LOCUS       Exported               15921 bp ds-DNA     circular SYN 09-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 9, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
COMMENT     >pT2A_mini-slc1a3b_P-myrGCaMP6s-P2A-H2AmCherry
FEATURES             Location/Qualifiers
     source          1..15921
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             323..982
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1080..1762
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     promoter        2068..2097
                     /locus_tag="lac_promoter"
                     /label=lac_promoter
     misc_binding    2102..2124
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     2130..2150
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     2168..2187
                     /locus_tag="T3"
                     /label=T3
     primer_bind     2187..2208
                     /locus_tag="Tol2_BSI"
                     /label=Tol2_BSI
     protein_bind    2272..2471
                     /locus_tag="Tol2_5'"
                     /label=Tol2_5'
     misc_binding    2272..2290
                     /locus_tag="5' TIR"
                     /label=5' TIR
     promoter        2496..12004
                     /locus_tag="slc1a3b_P(-9.5kb)"
                     /label=slc1a3b_P(-9.5kb)
     promoter        12005..12013
                     /locus_tag="slc1a3b_P(-9.5kb)(1)"
                     /label=slc1a3b_P(-9.5kb)
                     /label=slc1a3b_P(-9.5kb)(1)
     promoter        12014..12053
                     /locus_tag="slc1a3b"
                     /label=slc1a3b
     CDS             12054..12305
                     /locus_tag="myrGFP"
                     /label=myrGFP
     primer_bind     complement(12153..12172)
                     /locus_tag="myrGFP_N-gtR"
                     /label=myrGFP_N-gtR
     promoter        12306..12311
                     /locus_tag="slc1a3b_P(-9.5kb)(2)"
                     /label=slc1a3b_P(-9.5kb)
                     /label=slc1a3b_P(-9.5kb)(2)
     CDS             12312..13661
                     /codon_start=1
                     /locus_tag="ORF frame 1"
                     /label=ORF frame 1
                     /note="/ApEinfo_hidden="
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             12312..13661
                     /locus_tag="GCaMP6s"
                     /label=GCaMP6s
     misc_feature    12321..12338
                     /locus_tag="6xHIS"
                     /label=6xHIS
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     misc_feature    12341..12359
                     /locus_tag="Xpress_fwd_primer"
                     /label=Xpress_fwd_primer
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     misc_feature    12342..12374
                     /locus_tag="T7_leader"
                     /label=T7_leader
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     misc_feature    12342..12374
                     /locus_tag="T7 Tag"
                     /label=T7 Tag
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     misc_feature    12378..12401
                     /locus_tag="Xpress_EK"
                     /label=Xpress_EK
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     gene            12492..12761
                     /gene="ECFP (444 - 713)"
                     /locus_tag="ECFP (444 - 713)"
                     /label=ECFP (444 - 713)
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     gene            12492..12761
                     /gene="EGFP (444 - 713)"
                     /locus_tag="EGFP (444 - 713)"
                     /label=EGFP (444 - 713)
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     primer_bind     12698..12719
                     /locus_tag="EGFP-C"
                     /label=EGFP-C
     gene            12783..13214
