LOCUS       1379_pAAV2_U6gRN        6342 bp ds-DNA     circular     14-APR-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(3730..4412)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(5936..6242)
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(5804..6259)
                     /label=M13 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(3689..4362)
                     /label=pUC origin
                     /ApEinfo_fwdcolor=#7f7f7f
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(4510..5169)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5409..5437)
                     /label=AmpR promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2531..3143
                     /vntifkey="21"
                     /label=WPRE
                     /ApEinfo_fwdcolor=#bac9ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    437..456
                     /label=gT28.3
                     /ApEinfo_fwdcolor=#ff5245
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     repeat_region   19..163
                     /label=AAV ITR
                     /ApEinfo_fwdcolor=#7e0070
                     /ApEinfo_revcolor=#7e006d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1129..1162
                     /vntifkey="21"
                     /label=lox2722
                     /ApEinfo_fwdcolor=#bac9ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     repeat_region   complement(3339..3483)
                     /label=AAV ITR(1)
                     /ApEinfo_label=AAV ITR
                     /ApEinfo_fwdcolor=#7e0070
                     /ApEinfo_revcolor=#7e006d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2399..2432)
                     /vntifkey="21"
                     /label=lox2722(1)
                     /ApEinfo_label=lox2722
                     /ApEinfo_fwdcolor=#bac9ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2483..2516)
                     /vntifkey="21"
                     /label=loxP
                     /ApEinfo_fwdcolor=#bac9ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     3170..3324
                     /label=SV40 pA
                     /ApEinfo_fwdcolor=#4bfff6
                     /ApEinfo_revcolor=#4bfff6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1263..2375)
                     /label=H2B cherry
                     /ApEinfo_fwdcolor=#ff2b34
                     /ApEinfo_revcolor=#ff1115
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1213..1246
                     /vntifkey="21"
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#bac9ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    196..428
                     /label=hU6 promotor
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    457..538
                     /label=chimeric RNA backbone 1 (BsmBI)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    569..1098
                     /label=synapsin
                     /ApEinfo_fwdcolor=#282aff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 GGGGGGGGGG GGGGGGGGtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtGGC CAACTCCATC ACTAGGGGTT CCTCAGATCG GGCTGCAGGA
      181 ATTGGCCGCT CTAGAtttcc catgattcct tcatatttgc atatacgata caaggctgtt
      241 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
      301 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      361 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttGTGG
      421 AAAGGACGaa acacCGGCAC TCCACACAAT AGCTAGGTtt tagagctaGA AAtagcaagt
      481 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcTTTTTTTC
      541 TAGACTCTAG CTGCAGAGGG ACCTGCGTat gagtgcaagt gggttttagg accaggatga
      601 ggcggggtgg gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc
      661 ccattcccca aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgaG
      721 GCGCGTGCGC ACTGCCAGCT TCAGCACCGC GGACAGTGCC TTCGCCCCCG CCTGGCGGCG
      781 CGCGCCACCG CCGCCTCAGC ACTGAAGGCG CGCTGACGTC ACTCGCCGGT CCCCCGCAAA
      841 CTCCCCTTCC CGGCCACCTT GGTCGCGTCC GCGCCGCCGC CGGCCCAGCC GGACCGCACC
      901 ACGCGAGGCG CGAGATAGGG GGGCACGGGC GCGACCATCT GCGCTGCGGC GCCGGCCCAG
      961 CCGGACCGCA CCACGCGAGG CGCGAGATAG GGGGGCACGG GCGCGACCAT CTGCGCTGCg
     1021 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc
     1081 tgagagcgca gtcgagaagg taccggatcc tctagagtcg actccggaat aacttcgtat
     1141 aggatacttt atacgaagtt atgcagaatg gtagctggat tgtagctgct attagcaata
     1201 tgaaacctct taataacttc gtatagcata cattatacga agttatggcg cgccatacta
     1261 gtTTACTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC
     1321 TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT GTTGACGTTG
     1381 TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG
     1441 TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG GGCGCCGTCC
     1501 TCGGGGTACA TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG CATTACGGGG
     1561 CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC GCCGTCCTGC
     1621 AGGGAGGAGT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT CACGCGCTCC
     1681 CACTTGAAGC CCTCGGGGAA GGACAGCTTC AAGTAGTCGG GGATGTCGGC GGGGTGCTTC
