LOCUS       pUC19                   4209 bp    DNA     circular     05-APR-2021
FEATURES             Location/Qualifiers
     PCR_primer      complement(1988..2004)
                     /note="M13Rv"
     PCR_primer      359..375
                     /note="M13Fw"
     gene            complement(3149..4009)
                     /note="ApR"
     gene            753..1946
                     /note="Giles Integrase"
     promoter        614..752
                     /note=""
     PCR_primer      357..376
                     /note="pUC19_seq_Fw"
     PCR_primer      complement(2214..2234)
                     /note="pUC19_seq_Rv"
     source          1..4209
                     /dnas_title="pUC19 Giles integrase"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 ccTCTAGAca cggcctgtcc gcaccgcgga tcaggcgttg acgatgacgg gctggtcggc
      481 cacgtcgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
      541 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
      601 tttcgttgtt aacggaatga tgatcgccgc ggtgacacaa tcggcgtacg cggacgcccg
      661 gacgtgaccg gaggcgaccg tacacctatc ccgaccgggc actattcggc gtggatcccc
      721 acgcacccgg cgacccccga gaggacaccg caatggggcg acgcggcaac cgcgaaggca
      781 cataccggca ccggccggac gggcgctggg aggcccggtt gctctatgac gacccggtca
      841 ccggtgaacg ccgccgggca tcgttctacg gcaagacgaa gacggcggta cgggccaaaa
      901 tgcgcgaagc tatcgaccgc ctcgacgccg acgccccggt gcgtgacgcc gcggtgaccg
      961 tggccgattg gatgcgccgg tggtgcacca ccaccctgtt ggtctccggc cgcgccgaca
     1021 gcaccaaatc gttgtacggc acactgtcgc gccaatacgt cgaacccgcc ccgttcggcg
     1081 cgatccggtt ggaccgcctg cgcacatccg atgtcgaggc gttggtgctg cgccttcaac
     1141 aggccacccg cccgggccgc gacgggacgc cggtgcgcgc ggtcgccgac agcacgatcc
     1201 ggtcgaccta caccgtgttg cgccaggcgc tcgacggcgc ggtgcgcgac ggcctgatgg
     1261 cgcgcaaccc tgcggcggcc atgaagcggc ccgggttgtc gcgcccggaa gtccggcgga
     1321 tcaacgacgc gcagttggcc gacctgttgg ccgccaccgc cggatcccgc taccaccgcg
     1381 ccctgttgct gatcgcggcg accggactgc gcgccgggga ggcgttggcc cttcggtggg
     1441 acgccgtcga cctcgacgcc gggtcgctgc gcgtggtggc caccgtcggc cgcgtcgagc
     1501 gtcgcctgcg cctcacgacg ccgaagacgg aacgatcccg ccggacggtg ccgttgacgc
     1561 cggacatggt gtcgatgctg cgtcggcacc gcgccgacca gttagctgaa cgcctgcgcg
     1621 cggccaacgt gtgggaggac cacgggttgg tgttcccgtc ggcgttcggc cgcccggtgg
     1681 atccgcgcaa cctgttgcgg gtggtggagc aggccgccgc gcaggttggc ctcgagggcg
     1741 tcggcgtgca caccctgcgt cacgccgcgg cggtggcgat gctcgaggcg ggcatccaca
     1801 tccgcgcggt cgccgacatc ctcgggcatt cgtcggtggc ggtcacgggg gacatctacg
     1861 gccacacgtc cgacgcgacc gcgcgggccg ccgtcgacgt gttgtcggcg cgactgcgga
     1921 tcgcggacgg cagcggacag tcttagggta cccgataccg tcggcttccA AGCTTggcgt
     1981 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2041 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2101 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2161 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2221 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2281 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2581 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2821 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2941 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3061 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3121 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3181 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3241 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3301 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3361 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3421 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3481 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3541 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3601 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3661 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3721 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3781 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3841 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3901 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3961 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4021 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4081 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4141 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4201 cctttcgtc
//