                     /gene="EGFP (0 - 431)"
                     /locus_tag="EGFP (0 - 431)"
                     /label=EGFP (0 - 431)
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     primer_bind     complement(12826..12846)
                     /locus_tag="EGFP-N"
                     /label=EGFP-N
     misc_feature    12963..12992
                     /locus_tag="Y66 (EGFP)"
                     /label=Y66 (EGFP)
                     /note="/translation=MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSS
                     RRKWN 
                     KTGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD 
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK 
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV 
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL 
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKEL 
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG 
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 
                     *"
     transit_peptide 13662..13727
                     /locus_tag="P2A"
                     /label=P2A
     CDS             13728..14111
                     /locus_tag="zf H2A.F/Z"
                     /label=zf H2A.F/Z
     CDS             14118..14828
                     /locus_tag="mCherry (Svoboda)"
                     /label=mCherry (Svoboda)
     primer_bind     14319..14339
                     /locus_tag="mCherry-F"
                     /label=mCherry-F
     primer_bind     complement(14549..14566)
                     /locus_tag="mCherry-R"
                     /label=mCherry-R
     polyA_signal    14842..15033
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     primer_bind     complement(14862..14881)
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
     primer_bind     14916..14935
                     /locus_tag="SV40pa-R"
                     /label=SV40pa-R
     protein_bind    15094..15243
                     /locus_tag="Tol2_3'"
                     /label=Tol2_3'
     misc_binding    15227..15243
                     /locus_tag="3' TIR"
                     /label=3' TIR
     primer_bind     complement(15273..15293)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(15302..15319)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             15390..15458
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     rep_origin      15477..15783
                     /locus_tag="F1 ori"
                     /label=F1 ori
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tgggcccggc tcgaaattcg cccttcacaa agcggcatcc ctgcggacct
     2521 gctgaatgag cagagcctct gctaatggcc tgaatggttg ctgggtgatg atgagccatt
     2581 gatgcaggca gacacactga gtttgctcat cacagcgggg ctctgaaaga ccctaccgca
     2641 gatcagcctc ttatagttca aacataccaa atccaaaagg gctgttttaa aatatgttca
     2701 aatgtaaacc aaaaaaggaa acatttaggt ttttctgaga caaggtttac aaggacatca
     2761 ataatcaaat tatttgccat aatctgaata agacaagagc gctagatgtc catcgtggag
     2821 atgctgcagg tgggagaggt caccggccgg tgtacaggct gtcccttcag gatcaatgcg
     2881 aggactcgtc ggtcactggg gtcttacaag gatcagtctc atgctctcca ctcctccata
     2941 accaacacag cagctgctca ggatacgtcc tggtccaaga ttatgaaaac ctcaggaata
     3001 ataaaaaaag actcgcataa gcgtcaaatt gtaatgtcta tggggaagtg ttcccagcaa
     3061 tgaagctgcc gttaaaactc atcaaacatt catttgcatt aagtgaagtt tatttataaa
     3121 ctaacttcga gaggatcacg tgcttatgat tgaacacagc tggtcatgca ttagccaatt
     3181 catgattcac caatcagatg attcctaagc cactataaat accctaagtt ctatataaca
     3241 gccatcttcg ttttgaagaa tcccccctac tcctcctcct ttcctagatg ggtggcaagg
     3301 tggcccagtg gttagcaccg ttgcctcaca gcaagaatat cgctggtttt agtccttacc
     3361 aagtcagccg acatttctgt gcatagttta cacattcttc cagtgttcat gtgggtttcc
     3421 cccgggtcgc ccggcttcct cccaccaccc aaaaacttgc aacctacttt aattaataga
     3481 ctaatccaaa tcagcaccat agacatgcac ctagtcagta gttctctctt aagagcaatt
