LOCUS       pAAV2-G12-Cherif        5537 bp    DNA     circular SYN 13-FEB-2019
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes) .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CW3SL-EGFP.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT                           "  TITLE     Optimization of AAV expression cassettes to improve packaging " 
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COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 4888 (3 #34# 0) 1 2 2 0 194) 
COMMENT                                     (CObjectList 
COMMENT                                      #259=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 4888  End: 5476" 1) 
COMMENT                                      #260=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" 
COMMENT                                            1) 
COMMENT                                      #261=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #262=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   4888..5476" 1) 
COMMENT                                      #263=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #264=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /direction=RIGHT" 1))) 
COMMENT                                    "pBR322 origin"))) 
COMMENT                            "Replication Origin (2 total)") 
COMMENT                      #265=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 34 (7 34 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #266=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                     (CObjectList 
COMMENT                                      #267=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 1     End: 141 " 1) 
COMMENT                                      #268=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "inverted terminal repeat of adeno-associated virus serotype 2" 
COMMENT                                            1) 
COMMENT                                      #269=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #270=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   1..141" 1))) "AAV2 ITR") 
COMMENT                              #271=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 12 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                     (CObjectList 
COMMENT                                      #272=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 12    End: 141 " 1) 
COMMENT                                      #273=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #274=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   12..141" 1))) "AAV2 ITR") 
COMMENT                              #275=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2800 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                     (CObjectList 
COMMENT                                      #276=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 2800  End: 2940 (Complementary)" 
COMMENT                                            1) 
COMMENT                                      #277=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "inverted terminal repeat of adeno-associated virus serotype 2" 
COMMENT                                            1) 
COMMENT                                      #278=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #279=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   complement(2800..2940)" 1))) 
COMMENT                                    "AAV2 ITR") 
COMMENT                              #280=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2800 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                     (CObjectList 
COMMENT                                      #281=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 2800  End: 2929 (Complementary)" 
COMMENT                                            1) 
COMMENT                                      #282=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #283=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   complement(2800..2929)" 1))) 
COMMENT                                    "AAV2 ITR"))) "Repeat Region (4 total)"))) 
COMMENT                    "Feature Map") 
COMMENT              #284=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #285=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16248320 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #286=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #287=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1701 " 1) 
COMMENT                                      #288=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3476 " 1) 
COMMENT                                      #289=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3973 " 1) 
COMMENT                                      #290=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5219 " 1))))) "ApaLI: 4 sites") 
COMMENT                            1 5 "GTGCAC" "CACGTG") 
COMMENT                      #291=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16248240 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #292=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #293=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 41 " 1) 
COMMENT                                      #294=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 52 " 1) 
COMMENT                                      #295=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 218 " 1) 
COMMENT                                      #296=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 529 " 1) 
COMMENT                                      #297=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 1567 " 1) 
COMMENT                                      #298=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 1917 " 1) 
COMMENT                                      #299=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 2109 " 1) 
COMMENT                                      #300=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 8) 1 2 2 0 191) 
COMMENT                                            " N8: 2360 " 1) 
COMMENT                                      #301=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 9) 1 2 2 0 191) 
COMMENT                                            " N9: 2886 " 1) 
COMMENT                                      #302=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 10) 1 2 2 0 191) 
COMMENT                                            " N10: 2897 " 1) 
COMMENT                                      #303=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 11) 1 2 2 0 191) 
COMMENT                                            " N11: 3353 " 1))))) 
COMMENT                             "AvaI: 11 sites") 1 5 "CYCGRG" "GRGCYC") 
COMMENT                      #304=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16248160 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #305=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #306=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 524 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #307=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16248080 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                            "TAGCTA") 
COMMENT                      #308=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16248000 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #309=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #310=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2352 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #311=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16247920 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #312=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #313=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 230 " 1))))) "HindIII: 1 site") 
COMMENT                            1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #314=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16247840 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #315=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #316=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 547 " 1) 
COMMENT                                      #317=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 927 " 1) 
COMMENT                                      #318=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2074 " 1))))) "NcoI: 3 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #319=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16247600 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #320=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #321=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7 " 1) 
