LOCUS       1364_pAAV2_U6gRN        5982 bp ds-DNA     circular     14-APR-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(3370..4052)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(5576..5882)
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(5444..5899)
                     /label=M13 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    196..428
                     /label=hU6 promotor
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(3329..4002)
                     /label=pUC origin
                     /ApEinfo_fwdcolor=#7f7f7f
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(4150..4809)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5049..5077)
                     /label=AmpR promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     repeat_region   19..163
                     /label=AAV ITR
                     /ApEinfo_fwdcolor=#7e0070
                     /ApEinfo_revcolor=#7e006d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     repeat_region   complement(2979..3123)
                     /label=AAV ITR(1)
                     /ApEinfo_label=AAV ITR
                     /ApEinfo_fwdcolor=#7e0070
                     /ApEinfo_revcolor=#7e006d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1050..2162
                     /label=H2B cherry
                     /ApEinfo_fwdcolor=#ff3657
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            569..1023
                     /label=Synapsin1 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     2174..2783
                     /label=WPRE
                     /ApEinfo_fwdcolor=#48ffb5
                     /ApEinfo_revcolor=#48ffb5
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     2810..2964
                     /label=SV40 pA
                     /ApEinfo_fwdcolor=#4bfff6
                     /ApEinfo_revcolor=#4bfff6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    457..538
                     /label=chimeric RNA backbone 1 (BsmBI)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    437..456
                     /label=gT28.13
                     /ApEinfo_fwdcolor=#ff455a
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 GGGGGGGGGG GGGGGGGGtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtGGC CAACTCCATC ACTAGGGGTT CCTCAGATCG GGCTGCAGGA
      181 ATTGGCCGCT CTAGAtttcc catgattcct tcatatttgc atatacgata caaggctgtt
      241 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
      301 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      361 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttGTGG
      421 AAAGGACGaa acacCGCGCA CTGGTTACAC ATGACCGTtt tagagctaGA AAtagcaagt
      481 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcTTTTTTTC
      541 TAGACTCTAG CTGCAGAGGG ACCTGCGTAT GAGTGCAAGT GGGTTTTAGG ACCAGGATGA
      601 GGCGGGGTGG GGGTGCCTAC CTGACGACCG ACCCCGACCC ACTGGACAAG CACCCAACCC
      661 CCATTCCCCA AATTGCGCAT CCCCTATCAG AGAGGGGGAG GGGAAACAGG ATGCGGCGAG
      721 GCGCGTGCGC ACTGCCAGCT TCAGCACCGC GGACAGTGCC TTCGCCCCCG CCTGGCGGCG
      781 CGCGCCACCG CCGCCTCAGC ACTGAAGGCG CGCTGACGTC ACTCGCCGGT CCCCCGCAAA
      841 CTCCCCTTCC CGGCCACCTT GGTCGCGTCC GCGCCGCCGC CGGCCCAGCC GGACCGCACC
      901 ACGCGAGGCG CGAGATAGGG GGGCACGGGC GCGACCATCT GCGCTGCGGC GCCGGCGACT
      961 CAGCGCTGCC TCAGTCTGCG GTGGGCAGCG GAGGAGTCGT GTCGTGCCTG AGAGCGCAGT
     1021 CGAATTCAAG CTGCTAGCAG CTTGGTACCG CCACCATGCC TGAACCCTCT AAGTCTGCTC
     1081 CAGCCCCTAA AAAGGGTTCT AAGAAGGCTA TCACTAAGGC GCAGAAGAAG GATGGTAAGA
     1141 AGCGTAAGCG CAGCCGCAAG GAGAGCTATT CTATCTATGT GTACAAGGTT CTGAAGCAGG
     1201 TCCACCCCGA CACCGGCATC TCATCCAAGG CCATGGGGAT CATGAACTCC TTCGTCAACG
     1261 ACATCTTCGA GCGCATCGCG GGCGAGGCTT CTCGCCTGGC TCACTACAAT AAGCGCTCGA
     1321 CCATCACCTC CAGGGAGATT CAGACGGCTG TGCGCCTGCT GCTGCCTGGG GAGCTGGCTA
     1381 AGCATGCTGT GTCCGAGGGC ACTAAGGCAG TTACCAAGTA CACTAGCTCT AAGGATCCAC
     1441 CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA
     1501 TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG
     1561 AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG
     1621 GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT
     1681 ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA
     1741 AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT
     1801 CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG
     1861 ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG CGGATGTACC
     1921 CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC
     1981 ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG
     2041 CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG
     2101 AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGT
     2161 AAACTAGTGG ATCCGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG
     2221 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT
     2281 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC
     2341 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT
     2401 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA
     2461 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT
     2521 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA
     2581 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT
     2641 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC
     2701 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG
     2761 CCTCCCCGCC TGGAATTCGA GCTCGGTACA GCTTATCGAT ACCGTCGACT TCGAGCAACT
     2821 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA
     2881 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC
     2941 ATGTCTGGAT CGTCTAGCAT CGAAGATCCC CCGATCTGAG GAACCCCTAg tgatggagtt
     3001 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg
     3061 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     3121 caaCCCCCCC CCCCCCCCCC CTGCAGCCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG
     3181 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
     3241 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     3301 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     3361 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     3421 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     3481 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     3541 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA
     3601 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     3661 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     3721 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     3781 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
     3841 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     3901 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     3961 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     4021 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     4081 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     4141 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     4201 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     4261 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
     4321 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     4381 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     4441 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     4501 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     4561 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     4621 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     4681 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     4741 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     4801 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     4861 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     4921 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     4981 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     5041 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     5101 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
     5161 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
     5221 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA
     5281 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
     5341 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
     5401 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT AAACGTTAAT
     5461 ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC
     5521 GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT
     5581 CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA
     5641 ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG
     5701 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA
     5761 CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT
     5821 AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT
     5881 GCGCCGCTAC AGGGCGCGTC GCGCCATTCG CCATTCAGGC TACGCAACTG TTGGGAAGGG
     5941 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCTG CA 
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