LOCUS       1309_pAAV_hSyn_d        5934 bp ds-DNA     circular     14-APR-2021
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(800..819)
                     /label=EGFP
                     /ApEinfo_fwdcolor=#37ff1d
                     /ApEinfo_revcolor=#37ff1d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    2679..3157
                     /vntifkey="25"
                     /label=hGH\polyA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1534..1912)
                     /label=Histone H2B
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3197..3337
                     /vntifkey="21"
                     /label=R-ITR
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(820..1515)
                     /label=EGFP(1)
                     /ApEinfo_label=EGFP
                     /ApEinfo_fwdcolor=#37ff1d
                     /ApEinfo_revcolor=#37ff1d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3429..3735
                     /vntifkey="33"
                     /label=f1\Origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4254..5111
                     /vntifkey="21"
                     /label=AmpR
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      5262..5929
                     /vntifkey="33"
                     /label=pUC\ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2051..2659
                     /vntifkey="21"
                     /label=WPRE
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    671..704
                     /vntifkey="21"
                     /label=lox2722
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1919..1952)
                     /vntifkey="21"
                     /label=lox2722(1)
                     /ApEinfo_label=lox2722
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    755..788
                     /vntifkey="21"
                     /label=loxP
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2003..2036)
                     /vntifkey="21"
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    156..640
                     /vntifkey="21"
                     /label=Human\Synapsin\promoter
                     /ApEinfo_fwdcolor=#ffa21b
                     /ApEinfo_revcolor=#fffa84
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt
      661 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg
      721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
      781 gaagttatgg cgcgccttaC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG
      841 TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG
      901 ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT
      961 TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA
     1021 AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT
     1081 AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA
     1141 GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT
     1201 TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT
     1261 TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTAGG
     1321 TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT
     1381 TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT
     1441 GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT
     1501 CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCTTAGC GCTGGTGTAC TTGGTGATGG
     1561 CCTTAGTACC CTCGGACACG GCGTGCTTGG CCAACTCCCC AGGCAGCAGC AGGCGCACGG
     1621 CCGTCTGGAT CTCCCTGGAG GTGATGGTCG AGCGCTTGTT GTAATGCGCC AGGCGGGAAG
     1681 CCTCACCTGC GATGCGCTCG AAAATGTCGT TCACAAACGA ATTCATGATG CCCATGGCCT
     1741 TGGACGAAAT GCCGGTGTCA GGGTGGACCT GCTTCAGAAC CTTGTACACA TAGATGGAAT
     1801 AGCTCTCCTT GCGGCTGCGC TTGCGCTTCT TGCCGCCTTT CTTCTGCGCC TTAGTCACCG
     1861 CCTTCTTGGA GCCCTTTTTC GGGGCGGGAG CAGACTTCGC TGGCTCTGGC ATgctagcat
     1921 aacttcgtat aaagtatcct atacgaagtt atttgcctta acccagaaat tatcactgtt
     1981 attctttaga atggtgcaaa gaataacttc gtataatgta tgctatacga agttatgaat
     2041 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2101 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2161 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2221 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2281 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2341 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2401 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2461 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
     2521 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     2581 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     2641 cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc
     2701 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     2761 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     2821 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     2881 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     2941 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     3001 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     3061 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     3121 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     3181 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     3241 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     3301 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     3361 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3421 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3481 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3541 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3601 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3661 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3721 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     3781 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3841 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     3901 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3961 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4021 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4081 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4141 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4261 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4321 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4381 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4441 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4501 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4561 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4621 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4681 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4741 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4801 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4861 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4921 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4981 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5041 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5101 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5161 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5221 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5401 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5881 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