COMMENT                                      #322=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1996 " 1) 
COMMENT                                      #323=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2939 " 1))))) "PstI: 3 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #324=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16247520 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #325=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #326=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 43 " 1) 
COMMENT                                      #327=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 54 " 1) 
COMMENT                                      #328=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 531 " 1) 
COMMENT                                      #329=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2888 " 1) 
COMMENT                                      #330=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 2899 " 1))))) "SmaI: 5 sites") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #331=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 16260400 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #332=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #333=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 41 " 1) 
COMMENT                                      #334=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 52 " 1) 
COMMENT                                      #335=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 529 " 1) 
COMMENT                                      #336=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2886 " 1) 
COMMENT                                      #337=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 2897 " 1))))) "XmaI: 5 sites") 
COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #338=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #339=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #340=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #341=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58455944 (4 #59# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #342=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #343=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  817 - 984" 1))) 
COMMENT                                            "817(d1) (56 codons)") 
COMMENT                                      #344=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456672 (4 #73# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #345=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #346=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1930 - 2178" 
COMMENT                                                    1))) "1930(d1) (83 codons)"))) 
COMMENT                                    "Phase #1 (2 frames)") 
COMMENT                              #347=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #348=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58455832 (4 #56# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #349=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #350=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  521 - 2347" 
COMMENT                                                    1))) "521(d2) (609 codons)") 
COMMENT                                      #351=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456728 (4 #65# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #352=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #353=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3050 - 3262" 
COMMENT                                                    1))) "3050(d2) (71 codons)") 
COMMENT                                      #354=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456112 (4 #66# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #355=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #356=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3857 - 4714" 
COMMENT                                                    1))) "3857(d2) (286 codons)") 
COMMENT                                      #357=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456168 (4 #68# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #358=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #359=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5534 - 170" 
COMMENT                                                    1))) "5534(d2) (58 codons)"))) 
COMMENT                                    "Phase #2 (4 frames)") 
COMMENT                              #360=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #361=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58455888 (4 #58# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #362=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #363=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  666 - 848" 1))) 
COMMENT                                            "666(d3) (61 codons)") 
COMMENT                                      #364=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456000 (4 #61# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #365=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #366=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2457 - 2657" 
COMMENT                                                    1))) "2457(d3) (67 codons)") 
COMMENT                                      #367=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456056 (4 #69# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #368=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #369=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2775 - 3035" 
COMMENT                                                    1))) "2775(d3) (87 codons)"))) 
COMMENT                                    "Phase #3 (3 frames)"))) "Direct strand") 
COMMENT                      #370=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #371=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 -1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #372=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456336 (4 #75# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #373=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #374=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3486 - 3644" 
COMMENT                                                    1))) "3486(c1) (53 codons)"))) 
COMMENT                                    "Phase #1 (1 frame)") 
COMMENT                              #375=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 -2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #376=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456560 (4 #55# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #377=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #378=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  368 - 1399" 
COMMENT                                                    1))) "368(c2) (344 codons)") 
COMMENT                                      #379=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456896 (4 #62# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #380=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #381=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1604 - 2317" 
COMMENT                                                    1))) "1604(c2) (238 codons)") 
COMMENT                                      #382=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456448 (4 #63# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #383=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #384=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2471 - 2656" 
COMMENT                                                    1))) "2471(c2) (62 codons)") 
COMMENT                                      #385=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456392 (4 #64# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #386=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #387=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2753 - 2995" 
COMMENT                                                    1))) "2753(c2) (81 codons)") 
COMMENT                                      #388=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456952 (4 #67# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #389=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #390=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  5533 - 160" 
