LOCUS       pLIMESd1-4.10opt        6581 bp    DNA     circular      2-OCT-2018
DEFINITION  pEGFP-N1.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12491167|
COMMENT     VNTDBDATE|-12491167|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLIMESd1-4.10opt-T2A-mTagBFP2|
COMMENT     VNTAUTHORNAME|KaaG|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Foreign object. Author: KaaG. Original author: UNKNOWN" 
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COMMENT                          "Last Modified: 10/02/18 09:22AM" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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COMMENT                                            " N1: 1426 " 1) 
COMMENT                                      #257=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1924 " 1) 
COMMENT                                      #258=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1936 " 1) 
COMMENT                                      #259=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3312 " 1))))) "XmaI: 4 sites") 
COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #260=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #261=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #262=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #263=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602064 (4 #47# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #264=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #265=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2875 - 3057" 
COMMENT                                                    1))) "2875(d1) (61 codons)") 
COMMENT                                      #266=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602008 (4 #71# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #267=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #268=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5368 - 5814" 
COMMENT                                                    1))) "5368(d1) (149 codons)"))) 
COMMENT                                    "Phase #1 (2 frames)") 
COMMENT                              #269=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #270=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602120 (4 #46# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #271=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #272=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  161 - 3307" 
COMMENT                                                    1))) "161(d2) (1049 codons)") 
COMMENT                                      #273=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068152 (4 #62# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #274=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #275=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3908 - 4081" 
COMMENT                                                    1))) "3908(d2) (58 codons)") 
COMMENT                                      #276=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602344 (4 #63# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #277=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #278=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4553 - 5344" 
COMMENT                                                    1))) "4553(d2) (264 codons)") 
COMMENT                                      #279=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068320 (4 #76# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #280=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #281=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5609 - 5788" 
COMMENT                                                    1))) "5609(d2) (60 codons)"))) 
COMMENT                                    "Phase #2 (4 frames)") 
COMMENT                              #282=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #283=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068096 (4 #52# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #284=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #285=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2067 - 2339" 
COMMENT                                                    1))) "2067(d3) (91 codons)") 
COMMENT                                      #286=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602288 (4 #57# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #287=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #288=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3189 - 3419" 
COMMENT                                                    1))) "3189(d3) (77 codons)") 
COMMENT                                      #289=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068208 (4 #65# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #290=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #291=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4182 - 4463" 
COMMENT                                                    1))) "4182(d3) (94 codons)") 
COMMENT                                      #292=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602176 (4 #66# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #293=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #294=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4566 - 4721" 
COMMENT                                                    1))) "4566(d3) (52 codons)") 
COMMENT                                      #295=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602232 (4 #68# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #296=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #297=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4725 - 5108" 
COMMENT                                                    1))) "4725(d3) (128 codons)") 
COMMENT                                      #298=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068264 (4 #72# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #299=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #300=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5229 - 5429" 
COMMENT                                                    1))) "5229(d3) (67 codons)") 
COMMENT                                      #301=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602400 (4 #78# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #302=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #303=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  6510 - 156" 
COMMENT                                                    1))) "6510(d3) (76 codons)"))) 
COMMENT                                    "Phase #3 (7 frames)"))) "Direct strand") 
COMMENT                      #304=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #305=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 -1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #306=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068544 (4 #69# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #307=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #308=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4437 - 4628" 
COMMENT                                                    1))) "4437(c1) (64 codons)") 
COMMENT                                      #309=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068432 (4 #70# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #310=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #311=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5226 - 5417" 
COMMENT                                                    1))) "5226(c1) (64 codons)") 
COMMENT                                      #312=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602512 (4 #75# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #313=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #314=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5628 - 5789" 
COMMENT                                                    1))) "5628(c1) (54 codons)") 
COMMENT                                      #315=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068376 (4 #79# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #316=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #317=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  6039 - 6290" 
COMMENT                                                    1))) "6039(c1) (84 codons)"))) 
COMMENT                                    "Phase #1 (4 frames)") 
