LOCUS       Exported                6737 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     62..81
                     /label=T7
     primer_bind     101..140
                     /label=CVP434
     primer_bind     111..169
                     /label=CVP679
     misc_feature    142..1425
                     /label=YebS
     CDS             142..1425
                     /label=Translation 142-1425
     misc_feature    142..169
                     /label=29 / 59.7
     primer_bind     589..607
                     /label=oDCE3554
     misc_feature    840
                     /label=T->C mutation relative to reference sequence
     misc_feature    843
                     /label=T->A mutation relative to reference sequence
     primer_bind     1068..1092
                     /label=oDCE3209
     misc_feature    1113
                     /label=A->C mutation relative to reference sequence
     primer_bind     1195..1215
                     /label=oDCE3555
     primer_bind     1328..1349
                     /label=CVP673
     misc_feature    1394..1425
                     /label=YebT
     CDS             1394..1425
                     /label=Translation 1394-1425
     CDS             1426..3691
                     /label=MCE domain protein
     CDS             1426..3691
                     /label=yebT
     CDS             1427..3691
                     /label=Translation 1427-3691
     misc_feature    1451..1513
                     /label=TM helix (TOPCONS)
     misc_feature    1526..1804
                     /label=MCE domain (CDD)
     primer_bind     1529..1558
                     /label=CVP676
     primer_bind     complement(1592..1614)
                     /label=oDCE3210
     primer_bind     complement(1596..1618)
                     /label=CVP675
     misc_feature    1868..2113
                     /label=MCE domain (weak/partial hit, CDD)
     misc_feature    1904..1906
                     /label=Silent mutation t->C from plasmid pBEL1620
     primer_bind     1963..1984
                     /label=CVP640
     misc_feature    2211..2230
                     /label=start2
     misc_feature    2228..2230
                     /label=MCE domain (CDD)
     misc_feature    2231..2467
                     /label=MCE domain (weak/partial hit, CDD)
     misc_feature    2231..2264
                     /label=mce4start
     STS             2364..2649
                     /label=STS
     primer_bind     complement(2640..2661)
                     /label=CVP752
     misc_feature    2954..3229
                     /label=MCE domain (CDD)
     misc_feature    3293..3550
                     /label=MCE domain (CDD)
     misc_feature    3476..3478
                     /label=Silent mutation to c->G from plasmid pBEL1620 f
                     /label=Silent mutation to c->G from plasmid pBEL1620 from 
                     Georgia
     primer_bind     complement(3668..3727)
                     /label=CVP681
     terminator      3771..3817
                     /label=T7 terminator
     primer_bind     complement(3850..3869)
                     /label=oDCE3649
     CDS             4251..4910
                     /label=AmpR
     primer_bind     complement(4586..4604)
                     /label=oDCE3209
     rep_origin      5008..5690
                     /label=ColE1 origin
     primer_bind     complement(5398..5456)
                     /label=CVP347
     primer_bind     5581..5604
                     /label=CVP669
     primer_bind     complement(5852..5873)
                     /label=oDCE3245
ORIGIN
        1 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatggctctt aacacaccac aaattacgcc gacaaaaaag
      181 ataacagtga gggcaatcgg cgaggaactg ccgcgtggtg attaccaacg ttgcccgcaa
      241 tgtgacatgc tgtttagcct gcccgagata aattctcatc aaagtgccta ttgtccgcgc
      301 tgtcaggcaa aaattcgtga cgggcgcgac tggtcgctaa cgcgcctggc ggcaatggcc
      361 ttcaccatgc tgttgcttat gccgtttgcc tggggcgaac cgctgttgca tatctggctg
      421 ttaggcattc gtattgacgc caacgttatg caaggcatct ggcaaatgac caaacagggc
      481 gatgcgataa cggggtcgat ggtctttttc tgcgttattg gtgccccgct cattctggtg
      541 acctccatag cttatttatg gtttggtaac cgactgggaa tgaatttacg tccggtactg
      601 ctgatgcttg agcgacttaa agagtgggta atgctcgata tctacctggt cggcattggc
      661 gttgcttcta taaaggtaca ggattatgcc catatccagg ctggtgtcgg cttgttctct
      721 tttgtggcgt tggtgatttt aacgacggtg acgttgtcac atcttaatgt cgaagaactg
      781 tgggagcgat tttatccgca gcgccccgct acgcgtaggg acgagaaact tcgtgtctgc
      841 ctagggtgcc attttaccgg ctatccagat cagcgtggtc gctgcccgcg ttgccatatc
      901 ccgctacgcc tgcgtcgccg tcatagtctg caaaaatgct gggcggcgct gttagcgtca
      961 atcgttttat tgttacctgc caacctgttg cctatttcta tcatttatct gaacggagga
     1021 cggcaggaag atacaattct ttccggaatt atgtcgctgg caagtagcaa cattgcggtt
