LOCUS       Exported                7185 bp ds-DNA     circular SYN 20-AUG-2019
DEFINITION  Transformation vector pFZZ-neo3 DNA, complete sequence.
ACCESSION   AB570111
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Kataoka K, Schoeberl UE, Mochizuki K.
  TITLE     Modules for C-terminal epitope tagging of Tetrahymena genes
  JOURNAL   J. Microbiol. Methods 82 (3), 342-346 (2010)
  PUBMED    20624430
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Kataoka K, Mochizuki K.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2010) Contact:Kazufumi Mochizuki Institute of 
            Molecular Biotechnology of the Austrian Academy of Sciences (IMBA); 
            Dr. Bohr-Gasse 3, Vienna A-1030, Austria
REFERENCE   3  (bases 1 to 7185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 27, 2019 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..2240
                     /label=pBlueScript SK(+)
                     /note="pBlueScript SK(+)"
     primer_bind     complement(2218..2253)
                     /label=Vector_YU3_Primer_R
     primer_bind     2227..2282
                     /label=Q22YU3_5'UTR_F
     misc_feature    2247..3206
                     /label=Q22YU3_5'UTR
     primer_bind     3144..3177
                     /label=Q22YU3_5'UTR_For
     primer_bind     complement(3164..3219)
                     /label=Q22YU3_5'UTR_R
     primer_bind     3190..3240
                     /label=Vector_YU3_FLAG_F
     misc_feature    3207..3272
                     /gene="FLAG"
                     /label=FLAG
     misc_feature    3276..3296
                     /label=TEV protease recognition site
                     /note="TEV protease recognition site"
     misc_feature    3306..3680
                     /label=ZZ domain of protein A codon-optimized for Tetrahy
                     /note="ZZ domain of protein A codon-optimized for 
                     Tetrahymena"
     misc_feature    3687..4117
                     /label=BTU1 3' flanking
                     /note="BTU1 3' flanking"
     misc_feature    4118..4982
                     /label=MTT1_5'
     primer_bind     complement(4974..5005)
                     /label=Y_Neo3R
     misc_feature    4983..6106
                     /label=neo3
                     /note="neo3"
     primer_bind     complement(6071..6120)
                     /label=Vector_YU3_Neo3_R
     primer_bind     6084..6148
                     /label=Q22YU3_3'UTR_F
     misc_feature    6107..6466
                     /label=Q22YU3_3'UTR
     primer_bind     complement(6151..6184)
                     /label=Q22YU3_3'UTR_Rev
     primer_bind     complement(6429..6485)
                     /label=Q22YU3_3'UTR_R
     primer_bind     6452..6495
                     /label=Vector_YU3_Primer_F
     misc_feature    6467..7185
                     /label=pBlueScript SK(+)(1)
                     /note="pBlueScript SK(+)"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagagc atgcgctagc ggatccgtca ttaaaaaatt aattattaaa aaattaaatc
     2281 cgaggcttga gaaaataatg aatcataata ttaaatctat cctaaaaatt atccctctta
     2341 ctttttttag gttatttaat ttgatctttc ttaaaacagc tttgattaca gaaaatagaa
     2401 gagaagaatt tatagctagt aaaaaacaaa attaattaaa agcctttatt taaatttagt
     2461 aacaaacaat cagccaaatt cttattaatc aaatctaccc agacagatcc ttaataaact
     2521 ctttctgaat taacattttt ataatttcgt tcaccctctt agtcatcagg aaaatttaat
     2581 ttaaaatcaa attaacaatt gattctaaaa tacatacaat taattgcttt ttaagatggc
     2641 taactttacc taactaaaaa aacttattaa gtatcttacc aaatattagc atcacttatt
     2701 tgccttttgc tgattgagca tttgaattaa atttctcaaa atagatttac ctttaaaata
     2761 aaattaaatg gtatttttaa caattaattt tctttgctga aaataaaatc aagaataaaa
     2821 accaatcaat gatcgaatga gctaagaata gcttagaaag ccttttaaaa ttaaaatcat
     2881 agtatgcagc ttaagtatct tgagaaccac tttacaatag ttattatcat aataagatta
     2941 agaaaagaag tttaactctc tattatttat aacacccaac caaacctttt attataaaaa
     3001 atcaacatta taattaaata taatctctaa agcatcgctt gatattattg tctgtaaatt
     3061 tgtgtatatt tattaacctt cctattgtgc atatgagttt ttatagtttt taatttatat
     3121 tcataaataa atattactaa aatcattttt tttttttcat aaacaaaaat tatttccaaa
     3181 aataaattaa ttttataaca atcatagatt acaaagatca tgatggtgat tacaaagatc
     3241 acgatattga ttacaaagat gatgatgata aagctgaaaa tctttatttt caaggtgctt
     3301 ctgctcaaca tgatgaagct gttgataaca aatttaataa ggaacaacaa aatgcttttt
