LOCUS       Exported                7239 bp ds-DNA     circular SYN 04-MAY-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported May 4, 2021 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    378..509
                     /label=5'ITR
                     /note="color: #00ffff"
     misc_feature    570..1041
                     /label=hSyn
                     /note="color: #a3ff98"
     misc_feature    1059..1064
                     /label=BamHI
                     /note="color: #f5ff7b"
     misc_feature    1083..1115
                     /label=lox2722
                     /note="color: #faffaf"
     misc_feature    1167..1199
                     /label=loxP
                     /note="color: #ffcd9e"
     CDS             complement(1209..1922)
                     /codon_start=1
                     /label=mRuby3
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    complement(1932..2309)
                     /label=H2B
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(2316..2372)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2388..2582)
                     /codon_start=1
                     /label=ST
                     /note="color: #339966"
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             complement(2583..2606)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    2607..2621
                     /label=Linker
                     /note="This feature has 2 segments:
                        1: 2607 .. 2619 / #a6acb3
                        2: 2620 .. 2621 / #a6acb3"
     CDS             complement(2622..3596)
                     /codon_start=1
                     /label=Chronos
                     /note="color: #a6acb3"
                     /translation="METAATMTHAFISAVPSAEATIRGLLSAAAVVTPAADAHGETSNA
                     TTAGADHGCFPHINHGTELQHKIAVGLQWFTVIVAIVQLIFYGWHSFKATTGWEEVYVC
                     VIELVKCFIELFHEVDSPATVYQTNGGAVIWLRYSEWLLTCPVILIHLSNLTGLHEEYS
                     KRTMTILVTDIGNIVWGITAAFTKGPLKILFFMIGLFYGVTCFFQIAKVYIESYHTLPK
                     GVCRKICKIMAYVFFCSWLMFPVMFIAGHEGLGLITPYTSGIGHLILDLISKNTWGFLG
                     HHLRVKIHEHILIHGDIRKTTTINVAGENMEIETFVDEEEEGGV"
     misc_feature    3607..3638
                     /label=lox2722(1)
                     /note="color: #fffab7"
     misc_feature    3690..3722
                     /label=loxP(1)
                     /note="color: #ff604e"
     misc_feature    3736..3741
                     /label=HindIII
                     /note="color: #fffba2"
     misc_feature    3742..4346
                     /label=WPRE
                     /note="color: #47ff46"
     primer_bind     complement(3801..3821)
                     /label=WPRE-R
                     /note="color: black; sequence: catagcgtaaaaggagcaaca"
     primer_bind     3801..3821
                     /label=WPRE-F
                     /note="color: black; sequence: tgttgctccttttacgctatg"
     misc_feature    4385..4597
                     /label=SV40 polyA
                     /note="color: #996633"
     misc_feature    4668..4798
                     /label=3' ITR
                     /note="color: #00ffff"
     misc_feature    4980..5297
                     /label=f1 origin
                     /note="color: #ff8000"
     misc_feature    5561..6420
                     /label=Amp
                     /note="color: #00ffff"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcaagct gatagcaagg atcctctaga gtcgactccg
     1081 gaataacttc gtataggata ctttatacga agttatgcag aatggtagct ggattgtagc
     1141 tgctattagc aatatgaaac ctcttaataa cttcgtatag catacattat acgaagttat
     1201 ggcgcgcctc acttgtacag ctcgtccatg ccaccaccaa ggttgctgta tttggcaact
     1261 gccacttctc tttgcactac gtaggtttca ttgtcactct cctcgatcct ttccaggcgg
     1321 tgatcaacgg catggacacc gggcatcttg atgttcccga cggtcttttt tgacctgtaa
     1381 gttgtcacga agttgcagtg cagatggcca ccaccatcaa ctttcagtgc gatgtcagtg
     1441 tatcctctca gaccaccatc tgctggatac atcatctctg tattaggctc ccaacccttg
     1501 gtcttcttct gcatcacggg accattggag ggaaagttta cccctctgac cttgacgttg
     1561 tagacgagct cgccatcctc aaggctggtg tcctgcgtga cggtgacgac tccaccatct
     1621 tcgtatctcg taactctttc ccaagtaaaa ccctcaggaa aggactgttt aaagaaatca
     1681 gggatgtcgg ccgggtactt gataaaggta cggctgccat acatgaacga cgtggcaaga
     1741 atgtcaaagg caaatggcag gggtcctccc tcgatgactt tgatcctcat ggtttgcact
     1801 ccctcgtacg gtctgccttc tccttcacct gtgcatttga attggtggcc gttgaccgaa
     1861 ccttccatga ccaccttcat acgcatattt tccttgatca gctcttcgcc cttagacacc
     1921 atctcgagtg ccttagcgct ggtgtacttg gtgatggcct tagtaccctc ggacacggcg
     1981 tgcttggcca actccccagg cagcagcagg cgcacggccg tctggatctc cctggaggtg
     2041 atggtcgagc gcttgttgta atgcgccagg cgggaagcct cacctgcgat gcgctcgaaa
     2101 atgtcgttca caaacgaatt catgatgccc atggccttgg acgaaatgcc ggtgtcaggg
