LOCUS       pEc-crRNA1              1852 bp ds-DNA     circular     06-APR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(161..265)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(266..1029)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    341..930
                     /label="pBR322 ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1052..1086
                     /label="J23119"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1063..1087)
                     /label="FW567 change repeat 1 rev"
                     /note="sequence: cgctagcattatacctaggactgag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1087..1115
                     /label="E. coli I-E repeat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1116..1142
                     /label="FW566 change repeat 1 for"
                     /note="sequence: AATTCTGGCGAATCCTTTAATTAACTG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1116..1147
                     /label="E. coli I-E crRNA1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1123..1147)
                     /label="FW569 change repeat 2 rev"
                     /note="sequence: CTGGTCAGTTAATTAAAGGATTCGC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1148..1176
                     /label="E. coli I-E repeat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1174..1192
                     /label="FW568 change repeat 2 for"
                     /note="sequence: CCGaaaaaaaaaccccgcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1177..1215
                     /label="Terminator"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    complement(1244..1352)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(join(1353..1852,1..160))
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
       61 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      121 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      181 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      241 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
      301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1021 gataaccgtC GAGAGCATCG ATCCTAGCAT Gttgacagct agctcagtcc taggtataat
     1081 gctagcGAGT TCCCCGCGCC AGCGGGGATA AACCGAATTC TGGCGAATCC TTTAATTAAC
     1141 TGACCAGGAG TTCCCCGCGC CAGCGGGGAT AAACCGaaaa aaaaaccccg cccctgacag
     1201 ggcggggttt tttttAAAGA ATCGATCGAT CCCGAATGAG TAGaccaata aaaaacgccc
     1261 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1321 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1381 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1441 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1501 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1561 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1621 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1681 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1741 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1801 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa ct
//