LOCUS       pPCT62_TS1.1_212_addgen 9726 bp ds-DNA     circular     14-APR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        263..826
                     /label="pU6 5'CMV promoter"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
     LTR             844..1024
                     /label="LTR 5'"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1695..1928
                     /label="RRE"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     LTR             2459..2473
                     /label="cPPT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2631..2920
                     /label="bU6-2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2921..2941
                     /label="whtB (perfect)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2942..3034
                     /label="sgRNA F + E constant region"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        3055..3368
                     /label="mouse U6 - modified"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3249..3282
                     /label="LoxP"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3369..3388
                     /label="site1:Bao_ADE2.d"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3389..3483
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        3503..3778
                     /label="hU6 promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3780..3800
                     /label="bri1 (perfect)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3801..3895
                     /label="sgRNA_constant_region"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        3953..5124
                     /label="ef1a"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     gene            5136..5732
                     /label="PURO"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5733..5795
                     /label="T2A"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     gene            5808..6503
                     /label="BFP"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_recomb     6526..6559
                     /label="LoxP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     LTR             6605..7205
                     /label="wPRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     LTR             7395..7409
                     /label="cPPT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     LTR             7732..7912
                     /label="3' LTR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(7956..8638)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(8736..9395)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        9635..9663
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_fwdcolor=#346ee0
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga CGTGACCGAG
     2641 CTTGTCTGCC CGCGCAGTCC ACTAGACAGA CGCGCAGAGG TCGGGCGGGC CTAGGGCCAA
     2701 TTTCCCATGG TTCCCTTCAT TTGCATATAT GATGTAATAA GGTTATGGAG ACTATTAAAC
     2761 TTAGCCCTAA TCAAACTATA TGATGATAAT GTGTGTGGTA CAAAAGGTCA TAACTTATTA
     2821 TATACTTTGA AACTTAAAAA AGGGTTACAG TTTAGTCACC ATAACTGTAA AATTTTTTCT
     2881 ATTCTTAGCT TTATATAGTT CTTGAGAGGC CATGTTTATG GCAGGAGCTA TCAATTCGCG
     2941 GGTTTaAGAG CTAaGCTGGA AACAGCATAG CAAGTTtAAA TAAGGCTAGT CCGTTATCAA
     3001 CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTCCAGTC TGACTATCAC GATGgatccg
     3061 acgcgccatc tctaggcccg cgccggcccc ctcgcacgga cttgtgggag aagctcggct
     3121 actcccctgc cccggttaat ttgcatataa tatttcctag taactataga ggcttaatgt
     3181 gcgataaaag acagataatc tgttcttttt aatactagct acattttaca tgataggctt
     3241 ggatttctat aacttcgtat agcatacatt atacgaagtt ataaacagca caaaaggaaa
     3301 ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaGtatccct tggagaaCCA
     3361 cctTGTTGGT CCTTTGTATG CCGAAAAAGT TTGAGAGCTA AGCAGAAAGC TGCATAGCAA
     3421 GTTCAAATAA GGCTAGTCCG TACACAACTT GAAAAAGTGG CAGCCGAGTC GGCTGCTTTT
     3481 TTTGCTCGAA TCTACACTCA GCTATGGCGC GCCCCAAGGT CGGGCAGGAA GAGGgcctat
     3541 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     3601 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     3661 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     3721 taacttgaaa gtatttcgat ttcttggctt tatatatctt gTGGAAAGcc aGAAACAtgG
     3781 TTGGGTCATA ACGATACCTC GTTTCAGAGC TAAGCACAAG AGTGCATAGC AAGTTGAAAT
     3841 AAGGCTAGTC CGTTTACAAC TTGAAAAAGT GGCACCCGAG TCGGGTGCTT TTTTTCtcga
     3901 gtactaggat ccattaggcg gccgcgtgga taaccgtatt accgccatgc atGTGCCCGT
     3961 CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT
     4021 TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG
     4081 CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC
     4141 GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA GTGCCGTGTG TGGTTCCCGC
     4201 GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT GAATTACTTC CACCTGGCTG
     4261 CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG TTCGAGGCCT
     4321 TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC
     4381 CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA
     4441 GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA
     4501 AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG
     4561 GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA
     4621 ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG
     4681 TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG CACCAGTTGC GTGAGCGGAA
     4741 AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT GGAGGACGCG GCGCTCGGGA
     4801 GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT
     4861 TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC TCGATTAGTT CTCGcGCTTT
     4921 TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT
     4981 GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA TGTAATTCTC CTTGGAATTT
     5041 GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT TCAAAGTTTT
     5101 TTTCTTCCAT TTCAGGTGTC GTGAgCTAGC CCACCATGAC CGAGTACAAG CCCACGGTGC
     5161 GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG
     5221 ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC
     5281 TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG
     5341 ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG
     5401 CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA
     5461 TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG
     5521 GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG
     5581 AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC
     5641 CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC
     5701 GCACCTGGTG CATGACCCGC AAGCCCGGTG CCGGATCGGG AGAGGGCAGA GGAAGTCTGC
     5761 TAACATGCGG TGACGTCGAG GAGAATCCTG GCCCAccggt cgccaccAGC GAGCTGATTA
     5821 AGGAGAACAT GCACATGAAG CTGTACATGG AGGGCACCGT GGACAACCAT CACTTCAAGT
     5881 GCACATCCGA GGGCGAAGGC AAGCCCTACG AGGGCACCCA GACCATGAGA ATCAAGGTGG
     5941 TCGAGGGCGG CCCTCTCCCC TTCGCCTTCG ACATCCTGGC TACTAGCTTC CTCTACGGCA
     6001 GCAAGACCTT CATCAACCAC ACCCAGGGCA TCCCCGACTT CTTCAAGCAG TCCTTCCCTG
     6061 AGGGCTTCAC ATGGGAGAGA GTCACCACAT ACGAAGACGG GGGCGTGCTG ACCGCTACCC
     6121 AGGACACCAG CCTCCAGGAC GGCTGCCTCA TCTACAACGT CAAGATCAGA GGGGTGAACT
     6181 TCACATCCAA CGGCCCTGTG ATGCAGAAGA AAACACTCGG CTGGGAGGCC TTCACCGAGA
     6241 CGCTGTACCC CGCTGACGGC GGCCTGGAAG GCAGAAACGA CATGGCCCTG AAGCTCGTGG
     6301 GCGGGAGCCA TCTGATCGCA AACATCAAGA CCACATATAG ATCCAAGAAA CCCGCTAAGA
     6361 ACCTCAAGAT GCCTGGCGTC TACTATGTGG ACTACAGACT GGAAAGAATC AAGGAGGCCA
     6421 ACAACGAGAC CTACGTCGAG CAGCACGAGG TGGCAGTGGC CAGATACTGC GACCTCCCTA
     6481 GCAAACTGGG GCACAAGCTT AATtaagaat tcgtcgaggg acctaataac ttcgtatagc
     6541 atacattata cgaagttata catgtttaag ggttccggtt ccactaggta caattcgata
     6601 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6661 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6721 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6781 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6841 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6901 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6961 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7021 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7081 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7141 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7201 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     7261 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7321 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7381 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7441 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     7501 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     7561 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     7621 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     7681 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     7741 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7801 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7861 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
     7921 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7981 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8041 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8101 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8161 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8221 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8281 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8341 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8401 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8461 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8521 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8581 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8641 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8701 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8761 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8821 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8881 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8941 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9001 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9061 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9121 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9181 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9241 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9301 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9361 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9421 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9481 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9541 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9601 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9661 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9721 cctgac
//