LOCUS       Exported               11133 bp ds-DNA     circular SYN 16-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   wallv
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 9, 2021 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2830..6600
                     /codon_start=1
                     /label=SARS-CoV-2-S2P(1-1213)-His6 B.1.1.7
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAISGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIF
                     GTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYHKNNKSWMESEFRVYSSANNC
                     TFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEP
                     LVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGT
                     ITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFN
                     ATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVI
                     RGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNL
                     KPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHA
                     PATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIDDTTDAVRDP
                     QTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYST
                     GSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSHGSASSVASQSIIAYTM
                     SLGAENSVAYSNNSIAIPINFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQY
                     GSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
                     SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT
                     SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGK
                     IQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILARLDPPEAEVQ
                     IDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
                     FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFY
                     EPQIITTHNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDI
                     SGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPSGRLVPRGSPGSGYI
                     PEAPRDGQAYVRKDGEWVLLSTFLGHHHHHH"
     CDS             2830..6468
                     /codon_start=1
                     /label=SARS-CoV-2-S2P(1-1213) B.1.1.7
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAISGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIF
                     GTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYHKNNKSWMESEFRVYSSANNC
                     TFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEP
                     LVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGT
                     ITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFN
                     ATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVI
                     RGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNL
                     KPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHA
                     PATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIDDTTDAVRDP
                     QTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYST
                     GSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSHGSASSVASQSIIAYTM
                     SLGAENSVAYSNNSIAIPINFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQY
                     GSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
                     SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT
                     SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGK
                     IQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILARLDPPEAEVQ
                     IDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
                     FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFY
                     EPQIITTHNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDI
                     SGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPSGR"
     CDS             6469..6486
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6580..6597
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(6599..6623)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6685..6703)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    6729..6953
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6999..7427
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7441..7771
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7622..7757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7838..8632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8806..8927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8976..8992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9000..9016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9024..9054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9069..9090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9378..9966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10137..10997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10998..11102)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcgtgtt ccttgtcctc ctgccgctgg tgtcatcgca gtgcgtgaac
     2881 ctgaccaccc ggactcagct gcctcctgct tacactaact ccttcactcg cggggtgtac
     2941 taccctgaca aagtgttccg gtcgtccgtg ctgcatagca cccaggacct gttccttccg
     3001 ttcttctcca acgtgacctg gttccacgcc atttcgggga ccaacggcac taagagattc
     3061 gacaaccccg tcttgccctt caatgacgga gtatacttcg cctcgacgga aaagtccaac
     3121 atcattcggg gttggatctt cggcactacc ctggacagca agacccagtc cctgctcatt
     3181 gtcaacaacg ccaccaacgt cgtgatcaaa gtctgcgagt ttcagttttg taacgatccg
     3241 ttcctcggcg tgtaccacaa gaacaacaag agctggatgg agtccgaatt ccgggtctac
     3301 tcctccgcca acaactgtac cttcgaatac gtgtcccaac ctttcctcat ggacctcgaa
     3361 ggaaaacagg gaaacttcaa gaacttgaga gagtttgtgt tcaagaatat cgatggctac
     3421 ttcaagatct actcgaagca taccccgatc aacctcgtgc gggacctacc tcaagggttc
     3481 tccgcacttg agccgctcgt ggacctcccc attggcatca acatcaccag attccagact
     3541 ttactggcac tgcatagatc gtatctcaca cccggggact cctcctccgg gtggacagct
