LOCUS       Exported               11133 bp ds-DNA     circular SYN 07-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   wallv
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 9, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2830..6600
                     /codon_start=1
                     /label=SARS-CoV-2 S(1-1213)-2P-His6
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPASVASQSIIAYTM
                     SLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQY
                     GSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
                     SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT
                     SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGK
                     IQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQ
                     IDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
                     FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFY
                     EPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDI
                     SGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPSGRLVPRGSPGSGYI
                     PEAPRDGQAYVRKDGEWVLLSTFLGHHHHHH"
     CDS             2830..6468
                     /codon_start=1
                     /label=SARS-C0V-2 S(1-1213)
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPASVASQSIIAYTM
                     SLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQY
                     GSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
                     SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT
                     SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGK
                     IQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQ
                     IDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
                     FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFY
                     EPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDI
                     SGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPSGR"
     CDS             6469..6486
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6580..6597
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(6599..6623)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6685..6703)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    6729..6953
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6999..7427
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7441..7771
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7622..7757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7838..8632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8806..8927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8976..8992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9000..9016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9024..9054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9069..9090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9378..9966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10137..10997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10998..11102)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcgtgtt ccttgtgctg ctgccactcg tgtcatcgca gtgcgtgaac
     2881 ctgaccactc gaacacagct gccaccggcc tacaccaact cattcacccg gggagtgtac
     2941 taccctgaca aagtgttcag gagctcagtg ctgcacagca cccaggacct gttcctcccg
     3001 ttcttctcga acgtcacctg gtttcacgcg atccacgtgt ccggaactaa cggaaccaag
     3061 cgcttcgaca accccgtgct gccttttaac gacggcgtct acttcgcgtc caccgagaag
     3121 tccaacatta tcagaggctg gatcttcgga actaccctgg actccaagac ccaaagcctg
     3181 ctcattgtga acaatgccac caacgttgtg atcaaagtct gcgaattcca gttttgcaac
     3241 gacccgtttc ttggcgtgta ctaccacaag aacaacaaga gctggatgga gtccgagttc
     3301 agagtctatt cgtccgccaa caactgcacc ttcgaatacg tgtcacagcc tttcctgatg
     3361 gacctggagg gaaagcaggg gaacttcaag aacctccgcg aattcgtgtt taagaacatc
     3421 gatggttact ttaagatcta ctccaaacat actcccatta atctcgtgcg ggacctaccg
     3481 cagggattca gcgccctgga gccactggtg gacctcccca tcggcatcaa catcacccgc
     3541 tttcaaaccc tgttggcgct gcatcggagc tacctgaccc ctggagattc ctcctctggt
     3601 tggactgccg gagcagctgc atactacgtg ggctatctcc agccgcggac cttcctgctt
     3661 aagtacaatg agaacggtac tatcaccgac gccgtggatt gtgctctgga ccctctgtcc
     3721 gaaaccaagt gcaccctgaa gtcctttact gtggaaaagg gaatctacca gacctccaat
     3781 ttccgggtgc agcctaccga gtccattgtc cggttcccca acatcactaa cctgtgtcct
     3841 ttcggggaag tctttaatgc cacccgcttc gcctccgtgt acgcatggaa ccgcaagcgc
     3901 atcagcaact gcgtcgccga ctacagcgtg ctgtacaact ccgcgtcctt ctcaaccttc
     3961 aagtgctacg gggtgtcccc gactaagctg aacgacctgt gcttcacgaa cgtgtacgcc
     4021 gactccttcg tgatccgggg ggacgaagtc agacagattg cacccggaca gactggaaag
     4081 atcgcagact acaattacaa gctcccggat gacttcactg gttgcgtgat cgcttggaac
     4141 tccaacaacc tcgacagcaa agtcggcggc aactacaact atctgtaccg gctgttccgg
     4201 aagtccaacc tcaagccatt cgagcgggac atctcgaccg agatctacca ggccggttcg
     4261 actccttgca acggcgtgga ggggttcaac tgctatttcc cgctgcaaag ctacggattc