     3541 actatctgtt tattagctac tacagcaggg gagtttcaag atctacctga gctcaaactc
     3601 ctctctcgcc ctgcaacggg agggagcctc gggctcgagg atcttctgag ctcagggctc
     3661 tctcccggga cagcatgcca aacaaacttt ataatcaatc atcagctaag tgtgaactct
     3721 tgaaagaaaa attaacacat taaccttttc agattaaagt aagaattatt agccctcctg
     3781 tggattatta taagtttttt tttaactatt tcctaaaatg atgtttaaca gagcatggaa
     3841 tttttcacag tatttcagat aatatgtttt cttctggaga aagttttatt tgttttattt
     3901 tggctagaat aaaacttaaa atttttaaaa gccattttaa ggtcaatatt attagcccct
     3961 ttcagcaata tttttgtccg attgtctaca caccatcgtt attcacccta ttcttcgccg
     4021 acgagcgggc gcagccatct gaatcttttt gactcaagac ttccggtctc attcacttcc
     4081 attcgtttct agacattaaa aacggcttgt ttcgctgttt gatgttgcaa actgatatta
     4141 tcttatatta ttatactcgg tctatattgt catgcaaaca tttgtttgta gagcaagtag
     4201 ttcaatcgtt ttctgccgtt tattgttcct agttatttct cccataggcg acggaatcgg
     4261 aagttctgaa acaatcacga aaacaggcgc acttctgcat tttagaaaaa agtcaataca
     4321 atgagttgcc taattacctt aacttgccta attaatctaa gcctttaaat gtcactttaa
     4381 ggtcaatact agtgtcttta aaaaatctag taaaatatga tgtactgtca tcatggcaaa
     4441 gataaaataa atcagttatc agagatgtct ttttccatta aaaaacagat attggggaaa
     4501 aatatataca ggggggctaa tgattctgac atgaactaca cacacatgca tatattatat
     4561 gtgtttgtgt gtgttgtttt acttccgttt gggtctacgt caagaggaaa tgtgtgtgtg
     4621 cgagttctga tgattcattt gtactgcata agcaatagtc ttaggcgcag tgactctatt
     4681 ctcctcgatc taaaccctca cactcaacaa gttctgggaa accagtgctc tcacacgacg
     4741 gccctgctct cccgtaaaat acagcaatct tcaaatgctc tccctcaaaa ctgtaagcag
     4801 aaaggctgaa agcttctttt gtgcttcatc tcttttgtct gccgagaaga gagagagaga
     4861 ttggggaaga ggggggttcc tgctctggaa gtgtttggtc caaattacac attcaaggca
     4921 agcactgttt atgattactc tcacagagga cagacaaaag cagagacatt ccaagagccc
     4981 agtgaagagg accagctcat tctgagaaag gacagagacc tacatcataa cctcattcac
     5041 atggactcct ctaaactcag agctggggaa atgcatgtaa aactggcatg tgtgcctctg
     5101 gatcagcctg gagtcaactg gaaatggcgt tataagttgg gcatttccag tcctgaatgc
     5161 tgattggtca attcgacatt tcagacctgc taaatagaca gttgcaatca ccatgacata
     5221 ttcttccaaa tagaagtgtt tttcaataaa tagttaatat atcagatttc acttagaatg
     5281 ttttagttaa atcacaaatg ataataactc accatttact caccctcagg ttatcaaaat
     5341 tgtgtatgag cttcctctga tgaacacatg atacacttac cggccacttt attaggtacc
     5401 aactgcacaa aagagaaaac atccagtgag gagcagttct gtgggcacaa atgctttgtt
     5461 aacgccagag gtcagaggag aatggccaga ctggtcgagc taatagaaag gtttcaacct
     5521 tgtttcaacc gtggtatgca gaagagcatc tctgaacatc aaacattgag ggggacagat
     5581 tacagcagca gaagacagac actgggtgtc aatcctgtca gctaaaaaca ggaaactaaa
     5641 gctacaatcc gcacaggctc accaaaattt cacaatagaa gattgggaaa acgttgcctg
     5701 gtctgatgag tctcgattta tgctacgaca ttcggatggt aggttcagaa tttggcatca
     5761 acaacataaa agcatggatc catcctgtct tgtatcaacg gttcaggctg ctggtggtgt
     5821 attggtgtgg gggatgtttt cttggcacac tttgggccca ttagtatcaa ttgagcatca
     5881 agtcaatgcc acagcccacc tgagtcttgt tgctgaccat gtccatccct ttatgaccac
     5941 agtgtattta tcttctgatg gctacttcca gcaggataac acaccatgtc ataaagcacg
     6001 aatcatctca gcctggtttc ttgaacatga caatgagttt actgtactca aatggcctcc
     6061 acagtcacca gatctcaatc caatagagca cctttgggat gtggtgaaac gggagattcg
     6121 catcatggat gtgcagccga caaatctgca gcaactgcgt catgtcaata tagaccaaaa
     6181 tctctgagga ctatttccag tacatttttg aatctgtgcc acaaaggatt taggcagttc
     6241 tagacaaaag taggtccaac acagtactag tgatgtgtac ctaataaagt ggccggtgag
     6301 tgtaatttga taattgttgc tttgaaaaag aagtgactgg actagggggg aaatactaat
     6361 gttaggatgt ttggttgcct attttcatca aaatatcatc cttctcatca tccttttaac
     6421 atataaagtt tttatttaat acatgcttca gtcaatgagt gcgtaaaata agttgaaact
     6481 gttttaaagt taatagtgag aggaaatttt gtgaagcagc atatttcatg ttctggagtg
