACTACT TTAGTCAGTT CCGCAGTATT
      661 ACAAAAGGAT GTCGCAAACG CTGTTTGCTC CTCTACAAAA CAGACCTTAA AACCCTAAAG
      721 GCTTAAGTAG CACCCTCGCA AGCTCGGGCA AATCGCTGAA TATTCCTTTT GTCTCCGACC
      781 ATCAGGCACC TGAGTCGCTG TCTTTTTCGT GACATTCAGT TCGCTGCGCT CACGGCTCTG
      841 GCAGTGAATG GGGGTAAATG GCACTACAGG CGCCTTTTAT GGATTCATGC AAGGAAACTA
      901 CCCATAATAC AAGAAAAGCC CGTCACGGGC TTCTCAGGGC GTTTTATGGC GGGTCTGCTA
      961 TGTGGTGCTA TCTGACTTTT TGCTGTTCAG CAGTTCCTGC CCTCTGATTT TCCAGTCTGA
     1021 CCACTTCGGA TTATCCCGTG ACAGGTCATT CAGACTGGCT AATGCACCCA GTAAGGCAGC
     1081 GGTATCATCA ACAGGCTTAC CCGTCTTACT GTCCCTAGTG CTTGGATTCT CACCAATAAA
     1141 AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG AGGTCATTAC
     1201 TGGATCTATC AACAGGAGTC CAAGCGAGCT CGTAAACTTG GTCTGACAGT TACCAATGCT
     1261 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
     1321 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
     1381 TGATACCGCG TGATCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
     1441 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
     1501 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
     1561 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
     1621 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
     1681 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
     1741 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
     1801 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
     1861 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
     1921 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
     1981 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
     2041 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
     2101 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
     2161 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
     2221 TTCCCATGGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA
     2281 ATAGGCGTAT CACGAGGCAG AATTTttatc gcttccgcgt tccgtctact ccctattacg
     2341 ctccggaatc tccgacgcct acggccgacg tcggataccg ccattgataa actgtttaac
     2401 aacatcgtct gtacagacct tcttcgttgc ctttacgttt aactcaatca ggctaccgtc
     2461 tcggttataa gtctatcgag tagtagagcc gtagtAAAGA GGAGAAATAC TAGATGGTTT
     2521 CGAAAGGTGA GGAGGATAAC ATGGCGTCGC TGCCTGCTAC ACATGAACTG CATATTTTCG
     2581 GCAGCATCAA CGGAGTTGAT TTCGACATGG TGGGCCAAGG CACCGGCAAC CCGAACGACG
     2641 GCTATGAAGA ATTGAATTTG AAATCTACCA AGGGCGACTT GCAATTTAGT CCGTGGATTC
     2701 TGGTACCGCA TATTGGTTAT GGTTTTCATC AGTATCTGCC CTACCCAGAC GGAATGAGCC
     2761 CATTTCAGGC GGCTATGGTG GATGGCAGTG GCTATCAGGT CCATCGCACC ATGCAATTCG
     2821 AGGATGGGGC GAGCCTGACC GTGAATTATC GGTACACTTA CGAAGGTTCA CATATTAAAG
     2881 GTGAAGCACA AGTTAAAGGC ACCGGCTTCC CGGCGGATGG CCCAGTGATG ACCAACAGCC
     2941 TGACGGCGGC GGACTGGTGT CGTAGCAAAA AGACGTATCC CAATGATAAA ACAATTATTT
     3001 CTACCTTTAA ATGGAGTTAC ACCACGGGTA ACGGTAAACG CTACCGTAGC ACGGCTCGCA
     3061 CCACATACAC CTTTGCGAAA CCCATGGCCG CGAACTATCT GAAAAATCAG CCGATGTATG
     3121 TGTTTCGCAA AACGGAACTG AAACATTCGA AAACTGAACT CAATTTCAAA GAGTGGCAAA
     3181 AAGCCTTCAC CGACGTAATG GGCATGGATG AACTGTACAA ATGACCAGGC ATCAAATAAA
     3241 ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC
     3301 TCTCTACTAG AGTCACACTG GCTCACCTTC GGGTGGGCCT TTCTGCGTTT ATAgtttcac
     3361 ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaGCTGTAT
     3421 CATCTGCGTA CTCCATTAGT GTGACGACCT ATGAGGATGG CGGGGTATTA ACTGTAACGC
     3481 AAGATACTTC TCTGCAAGAT GGTTGTTTAA TTTATAATGT GAAACTGCGC GGCGTTAATT
     3541 TTCCCTCAAA TGGTCCCGTA ATGCAAAAGA AGACCTTAGG GTGGGAAGCT TCTACGGAAA
     3601 CGTTGTATCC TGCAGATGGC GGTCTTGAGG GTCGTTGTGA TATGGCGTTG AAACTGGTCG
     3661 GCGGTGGACA TTTGCATTGT AATCTGAAAA CGACTTATCG TAGCAAGAAG CTTAgCTCAG
     3721 CTTCTGATTT AAGTCCTCAG GAGTAGCCCC ggttcctccg atacccgcag ctggagcctc
