LOCUS       AAV_EFS_GFP             3930 bp    DNA        circular     08-APR-2021
DEFINITION  .
ACCESSION   pVec1                                                              
VERSION     pVec1.1                                                            
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(19..38)
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#a020f0"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    520..525
                     /locus_tag="SacII"
                     /label="SacII"
                     /ApEinfo_label="SacII"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      832..1420
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     regulatory      1002..1007
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1321..1340
                     /locus_tag="pBR322ori-F"
                     /label="pBR322ori-F"
                     /ApEinfo_label="pBR322ori-F"
                     /ApEinfo_fwdcolor="#a020f0"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     regulatory      1359..1364
                     /locus_tag="Kozak(1)"
                     /label="Kozak(1)"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     repeat_region   1482..1611
                     /locus_tag="AAV2 ITR (alternate)"
                     /label="AAV2 ITR (alternate)"
                     /ApEinfo_label="AAV2 ITR (alternate)"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        1656..1867
                     /locus_tag="EF-1-alpha core promoter"
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_label="EF-1-alpha core promoter"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     regulatory      1731..1736
                     /locus_tag="Kozak(2)"
                     /label="Kozak(2)"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1871..1876
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1880..1885
                     /locus_tag="ClaI"
                     /label="ClaI"
                     /ApEinfo_label="ClaI"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1886..1891
                     /locus_tag="AgeI"
                     /label="AgeI"
                     /ApEinfo_label="AgeI"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1892..2611
                     /locus_tag="eGFP"
                     /label="eGFP"
                     /ApEinfo_label="eGFP"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     regulatory      2003..2008
                     /locus_tag="Kozak(3)"
                     /label="Kozak(3)"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    2618..2666
                     /locus_tag="synthetic polyadenylation signal"
                     /label="synthetic polyadenylation signal"
                     /ApEinfo_label="synthetic polyadenylation signal"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2666..2673
                     /locus_tag="NotI"
                     /label="NotI"
                     /ApEinfo_label="NotI"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     repeat_region   2674..2803
                     /locus_tag="AAV2 ITR"
                     /label="AAV2 ITR"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     repeat_region   2674..2814
                     /locus_tag="AAV2 ITR(1)"
                     /label="AAV2 ITR(1)"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      2889..3344
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2976..2995)
                     /locus_tag="F1ori-R"
                     /label="F1ori-R"
                     /ApEinfo_label="F1ori-R"
                     /ApEinfo_fwdcolor="#a020f0"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3186..3207
                     /locus_tag="F1ori-F"
                     /label="F1ori-F"
                     /ApEinfo_label="F1ori-F"
                     /ApEinfo_fwdcolor="#a020f0"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3361..3380)
                     /locus_tag="pRS-marker"
                     /label="pRS-marker"
                     /ApEinfo_label="pRS-marker"
                     /ApEinfo_fwdcolor="#a020f0"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3480..3502
                     /locus_tag="pGEX 3'"
                     /label="pGEX 3'"
                     /ApEinfo_label="pGEX 3'"
                     /ApEinfo_fwdcolor="#a020f0"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3540..3558)
                     /locus_tag="pBRforEco"
                     /label="pBRforEco"
                     /ApEinfo_label="pBRforEco"
                     /ApEinfo_fwdcolor="#a020f0"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     regulatory      3602..3607
                     /locus_tag="Kozak(4)"
                     /label="Kozak(4)"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        3626..3730
                     /locus_tag="bla promoter"
                     /label="bla promoter"
                     /ApEinfo_label="bla promoter"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(3731..3930,1..661)
                     /locus_tag="bla CDS"
                     /label="bla CDS"
                     /ApEinfo_label="bla CDS"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
       61 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
      481 gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact
      541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
      661 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      721 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1441 tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc
     1501 tcgctcgctc actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
     1561 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc tgcggcctct
     1621 agaaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     1681 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     1741 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     1801 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     1861 aacacagctg aagcttggta tcgataccgg tatggtgagc aagggcgagg agctgttcac
     1921 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1981 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2041 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2101 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2161 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2221 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2281 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2341 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2401 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2461 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2521 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2581 cactctcggc atggacgagc tgtacaagta agaattcaat aaaagatctt tattttcatt
     2641 agatctgtgt gttggttttt tgtgtgcggc cgcaggaacc cctagtgatg gagttggcca
     2701 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     2761 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     2821 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     2881 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2941 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     3001 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3061 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3121 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3181 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     3241 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     3301 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     3361 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3421 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3481 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3541 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3901 ttttcgcccc gaagaacgtt ttccaatgat   
//