     6541 caaaaccact gggaaacgcc catacacact catttacaca tacactacgg acaatttagc
     6601 ttacccaatt cacctatacc gtatgtcttt ggactgtggg ggaaaccgaa gcacccagag
     6661 ggaacaggga gaacatgcag actctacaca gaaatgccaa ctgatccagc caaggcttga
     6721 accagcaacc ttcttgctgt tagacgatcg tgctacccac tgcgtcaccg tgccggccca
     6781 aaatacaaaa tataaaatag ctaataatgt tcaggttctg gcacagaatg atcactttac
     6841 atcatgggct ttcagtgtac cttcaggagc caagggttat gctttgaata tgcctgtatg
     6901 ttttttgcac cctaaaatag atggaaacac cattttttgc ccaaaatcat cttacatttt
     6961 atattaaata aataaaaata aaaaaacaca gacatcttgg ttaacctgag gttgagtaaa
     7021 taaatagatc actttcattt ttgggttatc tatcctttat ttatcctttt gtcttaaatg
     7081 tttttgtttt gttatttctg tatttgttgc attgcgagtt ccaaaaaaaa tctaaaaatg
     7141 tccaaaatag tcagtcactt gttgtctctt attgcttaat ttgatcaaag cacaaaggat
     7201 agacccttta cttggtctaa aagggtaaaa atgtaaattc aagagtgacg tcatggctca
     7261 ggaacagttc acacacacat acacacacag tgaatgaggg aaataagtag tgtttgtccg
     7321 gcctgtgaga gaggtgagtg tgtgaaacag ctctcacccc acaggacgtc ccatcacgag
     7381 agtcatctga atggcttaaa gacctgccga gagtgttaga ccctggaggg tggcgataca
     7441 ctgcgcctta acatgattta acgtactcgt tctcagccaa tgaaatccac ttgcaggaat
     7501 aaatgtgaaa catgacggga gttttaatgg tttagcgtaa agatgtaatg gtctgaaaat
     7561 ttcatatcat gatcatagtg accaaaatga tcacagtcat caatattatc acagtttggt
     7621 gaaaatgatc tgtgctatat atgtcacaca catttattat ttcacataca ttggttttac
     7681 gtgtgtgtct atgtgtcttt tgagggggaa accggagagc ccagaggaaa cccacgccaa
     7741 ctcaggggga acatgcaaac tccacacaga aacaccaact ggcccagccg ggactcgaac
     7801 cagagacctt tttgctgtga ggtgctagtg ctaaccactg agccaccatg tctccctatt
     7861 tgattttctt ccttatggta ttatttgtta aaccttcact ttttcaaact gcttcacaag
     7921 gatccatgtt cctgattcca cagcctgtca ttttggcagt tttccttcta taagctagct
     7981 ttgagttctg aaactgaaat acattgtgct tcacgcaggt gagtgggctt aacaaaccac
     8041 ttgtaggaga cactcttttc ctcttcatat gtttcattag atgtcacaaa tataataaat
     8101 atgtcacatt taggactcct acatttttcc tcagtgtgtg tgtgtgtgtg tgtgtgtgtg
     8161 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     8221 tgtgtgtgtg tgtgtgagtg aatgtatttt aaaaaaaaac tgctaaacat acttggactt
     8281 acttatcatg gcatcagtgt tatcctggtc tctagtgatt tgaagtgctt ctattatcct
     8341 cattttaagt tgctttggat atagtgtctt ttaaataaat aaatgtaaac tatcaaagca
     8401 gcacaaatgt aaatcttatc catggaaaat atgcaactta cataagtgac atacaaggta
     8461 atctttggat ttgacatata taaatgtcat ttatgcagcg tatatttcaa aacatgaccg
     8521 cttttagata tttttcatgt atcaatttgt tcaaatatat attatatata cactaccaga
     8581 taaaagtctt gtcgtctata caagttttag gaataacaaa taataacttg acttctattg
     8641 attatttggt gtcagaagta gacaattatt ttatacactt attttaccaa aataaaatgt
     8701 gattatgtct tgattttcaa ttatttaata agggcagaaa ggtctgactt agcttagaca
     8761 aaaatcttgt cacttaacag aaataatgtc cagtatagaa tataaagtca tgctgcagtg
     8821 gaaacagaat gaatattgtg tctgactccc atgagcttgg aggactgcat ccatacatct
     8881 ctgctactca aatcacttat taataaagtc atctgaaata gcaaagaaag cgttcctgca
     8941 ggactcccag agttcatcaa gattctttgg attcttcttc aatgtcttct cctacatctt
     9001 acctcagaca tgctcaatta tctttataac taatgactaa tgaatcctgg agccccttga
     9061 ccttctttgc tttcaggatc tttgatgtgg aggctgaagt atgagaagga gcgctatcct
     9121 gctgaagagt ttgccctctc ctgtggtttg taatgtaatg ggcagcacaa atgtcttgat
     9181 acctcaggct gttgatgttg ccatccactc tgcagatctc tcacacgccc ccatactaaa
     9241 tgtaacccta aaccatgatt tttccttcac caaacttgac tgatttctct gagaatcttg
     9301 ggtccttgtt ggttccaata ggtcttctgc agtatttgtg atgatttggg atgcagttca
     9361 acatgatttg tcagaaaaat ctaccttcta ccacttttcc aactgatcaa ctaaaagtca
     9421 agttattatt tgtttccctt acaactggga tcggcgacaa gactttcgtc aggtagtatt
     9481 aagttgaagt cagaattatt agccccattg tatattttcc tcatttttgt ttaacataga
     9541 tacaaatttt taaccgattc taagcataac agttttaata actgatttct aataactgat
