LOCUS       Exported               11220 bp ds-DNA     circular SYN 07-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   wallv
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 9, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2830..6687
                     /label=SARS-CoV-2 S2P-3C-His8-Strep2x2 B.1.351
     CDS             2830..6453
                     /codon_start=1
                     /label=SARS CoV-2 S(1-1208) B1.351
                     /translation="MFVFLVLLPLVSSQCVNFTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFANPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRGLPQGFSA
                     LEPLVDLPIGINITRFQTLHISYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGT
                     ITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFN
                     ATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVI
                     RGDEVRQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNL
                     KPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHA
                     PATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDP
                     QTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYST
                     GSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIAYTM
                     SLGVENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQY
                     GSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
                     SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT
                     SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGK
                     IQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQ
                     IDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
                     FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFY
                     EPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDI
                     SGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQGSG"
     CDS             6541..6564
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6568..6591
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6598..6621
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6661..6684
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6686..6710)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6772..6790)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    6816..7040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7086..7514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7528..7858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7709..7844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7925..8719
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8893..9014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9063..9079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9087..9103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9111..9141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9156..9177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9465..10053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10224..11084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11085..11189)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcgtgtt ccttgtcctc ctgccgctgg tgtcatcgca gtgcgtgaac
     2881 ttcaccaccc ggactcagct gcctcctgct tacactaact ccttcactcg cggggtgtac
     2941 taccctgaca aagtgttccg gtcgtccgtg ctgcatagca cccaggacct gttccttccg
     3001 ttcttctcca acgtgacctg gttccacgcc attcacgtgt cggggaccaa cggcactaag
     3061 agattcgcca accccgtctt gcccttcaat gacggagtat acttcgcctc gacggaaaag
     3121 tccaacatca ttcggggttg gatcttcggc actaccctgg acagcaagac ccagtccctg
     3181 ctcattgtca acaacgccac caacgtcgtg atcaaagtct gcgagtttca gttttgtaac
     3241 gatccgttcc tcggcgtgta ctaccacaag aacaacaaga gctggatgga gtccgaattc
     3301 cgggtctact cctccgccaa caactgtacc ttcgaatacg tgtcccaacc tttcctcatg
     3361 gacctcgaag gaaaacaggg aaacttcaag aacttgagag agtttgtgtt caagaatatc
     3421 gatggctact tcaagatcta ctcgaagcat accccgatca acctcgtgcg gggactacct
     3481 caagggttct ccgcacttga gccgctcgtg gacctcccca ttggcatcaa catcaccaga
     3541 ttccagactc tgcatatctc gtatctcaca cccggggact cctcctccgg gtggacagct
     3601 ggtgctgcgg cctactacgt gggatatctc cagccccgga ccttcttgct gaagtacaac
     3661 gagaacggca ccatcacgga tgccgtggac tgtgcactgg accccttgag tgaaaccaag
     3721 tgcacgctga agtccttcac cgtggaaaaa ggcatctacc agaccagcaa cttcagggtg
     3781 cagcctactg aatcaatcgt gcggttccct aacattacca atctgtgccc gttcggcgaa
     3841 gtgttcaacg ccactagatt cgcctcggtg tacgcctgga accgaaagcg gatcagcaac
     3901 tgcgtggccg actactcagt gctctacaac tcggccagct tctccacctt caagtgctac
     3961 ggagtgtcgc ccaccaagct gaacgatctg tgcttcacca acgtgtacgc ggattccttc
     4021 gtgatccgcg gagatgaagt ccgccaaatc gcccccggtc aaaccggaaa cattgccgac
     4081 tacaactata agctgccgga cgactttacc ggatgcgtga ttgcctggaa ttccaacaat
     4141 ttggattcaa aagtcggcgg caactacaac tacctgtacc gcctgttccg gaagtcaaac
     4201 ctgaagccgt tcgaacgcga catcagcacc gaaatctatc aagcgggaag cactccctgc
     4261 aacggggtga agggcttcaa ctgctacttc ccgctgcaaa gctacggatt ccagcctacc
     4321 tacggcgttg gataccagcc atatcgcgtg gtcgtgctct cctttgaact tctgcacgcc
     4381 cctgccactg tgtgcggccc caagaagtcc actaacctcg tcaagaataa gtgcgtgaat