     1741 ACGTAGGCCT TGGAGCCGTA CATGAACTGA GGGGACAGGA TGTCCCAGGC GAAGGGCAGG
     1801 GGGCCACCCT TGGTCACCTT CAGCTTGGCG GTCTGGGTGC CCTCGTAGGG GCGGCCCTCG
     1861 CCCTCGCCCT CGATCTCGAA CTCGTGGCCG TTCACGGAGC CCTCCATGTG CACCTTGAAG
     1921 CGCATGAACT CCTTGATGAT GGCCATGTTA TCCTCCTCGC CCTTGCTCAC CATGGTGGCG
     1981 ACCGGTGGAT CCTTAGAGCT AGTGTACTTG GTAACTGCCT TAGTGCCCTC GGACACAGCA
     2041 TGCTTAGCCA GCTCCCCAGG CAGCAGCAGG CGCACAGCCG TCTGAATCTC CCTGGAGGTG
     2101 ATGGTCGAGC GCTTATTGTA GTGAGCCAGG CGAGAAGCCT CGCCCGCGAT GCGCTCGAAG
     2161 ATGTCGTTGA CGAAGGAGTT CATGATCCCC ATGGCCTTGG ATGAGATGCC GGTGTCGGGG
     2221 TGGACCTGCT TCAGAACCTT GTACACATAG ATAGAATAGC TCTCCTTGCG GCTGCGCTTA
     2281 CGCTTCTTAC CATCCTTCTT CTGCGCCTTA GTGATAGCCT TCTTAGAACC CTTTTTAGGG
     2341 GCTGGAGCAG ACTTAGAGGG TTCAGGCATG GTGGCGGTAC CGGTGGATCC TTgctagcat
     2401 aacttcgtat aaagtatcct atacgaagtt atttgcctta acccagaaat tatcactgtt
     2461 attctttaga atggtgcaaa gaataacttc gtataatgta tgctatacga agttatgaat
     2521 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2581 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2641 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2701 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2761 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2821 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2881 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2941 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
     3001 gctacgtccc ttcggccctc aatccagcgg accttccttc CCGCGGCCTG CTGCCGGCTC
     3061 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG
     3121 CCTCCCCGCC TGGAATTCGA GCTCGGTACA GCTTATCGAT ACCGTCGACT TCGAGCAACT
     3181 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA
     3241 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC
     3301 ATGTCTGGAT CGTCTAGCAT CGAAGATCCC CCGATCTGAG GAACCCCTAg tgatggagtt
     3361 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg
     3421 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     3481 caaCCCCCCC CCCCCCCCCC CTGCAGCCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG
     3541 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
     3601 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     3661 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     3721 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     3781 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     3841 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     3901 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA
     3961 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     4021 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     4081 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     4141 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
     4201 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     4261 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     4321 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     4381 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     4441 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     4501 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     4561 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     4621 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
     4681 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     4741 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     4801 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     4861 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     4921 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     4981 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     5041 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     5101 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     5161 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     5221 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     5281 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     5341 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     5401 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     5461 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
     5521 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
     5581 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA
     5641 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
     5701 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
     5761 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT AAACGTTAAT
     5821 ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC
     5881 GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT
     5941 CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA
     6001 ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG
     6061 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA
     6121 CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT
     6181 AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT
     6241 GCGCCGCTAC AGGGCGCGTC GCGCCATTCG CCATTCAGGC TACGCAACTG TTGGGAAGGG
     6301 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCTG CA 
//
