LOCUS       pT7RNAP                 6296 bp ds-DNA     circular     06-APR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    231..1043
                     /label="Kan coding sequence"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1139..1594)
                     /label="f1 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1471..1490
                     /label="pr-07"
                     /note="sequence: aacgtggcgagaaaggaagg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1677..1731
                     /label="OR2-OR1-Pr"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1773..4424
                     /label="T7RNAP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4681..4710
                     /label="T500"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer          complement(4806..4825)
                     /label="pr-08"
                     /note="sequence: cgctcgcgtatcggtgattc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    join(5802..6296,1..125)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(5818..5818)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
       61 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      121 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      181 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
      241 ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat
      301 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
      361 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      421 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
      481 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
      541 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      601 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
      661 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
      721 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
      781 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
      841 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
      901 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
      961 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     1021 atttgatgct cgatgagttt ttctaagaat taattcatga gcggatacat atttgaatgt
     1081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
     1141 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     1201 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     1261 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     1321 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     1381 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     1441 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     1501 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     1561 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccaatccg gatatagttA
     1621 CATGTGTGTG CTGTTCCGCT GGGCATGCCA GGACAACTTC TGGTCCGGTA ACGTGCTGAG
     1681 CTAACACCGT GCGTGTTGAC AATTTTACCT CTGGCGGTGA TAATGGTTGC AGCTAGCAAT
     1741 AATTTTGTTT AACTTTAAGA AGGAGGATCC AAatgaacac gattaacatc gctaagaacg
     1801 acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac cattacggtg
     1861 agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg ggtgaagcac
     1921 gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat aacgctgccg
     1981 ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg
     2041 aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg caagaaatca
     2101 agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg
     2161 acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc
     2221 gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac
     2281 tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca
     2341 tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta
     2401 ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac
     2461 accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat
     2521 acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac
     2581 cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg
     2641 gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag
     2701 acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa
     2761 tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg
     2821 aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga
     2881 atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg
     2941 ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta
     3001 accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt
     3061 caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta
     3121 aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg
     3181 tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca
     3241 tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct
     3301 gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact
     3361 gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc
     3421 tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca
     3481 tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga
     3541 ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca
     3601 agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga
     3661 ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag
     3721 tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc
     3781 cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg
     3841 tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg
     3901 tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc
     3961 ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga
     4021 tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg
     4081 atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc
     4141 accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga
     4201 ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg
     4261 aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg
     4321 ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact
     4381 tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaaCTCGAG GAATTcCGAC
     4441 TCAATTAGTT CAGTCAGTTT CAGGATATTA GTCATCTCTA CATTGATTAT GAGTATTCAG
     4501 AAATTCCTTA AATATTCTGA CAAATGCTCT TTCCCTAAAC TCCCCCCATA AAAAAACCCG
     4561 CCGAAGCGGG TTTTTACGTT ATTTGCGGAT TAACGATTAC TCGTTATCAG AACCGCCCAG
     4621 ACCTGCGTTC AGCAGTTCTG CCAGGCTGGC AGATGCGTCT TCCgGAATTG ATCCGTCGAC
     4681 CAAAGCCCGC CGAAAGGCGG GCTTTTCTGT GCCGGCATat ggcggcccca cgggtgcgca
     4741 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     4801 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     4861 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     4921 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     4981 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     5041 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     5101 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     5161 ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc
     5221 ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc
     5281 tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt
     5341 accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     5401 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     5461 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     5521 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     5581 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     5641 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5701 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5761 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5821 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5881 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5941 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6001 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6061 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6121 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6181 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6241 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttac
//