LOCUS       DuetHis   5368 bp    DNA   circular            Jan 2004
DEFINITION  DuetHis
COMMENT     Made in lab by G.V.; based on pETDuet
COMMENT     C-terminal His6 cloned in XhoI-AvrII sites
COMMENT     Restriction sites identical to pETDuet
FEATURES             Location/Qualifiers
     RBS             58..63
                     /dnas_title="RBS-1"
                     /vntifkey="32"
                     /label=RBS-1
     -35_signal      complement(3018..3023)
                     /dnas_title="-35 Ap"
                     /vntifkey="48"
                     /label=-35 Ap
     -10_signal      complement(2994..3000)
                     /dnas_title="-10 Ap"
                     /vntifkey="47"
                     /label=-10 Ap
     rep_origin      1523..1970
                     /dnas_title="f1 ori"
                     /vntifkey="33"
                     /label=f1 ori
     rep_origin      3713..3713
                     /dnas_title="ColE1, pBR322 ori"
                     /vntifkey="33"
                     /label=ColE1, pBR322 ori
     CDS             complement(4144..4335)
                     /dnas_title="ROP"
                     /vntifkey="4"
                     /label=ROP
     CDS             complement(4907..5989)
                     /dnas_title="lacI"
                     /vntifkey="4"
                     /label=lacI
                     /note="lac repressor gene"
     promoter        6380..6396
                     /dnas_title="T7 promoter"
                     /vntifkey="30"
                     /label=T7 promoter
     protein_bind    5..25
                     /dnas_title="lacO"
                     /vntifkey="31"
                     /label=lacO
     terminator      1438..1485
                     /dnas_title="T7 terminator"
                     /vntifkey="43"
                     /label=T7 terminator
     CDS             complement(2092..2952)
                     /dnas_title="Ap"
                     /vntifkey="4"
                     /label=Ap
                     /note="B-lactamase gene"
     primer          6334..6349
                     /dnas_title="pET Upstream Primer #69214-3"
                     /vntifkey="27"
                     /label=pET Upstream Primer #69214-3
     primer          complement(1424..1442)
                     /dnas_title="T7 Terminator Primer #69337-3"
                     /vntifkey="27"
                     /label=T7 Terminator Primer #69337-3
     CDS             71..1375
                     /note="HIV2 RT "
     source          1..6396
                     /dnas_title="pGV3340"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atggCGGTAG CAAAGGTCGA GCCCATTAAG ATCACCCTTA AGCCGGGGAA
      121 AGATGGCCCC AAGTTGCGTC AATGGCCCCT TACCAAAGAG AAGATCGAGG CTTTAAAGGA
      181 AATTTGCGAG AAGATGGAGA AAGAAGGGCA GTTGGAAGAA GCCCCGCCAA CGAATCCTTA
      241 TAACACCCCC ACATTCGCTA TTAAGAAGAA GGACAAGAAC AAGTGGCGTA TGCTGATCGA
      301 CTTCCGCGAA CTTAACAAAG TAACTCAGGA TTTTACGGAG ATCCAGCTGG GAATTCCCCA
      361 TCCAGCTGGT TTGGCGAAGA AGCGTCGTAT TACGGTGCTT GATGTTGGAG ACGCGTATTT
      421 CTCCATCCCG CTGCATGAAG ACTTCCGCCC ATACACCGCC TTTACGCTGC CGTCCGTCAA
      481 CAACGCTGAG CCCGGGAAAC GTTATATTTA CAAAGTCTTG CCGCAGGGGT GGAAAGGTAG
      541 TCCTGCCATC TTTCAACATA CAATGCGCCA GATTCTTGAA CCGTTTCGTA AGGCGAACAA
      601 GGACGTGATT ATCATTCAGT ACGTGGATGA CATTCTTATC GCGAGCGACC GCACAGATCT
      661 TGAACATGAT CGCGTTATCC TGCAACTTAA AGAATTGCTT AATAATCTGG GCTTCTCCAC
      721 TCCTGACGAG AAGTTCCAGA AAGATCCTCC TTATCACTGG ATGGGTTACG AGCTGTGGCC
      781 AACCAAATGG AAGTTACAGA AGATCCAGCT GCCCCAGAAG GAGGTTTGGA CCGTAAACGA
      841 CATTCAGAAG TTAGTAGGTG TATTAAACTG GGCCGCTCAA TTATATCCTG GTATTAAGAC
      901 GAAACATCTT TGTCGTCTGA TCCGCGGCAA GTTGACACTG ACTGAAGAAG TTCAATGGAC
      961 AGAGCTCGCT GAAGCAGAGC TTGAGGAGAA TCGCATCATC TTGTCACAGG AACAAGAGGG
     1021 GCATTACTAT CAGGAGGAGA AGGAACTGGA AGCTACTGTG CAGAAGGACC AGGACAACCA
     1081 ATGGACGTAC AAGATTCACC AGGAAGATAA GATTTTGAAG GTTGGAAAGT ATGCCAAGGT
     1141 GAAGAATACG CACACCAATG GTATTCGCTT GTTGGCGCAG GTTGTCCAGA AGATCGGCAA
     1201 GGAAGCCCTT GTAATTTGGG GACGTATCCC AAAATTTCAT CTGCCTGTTG AACGCGATAC
     1261 TTGGGAACAG TGGTGGGACA ACTACTGGCA AGTGACTTGG ATACCCGACT GGGACTTCGT
     1321 GTCGACACCT CCTCTGGTTC GTCTGGCTTT CAATCTTGTA AAGGACCCGA TTCCGggtac
     1381 cctcgagCAT CACCATCACC ATCACTAAcc taggctgctg ccaccgctga gcaataacta
     1441 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     1501 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1561 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1621 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1681 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1741 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1801 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1861 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1921 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg
     1981 gcggcacgat ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2041 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2101 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2161 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2221 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2281 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2341 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2401 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2461 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2521 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2581 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2641 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2701 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2761 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2821 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2881 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2941 ttgaatactc atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc
     3001 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa
     3061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3241 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3481 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3781 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3841 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3901 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca
     3961 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     4021 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     4081 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4141 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     4201 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     4261 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     4321 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     4381 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     4441 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     4501 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     4561 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     4621 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     4681 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     4741 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat
     4801 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     4861 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     4921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4981 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     5041 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     5101 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     5161 cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa
     5221 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     5281 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     5341 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     5401 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     5461 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     5521 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     5581 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     5641 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     5701 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     5761 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     5821 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     5881 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     5941 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     6001 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     6061 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     6121 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     6181 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     6241 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     6301 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     6361 gatctcgatc ccgcgaaatt aatacgactc actata
//