COMMENT                              #318=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 -2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #319=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068040 (4 #58# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #320=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #321=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  206 - 355" 1))) 
COMMENT                                            "206(c2) (50 codons)") 
COMMENT                                      #322=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068768 (4 #59# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #323=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #324=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  380 - 1648" 
COMMENT                                                    1))) "380(c2) (423 codons)") 
COMMENT                                      #325=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068712 (4 #60# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #326=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #327=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2138 - 2338" 
COMMENT                                                    1))) "2138(c2) (67 codons)") 
COMMENT                                      #328=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602680 (4 #61# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #329=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #330=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2573 - 3322" 
COMMENT                                                    1))) "2573(c2) (250 codons)") 
COMMENT                                      #331=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602624 (4 #73# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #332=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #333=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4865 - 5401" 
COMMENT                                                    1))) "4865(c2) (179 codons)") 
COMMENT                                      #334=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602456 (4 #74# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #335=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #336=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5576 - 5806" 
COMMENT                                                    1))) "5576(c2) (77 codons)"))) 
COMMENT                                    "Phase #2 (6 frames)") 
COMMENT                              #337=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 -3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #338=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84067984 (4 #48# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #339=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #340=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  505 - 714" 1))) 
COMMENT                                            "505(c3) (70 codons)") 
COMMENT                                      #341=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84067928 (4 #49# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #342=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #343=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  955 - 1281" 
COMMENT                                                    1))) "955(c3) (109 codons)") 
COMMENT                                      #344=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068824 (4 #50# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #345=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #346=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1294 - 1446" 
COMMENT                                                    1))) "1294(c3) (51 codons)") 
COMMENT                                      #347=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84067872 (4 #51# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #348=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #349=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1747 - 1965" 
COMMENT                                                    1))) "1747(c3) (73 codons)") 
COMMENT                                      #350=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84067816 (4 #53# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #351=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #352=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2182 - 2400" 
COMMENT                                                    1))) "2182(c3) (73 codons)") 
COMMENT                                      #353=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602792 (4 #54# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #354=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #355=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2458 - 2661" 
COMMENT                                                    1))) "2458(c3) (68 codons)") 
COMMENT                                      #356=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602736 (4 #55# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #357=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #358=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2800 - 2955" 
COMMENT                                                    1))) "2800(c3) (52 codons)") 
COMMENT                                      #359=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068656 (4 #56# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #360=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #361=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2959 - 3138" 
COMMENT                                                    1))) "2959(c3) (60 codons)") 
COMMENT                                      #362=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068600 (4 #64# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #363=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #364=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3814 - 4038" 
COMMENT                                                    1))) "3814(c3) (75 codons)") 
COMMENT                                      #365=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 84068488 (4 #67# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #366=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #367=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4618 - 4806" 
COMMENT                                                    1))) "4618(c3) (63 codons)") 
COMMENT                                      #368=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 82602568 (4 #77# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #369=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #370=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5533 - 5727" 
COMMENT                                                    1))) "5533(c3) (65 codons)"))) 
COMMENT                                    "Phase #3 (11 frames)"))) 
COMMENT                            "Complementary strand"))) "Open Reading Frames")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #371=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #372=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #373=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
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FEATURES             Location/Qualifiers
     promoter        33..126
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     promoter        84..153
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    3536..3555
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(3705..4011)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        4090..4118
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(4184..4204)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(4190..4405)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        4202..4470
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      4369..4446
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    4431..4450
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             4553..5347
                     /vntifkey="4"
                     /label=ORF\frame\1
     gene            4556..5344
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(4862..5398)
                     /vntifkey="4"
                     /label=ORF\frame\3
     terminator      5522..5791
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      5939..6558
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             2552..2605
                     /vntifkey="4"
                     /label=T2A
     misc_feature    167..184
                     /vntifkey="21"
                     /label=SV40\NLS
     misc_feature    188..199