     3601 ggtgctgcgg cctactacgt gggatatctc cagccccgga ccttcttgct gaagtacaac
     3661 gagaacggca ccatcacgga tgccgtggac tgtgcactgg accccttgag tgaaaccaag
     3721 tgcacgctga agtccttcac cgtggaaaaa ggcatctacc agaccagcaa cttcagggtg
     3781 cagcctactg aatcaatcgt gcggttccct aacattacca atctgtgccc gttcggcgaa
     3841 gtgttcaacg ccactagatt cgcctcggtg tacgcctgga accgaaagcg gatcagcaac
     3901 tgcgtggccg actactcagt gctctacaac tcggccagct tctccacctt caagtgctac
     3961 ggagtgtcgc ccaccaagct gaacgatctg tgcttcacca acgtgtacgc ggattccttc
     4021 gtgatccgcg gagatgaagt ccgccaaatc gcccccggtc aaaccggaaa gattgccgac
     4081 tacaactata agctgccgga cgactttacc ggatgcgtga ttgcctggaa ttccaacaat
     4141 ttggattcaa aagtcggcgg caactacaac tacctgtacc gcctgttccg gaagtcaaac
     4201 ctgaagccgt tcgaacgcga catcagcacc gaaatctatc aagcgggaag cactccctgc
     4261 aacggggtgg agggcttcaa ctgctacttc ccgctgcaaa gctacggatt ccagcctacc
     4321 tacggcgttg gataccagcc atatcgcgtg gtcgtgctct cctttgaact tctgcacgcc
     4381 cctgccactg tgtgcggccc caagaagtcc actaacctcg tcaagaataa gtgcgtgaat
     4441 ttcaacttta acggattgac cgggaccgga gtgctcaccg agtccaacaa gaaattcctg
     4501 ccgttccaac agtttggacg ggacatcgat gatactacgg atgctgtgcg ggacccacag
     4561 actctggaga tcctggacat tactccctgt tcctttggcg gggtgtcggt catcacccct
     4621 ggtactaaca ccagcaacca ggtcgccgtg ctgtatcagg gggtgaactg caccgaagtg
     4681 cccgtggcga tccacgccga ccagctgact ccaacttggc gcgtgtactc gactggttcg
     4741 aacgtgttcc agactcgggc cggttgcctg atcggagcgg agcatgtcaa caacagctac
     4801 gagtgcgaca tccctattgg agccgggatt tgcgccagct accagaccca gaccaattcg
     4861 catgggtcgg ccagcagcgt ggcttcccaa tcgatcatcg cctacaccat gtccctggga
     4921 gccgagaact cagtggcgta ctccaacaac tcgattgcca tccccatcaa tttcacgatc
     4981 agcgtgacca ccgagatcct gcctgtgtcc atgaccaaga cctccgtgga ctgcaccatg
     5041 tacatctgcg gagactccac cgagtgctcc aacctcctgc tgcaatacgg ctcgttctgt
     5101 acccaactga accgagccct gactgggatc gccgtggagc aggacaagaa cacacaggag
     5161 gtgttcgcac aagtcaagca gatctacaag accccgccca tcaaggattt cggagggttc
     5221 aatttctccc aaattctgcc ggacccttcc aagccgtcca agaggtcatt cattgaggat
     5281 ctcctgttca acaaggtcac cctggccgat gctggtttca tcaagcagta cggagactgc
     5341 ctgggggaca tcgcagcccg cgatctgatc tgcgcccaaa agttcaacgg cctgaccgtc
     5401 ctgcctccgc ttctgactga tgaaatgatt gcccagtaca cctctgcgct gctggctggc
     5461 actatcacct ccggctggac cttcggcgct ggagcagcac tgcagattcc atttgccatg
     5521 caaatggcct accgcttcaa cggaatcgga gtgactcaga acgtcctgta cgagaaccag
     5581 aagctcatcg ccaaccagtt caactccgcc attgggaaga ttcaggattc cctgtcgagc
     5641 actgcgtcag ccctgggaaa gctgcaggac gtcgtgaacc agaacgcaca ggccctgaac
     5701 acccttgtga agcagctgtc gtcgaacttc ggggcgatct cctccgtgtt gaacgacatc
     5761 ctggcccggc tggacccgcc tgaggcagaa gtgcagatcg accggctgat taccggacgc
     5821 ctccagtccc ttcaaactta tgtgacccag cagctgattc gcgcggcgga aattcgggct
     5881 agcgccaatc tggctgccac caagatgagc gaatgcgtgc ttggacagtc caagagagtg
     5941 gacttttgtg gaaagggcta ccacctgatg agcttccccc aatccgcccc acacggtgtc
     6001 gtgtttctgc acgtgaccta cgtgccggcg caggaaaaga acttcaccac tgcccctgcc
     6061 atctgccacg atgggaaggc gcacttcccg agggagggag tgttcgtgtc aaacggaacc
     6121 cactggttcg tgacccaaag gaacttctac gagccccaga ttatcaccac ccacaacact
     6181 ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaacactgt gtacgacccg
     6241 ctgcaaccgg agctcgatag ctttaaggaa gaactcgaca agtactttaa gaatcatacg
     6301 tcccccgatg tggatctggg cgacattagc ggtatcaacg cgtctgtcgt gaacattcag
     6361 aaggagattg acaggctcaa cgaagtcgcc aaaaacctga acgagagcct gatagacttg
     6421 caggaactgg gaaaatacga gcagtacatc aagtggccca gcggacgcct tgtcccgagg
     6481 ggaagccctg gctccggtta catccccgaa gctccacggg acggacaggc ctacgtgcgc
     6541 aaggacggag aatgggtgct gctgagcacc ttcctcgggc atcatcacca ccaccactaa
     6601 tcaactttct tgtacaaagt tgtgaaattc tgcagatatc catcacactg gcggccgctc
     6661 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     6721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6841 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6901 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6961 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7141 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc
     7381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7441 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag
     7501 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7561 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7681 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7741 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7801 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     7861 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7921 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7981 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     8041 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     8101 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     8161 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     8221 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8281 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8341 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     8401 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8461 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     8521 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     8581 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     8641 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     8701 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8761 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8941 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9001 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9061 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9121 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9541 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     9601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11101 cgcacatttc cccgaaaagt gccacctgac gtc
//
