LOCUS       Plasmid                 4658 bp    DNA     circular     05-APR-2021
FEATURES             Location/Qualifiers
     gene            780..1595
                     /note="KanR"
     rep_origin      1859..2259
                     /note="oriE"
     PCR_primer      2315..2334
                     /note="pMV361Seq_Fw2"
                     /current=0
     PCR_primer      complement(4604..4623)
                     /note="pMV361Seq_Rv3"
     PCR_primer      2616..2635
                     /note="pMV361Seq_Fw3"
     PCR_primer      complement(763..782)
                     /note="pMV361_AflII_Rv"
     PCR_primer      1596..1616
                     /note="pMV361_PacI_Fw"
     PCR_primer      complement(1862..1881)
                     /note="pMV306_Kan_Rv1"
     PCR_primer      complement(1798..1817)
                     /note="pMV306_Kan_Rv2"
     PCR_primer      complement(9..28)
                     /note="pMV306 seq Rv5"
     PCR_primer      complement(493..510)
                     /note="pMV306 delint BamHI Rv"
     PCR_primer      511..534
                     /note="pMV306 delint BamHI Fw"
     gene            3068..3772
                     /note="mTagBFP2"
     PCR_primer      3074..3091
                     /note="mTagBFP2NcoIFw"
     promoter        2864..2927
                     /note="Psmyc core"
     PCR_primer      2927..2944
                     /note="pML1357_seq_Fw"
     RBS             3016..3021
                     /note="RBS1"
     PCR_primer      complement(3747..3765)
                     /note="mTagBFP2HindIIIRv"
     RBS             3788..3793
                     /note="RBS1"
     gene            3840..4544
                     /note="COBFP"
     source          1..4658
                     /dnas_title="Plasmid L5 attBPsmyc double blue"
ORIGIN      
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag GGATCCtccc aaggacactg agtcctaaag
      541 aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg
      601 ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca
      661 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
      721 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      781 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      841 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      901 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      961 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     1021 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     1081 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1141 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1201 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     1261 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     1321 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1381 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1441 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     1501 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1561 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     1621 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1681 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1741 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1801 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1861 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2581 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2641 gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtaccag atctttaaaT
     2701 CTAGAggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg acgtgatgat
     2761 cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc
     2821 gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa cccacccggg
     2881 gtgggcgagt ttgtcctgcg tgtgctcggt cgagtaggct ctgggagtac ccgtgtgtac
     2941 gaccagcacg gcatacatca tttcgacgcc gagagattcg ccgcccgaaa tgagcacgat
     3001 ccgcatgctt aattaaGGAG GTATCTCCAT GAGCGGTCAC CATCACCATC ACCACGGCGG
     3061 TAGCGCCATG GGCAGCGAGC TGATTAAGGA GAACATGCAC ATGAAGCTGT ACATGGAGGG
     3121 CACCGTGGAC AACCATCACT TCAAGTGCAC ATCCGAGGGC GAAGGCAAGC CCTACGAGGG
     3181 CACCCAGACC ATGAGAATCA AGGTGGTCGA GGGCGGCCCT CTCCCCTTCG CCTTCGACAT
     3241 CCTGGCTACT AGCTTCCTCT ACGGCAGCAA GACCTTCATC AACCACACCC AGGGCATCCC
     3301 CGACTTCTTC AAGCAGTCCT TCCCTGAGGG CTTCACATGG GAGAGAGTCA CCACATACGA
     3361 AGACGGGGGC GTGCTGACCG CTACCCAGGA CACCAGCCTC CAGGACGGCT GCCTCATCTA
     3421 CAACGTCAAG ATCAGAGGGG TGAACTTCAC ATCCAACGGC CCTGTGATGC AGAAGAAAAC
     3481 ACTCGGCTGG GAGGCCTTCA CCGAGACGCT GTACCCCGCT GACGGCGGCC TGGAAGGCAG
     3541 AAACGACATG GCCCTGAAGC TCGTGGGCGG GAGCCATCTG ATCGCAAACG CCAAGACCAC
     3601 ATATAGATCC AAGAAACCCG CTAAGAACCT CAAGATGCCT GGCGTCTACT ATGTGGACTA
     3661 CAGACTGGAA AGAATCAAGG AGGCCAACAA CGAGACCTAC GTCGAGCAGC ACGAGGTGGC
     3721 AGTGGCCAGA TACTGCGACC TCCCTAGCAA ACTGGGGCAC AAGCTCAATT AAaagcttgc
     3781 ttaattaaGG AGGTATCTCC ATGAGCGGTC ACCATCACCA TCACCACGGC GGTAGCGCCA
     3841 TGAGCTCGGA GCTGATCAAG GAGAACATGC ACATGAAGCT GTACATGGAG GGCACCGTGG
     3901 ACAACCACCA CTTCAAGTGC ACCTCGGAGG GCGAGGGCAA GCCGTACGAG GGCACCCAGA
     3961 CCATGCGCAT CAAGGTGGTG GAGGGCGGCC CGCTGCCGTT CGCCTTCGAC ATCCTGGCCA
     4021 CCTCGTTCCT GTACGGCTCG AAGACCTTCA TCAACCACAC CCAGGGCATC CCGGACTTCT
     4081 TCAAGCAGTC GTTCCCGGAG GGCTTCACCT GGGAGCGCGT GACCACCTAC GAGGACGGCG
     4141 GCGTGCTGAC CGCCACCCAG GACACCTCGC TGCAGGACGG CTGCCTGATC TACAACGTGA
     4201 AGATCCGCGG CGTGAACTTC ACCTCGAACG GCCCGGTGAT GCAGAAGAAG ACCCTGGGCT
     4261 GGGAGGCCTT CACCGAGACC CTGTACCCGG CCGACGGCGG CCTGGAGGGC CGCAACGACA
     4321 TGGCCCTGAA GCTGGTGGGC GGCTCGCACC TGATCGCCAA CGCCAAGACC ACCTACCGCT
     4381 CGAAGAAGCC GGCCAAGAAC CTGAAGATGC CGGGCGTGTA CTACGTGGAC TACCGCCTGG
     4441 AGCGCATCAA GGAGGCCAAC AACGAGACCT ACGTGGAGCA GCACGAGGTG GCCGTGGCCC
     4501 GCTACTGCGA CCTGCCGTCG AAGCTGGGCC ACAAGCTGAA CTAAtcgatg tcgacgtagt
     4561 taactagcgt acgatcgact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     4621 gggcctttcg ttttatgcca tcatggccgc ggtgatca
//