     3361 acgaaatttt acatcttcct aatttaaatg aagaacaaag aaatgctttc attcaatcat
     3421 taaaagatga tccttcacaa tctgctaatt tattagctga agctaaaaaa ttaaatgatg
     3481 ctcaagctcc taaagttgat aataaattca acaaagaaca acaaaacgct ttttatgaaa
     3541 ttcttcattt acctaacctt aatgaagaac aaagaaacgc tttcattcaa tctttaaagg
     3601 atgatccttc tcaatctgct aatttacttg ctgaagctaa aaagttaaat ggtgctcaag
     3661 ctcctaaggt tgatgctaat actagttgag cgaactgaat cggtcagcta aaccaaccaa
     3721 tcaacataat aaactttatt atttttactt aagcatctta ctgttgttgt aatagtagag
     3781 aaagaaatac ccaattaact tcattcacat aacattaata tctataaaca tcttttttct
     3841 cacatatata caactctcta aatcaacaaa taacttttta aaaataatgg atatatatta
     3901 acaaaataat atatctcttt ttacaaaata gttcttatat aaatacgtat tctgcactca
     3961 cccgcatttt tcacaacaaa aacataccaa aaaaattctt acttctacat gtttcctttc
     4021 ttattattac aaaattattt tataaatagc atacaaaaat aaatacaata aaaaaataaa
     4081 caaaatccag atcccccggg ctgcaggaat tcgatagaaa aatatttaaa aataaaaaat
     4141 aataagggtt ttgaataact cctttaattt aaatacacat ttttaaattt tttttagctc
     4201 tttaaatatt cataaaaata aaaaataact aactaaaaat aaataaaaag ataataatga
     4261 ttaaaggtat aatactgaat aagaaaaaac ataatagagt acttattttt tatatcacta
     4321 tttttaatat cttgaaagca aaactttttt atatatctta aaatatattg tatcgtttat
     4381 tcaattattt tctttaaatt tcaaatatat tgataaaaaa gatgacatgt tttttaaaga
     4441 aaacatgaaa tataaaatag ataaatatca attattttat ttattaaata tataagctgc
     4501 tcaaaacata gctcattcat caattataat atgtgaatca ttaattttca aaatattact
     4561 cattatttag gctatcattt attttttatt ttcaattatc cgtttctatt atattttaat
     4621 attaagttgt gattcttgaa ttttgtgtca tgaattattt gtaaatcttt ttatttctga
     4681 taaaaaatat aaattgattg actcatgatt taaatcatga gtcaacctaa ctaattttca
     4741 aaattcttct attctaaaat atagatgtga ttcttgaatc tctcttgaat ataaagtaat
     4801 tttttatatt tctgatataa ttcttagcta cgtgattcac gatttatgca atgatccata
     4861 taaaataatg taaatagtgt atatatatat attcgtcttt tttattcttt atataattta
     4921 aaaaaattaa aaaaatttaa taaagctcta ataaaataaa taataatact aaacttaaaa
     4981 taatgaagct tggatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5041 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5101 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5161 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5221 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5281 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5341 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5401 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5461 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5521 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5581 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5641 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5701 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5761 gacgagttct tctgagatcc ttaaattaaa aattcaatat atattttaca acttcatata
     5821 aataaatata ttatataaaa ttaattttta gtgtattaat attaacatta aagcaccaaa
     5881 aaaacgtgtt aatatactac tataaaatat aatttattcc aaattgacta aaatcattat
     5941 tttacaactc atttgtatat atattttatg tcaattattt tttttaactt tctaaaaaaa
     6001 aaaattcctc ttcacataca tgttagctct taaaaatttg tctgcaaatc caataataat
     6061 attttttttt gccattaaat tttcaaattt ttactggaaa aatgcaaaat taataattaa
     6121 ataaaattag aaaaagatga atttattgaa gaatatttaa agtaagaaga agtaagaata
     6181 aaccaaatta atgaacagtt gtaataagaa tgggaaacat ggaaataagt ttatgataag
     6241 attcatttag acaagtgatt aattataaat attaatattt aaactgttca atatttgtct
     6301 caagaacttg ttattttata cctttaagat tttaatcatt taatttatta aaagcttcca
     6361 atgaaataag tcttaattta attttatctt gcatgaaata tttaattcaa ataaaaggag
     6421 tttaatttca tttaaatgtt tatttagatt acttaattta tagagtgccc gggggatctg
     6481 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt acccaattcg
     6541 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     6601 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6661 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6721 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6781 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6961 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7021 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7081 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7141 tttaacgcga attttaacaa aatattaacg cttacaattt aggtg
//