     3781 agcggcatct gcaggagcgg cttccgcagc cggtgcttca ctcgctggtg cctccgctgc
     3841 tggtgtagct tccgcagctg gtgcagcagg ttcctgactg atgccagcgg ctggggcttc
     3901 ggcagcatcc gctggggcag cttctgctgc aggcgcttcg ctagctggag cttcagcagc
     3961 aggagtcgcc tctgcggcgg gcgctgcagg ctcttgactg gtaccaatac cgttgtccgg
     4021 acgagtgccg atggtgtgtg cgtcaccgat gttgttggtc cactggtatt ccagacgggt
     4081 agcgatttca ggagtgatcg cgtactcaac accgccagcg aagaccggag aaacgccggt
     4141 gtcgtggttt ttaccataaa cgttggattt agtgtctgca cgccatacca tgccacccag
     4201 acgagtgtag atgtccaggt cgtcagtgat tgggtaaccc agtttagcgg tcagttgaac
     4261 gccctgagct ttgtatgcac cgttttcaac gctgcctttg tacggcatac gacctaacca
     4321 gtcgtaaccc atttcaaagc caacatacgg gttaattccc tgatcgattt tagcgttgct
     4381 ggagcaacct gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc
     4441 tttcatCAGA AATACTCCAT AGCATGATTA AGATGTTTCA GTACGAAAAT TGCTTTCATT
     4501 GTTGATCTCC TTTTTAAGTG AACTTGGGCC CTGTGTGAAA TTGTTATCCG CTCACAATTC
     4561 CACACATTAT ACGAGCCGAT GATTAATTGT CAACAGCTCA TTTCAGAATA TTAGCCAGAA
     4621 CCGTTATGAT GTCGGCGCAA AAAACATTAT CCAGAACGGG AGTGCGCCTT GAGCGACACG
     4681 AATTATGCAG TGATTTACGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
     4741 GAACGCCCTA GGTCTAGGGC GGCGGATTTG TCCTACTCAG GAGAGCGTTC ACCGACAAAC
     4801 AACAGATAAA ACGAAAGGCC CAGTCTTTCG ACTGAGCCTT TCGTTTTATT TGATGCCTCA
     4861 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC
     4921 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC
     4981 GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC
     5041 GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA
     5101 TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC
     5161 GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC
     5221 AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT
     5281 CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA
     5341 GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG
     5401 CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA
     5461 AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT
     5521 GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC
     5581 ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG
     5641 TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC
     5701 GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA
     5761 CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC
     5821 CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA
     5881 AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC
     5941 ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT
     6001 GCACCATTCG ATGGTGTCAA CGTAAATGCA TGCCGCTTCG GCAAGGTGTC ACCACCCTGC
     6061 CCTTTTTCTT TAAAACCGAA AAGATTACTT CGCGTTATGC AGGCTTCCTC GCTCACTGAC
     6121 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATC
     6181 TCGAGGTTAC ATTGTCGATC TGTTCATGGT GAACAGCTTT AAATGCACCA AAAACTCGTA
     6241 AAAGCTCTGA TGTATCTATC TTTTTTACAC CGTTTTCATC TGTGCATATG GACAGTTTTC
     6301 CCTTTGATAT CTAACGGTGA ACAGTTGTTC TACTTTTGTT TGTTAGTCTT GATGCTTCAC
     6361 TGATAGATAC AAGAGCCATA AGAACCTCAG ATCCTTCCGT ATTTAGCCAG TATGTTCTCT
     6421 AGTGTGGTTC GTTGTTTTTG CGTGAGCCAT GAGAACGAAC CATTGAGATC ATGCTTACTT
     6481 TGCATGTCAC TCAAAAATTT TGCCTCAAAA CTGGTGAGCT GAATTTTTGC AGTTAAAGCA
     6541 TCGTGTAGTG TTTTTCTTAG TCCGTTACGT AGGTAGGAAT CTGATGTAAT GGTTGTTGGT
     6601 ATTTTGTCAC CATTCATTTT TATCTGGTTG TTCTCAAGTT CGGTTACGAG ATCCATTTGT
     6661 CTATCTAGTT CAACTTGGAA AATCAACGTA TCAGTCGGGC GGCCTCGCTT ATCAACCACC
     6721 AATTTCATAT TGCTGTAAGT GTTTAAATCT TTACTTATTG GTTTCAAAAC CCATTGGTTA
     6781 AGCCTTTTAA ACTCATGGTA GTTATTTTCA AGCATTAACA TGAACTTAAA TTCATCAAGG
     6841 CTAATCTCTA TATTTGCCTT GTGAGTTTTC TTTTGTGTTA GTTCTTTTAA TAACCACTCA
     6901 TAAATCCTCA TAGAGTATTT GTTTTCAAAA GACTTAACAT GTTCCAGATT ATATTTTATG
     6961 AATTTTTTTA ACT
//