LOCUS       Exported                7684 bp ds-DNA     circular SYN 04-MAY-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported May 4, 2021 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    378..509
                     /label=5'ITR
                     /note="color: #00ffff"
     promoter        823..1100
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1511..1515
                     /label=BamHI
                     /note="color: #f5ff7b"
     misc_feature    1534..1566
                     /label=lox2722
                     /note="color: #faffaf"
     misc_feature    1618..1650
                     /label=loxP
                     /note="color: #ffcd9e"
     CDS             complement(1660..2373)
                     /codon_start=1
                     /label=mRuby3
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    complement(2383..2760)
                     /label=H2B
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(2767..2823)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2839..3033)
                     /codon_start=1
                     /label=ST
                     /note="color: #339966"
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             complement(3034..3057)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    3058..3063
                     /label=Linker
                     /note="color: #a6acb3"
     misc_feature    3065..3066
                     /label=Linker
                     /note="color: #a6acb3"
     CDS             complement(3067..4041)
                     /codon_start=1
                     /label=Chronos
                     /note="color: #a6acb3"
                     /translation="METAATMTHAFISAVPSAEATIRGLLSAAAVVTPAADAHGETSNA
                     TTAGADHGCFPHINHGTELQHKIAVGLQWFTVIVAIVQLIFYGWHSFKATTGWEEVYVC
                     VIELVKCFIELFHEVDSPATVYQTNGGAVIWLRYSEWLLTCPVILIHLSNLTGLHEEYS
                     KRTMTILVTDIGNIVWGITAAFTKGPLKILFFMIGLFYGVTCFFQIAKVYIESYHTLPK
                     GVCRKICKIMAYVFFCSWLMFPVMFIAGHEGLGLITPYTSGIGHLILDLIAKNTWGFLG
                     HHLRVKIHEHILIHGDIRKTTTINVAGENMEIETFVDEEEEGGV"
     misc_feature    3796..3813
                     /label=seq primer 3
                     /note="color: #a6acb3"
     misc_feature    4052..4083
                     /label=lox2722(1)
                     /note="color: #fffab7"
     misc_feature    4135..4167
                     /label=loxP(1)
                     /note="color: #ff604e"
     misc_feature    4181..4186
                     /label=HindIII
                     /note="color: #fffba2"
     misc_feature    4187..4791
                     /label=WPRE
                     /note="color: #47ff46"
     misc_feature    4830..5042
                     /label=SV40 polyA
                     /note="color: #996633"
     misc_feature    5113..5243
                     /label=3' ITR
                     /note="color: #00ffff"
     misc_feature    5425..5742
                     /label=f1 origin
                     /note="color: #ff8000"
     misc_feature    6006..6865
                     /label=Amp
                     /note="color: #00ffff"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaagatctg taccattgac gtcaataatg acgtatgttc
      601 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      661 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      721 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      781 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
      841 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
      901 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg
      961 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
     1021 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     1081 agcgaagcgc gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc
     1141 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     1201 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
     1261 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
     1321 agcggctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     1381 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     1441 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     1501 caaagaattg gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg
     1561 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
     1621 acttcgtata gcatacatta tacgaagtta tggcgcgcct cacttgtaca gctcgtccat
     1681 gccaccacca aggttgctgt atttggcaac tgccacttct ctttgcacta cgtaggtttc
     1741 attgtcactc tcctcgatcc tttccaggcg gtgatcaacg gcatggacac cgggcatctt
     1801 gatgttcccg acggtctttt ttgacctgta agttgtcacg aagttgcagt gcagatggcc
     1861 accaccatca actttcagtg cgatgtcagt gtatcctctc agaccaccat ctgctggata
     1921 catcatctct gtattaggct cccaaccctt ggtcttcttc tgcatcacgg gaccattgga
     1981 gggaaagttt acccctctga ccttgacgtt gtagacgagc tcgccatcct caaggctggt
     2041 gtcctgcgtg acggtgacga ctccaccatc ttcgtatctc gtaactcttt cccaagtaaa
     2101 accctcagga aaggactgtt taaagaaatc agggatgtcg gccgggtact tgataaaggt
     2161 acggctgcca tacatgaacg acgtggcaag aatgtcaaag gcaaatggca ggggtcctcc