     4321 cagcccacca acggagtggg ctaccaaccg taccgggtgg tcgtcctgtc cttcgaactg
     4381 ctgcacgccc ctgctaccgt ctgcggtcca aagaagtcca ccaaccttgt gaagaacaaa
     4441 tgcgtgaact tcaacttcaa cggactcact ggcactggag tgctgactga gagcaacaag
     4501 aagttcctgc cgttccaaca atttggcaga gacatcgccg acaccactga tgccgtccgc
     4561 gatcctcaga ctctcgagat tttggatatt accccctgct cctttggggg agtcagcgtg
     4621 atcaccccgg ggaccaacac cagtaaccag gtcgcggtgc tgtaccagga cgtgaactgt
     4681 accgaagtcc cggtggccat ccacgctgac caattgaccc caacttggag ggtctactca
     4741 acggggtcca acgtgttcca aacccgcgca ggatgcctga ttggagccga gcacgtgaac
     4801 aactcctacg agtgcgacat ccctatcggt gccggaattt gtgcgagcta ccagacacag
     4861 acgaactcgc ctgcttccgt ggcctcgcag tccattattg cctacactat gagcctcggt
     4921 gccgaaaact ccgtggccta ctcgaacaac agcatcgcca ttcccaccaa cttcacgatc
     4981 tcggtgacca ccgaaatact gccggtgtcc atgactaaga cctcagtgga ttgcacaatg
     5041 tacatctgtg gcgactccac cgagtgctcc aatctcctcc tgcaatacgg ctccttctgt
     5101 acccaactga accgggcctt aactggcatc gcggtggagc aggacaagaa tacccaggaa
     5161 gtgttcgccc aagtcaaaca gatctacaag actccgccca tcaaggactt cggcggcttc
     5221 aatttctcgc aaatcctccc ggacccttca aagccctcca agcggtcctt tattgaggac
     5281 cttttgttca acaaggtcac cttggccgat gccggcttca tcaagcagta cggagactgc
     5341 ctgggagata ttgccgcgcg ggatctgatc tgcgcccaga agttcaacgg tctgactgtg
     5401 ctgccccccc tcttgaccga cgaaatgatt gctcagtaca cctccgcgct gcttgctggc
     5461 accattacca gcgggtggac gttcggagca ggagccgcac tccaaatccc gttcgccatg
     5521 caaatggcgt atcggttcaa tggtatcggc gtgacccaga acgtgctgta cgagaaccag
     5581 aagctgatcg ctaaccagtt caactccgcc atcggaaaga ttcaggactc actgtcatct
     5641 actgcgtccg ctctgggaaa gctccaggac gtggtcaatc agaacgccca ggccctcaac
     5701 acgctcgtga agcagttgag ctccaacttc ggcgccatct cgagcgtgct gaacgatatc
     5761 ctgagcagac tggatcctcc cgaggccgaa gtgcaaatcg accggttgat caccggcaga
     5821 ctgcagtcgc tgcagaccta cgtaacccag cagttgatca gggctgccga gatcagggcc
     5881 tcggccaatc tggcggccac caagatgtcg gaatgcgtgc tggggcagtc taagcgcgtg
     5941 gacttctgcg gaaagggtta ccacctgatg tcctttccgc aatcggcccc tcacggagtg
     6001 gtgtttctgc atgtcaccta cgtgcccgcc caagaaaaga acttcaccac tgcaccggcc
     6061 atctgccacg acgggaaggc gcatttcccg agagaagggg tgttcgtgag caacgggacg
     6121 cactggttcg tgacccagcg gaacttctac gagcctcaga tcatcactac cgacaacacc
     6181 ttcgtgagcg ggaactgtga tgtcgtgatt ggaattgtca acaacactgt gtacgaccca
     6241 ctgcagcccg aactggatag ctttaaggag gaactggaca agtacttcaa aaaccatacc
     6301 tcacccgatg tggacctggg cgacatctcc ggaatcaacg cctccgtggt caacatccaa
     6361 aaggagattg accgcctgaa cgaagtcgcc aagaacctga acgaatcgct gattgatctc
     6421 caagaactgg gcaaatacga gcagtacatc aagtggccgt cgggcagact tgtgccgaga
     6481 ggttcgccgg gaagcggata cattcctgaa gccccaaggg acggacaggc gtacgtgcgc
     6541 aaggacggag aatgggtgct gctgtcgact ttcctgggac accatcacca ccatcactaa
     6601 tcaactttct tgtacaaagt tgtgaaattc tgcagatatc catcacactg gcggccgctc
     6661 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     6721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6841 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6901 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6961 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7141 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc
     7381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7441 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag
     7501 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7561 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7681 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7741 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7801 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     7861 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7921 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7981 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     8041 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     8101 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     8161 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     8221 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8281 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8341 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     8401 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8461 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     8521 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     8581 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     8641 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     8701 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8761 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8941 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9001 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9061 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9121 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9541 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     9601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11101 cgcacatttc cccgaaaagt gccacctgac gtc
//