     4441 ttcaacttta acggattgac cgggaccgga gtgctcaccg agtccaacaa gaaattcctg
     4501 ccgttccaac agtttggacg ggacatcgcc gatactacgg atgctgtgcg ggacccacag
     4561 actctggaga tcctggacat tactccctgt tcctttggcg gggtgtcggt catcacccct
     4621 ggtactaaca ccagcaacca ggtcgccgtg ctgtatcagg gggtgaactg caccgaagtg
     4681 cccgtggcga tccacgccga ccagctgact ccaacttggc gcgtgtactc gactggttcg
     4741 aacgtgttcc agactcgggc cggttgcctg atcggagcgg agcatgtcaa caacagctac
     4801 gagtgcgaca tccctattgg agccgggatt tgcgccagct accagaccca gaccaattcg
     4861 cctgggtcgg ccagcagcgt ggcttcccaa tcgatcatcg cctacaccat gtccctggga
     4921 gtggagaact cagtggcgta ctccaacaac tcgattgcca tccccactaa tttcacgatc
     4981 agcgtgacca ccgagatcct gcctgtgtcc atgaccaaga cctccgtgga ctgcaccatg
     5041 tacatctgcg gagactccac cgagtgctcc aacctcctgc tgcaatacgg ctcgttctgt
     5101 acccaactga accgagccct gactgggatc gccgtggagc aggacaagaa cacacaggag
     5161 gtgttcgcac aagtcaagca gatctacaag accccgccca tcaaggattt cggagggttc
     5221 aatttctccc aaattctgcc ggacccttcc aagccgtcca agaggtcatt cattgaggat
     5281 ctcctgttca acaaggtcac cctggccgat gctggtttca tcaagcagta cggagactgc
     5341 ctgggggaca tcgcagcccg cgatctgatc tgcgcccaaa agttcaacgg cctgaccgtc
     5401 ctgcctccgc ttctgactga tgaaatgatt gcccagtaca cctctgcgct gctggctggc
     5461 actatcacct ccggctggac cttcggcgct ggagcagcac tgcagattcc atttgccatg
     5521 caaatggcct accgcttcaa cggaatcgga gtgactcaga acgtcctgta cgagaaccag
     5581 aagctcatcg ccaaccagtt caactccgcc attgggaaga ttcaggattc cctgtcgagc
     5641 actgcgtcag ccctgggaaa gctgcaggac gtcgtgaacc agaacgcaca ggccctgaac
     5701 acccttgtga agcagctgtc gtcgaacttc ggggcgatct cctccgtgtt gaacgacatc
     5761 ctgtcccggc tggacccgcc tgaggcagaa gtgcagatcg accggctgat taccggacgc
     5821 ctccagtccc ttcaaactta tgtgacccag cagctgattc gcgcggcgga aattcgggct
     5881 agcgccaatc tggctgccac caagatgagc gaatgcgtgc ttggacagtc caagagagtg
     5941 gacttttgtg gaaagggcta ccacctgatg agcttccccc aatccgcccc acacggtgtc
     6001 gtgtttctgc acgtgaccta cgtgccggcg caggaaaaga acttcaccac tgcccctgcc
     6061 atctgccacg atgggaaggc gcacttcccg agggagggag tgttcgtgtc aaacggaacc
     6121 cactggttcg tgacccaaag gaacttctac gagccccaga ttatcaccac cgataacact
     6181 ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaacactgt gtacgacccg
     6241 ctgcaaccgg agctcgatag ctttaaggaa gaactcgaca agtactttaa gaatcatacg
     6301 tcccccgatg tggatctggg cgacattagc ggtatcaacg cgtctgtcgt gaacattcag
     6361 aaggagattg acaggctcaa cgaagtcgcc aaaaacctga acgagagcct gatagacttg
     6421 caggaactgg gaaaatacga gcagggctcc ggttacatcc ccgaagctcc acgggacgga
     6481 caggcctacg tgcgcaagga cggagaatgg gtgctgctga gcaccttcct cgggcggtcc
     6541 ctggaggtgc tgttccaagg cccaggccac catcatcatc accaccacca ctcggcttgg
     6601 tcacaccccc aattcgaaaa gggcggtggt tctggaggag gcggatccgg aggatccgcg
     6661 tggagtcatc cgcagtttga aaagtaatca actttcttgt acaaagttgt gaaattctgc
     6721 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccta ttctatagtg
     6781 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6841 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6901 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6961 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7021 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7081 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7141 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7201 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg
     7261 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7321 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7381 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7441 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7501 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     7561 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7681 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7741 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7861 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     7921 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     7981 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     8041 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     8101 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     8161 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     8221 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     8281 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     8341 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     8401 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     8461 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8521 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8581 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8641 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8701 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     8761 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8821 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8881 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9001 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9061 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     9661 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9901 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