     9601 ttctcttatc tttgccatga tgacagtaaa taatatttta ctacacattt ttcaagattc
     9661 tatattcagc ttaaagtgca tattaagggc ctaactaggt taattaggca agttccagat
     9721 atttttgcaa tatataaata aatagattct gattggaaaa ataaaattgc tctgaaagtt
     9781 agtctaacaa ccctgttctt ttgtgcagtt attatatatt ttttaaactg gacctactac
     9841 taacatgcct gtttacagtt tttctcagtc actttggtgc gtttctcaca acactattta
     9901 catttgcaca acagttaatg catttctcaa aacaattagt acaaactgca aaacctagct
     9961 gataacctgc aaaagcgtgt cacttgctca aaatggatag ttcattcctc aaaagcaagt
    10021 attgatgtca atgaaagtgt cagtttcatc aagatgaaga gtgttataaa caatgtttat
    10081 aaacaagata gtcaaatggc tttgtcatgt tttcattatg acagtttact ctgtacattt
    10141 tttcaatgca ataaagtcag cttttggtga cacttcctga aaatgctcaa gacagcacta
    10201 tatgctattt gcactgaaaa ttgaaaacta cagtaaagtt agacatcact gcatttagtg
    10261 agatgtctga gtacaagaca ctgaatatgt atgtttcaca tgtttactgc atttgcattt
    10321 gcatttctaa tttattcaca gcattgtgca gaacagtaaa tacaccctta tttacaacaa
    10381 acataaactc cctttgggta gagctgtgca caactgtaga caatattgca gaacatattg
    10441 gaactgaacc tacaacacac aggtctgtaa atcattcata taattgttca attttaaaga
    10501 cattttcagg actaaaattt aaaatctttt ataaatttgc tagacagatt ttgacaacta
    10561 gttcaacatt tttgtatgta atgactcaag taatgaaatg aggaatatta gttttatatg
    10621 gaatgactat tcagcattta caagtttagt ttaatataac tgacatgaac tgagcaaatg
    10681 ataatggtat taacagcaga gagttgtatg aaagcaattg atgcatgtcc aaaagcattt
    10741 gcaatgtgtt ggaaggaatg agaaactgat gtgcacaaat gactgattgt tttgagaaat
    10801 gcattaactc ttgtacaagt ataaatagtg ttgtgagaaa tgcaccaaag ccactgagaa
    10861 aaactataat atagttcata tgagaaacaa gccactgccc ctcctgccac tccatcatgt
    10921 gccactattg gggccccttt aaagtgtctc cagcatctcc tatttggagt cttcaaagct
    10981 cctccctgtt tgagatcctg agtttcagga gcttcttact ggtggaaggt tattaaattc
    11041 ccagcacaga ctggaaactc tgatcactca gggcatctct cagcacaaga gctcactgca
    11101 agcaggtaag cagctcttct tctctcagat atttgtggtt tatttgtttt aaccacttgc
    11161 attggaatca tttaggtaat aatttatctg aagcaaaacg tatataaata gatgaactat
    11221 cttaataatg tctttataaa taaactgatc tgaactaaat atttcatcca gaagacaatt
    11281 tattatgtag catcaaatgt gattcactct aaattgatgg atctcaacta aaagatagat
    11341 agatagatag atagatagat agatagatag atagatagat agatagatag atagatagat
    11401 agatagatag atagatagat agatagatag atagatgaat gaatggatgg atggatggat
    11461 ggatggataa agacttttct gagattttca tttactagat tgatcaagga tgggttaaac
    11521 attattattt gtttaatttt attaatgtct gataacattt cttacatttt aaattaataa
    11581 attgccattt agaaactttt tgttttgttt ttaggggttg tttaacctaa aactttattt
    11641 ttttgaagtt aaaacattgc tattgtgttg tctaaaaatg aatataaaca cagtttaata
    11701 ttgacatttt cccttaaact ctgtaagaaa tctcatctca aactgtttca tattaatgca
    11761 ctttttgtca ttttaatcaa atatatttaa atatcacaca aaccaattgt tattttcttt
    11821 tgtgctgtta cagaattaat tcaaatggct tactagtgca aactatttag tttgcattta
    11881 gatttgcatg attctctgcc gttcccgttt gatgatttgt aatgtaggtc attgtgtgga
    11941 taacggagtt aagtctcatt ctctcctctt ctctggcagt gtagggtctc ttttgggtca
    12001 ccgggtcacc ggttcagccc agccagcttg atctcagtgt gtggagcgac agcatgggca
    12061 acaaatgctg cagcaagcga caggatcagg aactggcact ggcctatccc actgggggct
    12121 acaagaaatc cgactacacc tttggccaga cgcacatcaa cagcagcggc ggcggcaaca
    12181 tgggcggcgt tcttggccag aagcataaca acggtggctc gctggactcg cgctacacgc
    12241 ccgatcccaa tcatcggggt ccgttgaaaa tcggcggaaa gggcggcgtt gacatcatca
    12301 gaccaaccgg tatgggttct catcatcatc atcatcatgg tatggctagc atgactggtg
    12361 gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatctcgcc accatggtcg
    12421 actcatcacg tcgtaagtgg aataagacag gtcacgcagt cagagctata ggtcggctga
    12481 gctcactcga gaacgtctat atcaaggccg acaagcagaa gaacggcatc aaggcgaact
    12541 tccacatccg ccacaacatc gaggacggcg gcgtgcagct cgcctaccac taccagcaga
    12601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt
