LOCUS       XLoneCas9GFP           10527 bp ds-DNA     circular     06-MAR-2021
DEFINITION  .
ACCESSION   pVec1                                                              
VERSION     pVec1.1                                                            
FEATURES             Location/Qualifiers
     promoter        9..376
                     /label="TRE3GS promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    17..35
                     /label="tet operator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    53..71
                     /label="tet operator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    89..107
                     /label="tet operator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    125..143
                     /label="tet operator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    161..179
                     /label="tet operator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    197..215
                     /label="tet operator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    233..251
                     /label="tet operator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    383..4486
                     /label="Cas9"
                     /ApEinfo_revcolor=#bc8dbf
                     /ApEinfo_fwdcolor=#bc8dbf
     misc_feature    4498..4519
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4535..4588
                     /label="T2A"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4589..5308
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     source          join(5310..10527,1..377)
                     /label="synthetic DNA construct"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     polyA_signal    5489..5623
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     repeat_region   5899..5933
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(5944..5961)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             6032..6100
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        6434..6538
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             6539..7399
                     /label="AmpR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             6737..7396
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      7494..8176
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      7570..8158
                     /label="ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    8446..8467
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        8482..8512
                     /label="lac promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_binding    8516..8538
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    8520..8536
                     /label="lac operator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     8544..8564
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_region   8586..8648
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     polyA_signal    8824..8905
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(9022..9420)
                     /label="BSD"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(9421..9477)
                     /label="P2A"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(9487..10230)
                     /label="Tet-On(R) 3G"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(10231..10254)
                     /label="FLAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    10254..10263
                     /label="vertebrate consensus sequence for stronginitiationof translation (Kozak, 1987)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(10283..10494)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccatggacaa gaagtactcc attgggctcg atatcggcac
      421 aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa
      481 agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt
      541 cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac
      601 ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt
      661 ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca
      721 cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc
      781 aaccatatat catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt
      841 gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga
      901 cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa
      961 tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag
     1021 cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa
     1081 gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa
     1141 atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga
     1201 tgatctcgac aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc
     1261 aaagaacctg tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac
     1321 caaagctccg ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac
     1381 tttgctgaag gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga
     1441 tcagtctaaa aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta
     1501 caaatttatt aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct
     1561 taacagagaa gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca
     1621 gattcacctg ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt
     1681 gaaagataac agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg
     1741 ccccctcgcc cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat
     1801 cactccctgg aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga
     1861 aaggatgact aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct
     1921 gctgtacgag tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg
     1981 gatgagaaag ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt
     2041 caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga
     2101 atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac
     2161 gtatcacgat ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga
     2221 ggacattctt gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga
     2281 agaacgcttg aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag
     2341 gcgccgatat acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa
     2401 gcagagtgga aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt
     2461 catgcagttg atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt
     2521 ttctggccag ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat
     2581 caaaaaggga atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag
     2641 gcataagccc gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg
     2701 acagaagaac agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc
     2761 ccaaatcctt aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct
     2821 gtactacctg cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct
     2881 ctccgactac gacgtggatc atatcgtgcc ccagtctttt ctcaaagatg attctattga
     2941 taataaagtg ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga
     3001 agaagttgtc aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac
     3061 acaacggaag ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa
     3121 agccggcttc atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca
     3181 aattctcgat tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt
     3241 gaaagttatt actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta
     3301 taaggtgaga gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt
     3361 aggcactgca cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta
     3421 taaagtgtac gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac
     3481 cgctaagtac ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc
     3541 caatggagag attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt
     3601 gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa
     3661 catcgttaaa aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa
     3721 aaggaacagc gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg
     3781 attcgattct cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa
     3841 gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag
     3901 cttcgaaaaa aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga
     3961 cctcatcatt aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat
     4021 gctcgctagt gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt
     4081 taatttcttg tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga
     4141 gcagaagcag ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat
     4201 aagcgaattc tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc
     4261 ttacaataag cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt
     4321 tactctgacc aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag
     4381 aaagcggtac acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac
     4441 ggggctctat gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgaccc
     4501 caagaagaag aggaaggtga ggtccggcgg cggagagggc agaggaagtc ttctaacatg
     4561 cggtgacgtg gaggagaatc ccggcccaat ggtgagcaag ggcgaggagc tgttcaccgg
     4621 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4681 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4741 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4801 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4861 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4921 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4981 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5041 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5101 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5161 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5221 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5281 tctcggcatg gacgagctgt acaagtaaac tagtagacca cctcccctgc gagctaagct
     5341 ggacagccaa tgacgggtaa gagagtgaca tttttcacta acctaagaca ggagggccgt
     5401 cagagctact gcctaatcca aagacgggta aaagtgataa aaatgtatca ctccaaccta
     5461 agacaggcgc agcttccgag ggatttgaga tccagacatg ataagataca ttgatgagtt
     5521 tggacaaacc aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc
     5581 tattgcctta tttgtaacca ttataagctg caataaacaa gtttgatatc tataacaaga
     5641 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     5701 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     5761 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     5821 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     5881 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaagaa
     5941 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6001 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6061 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     6121 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     6181 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     6241 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     6301 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     6361 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6421 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6481 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6541 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6601 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6661 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6721 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6781 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6841 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6901 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6961 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7021 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7081 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7141 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7201 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7261 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7321 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7381 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7441 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7501 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7561 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7621 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7681 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7741 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7801 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7921 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7981 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8041 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8101 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8161 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8221 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8281 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8341 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8401 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8461 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     8521 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaaggtcga
     8581 cttaacccta gaaagataat catattgtga cgtacgttaa agataatcat gcgtaaaatt
     8641 gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga atagatatta
     8701 agttttatta tatttacact tacatactaa taataaattc aacaaacaat ttatttatgt
     8761 ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
     8821 ttagcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     8881 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     8941 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     9001 tgattatgat cctctggaga tttagccctc ccacacataa ccagagggca gcaattcacg
     9061 aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga tcccaggatg
     9121 cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt
     9181 tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag tgcccagcac
     9241 cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag tgaagatgcg
     9301 gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg ggatgctgtt
     9361 gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag gcttggccat
     9421 ggggccgggg ttctcctcca cgtcgccggc ctgcttcagc aggctgaagt tggtggcgcc
     9481 gctgcccccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc
     9541 aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat cgtcaagggc
     9601 gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata tgattagttc
     9661 caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa ttgcttgttt
     9721 cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg ctacttgatg
     9781 ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca gagcgtacag
     9841 tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt ccagggtttc
     9901 gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc gatgtgagag
     9961 gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg actcgccttc
    10021 cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg catcgagcag
    10081 ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca gcttttgagc
    10141 gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca gagcagagtt
    10201 tatgactttg ctcttgtcca gtctagacat cttatcgtca tcgtctttgt aatccatggt
    10261 ggcggatccc gcgtcacgac acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt
    10321 tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc
    10381 agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca ccggttcaat
    10441 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
    10501 gggcaccgga gccactcgag tggaatt
//