                     /vntifkey="21"
                     /label=Linker
     misc_feature    200..394
                     /vntifkey="21"
                     /label=Gal4(1-65)
     misc_feature    2060..2143
                     /vntifkey="21"
                     /label=28\aa\linker\from\pmiRFP703-Clathrin\(#150083)
     misc_feature    398..445
                     /vntifkey="21"
                     /label=16\aa\linker\(SAGG)4\from\pCMV-104
     misc_feature    167..199
                     /vntifkey="21"
                     /label=NLS+linker\from\pQP-T2A\(#102583)
     misc_feature    2612..3310
                     /vntifkey="21"
                     /label=mTagBFP2
     misc_feature    2150..2533
                     /vntifkey="21"
                     /label=VP16
     misc_feature    452..2053
                     /vntifkey="21"
                     /label=4.10\codon\optimized
BASE COUNT     1576 a      1801 c      1849 g      1355 t 
ORIGIN
        1 ttgctggcct tttgctcaca tgttctttcc tgccaaaatc aacgggactt tccaaaatgt 
       61 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 
      121 ataagcagag ctggtttagt gaaccgtcag atccgctagc atggcgcgca agaagaagag 
      181 gaagtccgga ggtggtggaa agctactgtc ttctatcgaa caagcatgcg atatttgccg 
      241 acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa 
      301 ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac 
      361 agaagtggaa tcaaggctag aaagactgga acagactagt gctggtgggt ccgccggcgg 
      421 gagtgctggc ggtagtgccg gcggtgaatt cgccgccgac ctgggctctg acgatatcag 
      481 caagctgatc gccgcctgcg atcaggagcc aatccacatc cccaatgcca tccagccatt 
      541 tggcgccatg ctgatcgtgg agaaggacac acagcagatc gtgtacgcct ctgccaacag 
      601 cgccgagtac ttcagcgtgg ccgacaatac catccacgag ctgtccgatt tcaagcaggc 
      661 caacatcaat tctctgctgc ccgagcagct gatcagcggc ctgacatccg ccatctctga 
      721 gaacgagcct atctgggtgg agaccgacag gctgagcttt ctgggctggc gccacgagaa 
      781 ctactatatc atcgaggtgg agagatacca cgtgcagaca tccaattggt tcgagatcca 
      841 gtttcagcgg gccttccaga agctgagaaa ctgtaagacc cacaacgatc tgatcaatac 
      901 cctgacacgg ctgatccagg agatcagcgg ctacgacaga gtgatgatct atcagttcga 
      961 tcccgagtgg aatggcagag tgatcgccga gagcgtgaga cagctgttta cctccatgct 
     1021 gaaccaccac ttcccagcct ctgacatccc tgcacaggcc agggccatgt acagcatcaa 
     1081 cccaatccgc atcatccccg atgtgaatgc cgagccccag cctctgcaca tgatccacaa 
     1141 gccacagaac acagaggccg tgaatctgtc ctgcggcgtg ctgagggccg tgtctccact 
     1201 gcacatgcag tatctgcgca actttggcgt gtctgccagc acctccatcg gcatcttcaa 
     1261 tgaggacaag ctgtggggca tcgtggcctg tcaccacaca aagcctaggg ccatcggccg 
     1321 gagaatcagg cacctgctgg tgcgcaccgt ggagtttgca gcagagcgcc tgtggctgat 
     1381 ccactccagg aatgtggagc ggtacatggt gacagtgcag gcagcccggg agcagctgtc 
     1441 taccacagcc gacgataagc acagctccca cgagatcgtg atcgagcacg ccgccgactg 
     1501 gtgcaagctg ttccggtgtg atggcgtggg ctacctgaga ggcgaggagc tgaccacata 
     1561 tggcgagacc cctgatcaga ccacaatcaa caagctggtg gagtggctgg aggagaatgg 
     1621 caagaagagc ctgttttggc actcccacat gctgaaggag gacgcacctg gactgctgcc 
     1681 agatggcagc cggttcgcag gactgctggc catcccactg aagtctgacg ccgatctgtt 
     1741 tagctacctg ctgctgttca gggtggcaca gaacgaggtg cgcacatggg caggcaagcc 
     1801 tgagaagctg tccgtggaga cctctacagg caccatggtg ggcccacgga agtcttttga 
     1861 ggcctggcag gacgaggtga gcggcaagtc ccagccttgg agaaccgcac agctgtatgc 
     1921 agcccgggac atcgcccggg acctgctgat cgtggccgat agcatgcagc tgaacctgct 
     1981 gaatgaccag ctggccgatg ccaacgagaa tctggagaag ctggcctcct tcgacgatct 
     2041 gacctctggc ggcggtacca gcgggggtgg tggatcaggt ggaggaggtt ctggaggtgg 
     2101 tggatcagga ggaggtggtt ctggaggtgg tggatcagga ggaagatctg cgtacagccg 
     2161 cgcgcgtacg aaaaacaatt acgggtctac catcgagggc ctgctcgatc tcccggacga 
     2221 cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca 
     2281 cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg agctccactt 
     2341 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat 
     2401 gttgggggac ggggattccc ctggtccggg atttaccccc cacgactccg ccccctacgg 
     2461 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga 
     2521 cgagtacggt ggggctcgag ataggcgcgc cgagggcaga ggaagtctgc taacatgcgg 
     2581 tgacgtcgag gagaatcctg gcccaactag tagcgagctg attaaggaga acatgcacat 
     2641 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga 
     2701 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct 
     2761 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa 
     2821 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga 
     2881 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca 
     2941 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc 
     3001 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga 
     3061 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat 
     3121 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg 
     3181 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt 
     3241 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa 
     3301 gcttaattaa cccgggatcc accggggccg cgactctaga tcataatcag ccataccaca 
     3361 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 
     3421 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 
     3481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     3541 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 
     3601 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 
     3661 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 
     3721 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 
     3781 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 
     3841 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 
     3901 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 
     3961 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 
     4021 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     4081 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     4141 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 
     4201 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     4261 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 
     4321 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 
     4381 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     4441 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     4501 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 
     4561 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 
     4621 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 
     4681 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 
     4741 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 
     4801 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 
     4861 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 
     4921 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 
     4981 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 
     5041 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 
     5101 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 
     5161 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 
     5221 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 
     5281 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 
     5341 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 
     5401 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 
     5461 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 
     5521 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 
     5581 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 
     5641 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 
     5701 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 
     5761 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 
     5821 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     5881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     5941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     6001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     6061 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 
     6121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     6181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     6241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     6301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     6361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     6421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     6481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     6541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct t 
//
