LOCUS       pG12-RLuc8\(recr        5044 bp    DNA     circular     12-APR-2021
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     misc_feature    299..310
                     /vntifkey="21"
                     /label=Long\spacer
     misc_feature    232..243
                     /vntifkey="21"
                     /label=Long\spacer
     misc_binding    349..365
                     /vntifkey="20"
                     /label=12-th
     misc_binding    330..346
                     /vntifkey="20"
                     /label=11-th
     misc_binding    311..327
                     /vntifkey="20"
                     /label=10-th
     misc_binding    282..298
                     /vntifkey="20"
                     /label=9-th
     misc_binding    263..279
                     /vntifkey="20"
                     /label=8-th
     misc_binding    244..260
                     /vntifkey="20"
                     /label=7-th
     misc_binding    215..231
                     /vntifkey="20"
                     /label=6-th
     misc_binding    196..212
                     /vntifkey="20"
                     /label=5-th
     misc_binding    177..193
                     /vntifkey="20"
                     /label=4-th
     misc_binding    148..164
                     /vntifkey="20"
                     /label=3-d
     misc_binding    129..145
                     /vntifkey="20"
                     /label=2-d
     misc_binding    110..126
                     /vntifkey="20"
                     /label=1-st
     misc_feature    165..176
                     /vntifkey="21"
                     /label=Long\spacer
     promoter        complement(4707..4735)
                     /vntifkey="30"
                     /label=Promoter_P_2
                     /note="Amp promoter"
     CDS             complement(3806..4665)
                     /vntifkey="4"
                     /label=CDS_2
                     /note="AmpR"
     rep_origin      3032..3651
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pBR322 origin"
     promoter        complement(2771..2789)
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="T7"
     polyA_signal    2520..2763
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="SV40_PA term"
     CDS             423..1358
                     /codon_start=1
                     /product="Renilla luciferase"
                     /vntifkey="4"
                     /label=hRluc
BASE COUNT     1295 a      1210 c      1233 g      1305 t         1 others
ORIGIN
        1 caccatatgg acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc 
       61 atacacatac gatttaggtg acactataga actcgagcag ctgaagcttc ggagtactgt 
      121 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagtcga ctctagcgga 
      181 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagtcgactc 
      241 tagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag 
      301 tcgactctag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc 
      361 ctccggctag tagcggagac tctagagggt atataatgga tccccgggta ccggtcgcca 
      421 ccatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt 
      481 ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg 
      541 agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctacctcc agctacctgt 
      601 ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct gatctgatcg 
      661 gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat cactacaagt 
      721 acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg 
      781 actggggggc tgctctggcc tttcactacg cctacgagca ccaagacagg atcaaggcca 
      841 tcgtccatat ggagagtgtc gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg 
      901 aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt gagaataact 
      961 tcttcgtcga gaccgtgctc ccaagcaaga tcatgcggaa actggagcct gaggagttcg 
     1021 ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc ctctcctggc 
     1081 ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt gtccgcaact 
     1141 acaacgccta ccttcgggcc agcgacgatc tgcctaagct gttcatcgag tccgaccctg 
     1201 ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc gagttcgtga 
     1261 aggtgaaggg cctccacttc ctccaggagg acgctccaga tgaaatgggt aagtacatca 
     1321 agagcttcgt ggagcgcgtg ctgaagaacg agcagtaagc ggccgcgact ctagaattcc 
     1381 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 
     1441 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 
     1501 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 
     1561 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 
     1621 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 
     1681 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 
     1741 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 
     1801 agtggcaggg cggggcgtaa ttttttaagg cagttattgg tgcccttaaa cgcctggttg 
     1861 ctacgcntga ataagtgata ataagcggat gaatggcaga aattcgccgg atctttgtga 
     1921 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct 
     1981 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt 
     2041 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta 
     2101 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg 
     2161 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc 
     2221 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct 
     2281 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat 
     2341 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta 
     2401 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta 
     2461 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag 
     2521 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 
     2581 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 
     2641 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     2701 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 
     2761 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat 
     2821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     2881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     2941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     3001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     3061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     3121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     3181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     3241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     3301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     3361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     3421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     3481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     3541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     3601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     3661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     3721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     3781 tatatgagta aacttggtct gacagttacc aatgctaatc agtgaggcac ctatctcagc 
     3841 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     3901 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     3961 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     4021 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     4081 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     4141 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     4201 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     4261 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     4321 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     4381 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     4441 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     4501 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     4561 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     4621 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     4681 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     4741 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     4801 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     4861 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
     4921 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
     4981 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 
     5041 agtg 
//