COMMENT                                                    1))) "5533(c2) (55 codons)"))) 
COMMENT                                    "Phase #2 (5 frames)") 
COMMENT                              #391=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 -3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #392=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456616 (4 #57# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #393=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #394=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  301 - 549" 1))) 
COMMENT                                            "301(c3) (83 codons)") 
COMMENT                                      #395=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456504 (4 #60# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #396=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #397=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1393 - 1707" 
COMMENT                                                    1))) "1393(c3) (105 codons)") 
COMMENT                                      #398=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456840 (4 #70# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #399=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #400=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2968 - 3135" 
COMMENT                                                    1))) "2968(c3) (56 codons)") 
COMMENT                                      #401=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456280 (4 #71# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #402=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #403=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4147 - 4314" 
COMMENT                                                    1))) "4147(c3) (56 codons)") 
COMMENT                                      #404=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 58456224 (4 #72# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #405=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #406=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4324 - 4587" 
COMMENT                                                    1))) "4324(c3) (88 codons)") 
COMMENT                                      #407=(CFolderPar 
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COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #408=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #409=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4972 - 5223" 
COMMENT                                                    1))) "4972(c3) (84 codons)"))) 
COMMENT                                    "Phase #3 (6 frames)"))) 
COMMENT                            "Complementary strand"))) "Open Reading Frames")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #411=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #412=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #413=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #414=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #415=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #416=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #417=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #418=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #419=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #420=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #421=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #422=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #423=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #424=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #425=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #426=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #427=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #428=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #429=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #430=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #431=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #432=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #433=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #434=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #435=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #436=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #437=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #438=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #439=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #440=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #441=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #442=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #443=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #444=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #445=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #446=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #447=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #448=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #449=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 
COMMENT         -5537) 1 1 0 1 1 
COMMENT        (mapper: 24.6574 -42.2308 40.0378 40.0378 0.01 10 -1 5537 5537 1 0 0) 
COMMENT        #450=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 14418244 100) 
COMMENT              (CObjectList 
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COMMENT                     (CObjectList 
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COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #416# 81479788 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27532 6.27332 1 0.0214037) 0.113296) 
COMMENT                      #453=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #413# 1717986918 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pAAV2-G12-Cheriff-T2A-mCherry" 
COMMENT                            "@N" 1 0 0.871165 0 -10 19.4067 1.39868 Nil) 
COMMENT                      #454=(CLabel 
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COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "5537 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.42271 0.799245 Nil)) (CObjectList)) 
COMMENT               #455=(CGroupWidget (CWidget 13 (12 0) 1 2 0 0 Nil -1846 100) 
COMMENT                     (CObjectList 
COMMENT                      #456=(CGroupWidget (CWidget 0 (13 0 0) 1 2 0 0 Nil 0 100) 
COMMENT                            (CObjectList 
COMMENT                             #457=(CGroupWidget 
COMMENT                                   (CWidget 1 (14 1 0) 1 2 0 0 Nil 1800 100) 
COMMENT                                   (CObjectList 
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COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #459=(CORFArrow 
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COMMENT                             #460=(CGroupWidget 
COMMENT                                   (CWidget 2 (14 2 0) 1 2 0 0 Nil 1955055587 
COMMENT                                    100) 
COMMENT                                   (CObjectList 
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COMMENT                                          (CLine 
COMMENT                                           (CWidget 2 (4 #56# 0) 1 2 0 0 #429# 
COMMENT                                            268632356 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.32929 3.61413 5.68405) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #462=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 2 (4 #65# 0) 1 2 0 0 #429# 
COMMENT                                            58260208 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.32929 2.57748 2.8188) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #463=(CORFArrow 
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COMMENT                                            58258368 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.32929 0.932424 1.9045) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #464=(CORFArrow 
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COMMENT                                           (CWidget 2 (4 #68# 0) 1 2 0 0 #429# 
COMMENT                                            58256024 100) (LOGPEN 0 6 16384) 8 
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COMMENT                             #465=(CGroupWidget 
COMMENT                                   (CWidget 3 (14 3 0) 1 2 0 0 Nil 0 100) 
COMMENT                                   (CObjectList 
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COMMENT                                           (CWidget 3 (4 #58# 0) 1 2 0 0 #429# 