    12661 ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
    12721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc ggagggagca
    12781 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
    12841 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg
    12901 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
    12961 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
    13021 agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc accatcttct
    13081 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
    13141 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
    13201 agctggagta caacctgccg gaccaactga ctgaagagca gatcgcagaa tttaaagagg
    13261 ctttctccct atttgacaag gacggggatg ggacaataac aaccaaggag ctggggacgg
    13321 tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg atcaatgaag
    13381 tagatgccga cggtgacggc acaatcgact tccctgagtt cctgacaatg atggcaagaa
    13441 aaatgaaata cagggacacg gaagaagaaa ttagagaagc gttcggtgtg tttgataagg
    13501 atggcaatgg ctacatcagt gcagcagagc ttcgccacgt gatgacaaac cttggagaga
    13561 agttaacaga tgaagaggtt gatgaaatga tcagggaagc agacatcgat ggggatggtc
    13621 aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gggaagcgga gctactaact
    13681 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg gcaggtggaa
    13741 aagcaggtaa agacagtggc aaagccaagg cgaaagcagt gtctcgctcg caaagagctg
    13801 gtcttcagtt tccagtggga cgaatccaca ggcacttgaa gacacgcact acaagccatg
    13861 gtcgagtagg agcaacagca gccgtttaca gcgcagccat tcttgaatat ctcacagctg
    13921 aagttttgga gttggcggga aatgcttcaa aagacctgaa ggtgaagcgc atcactcctc
    13981 gacatttaca gctggccatt cgaggagatg aggagctcga ttcccttatc aaggccacta
    14041 ttgctggagg aggtgtgatt ccacacatcc acaagtctct gattggtaag aagggccagc
    14101 agaaaaccgc aggggggatg gtgagcaagg gcgaggagga caacatggcc atcatcaagg
    14161 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
    14221 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
    14281 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
    14341 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
    14401 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
    14461 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
    14521 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
    14581 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
    14641 gcggccacta cgacgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
    14701 ccggcgccta caacgtcaac atcaagctgg acatcacctc ccacaacgag gactacacca
    14761 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcggcatg gacgagctgt
    14821 acaagtaaat cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca
    14881 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
    14941 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
    15001 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
    15061 gtatggctga ttatgatcct ctagatcaga tctaatactc aagtacaatt ttaatggagt
    15121 acttttttac ttttactcaa gtaagattct agccagatac ttttactttt aattgagtaa
    15181 aattttccct aagtacttgt actttcactt gagtaaaatt tttgagtact ttttacacct
    15241 ctgtcaagaa ccatatgccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
    15301 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    15361 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    15421 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
    15481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    15541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    15601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    15661 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    15721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    15781 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    15841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    15901 taacgcttac aatttaggtg g
//