     1081 gcaggaatcg tgtttatcgc cagtattctg gtcccgttta ctaaagtgat cgtcatgttc
     1141 actttactgt tgagcattca ttttaaatgc cagcaaggtt tacgcacacg cattctgtta
     1201 ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg acctgtttgt catatcttta
     1261 accatgtcgc tgattaatcg cgatcagatc ctcgctttta ctatgggacc ggctgcgttt
     1321 tatttcggcg cagcggtaat tttgactatt cttgctgtgg aatggctgga cagccgctta
     1381 ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg actgaagcgc agattaaaaa
     1441 taaacgccgt atctcacctt tctggctgct gccgttcatc gcgctaatga ttgccagttg
     1501 gctgatttgg gacagttatc aggaccgggg taataccgtc accatcgact ttatgtcggc
     1561 ggatggtatt gttccaggcc gtacgcctgt tcgttatcag ggcgttgaag tcggaacagt
     1621 gcaggatatc agcctcagcg acgatcttcg taagattgaa gtcaaggtca gcatcaagtc
     1681 cgatatgaaa gatgcgctgc gcgaagagac tcagttctgg ctggtgacgc caaaagcatc
     1741 gttggcaggt gtctccgggc tggacgccct cgtcggtggg aactatatcg gcatgatgcc
     1801 gggtaaaggt aaagagcagg atcactttgt cgcactcgat acccaaccga aatatcggct
     1861 ggacaatggc gatctgatga tccacctgca agcccccgat ctcggctcgc tgaacagcgg
     1921 ttcattggtc tatttccgca agatcccggt gggaaaagtc tacgactatg ccatcaatcc
     1981 caacaagcaa ggcgtggtga ttgatgtcct gatcgagcgg cgttttaccg atctggtgaa
     2041 aaaaggtagc cgtttctgga acgtttccgg cgttgatgcc aacgtcagta tcagtggcgc
     2101 gaaggtgaaa ctggaaagcc tggcggcact ggttaacggt gcgattgcct tcgattcacc
     2161 agaagagtcg aaacctgccg aggcggaaga tacctttggt ctgtatgaag atctggccca
     2221 cagccagcgt gttctgacgc tgaccctgac cgcgccggaa agttacggta ttgatgcggg
     2281 tcagccgctc attcttcacg gcgtgcaggt aggccaggtt atcgatcgta aactcaccag
     2341 caaaggcgtc acctttaccg tcgccatcga gcctcagcat cgcgaactgg taaaaggcga
     2401 tagcaaattt gtcgtcaaca gccgtgtcga tgtgaaggtg gggctggatg gcgttgagtt
     2461 tctcggtgcc agcgcctcag aatggatcaa tggcgggata cgtattctgc cgggcgataa
     2521 aggtgagatg aaagccagct atccactgta tgccaatttg gaaaaagcgc tggagaacag
     2581 ccttagcgat ttacccacca caaccgtgag tttgagtgca gagacgctgc cggatgtgca
     2641 ggcaggatcg gtagtgctgt accgtaaatt tgaagttggt gaagtgatta ccgtgcgtcc
     2701 gcgagctaac gcgtttgata tcgatctgca tattaagccg gagtatcgca accttctgac
     2761 cagcaatagc gtgttctggg cagaaggcgg ggcgaaagtt cagctgaatg gtagtggtct
     2821 gaccgtacag gcatccccgc tctccagagc attaaaggga gccattagct tcgacaacct
     2881 cagcggtgcc agcgccagtc agcgtaaagg cgataagcgt attctgtatg cttccgaaac
     2941 agcggcccgt gcggttggcg ggcagattac gcttcacgct ttcgatgccg gaaaactggc
     3001 ggtcgggatg ccaattcgct atctcggtat tgatatcggg caaatccaga cgctggatct
     3061 gattaccgca cgcaatgaag tacaggcaaa ggcggtactt tacccggaat atgtccagac
     3121 ctttgctcgc ggtggtacgc gcttctcagt ggtcacaccg caaatttcgg cagctggcgt
     3181 tgagcatctt gatactatcc tccagccgta tatcaacgtc gaaccaggcc ggggcaatcc
     3241 tcgccgcgac tttgaattac aagaggccac cattactgat tcgcgttacc tggatggctt
     3301 aagcattatt gttgaagcgc cggaagccgg ttcgttaggc atcggtacgc ctgtgctgtt
     3361 ccgtggtctg gaagtcggta cggttacagg aatgacgctg gggacattgt cagatcgcgt
     3421 gatgattgcg atgcgcatca gtaaacgcta tcaacacctg gtgcgtaaca attccgtgtt
     3481 ctggttggca tcgggttaca gtctggactt tggtctgacg ggcggcgtag tgaaaaccgg
     3541 cacctttaac cagtttatcc gtggcggcat cgccttcgcc acgcctccgg gtacgccact
     3601 ggcaccgaaa gcccaggaag gcaaacactt cctgttgcag gaaagtgaac cgaaagagtg
     3661 gcgtgaatgg ggaactgcgc ttcccaaata atgcccacct cgagcagatc cggctgctaa
     3721 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     3781 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
     3841 ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3901 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4261 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     4321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4561 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     4621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5221 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     5881 cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc
     5941 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     6001 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6061 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6121 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     6181 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     6241 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     6301 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     6361 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     6421 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     6481 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     6541 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     6601 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     6661 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     6721 gtcctcaacg acaggag
//