COMMENT                                            268632316 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.49393 5.31244 5.51977) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #467=(CORFArrow 
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COMMENT                                           (CWidget 3 (4 #61# 0) 1 2 0 0 #429# 
COMMENT                                            58250800 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.49393 3.26292 3.49064) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #468=(CORFArrow 
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COMMENT                                           (CWidget 3 (4 #69# 0) 1 2 0 0 #429# 
COMMENT                                            58251040 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.49393 2.83466 3.13036) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0)) 
COMMENT                                   (CObjectList))) (CObjectList)) 
COMMENT                      #469=(CGroupWidget 
COMMENT                            (CWidget 1 (13 1 0) 1 2 0 0 Nil -2753 100) 
COMMENT                            (CObjectList 
COMMENT                             #470=(CGroupWidget 
COMMENT                                   (CWidget 1 (14 -1 0) 1 2 0 0 Nil 0 100) 
COMMENT                                   (CObjectList 
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COMMENT                                           (CWidget 1 (4 #75# 0) 1 2 0 0 #429# 0 
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COMMENT                                   (CObjectList)) 
COMMENT                             #472=(CGroupWidget 
COMMENT                                   (CWidget 2 (14 -2 0) 1 2 0 0 Nil 480 100) 
COMMENT                                   (CObjectList 
COMMENT                                    #473=(CORFArrow 
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COMMENT                                           (CWidget 2 (4 #55# 0) 1 2 0 0 #429# 
COMMENT                                            268632396 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.670712 4.68818 5.85739) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 1 0 
COMMENT                                          0) 
COMMENT                                    #474=(CORFArrow 
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COMMENT                                           (CWidget 2 (4 #62# 0) 1 2 0 0 #429# 
COMMENT                                            58262848 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.670712 3.64812 4.45705) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 1 0 
COMMENT                                          0) 
COMMENT                                    #475=(CORFArrow 
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COMMENT                                           (CWidget 2 (4 #63# 0) 1 2 0 0 #429# 
COMMENT                                            58261968 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.670712 3.26405 3.47478) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 1 0 
COMMENT                                          0) 
COMMENT                                    #476=(CORFArrow 
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COMMENT                                            58262448 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.670712 2.87998 3.15529) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 1 0 
COMMENT                                          0) 
COMMENT                                    #477=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 2 (4 #67# 0) 1 2 0 0 #429# 
COMMENT                                            58257968 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.670712 6.09191 0.00566479) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 1 0 
COMMENT                                          0)) (CObjectList)) 
COMMENT                             #478=(CGroupWidget 
COMMENT                                   (CWidget 3 (14 -3 0) 1 2 0 0 Nil 0 100) 
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COMMENT                                            268632336 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.506068 5.65119 5.9333) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 1 0 0) 
COMMENT                                    #480=(CORFArrow 
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COMMENT                                           (CWidget 3 (4 #60# 0) 1 2 0 0 #429# 
COMMENT                                            58257544 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.506068 4.33923 4.69611) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 1 0 
COMMENT                                          0) 
COMMENT                                    #481=(CORFArrow 
COMMENT                                          (CLine 
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COMMENT                                            58258928 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.506068 2.72136 2.9117) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 1 0 0) 
COMMENT                                    #482=(CORFArrow 
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COMMENT                                           0.506068 1.38561 1.57594) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 1 0 
COMMENT                                          0) 
COMMENT                                    #483=(CORFArrow 
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COMMENT                                           0.506068 1.07631 1.37541) 0.2 
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COMMENT                                          0) 
COMMENT                                    #484=(CORFArrow 
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COMMENT                                            100) (LOGPEN 0 6 16384) 8 0.506068 
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COMMENT                     (CObjectList 
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COMMENT                            (CObjectList 
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COMMENT                                    (CWidget 0 (3 #32# 0) 1 2 0 0 #414# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
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COMMENT                             #488=(CLabel (CWidget 0 (0 0) 1 2 0 0 #411# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
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COMMENT                             #489=(CWideArrow 
COMMENT                                   (CWideLine 
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COMMENT                             #490=(CLabel (CWidget 0 (0 0) 1 2 0 0 #411# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "T2A" "@N" 1 
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COMMENT                                   (LOGPEN 0 6 6723840) 8 1 5.7373 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #493=(CLabel (CWidget 0 (0 0) 1 2 0 0 #411# 0 100) 
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FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /vntifkey="34"
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus serotype 2"
     repeat_region   12..141
                     /vntifkey="34"
                     /label=AAV2\ITR
     primer_bind     complement(2420..2440)
                     /vntifkey="28"
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   complement(2800..2940)
                     /vntifkey="34"
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus serotype 2"
     repeat_region   complement(2800..2929)
                     /vntifkey="34"
                     /label=AAV2\ITR
     rep_origin      3015..3470
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     primer_bind     complement(3102..3121)
                     /vntifkey="28"
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3312..3333
                     /vntifkey="28"
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3487..3506)
                     /vntifkey="28"
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable marker"
     primer_bind     3606..3628
                     /vntifkey="28"
                     /label=pGEX\3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3666..3684)
                     /vntifkey="28"
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward primer"
     promoter        3752..3856
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     CDS             3857..4717