     2161 tggacctgct tcagaacctt gtacacatag atggaatagc tctccttgcg gctgcgcttg
     2221 cgcttcttgc cgcctttctt ctgcgcctta gtcaccgcct tcttggagcc ctttttcggg
     2281 gcgggagcag acttcgctgg ctctggcata ctagtaggtc cagggttctc ctccacgtct
     2341 ccagcctgct tcagcaggct gaagttagta gctccgcttc ctgccgcgaa tctggtggct
     2401 tcagggaagt ccgtggcaca gctgatgaag ctgtcaatgc tggacatgct ccgcatgtcg
     2461 atgacgccct ccgtgcgtgc gggcagaggg gccacggggc tgggcatgct gctcatctcc
     2521 agctcctctg gaggcttgct ctgcggggcc atctccttgg tgttgaggat gggctgggac
     2581 tgcttatcgt catcgtcttt gtaatctacc ggtgcggccg ccactcctcc ctcctcctcc
     2641 tcgtcgacga aggtctcgat ctccatgttc tcgccggcca cgttgatggt ggttgtcttc
     2701 cggatgtcgc cgtggatcag gatgtgctcg tggatcttca ctctcaggtg gtggcccagg
     2761 aagccccaag tgttcttgct gatcagatcc aggatcaggt ggccgattcc gctggtgtaa
     2821 ggtgtgatca ggcccagtcc ctcgtgtccg gcgatgaaca tcacggggaa catcagccaa
     2881 gagcagaaga agacgtaggc catgatcttg caaatcttcc ggcagacgcc tttgggcagg
     2941 gtgtggtagc tctcgatata caccttggcg atctggaaga agcaagtcac gccgtagaac
     3001 aggccgatca tgaagaacag gatcttcagg gggccctttg taaaggcggc tgtgatcccc
     3061 cacacgatgt tgccgatgtc ggtcaccagg atggtcatgg tccgcttgct gtactcttcg
     3121 tgcagtccgg tcaggttgct caggtggatc aggatcacgg ggcaagtcag gagccactcg
     3181 ctgtaccgca gccaaatcac ggctcctccg ttggtctggt acactgtggc tgggctgtcg
     3241 acctcgtgga acagctcgat gaagcacttg acgagctcga tcacgcagac gtagacctcc
     3301 tcccagccgg ttgtggcctt gaagctgtgc caaccgtaga agatgagctg cacgatagcc
     3361 acgatcacgg tgaaccactg gagtcccact gcgatcttgt gctgcagctc ggttccgtgg
     3421 ttgatgtggg ggaagcaacc gtgatcggct ccggctgttg tggcgttaga ggtttctccg
     3481 tgagcgtctg ctgctggtgt caccactgct gcggcgctca gcaggcctct aattgtggct
     3541 tcggcgctag gcacggctga gataaaggcg tgggtcattg tggcggctgt ttccatactg
     3601 ctagcataac ttcgtataaa gtatcctata cgaagttatt tgccttaacc cagaaattat
     3661 cactgttatt ctttagaatg gtgcaaagaa taacttcgta taatgtatgc tatacgaagt
     3721 tatgaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     3781 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3841 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3901 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3961 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4021 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4081 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4141 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4201 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4261 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4321 tgggccgcct ccccgcatcg ataccgtcga cctcgacccg ggcggccgct tcgagcagac
     4381 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4441 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4501 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     4561 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga
     4621 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
     4681 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     4741 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct
     4801 taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4861 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4921 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4981 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5041 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5101 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5161 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5221 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5281 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5341 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5401 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5461 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5521 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5581 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5641 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5701 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5761 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5821 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5881 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5941 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6001 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6061 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6121 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6181 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6241 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6301 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6361 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6421 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6481 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6541 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7201 ttacggttcc tggccttttg ctggcctttt gctcacatg
//