     2221 ctcgatgact ttgatcctca tggtttgcac tccctcgtac ggtctgcctt ctccttcacc
     2281 tgtgcatttg aattggtggc cgttgaccga accttccatg accaccttca tacgcatatt
     2341 ttccttgatc agctcttcgc ccttagacac catctcgagt gccttagcgc tggtgtactt
     2401 ggtgatggcc ttagtaccct cggacacggc gtgcttggcc aactccccag gcagcagcag
     2461 gcgcacggcc gtctggatct ccctggaggt gatggtcgag cgcttgttgt aatgcgccag
     2521 gcgggaagcc tcacctgcga tgcgctcgaa aatgtcgttc acaaacgaat tcatgatgcc
     2581 catggccttg gacgaaatgc cggtgtcagg gtggacctgc ttcagaacct tgtacacata
     2641 gatggaatag ctctccttgc ggctgcgctt gcgcttcttg ccgcctttct tctgcgcctt
     2701 agtcaccgcc ttcttggagc cctttttcgg ggcgggagca gacttcgctg gctctggcat
     2761 actagtaggt ccagggttct cctccacgtc tccagcctgc ttcagcaggc tgaagttagt
     2821 agctccgctt cctgccgcga atctggtggc ttcagggaag tccgtggcac agctgatgaa
     2881 gctgtcaatg ctggacatgc tccgcatgtc gatgacgccc tccgtgcgtg cgggcagagg
     2941 ggccacgggg ctgggcatgc tgctcatctc cagctcctct ggaggcttgc tctgcggggc
     3001 catctccttg gtgttgagga tgggctggga ctgcttatcg tcatcgtctt tgtaatctac
     3061 cggtgccact cctccctcct cctcctcgtc gacgaaggtc tcgatctcca tgttctcgcc
     3121 ggccacgttg atggtggttg tcttccggat gtcgccgtgg atcaggatgt gctcgtggat
     3181 cttcactctc aggtggtggc ccaggaagcc ccaagtgttc ttggcgatca gatccaggat
     3241 caggtggccg attccgctgg tgtaaggtgt gatcaggccc agtccctcgt gtccggcgat
     3301 gaacatcacg gggaacatca gccaagagca gaagaagacg taggccatga tcttgcaaat
     3361 cttccggcag acgcctttgg gcagggtgtg gtagctctcg atatacacct tggcgatctg
     3421 gaagaagcaa gtcacgccgt agaacaggcc gatcatgaag aacaggatct tcagggggcc
     3481 ctttgtaaag gcggctgtga tcccccacac gatgttgccg atgtcggtca ccaggatggt
     3541 catggtccgc ttgctgtact cttcgtgcag tccggtcagg ttgctcaggt ggatcaggat
     3601 cacggggcaa gtcaggagcc actcgctgta ccgcagccaa atcacggctc ctccgttggt
     3661 ctggtacact gtggctgggc tgtcgacctc gtggaacagc tcgatgaagc acttgacgag
     3721 ctcgatcacg cagacgtaga cctcctccca gccggttgtg gccttgaagc tgtgccaacc
     3781 gtagaagatg agctgcacga tagccacgat cacggtgaac cactggagtc ccactgcgat
     3841 cttgtgctgc agctcggttc cgtggttgat gtgggggaag caaccgtgat cggctccggc
     3901 tgttgtggcg ttagaggttt ctccgtgagc gtctgctgct ggtgtcacca ctgctgcggc
     3961 gctcagcagg cctctaattg tggcttcggc gctaggcacg gctgagataa aggcgtgggt
     4021 cattgtggcg gctgtttcca tactgctagc ataacttcgt ataaagtatc ctatacgaag
     4081 ttatttgcct taacccagaa attatcactg ttattcttta gaatggtgca aagaataact
     4141 tcgtataatg tatgctatac gaagttatga attcgatatc aagcttatcg ataatcaacc
     4201 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4261 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4321 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4381 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4441 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4501 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4561 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4621 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4681 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4741 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     4801 acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
     4861 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4921 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4981 tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     5041 taaaatcgat aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa
     5101 tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     5161 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     5221 cagtgagcga gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac
     5281 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5341 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5401 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5461 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5521 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5581 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5641 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5701 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5761 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5821 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     5881 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5941 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6001 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6061 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6121 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6181 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6241 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6301 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6361 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6421 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6481 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6541 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6601 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6661 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6721 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6841 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7681 catg
//