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     primer_bind     complement(4075..4094)
                     /vntifkey="28"
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4888..5476
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=pBR322\origin
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     5377..5396
                     /vntifkey="28"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    424..435
                     /vntifkey="21"
                     /label=Long\spacer
     misc_feature    357..368
                     /vntifkey="21"
                     /label=Long\spacer
     misc_binding    474..490
                     /vntifkey="20"
                     /label=12-th
     misc_binding    455..471
                     /vntifkey="20"
                     /label=11-th
     misc_binding    436..452
                     /vntifkey="20"
                     /label=10-th
     misc_binding    407..423
                     /vntifkey="20"
                     /label=9-th
     misc_binding    388..404
                     /vntifkey="20"
                     /label=8-th
     misc_binding    369..385
                     /vntifkey="20"
                     /label=7-th
     misc_binding    340..356
                     /vntifkey="20"
                     /label=6-th
     misc_binding    321..337
                     /vntifkey="20"
                     /label=5-th
     misc_binding    302..318
                     /vntifkey="20"
                     /label=4-th
     misc_binding    273..289
                     /vntifkey="20"
                     /label=3-d
     misc_binding    254..270
                     /vntifkey="20"
                     /label=2-d
     misc_binding    235..251
                     /vntifkey="20"
                     /label=1-st
     misc_feature    290..301
                     /vntifkey="21"
                     /label=Long\spacer
     misc_feature    548..1564
                     /vntifkey="21"
                     /label=CheRiff
     CDS             1583..1636
                     /vntifkey="4"
                     /label=T2A
     misc_feature    2348..2350
                     /vntifkey="21"
                     /label=mScarlet-I
     misc_feature    1643..2347
                     /vntifkey="21"
                     /label=mCherry
BASE COUNT     1250 a      1462 c      1492 g      1333 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgaa gatgctgcac acttgtgggc tagcccttat 
      181 gtatcataca catacgattt aggtgacact atagaactcg agcagctgaa gcttcggagt 
      241 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gtcgactcta 
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagtc 
      361 gactctagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct 
      421 ccgagtcgac tctagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta 
      481 ctgtcctccg gctagtagcg gagactctag agggtatata atggatcccc gggtaccggt 
      541 cgccaccatg ggcggagctc ctgctccaga cgctcacagc gccccacctg gaaacgattc 
      601 tgccggaggc agtgagtacc atgccccagc tggatatcaa gtgaatccac cctaccaccc 
      661 cgtgcatggg tatgaggaac agtgcagctc catctacatc tactatgggg ccctgtggga 
      721 gcaggaaaca gctaggggct tccagtggtt tgccgtgttc ctgtctgccc tgtttctggc 
      781 tttctacggc tggcacgcct ataaggccag cgtgggatgg gaggaagtgt acgtgtgctc 
      841 cgtggagctg atcaaagtga ttctggagat ctatttcgag ttcaccagtc ctgctatgct 
      901 gttcctgtac ggagggaaca ttaccccatg gctgagatat gccgaatggc tgctgacatg 
      961 tcccgtgatc ctgattcatc tgtctaacat caccggcctg agtgaggcat acaataagcg 
     1021 gacaatggct ctgctggtgt ccgacctggg aactatttgc atgggagtga cagccgctct 
     1081 ggccactggg tgggtgaagt ggctgtttta ctgtatcggc ctggtgtatg gaacccagac 
     1141 attctacaac gctggaatca tctacgtgga gtcttactat atcatgcctg ccggcggctg 
     1201 taagaaactg gtgctggcca tgactgccgt gtactattct agttggctga tgtttcccgg 
     1261 cctgttcatc tttgggcctg aaggcatgca caccctgagc gtggctgggt ccactattgg 
     1321 ccataccatc gccgacctgc tgtccaagaa tatttgggga ctgctggggc acttcctgcg 
     1381 gatcaaaatt cacgagcata tcattatgta cggcgatatc aggagaccag tgagctccca 
     1441 gtttctggga cgcaaggtgg acgtgctggc cttcgtgaca gaggaagata aagtggcggc 
     1501 cgccaagagc aggatcacca gcgagggcga gtacatcccc ctggaccaga tcgacatcaa 
     1561 cgtggctcga gataggcgcg ccgagggcag aggaagtctg ctaacatgcg gtgacgtcga 
     1621 ggagaatcct ggcccaacta gtgtgagcaa gggcgaggag gataacatgg ccatcatcaa 
     1681 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 
     1741 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 
     1801 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 
     1861 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 
     1921 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 
     1981 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 
     2041 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 
     2101 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 
     2161 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 
     2221 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 
     2281 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 
     2341 gtacaagtaa gaattccgct cgagataatc aacctctgga ttacaaaatt tgtgaaagat 
     2401 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
     2461 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
     2521 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 
     2581 ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg 
     2641 ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta 
     2701 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 
     2761 gttcaggggg agatgtggga ggttttttaa agcggccgca ggaaccccta gtgatggagt 
     2821 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     2881 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 
     2941 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 
     3001 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     3061 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     3121 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     3181 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 
     3241 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     3301 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 
     3361 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     3421 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 
     3481 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 
     3541 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     3601 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 
     3661 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     3721 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     3781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     3841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     3901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     3961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     4021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     4081 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     4141 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     4201 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     4261 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     4321 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     4381 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     4441 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     4501 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     4561 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     4621 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     4681 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     4741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     4801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     4861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     4921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     4981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     5041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     5101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     5161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     5221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     5281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     5341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     5401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     5461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     5521 gccttttgct cacatgt 
//
